Experiment A - Noradrenaline: MEDSCI 305 Laboratory 2: Role of Calcium in Smooth Muscle Contractility

MEDSCI 305 Laboratory 2: Role of Calcium in smooth muscle contractility
MAKE SURE YOU USE THE CORRECT LAB STREAM - SEE TABS AT BOTTOM OF SCREEN
INSTRUCTIONS - This data has already been edited for you. Your reports should focus on class
(table or figure - decide which is best for your data) and briefly explain it in the d
All data should be dispalyed in grams for your reports. Where relevant variability
If you have any questions or concerns please post to Piazza or email a tutor
Experiment A - Noradrenaline
GROUP # 1 2 3 4 5
Calcium- Calcium- Calcium- Calcium- Calcium-
containing containing containing containing containing
solution solution solution solution solution
Time (min) (g) (g) (g) (g) (g)
1.00 1.07 2.68 1.86 1.62
2.00 1.36 3.24 2.27 2.14
3.00 1.62 3.36 2.53 2.54
4.00 1.77 3.85 2.72 2.86
5.00 1.90 4.07 2.84 3.13
7.50 2.17 4.38 3.01 3.63
10.00 2.34 4.35 3.06 3.86
GROUP # 1 2 3 4 5
Calcium-free Calcium-free Calcium-free Calcium-free Calcium-free
1.00 0.40 0.16 0.14 0.17
2.00 0.52 0.29 0.23 0.27
3.00 0.59 0.46 0.30 0.29
4.00 0.63 0.51 0.26 0.35
5.00 0.66 0.55 0.28 0.41
7.50 0.70 0.54 0.29 0.49
10.00 0.75 0.48 0.29 0.61
Experiment B - KCL (potassium chloride)

GROUP # 19 20 21 22 23
1.00 0.71 1.20 0.70
2.00 0.90 1.21 0.81
3.00 1.03 1.58 0.86
4.00 1.11 1.78 0.87
5.00 1.17 1.97 1.00
7.50 1.33 2.30 1.04
10.00 1.39 2.56 1.06
.
GROUP # 19 20 21 22 23
1.00 0.13 0.51 0.06
2.00 0.25 0.78 0.11
3.00 0.35 0.95 0.12
4.00 0.41 1.06 0.14
5.00 0.47 1.11 0.15
7.50 0.53 1.16 0.10
10.00 0.59 1.11 0.03
BOTTOM OF SCREEN
reports should focus on class data, however you should also include your group data in your results sec
and briefly explain it in the discussion.
rts. Where relevant variability should be displayed and SD is the measure to be used.
o Piazza or email a tutor
6 7 8 9 10 11 12
Calcium- Calcium- Calcium- Calcium- Calcium- Calcium- Calcium-
containing containing containing containing containing containing containing
solution solution solution solution solution solution solution
(g) (g) (g) (g) (g) (g) (g)
0.79 1.53 0.69 0.60 1.65 1.85
1.28 1.81 0.85 0.77 2.25 2.31
1.49 2.06 0.95 0.89 2.69 2.70
1.78 2.28 1.07 0.97 3.00 3.00
1.93 2.45 1.15 1.07 3.29 3.30
2.12 2.73 1.32 1.22 4.02 3.82
2.31 2.87 1.43 1.31 4.26 4.14
6 7 8 9 10 11 12
Calcium-free Calcium-free Calcium-free Calcium-free Calcium-free Calcium-free Calcium-free
(g) (g) (g) (g) (g) (g) (g)
0.30 0.08 0.21 0.07 0.13 0.20
0.37 0.14 0.21 0.10 0.26 0.29
0.42 0.17 0.25 0.12 0.39 0.35
0.58 0.12 0.26 0.12 0.51 0.42
0.58 0.16 0.20 0.14 0.60 0.43
0.62 0.09 0.11 0.08 0.72 0.45
0.65 0.01 0.06 0.00 0.68 0.41
24 25 26 27 28 29 30
(g) (g) (g) (g) (g) (g) (g)
1.36 1.14 3.04 0.84 1.37 2.09 1.38
1.97 1.28 3.38 1.09 1.48 2.51 1.50
2.29 1.44 3.60 1.23 1.57 2.74 1.63
2.59 1.55 3.77 1.35 1.62 2.95 1.72
2.85 1.67 3.96 1.53 0.17 3.10 1.78
3.28 1.86 4.27 1.72 1.70 3.25 1.93
3.54 2.01 4.44 1.93 1.72 3.30 2.02
24 25 26 27 28 29 30
(g) (g) (g) (g) (g) (g) (g)
0.33 0.32 0.27 0.04 0.32 0.50 0.50
0.61 0.48 0.46 0.05 0.55 0.95 0.65
0.82 0.60 0.59 0.00 0.67 1.25 0.70
0.91 0.69 0.60 0.00 0.71 1.48 0.77
0.99 0.76 0.65 0.00 0.72 1.61 0.73
0.90 0.77 0.67 0.00 0.69 1.81 0.69
0.77 0.73 0.59 0.00 0.65 1.82 0.66
in your results section
13 14 15 16 17 18
Calcium- Calcium- Calcium- Calcium- Calcium- Calcium-
containing containing containing containing containing containing
solution solution solution solution solution solution
(g) (g) (g) (g) (g) (g)
0.64 2.18 1.04 1.80
0.71 2.61 1.33 2.10
0.72 2.97 1.45 2.30
0.76 3.13 1.59 2.53
0.82 3.32 1.70 2.63
0.80 3.63 1.88 2.85
0.97 3.80 2.00 3.06
13 14 15 16 17 18
Calcium-free Calcium-free Calcium-free Calcium-free Calcium-free Calcium-free
(g) (g) (g) (g) (g) (g)
0.09 0.18 0.05 0.22
0.06 0.23 0.13 0.37
0.04 0.26 0.16 0.44
0.02 0.31 0.21 0.47
0.00 0.34 0.23 0.47
0.00 0.32 0.23 0.53
0.00 0.34 0.21 0.51
Mean + Ca Standard Dev
Time (min) (g) (g)
0.00
1.00 1.43 0.64
2.00 1.79 0.76
3.00 2.02 0.84
4.00 2.24 0.93
5.00 2.40 0.99
7.50 2.68 1.13
10.00 2.84 1.15
Mean -Ca Standard Dev

