HOUSE KEEPING &

WASTE MANAGEMENT
DEPARTMENTAL MANUAL
for ISO 15189: 2012 of

SANJEEVI DIAGNOSTICS
(A UNIT OF LOTUS HOSPITALS AND RESEARCH CENTRE LIMITED)

90, Thayumanava Sundram Street, Poondurai Main Road,

Kollampalayam, Erode, Tamil Nadu-638002.

Phone: 04242282828
Mobile: 9843422828
Email: lotusgm@rediffmail.com

Issue No. : 01
Issue Date : 01-06-2021
Copy No. :1
Copy Holder’s Name : NABL

Dr. M. Jayahar Bharathi ., PhD.,

Prepared by
Quality Manager

Dr. K.M. Mohanasoundaram

Reviewed &
M.D., (Micro)
Approved by Technical Director

Dr. E.S. Usha., MBBS., MD

Issued By Lab Director
 HOUSEKEEPING MANUAL
SANJEEVI DIAGNOSTICS RELEASE AUTHORISATION
SJD/ERD/HKM/ A

VERSION 1.0 DATE: 01-06-2021 PAGE 1 OF 1

This Housekeeping & Waste Management MANUAL is issued under the authority
of

Dr. E.S. Usha., MBBS., MD

Lab Director is the property of

SANJEEVI DIAGNOSTICS
(A UNIT OF LOTUS HOSPITALS AND RESEARCH CENTRE LIMITED)

90, Thayumanava Sundram Street, Poondurai Main Road,

Kollampalayam, Erode, Tamil Nadu-638002

Phone: 04242282828
Mobile: 9843422828
Email: lotusgm@rediffmail.com

Name E.S. Usha

Designation LAB DIRECTOR

Signature

Place & Date ERODE 01-06-2021

Issue Date
Issue No : 01 Copy No. 1 Section No. A Page No
01-06-2021

Prepared by QM: Reviewed & Approved by : TD Issued by LD

Amend Date
Amend No : 00
00-00-0000
 HOUSEKEEPING MANUAL
SANJEEVI DIAGNOSTICS AMENDMENT RECORD SHEET
SJD/ERD/HKM/B

VERSION 1.0 DATE: 01-06-2021 PAGE 1 OF 1

Section/ Clause/
Issue Ver. Date of Amd./ Amd. Amd. Approved
Para/ line (as Reasons for Amd.
No. No No made By by
applicable)

1 1 All 01-06-2021 ----- ------ QM TD

Guideline for using Amendment Record sheet:

This Housekeeping Manual belongs to SANJEEVI DIAGNOSTICS and any amendments made to

this manual from time to time is traced through the above format to show the current revisions

made to this manual. When a revision of a section of this manual is issued, the old version is

withdrawn to prevent its inadvertent use

Issue Date
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01-06-2021

Prepared by : QM Reviewed & Approved by TD Issued by LD

Amend Date
Amend No : 00
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 HOUSE KEEPING & WASTE
SANJEEVI DIAGNOSTICS TABLE OF CONTENTS MANAGEMENT
SECTION - C
VERSION 1.0 DATE: 01-06-2021 SJD/ERD/HK/WM/C

Page
Section Contents Version. No No of pages
No
A Release of Authorization
B Amendment Record Sheet 1.0 1 1
C Table of Content 1.0 1 2
D Vocabulary & Abbreviations 1.0 1 3
E List of Record 1.0 1 4
F Authorization Matrix 1.0 2 5
Procedure
Reconstitution of 1 %, 2.5 % & 10 % sodium 1.0 8 7
1
hypochlorite solution
2 Autoclave- Operation 1.0 1 8
3 Culture containers 1.0 2 9
4 Preparation of De-ionized water 1.0 2 11
5 Drinking water analysis 1.0 2 13
6 Overhead tank water analysis 1.0 2 15
7 Personnel protection & safety 1.0 2 17
8 Treatment & disposal of biomedical waste 1.0 1 19
9 Housekeeping procedure 1.0 1 20
1.0
The Bio-Medical Waste Management (Amendment)
10 Rules, 2018

