Name: Joshua James Mendez 

              Date Performed:  April 12, 2021

Year and Course: 1st Year BSN   Date Submitted:  ____________

Subject title: Microbiology and Parasitology Code number: 9231

Name of Professor: Angeli Emborgo Score: ______________________

                                                                                                              Exercise no. 3

                                                                         Culture Media: Classification based on components and use

INTRODUCTION

Culture medium or growth medium is a liquid or gel designed to support the growth of

microorganisms. It contains nutrients, growth promoting factors, energy sources, buffer 

salts, minerals, metals, and gelling agents for solid media.

There are different types of media suitable for growing different types of cells.

OBJECTIVES:

At the end of the experiment, each student will be able to:

 a) know and understand the importance of Culture Media

b) Demonstrate knowledge on the different types of media.

c) Know and familiarize the different uses of culture media. 

MATERIALS

              Laboratory Manual

              Microbiology Notes

PROCEDURE

Identification and visual presentation of the different Culture Media

 I. Media are of different types on consistency and chemical composition. 

  a) Identification of the different types of Culture Media according to 

       consistency and chemical composition.

 
  b) Provide a picture of each type of Culture Media.

II. Importance and Uses of each Culture Media

   b) A review on the different types of culture media commonly used in 

       microbiology.

III. Post Laboratory Analysis

     a) Answer the questions related to the laboratory activity.

IV. ANALYSIS

     a) Why is it necessary to know the uses of Culture Media in 

          Microbiology?

 Microbial cultures are used to identify the type of organism, as well as its abundance in the sample
under investigation. It is one of the most popular microbiology diagnostic methods, and it is used to
determine the cause of infectious disease by allowing the agent to multiply in a predetermined medium.
The analysis of microorganism cultural characteristics is done with the aim of classifying different
microorganisms into different taxonomic classes. The aim of this experiment is to learn about different
microorganism characteristics and classify them into different classes based on those characteristics.

CULTURE TYPE OF  DESCRIPTION/

MEDIA  CULTURE USES
 PICTURE/VISUAL PRESENTATION
MEDIA
 
According a) Solid Solid medium has
to Media physical structure
Consistenc agar and allows bacteria
y to grow in
physically
informative or
useful way

b) Liquid Media Broth is a nutrient-

Broth infused liquid
medium used for
growing bacteria.

According 1) Routine Is composed of 6

to Chemical Laboratory types which are
Compositio media basal media,
n enriched media,
selective media,
indicator media,
transport media,
and storage media.

Types
a) Basal Basal Media
Media promotes the
growth of many
types of
microorganisms
which do not
require any special
nutrient
supplements.
b) Enriched Enriched media
media contain the
nutrients required
to support the
growth of a wide
variety of
organisms,
including some of
the more fastidious
ones.

c) Selective Selective media are

Media used for the growth
of only selected
microorganisms.
Are used to select
for the growth of a
particular
“selected”
microorganism.

d) Indicator An indicator media

Media is included in the
medium. A
particular organism
causes change in
the indicator

e) Transport Transport media

Media are special media
that are formulated
to keep a specimen
fresh and prevent
bacterial
overgrowth from
the time it is
collected to the
time it is sent to the
laboratory to be
processed.
 f) Storage Storage Media used
media for storing the
bacteria for a long
period of time.

Common
Media in
Routine
Use
a) Nutrition Nutrient broth is
Broth nutrient agar that
lacks agar powder,
which acts as a
solidifying agent.
They stay liquid at
room temperature
and are commonly
used to keep
microorganism
stocks alive. They're
usually used to
grow picky species.
b) Nutrient Nutrient agar is
Agar widely used
because it can
support the growth
of a wide range of
bacteria and fungi
and contains
several nutrients
needed for
bacterial growth.
c) Peptone Peptone is a
Agar protein-amino acid
mixture made from
the breakdown of
natural products
such as animal
tissues, milk, and
vegetables.
Peptone is used in
nutrient agar to
provide a protein
source for
microorganisms to
grow on.
d) Blood Agar Blood agar is a
bacterial growth
medium that is
enriched with
nutrients.
Streptococci and
other fastidious
species do not grow
well on standard
growth media.
e) Chocolate Chocolate agar is
Agar/ made up of blood
heated that has been
Blood agar heated to release
growth factors that
help fastidious
bacteria grow.

f) MacConkey Only gram-negative

Agar bacteria grow on
MacConkey agar,
and their
appearance varies
depending on their
lactose fermenting
potential, the rate
of fermentation,
and whether or not
a capsule is present.
g) Mueller Mueller-Hinton has
Hinton a number of
Agar characteristics that
make it an effective
antibiotic. Starch
has been shown to
absorb toxins
released by
bacteria, preventing
them from
interfering with
antibiotics. It's also
a loose agar. In
comparison to most
other plates, this
allows for greater
antibiotic diffusion.
f) Hiss’s Pneumococcus,
Serrum Neisseria, and
Water Corynebacterium
medium can't develop in
peptone water
sugar media, so this
Hiss's Serum Water
Medium is used to
research their
fermentation
reactions.

g) Lowentstei The Lowenstein–

n-Jensen Jensen medium (LJ
medium medium) is a
growth medium
designed
specifically for the
culture of
Mycobacterium
species, especially
Mycobacterium
tuberculosis. M.
tuberculosis occurs
as brown, granular
colonies when
grown on LJ
medium.
 h) Loeffler Loeffler Medium is
Medium a tweaked version
of Loeffler's original
formula from 1887.
Primary and
secondary isolation
and cultivation of
fastidious
pathogenic
microorganisms,
especially from the
nose and throat,
are enhanced by
Loeffler medium.
i) Tellurite Tellurite Blood Agar
Blood Agar is a selective
medium for
Corynebacterium
species isolation
and cultivation. It's
selective because of
the inhibitor, and
differential because
of the organism's
ability to reduce
potassium tellurite.
         

VI. CONCLUSION

Culture media are used for the general cultivation and maintenance of bacteria stored in laboratory
culture collections since they contain all of the elements that most bacteria need for growth and are not
selective. Most microbiological tests need culture media in order to obtain pure cultures, develop and
count microbial cells, and cultivate and pick microorganisms.