(g) (g)
0.17 0.09
0.25 0.12
0.30 0.15
0.34 0.19
0.36 0.20
0.37 0.24
0.36 0.27
Mean +Ca Standard Dev

(g) (g)
1.38 0.71
1.61 0.80
1.80 0.84
1.93 0.90
1.92 1.11
2.27 1.02
2.40 1.06

(g) (g)
0.30 0.18
0.49 0.29
0.61 0.37
0.68 0.43
0.72 0.46
0.73 0.51
0.70 0.52
MAKE SURE YOU USE THE CORRECT LAB STREAM - SEE TABS AT BOTTOM OF SCREEN
INSTRUCTIONS - This data has already been edited for you. Your reports should focus on class dat
(table or figure - decide which is best for your data) and briefly explain it in the disc
All data should be dispalyed in grams for your reports. Where relevant variability sh
GROUP # 1 2 3 4 5 6
Time (min) (g) (g) (g) (g) (g) (g)
1.00 1.07 0.46 1.86 0.64
2.00 1.87 0.46 2.18 0.67
3.00 2.22 0.50 2.48 0.70
4.00 2.30 0.60 2.53 0.73
5.00 2.30 0.58 2.78 0.77
7.50 2.19 0.63 3.07 0.81
10.00 2.56 0.88 3.24 0.81
GROUP # 1 2 3 4 5 6
Time (min) (g) (g) (g) (g) (g) (g)
1.00 0.25 0.30 0.69 -0.02
2.00 0.43 0.37 0.64 -0.05
3.00 0.44 0.48 0.67 -0.07
4.00 0.50 0.48 0.66 -0.10
5.00 0.52 0.44 0.63 -0.12
7.50 0.49 0.53 0.61 -0.19
10.00 0.41 0.42 0.58 -0.22