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 HOUSE KEEPING & WASTE
SANJEEVI DIAGNOSTICS VOCABULARY & ABBREVATIONS MANAGEMENT
SECTION -D
Version 1.0 Date: 01-06-2021 SJD/ERD/HK/WM/ D

S.No Abbrevations Expansion

1 NaClO Sodium hypochloride
2 ACF Activated Carbon Filter
3 BA Blood Agar
4 CFU Colony Forming Unit
5 CM Conductivity Meter
6 DM Demineralized
7 HK House Keeping
8 lbs Unit Pressure
9 MA MacConkey Agar
10 MB Mixed Bed
11 No. Number
12 pH Hydrogen Ion Concentration
13 psi Pound per square inch
14 QC Quality control
15 Qty. Quantity
16 QSM Quality System Manual
17 TDS Total Dissolved Salts

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 HOUSE KEEPING & WASTE
SANJEEVI DIAGNOSTICS LIST OF RECORDS MANAGEMENT
SECTION- E
Version 1.0 Date: 01-06-2021 SJD/ERD/HK/WM/E

1 F/SJD/ERD/HKWM/CWR
Cleaning & waste removal log Area-wise 6 Months
L
2 Waste Handovering Register ( to R/SJD/ Housekeeping
6 Months
disposable agency) ERD/HKWM/WHR department
3 Sodium hypo chloride R/SJD/ Housekeeping
6 Months
reconstitution register ERD/HKWM/SHCRR department
4 F/SJD/
Cleaning maintenance log Housekeeping
ERD/HKWM/CML- 6 Months
(Overhead water tank) department
Overhead water tank
5 F/SJD/
ERD/HKWM/CML- Cleaning maintenance log Housekeeping
6 Months
Underground water (Underground water tank) department
tank
6 F/SJD/
Cleaning/ maintenance of RO Housekeeping
ERD/HKWM/CML- RO 6 Months
water tank log department
water tank
7 Weekly cleaning / maintenance
F/SJD/ Housekeeping
log (Air curtain, A/C, fan & 6 Months
ERD/HKWM/WCML department
ventilation)
8 Removal/ segregation log
Housekeeping
F/SJD/ ERD/HKWM/RSL (Decontaminated waste by 6 Months
department
autoclave)
9 F/SJD/ Operation/ maintenance log Microbiology
6 Months
ERD/HKWM/OML (Autoclave) Dept.
10 F/SJD/
Cleaning/ maintenance log Housekeeping
ERD/HKWM/CML- 6 Months
(kitchen) department
kitchen
11 F/SJD/
Cleanliness maintenance log food Housekeeping
ERD/HKWM/CML- food 6 Months
supply area department
supply area
12 Housekeeping
F/SJD/ ERD/HKWM/SD Stock details 6 Months
department
13 Housekeeping
F/SJD/ ERD/HKWM/IF Indent form 6 Months
department

