LABORATORY EXPERIMENT 2

Student Name : Subject : Biology

Grade/Class : 11 Science/ Topic : Energy and Respiration
Date : November 8, 2021 Teacher(s) : Ms. Brigitta Novariska
Semester : 1 Parent’s Signature :
RESULT

Investigating the effect of temperature on respiration rate of germinating seeds

A. Guidelines and Instruction

1. This experiment will be done virtually.
2. You have to do the task individually.
3. If you use other sources outside the textbook to answer the questions, put the resource/s
link below your answer/s.
4. Write down your answer in Google Doc / MS Word, and turn it in through google
classroom before 9 PM on November 9, 2021.
5. Late submission will result in a deduction of 2 points/day and should not be more than 5
days. If so, the student will get 0.

B. Introduction
Respiration is the process during which simple carbohydrates, like glucose, break down
into simpler substances and liberate carbon dioxide and energy. The compound used, or
oxidized, during respiration is called a respiratory substrate. Carbohydrates, fats, and proteins
are examples of respiratory substrates, and carbohydrates are the preferred respiratory
substrate among them. The rate of respiration can be measured in terms of gas exchange, that
is consumption of the respiratory substrate oxygen or evolution of carbon dioxide. By respiration
rate we mean the uptake of oxygen per unit time.
Respiration, like most of the chemical reactions in living things, is controlled by enzymes
and so is affected by temperature. At a very high temperature, the rate of respiration decreases
with time, and at very low temperature, the respiration rate is insignificant. Respiration in plants
can be studied in moist germinating seeds that release carbon dioxide (CO2) during respiration. The
seeds are kept in an air tight conical flask. A small test tube containing potassium hydroxide (KOH)
solution is placed in the flask to absorb carbon dioxide released by the respiring germinating seeds.

MS HIGH | Grade 11 Science | Biology | Laboratory Experiment 2 | November 8, 2021 1

FR/MSCS/CRD/066-Rev00
C. Simulator Procedure
(as performed through the Online Labs)
1. Go to the laboratory simulator through this link:
http://amrita.olabs.edu.in/?sub=79&brch=17&sim=204&cnt=2
2. Investigate the rate of respiration of germinating mustard, bean, and wheat seeds.
3. Use 20 seeds for every experiment in 5, 15, 25, 35 oC.
4. To change the type of seed, select from the drop-down list, ‘Select the seeds’.
5. You can increase or decrease the number of seeds inside the beaker by moving the
‘Number of seeds’ slider accordingly.
6. Increase or decrease the temperature using the slider, ‘Temperature’.
7. Click on the ‘Start’ button to begin the experiment.
8. You can observe that the level of water rises in the delivery tube dipped in the beaker.
9. A timer is shown. Wait till the timer stops.
10. Measure the rise of water level in the delivery tube and enter the values in the test box,
‘Rising level of water (ml)’ on the left pane.
11. Click on the submit button to verify your result. (the correct result have green check mark)
12. Record the result in the table.
13. You can redo the experiment by clicking on the ‘Reset’ button.

D. Real Lab Materials and Equipment [5]

Write down materials and equipments needed if you do the experiment in the real lab.

____________________________________________________________________________

____________________________________________________________________________

E. Real Lab Procedures [10]

Write down the procedures if you do the experiment in the real lab.

____________________________________________________________________________

____________________________________________________________________________

____________________________________________________________________________

____________________________________________________________________________

____________________________________________________________________________

____________________________________________________________________________

____________________________________________________________________________

____________________________________________________________________________

____________________________________________________________________________

____________________________________________________________________________

MS HIGH | Grade 11 Science | Biology | Laboratory Experiment 2 | November 8, 2021 2

FR/MSCS/CRD/066-Rev00
F. Results
Table 1: Results of the rise of water level [6]
The rise of water level (ml)
Temperature (oC)
Mustard Seeds Beans Wheat Seeds
5
15
25
35

Graph 1: The rise of water level at various intervals of temperature [10]

(The graph should have correct x and y axis [@0.5], full labels including units on both axes
[@1], and all data from each kind of seed [@2])

G. Analysis, Conclusion, and Evaluation [14]

Read the statements below and type your answers on the space provided.
1. What is the function of potassium hydroxide solution in this experiment? [1]

2. Explain what does the rise of water level indicate. [1]

3. Why does the changes in water level can be used to measure the rate of respiration? [2]

4. Explain the changes on the respiration rate of each kind of germinating seeds (mustard,
bean, and wheat) during the interval of temperature. [3]

5. What is the optimum temperature for respiration of germinating seeds (you may refer to the
answer no. 4). Explain why do you think so. [3]

6. Explain the difference of water level rise on mustard, bean, and wheat seeds in optimum
temperature. Why do you think there is a difference result? [4]

Resources:
• amrita.olabs.edu.in. (2015). Rate of Respiration. Retrieved 2 November 2021, from
amrita.olabs.edu.in/?sub=79&brch=17&sim=204&cnt=4

MS HIGH | Grade 11 Science | Biology | Laboratory Experiment 2 | November 8, 2021 3

FR/MSCS/CRD/066-Rev00