GROUP # 19 20 21 22 23 24
Time (min) (g) (g) (g) (g) (g) (g)
1.00 1.52 1.01 1.13 0.77 0.44
2.00 1.85 1.14 1.41 1.00 0.58
3.00 2.04 1.24 1.71 1.14 0.67
4.00 2.25 1.32 1.97 1.26 0.74
5.00 2.43 1.41 2.20 1.35 0.74
7.50 2.79 1.56 2.58 1.51 0.72
10.00 3.06 1.67 2.89 1.65 0.50
.
GROUP # 19 20 21 22 23 24
Time (min) (g) (g) (g) (g) (g) (g)
1.00 0.49 0.32 0.16 0.25 0.24
2.00 0.90 0.48 0.46 0.39 0.14
3.00 1.11 0.58 0.54 0.46 0.13
4.00 1.24 0.65 0.63 0.49 0.06
5.00 1.33 0.69 0.73 0.50 0.00
7.50 1.37 0.76 0.87 0.50 0.00
10.00 1.28 0.76 0.87 0.48 0.00
uld focus on class data, however you should also include your group data in your results section
explain it in the discussion.
elevant variability should be displayed and SD is the measure to be used.
mail a tutor
7 8 9 10 11 12 13
(g) (g) (g) (g) (g) (g) (g)
0.33 1.08 - 1.85 2.65 1.27
0.36 1.34 - 1.98 3.02 1.50
0.39 1.54 - 2.06 3.38 1.76
0.41 1.70 - 2.14 3.60 1.96
0.43 1.83 - 2.19 3.75 2.29
0.47 2.03 - 2.27 4.03 2.84
0.49 2.11 - 2.34 4.16 3.12
7 8 9 10 11 12 13
(g) (g) (g) (g) (g) (g) (g)
0.14 0.14 - 0.17 0.45 0.11
0.13 0.22 - 0.23 0.45 0.21
0.11 0.24 - 0.26 0.49 0.22
0.10 0.26 - 0.29 0.49 0.24
0.07 0.27 - 0.30 0.50 0.25
0.00 0.27 - 0.32 0.51 0.31
0.00 0.24 - 0.35 0.50 0.31
25 26 27 28 29 30
(g) (g) (g) (g) (g) (g)
0.60 0.31 1.95 1.02 1.05
0.66 0.39 2.41 1.13 1.28
0.74 0.47 2.71 1.25 1.40
0.80 0.52 3.03 1.33 1.52
0.82 0.54 3.13 1.39 1.56
0.86 0.58 3.56 1.50 1.71
0.93 0.59 3.85 1.59 1.86
25 26 27 28 29 30
(g) (g) (g) (g) (g) (g)
0.37 0.10 0.48 0.20 0.23
0.68 0.16 0.62 0.37 0.38
0.81 0.17 0.90 0.46 0.47
0.88 0.15 0.91 0.52 0.43
0.94 0.14 0.88 0.55 0.46
1.02 0.02 0.84 0.55 0.48
0.90 0.02 0.76 0.52 0.40
ts section
14 15 16 17 18
(g) (g) (g) (g) (g) Time (min)
1.26 1.40 0.77 0.00
1.34 1.73 0.85 1.00
1.45 1.98 0.97 2.00
1.58 2.28 1.05 3.00
1.67 2.49 1.11 4.00
1.83 2.83 1.21 5.00
1.88 2.90 1.19 7.50
10.00
14 15 16 17 18
(g) (g) (g) (g) (g)
0.12 0.15 0.19
0.12 0.17 0.26
0.11 0.18 0.29
0.05 0.17 0.34
0.04 0.13 0.34
0.01 0.01 0.34
0.00 0.00 0.29
Mean + Ca Standard Dev
(g) (g)
1.22 0.66
1.44 0.78
1.62 0.88
1.74 0.93
1.85 0.99
2.02 1.09
2.14 1.13

(g) (g)
0.22 0.19
0.27 0.18
0.29 0.20
0.29 0.22
0.28 0.22
0.27 0.26
0.24 0.24

(g) (g)
0.98 0.49
1.19 0.61
1.34 0.68
1.47 0.76
1.56 0.82
1.74 0.97
1.86 1.10

(g) (g)
0.28 0.13
0.46 0.23
0.56 0.31
0.60 0.35
0.62 0.39
0.64 0.43
0.60 0.40
MAKE SURE YOU USE THE CORRECT LAB STREAM - SEE TABS AT BOTTOM
2.50 OF SCREEN
INSTRUCTIONS - This data has already been edited for you. Your reports should focus on class dat
(table or figure - decide which is best for your data) and briefly explain it in the disc
2.00
All data should be dispalyed in grams for your reports. Where relevant variability sh
1.50
Time (mins)
GROUP # 1 2 3 4 5 6
1.00 Calcium-
Time (min) (g) (g) (g) (g) (g) (g)
1.00 2.05 1.85 1.20 0.93
0.50 1.80
2.00 2.59 2.19 1.30 1.19 1.93
3.00 3.01 2.43 1.50 1.36 2.01
4.00 3.32 2.62 1.70 1.50
0.00
2.10
5.00 3.58 2.79 1.77 0.00
1.61 1.00 2.202.00 3.00
7.50 4.01 2.75 1.93 1.90 2.38

10.00 4.19 2.96 2.00 2.00 2.53
GROUP # 1 2 3 4 5 6
Time (min) (g) (g) (g) (g) (g) (g)
1.00 0.12 3.50 0.20 0.02 0.00
2.00 0.14 0.45 0.29 0.03 0.02
3.00 0.17 0.51
3.00 0.27 0.03 0.01
4.00 0.16 0.53 0.22 0.03 0.00
5.00 0.14 0.55 0.22 0.03 0.00
2.50
7.50 0.12 0.58 0.07 0.02 0.00
10.00 0.02 0.55 0.02 0.00 0.00
Contraction (g)
2.00

1.50
GROUP # 19 20 21 22 23 24
Calcium- Calcium- 1.00 Calcium- Calcium- Calcium- Calcium-
0.50
-
- 1.00 2.00 3.00 4.00 5.00 6.00 7.00
1.00
0.50
Time (min) (g) (g) (g) (g) (g) (g)
1.00 1.30 1.00 0.54 0.43 0.12
2.00 1.50 1.40 - - 0.74
1.00 2.00 3.00 0.87 5.00 0.13
4.00 6.00 7.00
3.00 1.70 1.70 0.85 1.12 0.26
Time (minutes)
4.00 1.80 1.96 0.96 1.33 0.34
5.00 1.80 2.16 1.03 1.47 0.37
7.50 1.90 2.60 1.13 3.54 0.52
10.00 2.00 2.94 1.28 3.54 0.55
.
GROUP # 19 20 21 22 23 24
Time (min) (g) (g) (g) (g) (g) (g)
1.00 0.28 0.86 0.19 0.09 0.25
2.00 0.50 1.36 0.31 0.27 0.11
3.00 0.70 1.66 0.37 0.39 0.13
4.00 0.80 1.85 0.40 0.47 0.05
5.00 0.80 2.00 0.40 0.51 0.05
7.50 0.90 2.12 0.41 0.58 0.01
10.00 1.00 2.07 0.41 0.55 0.00
Time (min) (g)

1.00 0.12
2.00 0.13
3.00 0.26
4.00 0.34
5.00 0.37
7.50 0.52
10.00 0.55
Time (min) (g)

1.00 0.25
2.00 0.11
3.00 0.13
4.00 0.05
5.00 0.05
7.50 0.01
10.00 0.00
1.20
uld focus on class data, however you should also include your group data in your results section
1.00
explain it in the discussion.
elevant variability should be displayed and SD is the measure to be used.
mail a tutor NA 0.80
Time (minutes)
0.60
7 8 9 10 KCl11 12 13
0.40
0.20
(g) (g) (g) (g) (g) (g) (g)
0.73 0.76 1.10 0.41 1.12
0.97 0.87 1.37 0.47 1.51 0.00
1.17 0.93 1.58 0.55 1.70
1.33 0.99 1.70 0.60 1.88 -0.20
1.00 2.00 3.001.46 4.00 5.00 6.00 7.00 8.00 9.00 10.00 0.00 1.00 2.00
1.02 1.84 0.62 2.00
1.70 1.09 (g)
Contraction 2.04 0.71 2.25
1.83 1.14 2.14 0.79 2.34
7 8 9 10 11 12 13
(g) (g) (g) (g) (g) (g) (g)
0.06 0.05 0.04 0.00 0.07
0.08 0.07 0.09 0.00
1.20 0.07
0.12 0.08 0.10 0.00 0.05
0.13 0.06 0.12 0.00
1.00 0.02
0.12 0.07 0.13 0.00 0.01
0.08 0.02 0.13 0.00 0.00
0.80
0.05 0.00 0.13 0.00 0.00
Contraction (g)
0.60
0.40
25 26 27 28 29 30
Calcium- Calcium- Calcium- Calcium- Calcium- 0.20 Calcium-
0.00
5.00 6.00 7.00 8.00 9.00 10.00 -0.20

- 1.00 2.00 3.00 4.00
C
0.20
(g) (g) (g) (g) (g) 0.00 (g)

1.49 1.33 1.05 0.87 0.80
5.00 6.00 7.001.59 8.00 1.41
9.00 10.00 1.12 0.97
-0.20
-
0.73
1.00 2.00 3.00 4.00
minutes)
1.67 1.55 1.21 1.09 0.73
Tim
1.78 1.64 1.31 1.21 0.71
1.88 1.42 1.39 1.33 0.71
2.07 1.92 1.46 1.59 0.61
2.23 1.96 1.60 1.82 0.63
25 26 27 28 29 30
(g) (g) (g) (g) (g) (g)
0.64 0.20 0.00 0.39 0.10
0.73 0.26 0.00 0.43 0.00
0.82 0.26 0.00 0.46 0.00
0.81 0.24 0.00 0.45 0.00
0.80 0.26 0.00 0.44 0.00
0.67 0.16 0.00 0.36 0.00
0.45 0.09 0.00 0.24 0.00
20
ts section
00
80
60 KCl
14 15 16 17 18
40
20
solution solution solution solution NA
solution
1.40 1.44 0.00
00 1.66 1.90 1.00
1.89 2.20 2.00
20 2.13 2.42 3.00
0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00
2.33 2.58 4.00
2.66 3.00Contraction (g) 5.00
2.87 3.00 7.50
10.00
14 15 16 17 18
0.16 0.36 0.00
0.24 0.65 1.00
0.27 0.76 2.00
0.30 0.83 3.00
0.30 0.84 4.00
0.14 0.77 5.00
0.00 0.65 7.50
10.00
2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00

Time (min)
0.00
1.00
2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00
2.00
Time (minutes)
3.00
4.00
5.00
7.50
10.00
-
Standard
Mean + Ca Dev
(g) h (g)
1.23 0.89 0.12 0.50

1.50 1.05 0.13 0.60
1.69 1.19 0.26 0.67
1.86 1.30 0.34 0.74
1.98 1.36 0.37 0.80
2.20 1.73 0.52 0.87
2.32 1.86 0.55 0.90
Standard
Mean -Ca Dev
(g) h (g)
0.10 0.30 0.25 0.11

0.18 0.40 0.11 0.20
0.20 0.48 0.13 0.23
0.20 0.51 0.05 0.25
0.20 0.53 0.05 0.26
0.16 0.52 0.01 0.25
0.12 0.48 - 0.23

(g) (g)
0.89 0.43
1.05 0.45
1.19 0.48
1.30 0.52
1.36 0.54
1.73 0.91
1.86 0.93

(g) (g)
0.30 0.27
0.40 0.41
0.48 0.50
0.51 0.56
0.53 0.60
0.52 0.64
0.48 0.64

Experiment A - Noradrenaline: MEDSCI 305 Laboratory 2: Role of Calcium in Smooth Muscle Contractility

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Experiment A - Noradrenaline: MEDSCI 305 Laboratory 2: Role of Calcium in Smooth Muscle Contractility

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MEDSCI 305 Laboratory 2: Role of Calcium in smooth muscle contractility

Experiment B - KCL (potassium chloride)

Mean -Ca Standard Dev

Mean +Ca Standard Dev

Mean -Ca Standard Dev

Experiment B - KCL (potassium chloride)

Mean -Ca Standard Dev

Mean +Ca Standard Dev

Mean -Ca Standard Dev

7.50 4.01 2.75 1.93 1.90 2.38

Experiment B - KCL (potassium chloride)

Time (min) (g)

Time (min) (g)

5.00 6.00 7.00 8.00 9.00 10.00 -0.20

(g) (g) (g) (g) (g) 0.00 (g)

2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00

1.23 0.89 0.12 0.50

0.10 0.30 0.25 0.11

Mean +Ca Standard Dev

Mean -Ca Standard Dev

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