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SANJEEVI DIAGNOSTICS
Version 1.0
1. Monitoring waste segregation
5. Cleaning of functioning areas of the lab
9. Rest room cleaning/ maintenance of
cleanliness
13. Cleaning of A/C machine in all functioning
area and maintaining check list
17. Cleaning of kitchen and maintaining check list
21. Maintenance of coffee machine
25. Cleaning and maintenance of cleanliness in
outside of laboratory functioning room
29. Removal and segregation of decontaminated
waste by autoclave
33. Cleaning of records, register, file, text book &
other recording materials
37. Receiving/ acceptance of delivered items
41. Cleaning/ maintenance of RO Water tank
Applicable
Issue No : 01
Amend No : 00
HOUSE KEEPING &
RESPONSIBILITY & AUTHORISATION MATRIX WASTE MANAGEMENT DEPARTMENT
SECTION F
Date: 01-06-2021 SJD/ERD/HK/WM/F
3. Handovering of segregated waste to waste 4. Maintaining of register for waste
2. Removal of segregated waste
disposable agency disposable agency
8. Preparation/ arrangement of 2.5%
7. Maintaining check list for waste removal/
6. Monitoring the cleanliness of functioning area sodium hypo chloride solution container in
cleaning of functioning area
all departments
11. Reconstitution of sodium hypo chloride solution,
10. Removal of waste from rest room and 12. Maintaining check list for reconstitution
maintenance check list 10%, 2.5% & 1% of sodium hypo chloride solution
15. Cleaning/ maintenance of overhead water tank/
16. Monitoring maintenance of water
14.Cleaning of fun/ ventilation/ maintaining check list
maintaining check list supplies & maintaining check list
18. Monitoring food preparation and supply of food 20. Monitoring and maintenance of coffee,
19. Arrangement of morning Tiffin for patients
to staff tea supplies
22. Monitoring segregation of waste generated in 23. Cleaning and maintenance of cleanliness of MD
24. Making arrangements for meeting held in
canteen & coffee supply area room, all HOD cabins, conference hall and other
conference hall
office rooms
26.Removal and segregation of parcel and courier 27. Segregation and maintenance of sample 28. Monitoring the maintenance of autoclave
waste transporting box and its operations
32. Cleaning of dust and maintenance of
30. Washing and cleaning of laboratory staff coats 31. Washing and cleaning of mat
cleanliness in all functional area of laboratory
36. Maintenance of stock & consumables/
34. Cleaning of curtain & screen 35. Preparation of action request
preparation of indent
38. Arrangement of disposable shoe cover in all
39. Maintenance of document and check list 40. shoe machine cleaning & maintenance
functional area
42. Cleaning/ maintenance of water tank
43. Overall supervising
underground
X Not Applicable
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 HOUSE KEEPING &
SANJEEVI DIAGNOSTICS RESPONSIBILITY & AUTHORISATION MATRIX WASTE MANAGEMENT DEPARTMENT
SECTION F
Version 1.0 Date: 01-06-2021 SJD/ERD/HK/WM/F

1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 4 4 4 4
Employee Name 1 2 3 4 5 6 7 8 9
0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3

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 HOUSE KEEPING & WASTE
RECONSTITUTION OF 1 %, 2.5 % & 10 %
SANJEEVI DIAGNOSTICS MANAGEMENT
SODIUM HYPOCHLORITE SOLUTION
PROCEDURE – 01
VERSION 1.0 DATE: 01-06-2021 SJD/ERD/HK/WM/P/01

1. Principle:
To check the germicidal quality of the hypochlorite solution
2. Consumables:
 4% Sodium Hypochlorite solution (Household Bleach)
 Tap water
 Starch iodide paper
3. Procedure:
3.1 Perform this procedure whenever the required % Hypochlorite solution is prepared
(or surprise checking)
3.2 Follow the table below for the preparation
Household Bleach
Required Final
4% sodium Water Purpose
Percentage Volume
hypochlorite

1% 1 ml 99 ml 100 ml Work table cleaning

2.5 % 2.5 ml 97.5 ml 100 ml Discarding tips

10% 10 ml 90 ml 100 ml Blood / Body fluid Spillage

3.3 Check the quality of the prepared solution with a starch iodide paper
3.4 Get the approval from the Microbiology department
4. Observation and Interpretation:
Development of purple color on the starch iodide paper indicates that the prepared % solution of
sodium hypochlorite is of germicidal quality
5. Record: MBL/HK/IQR
6. Reference: Mackie & Mc Cartney, Practical Medical Microbiology – 13th edition – Page no: 301

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 HOUSE KEEPING & WASTE
AUTOCLAVE- OPERATION &
SANJEEVI DIAGNOSTICS MANAGEMENT
DECONTAMINATION PROCEDURE
PROCEDURE - 02
Version 1.0 Date: 01-06-2021 SJD/ERD/HK/WM/P/02

1. Principle:
To check whether the autoclave is able to maintain a temperature of 121 o C at 15 lbs pressure during
operation (1 HOUR)

2. Consumables:
 Biological Indicator
 Chemical Indicator
 Container to be decontaminated

3. Procedure:
3.1 Perform this procedure for every day, whenever the autoclave is operated
3.2 Load the autoclave with the bags (red & yellow) to be decontaminated/ sterilization
3.3 Stick Chemical Indicator label to any one of the bags to be decontaminated
3.4 Switch on the autoclave and decontaminate the materials for 1 hour at 121o C at 15 lbs
3.5 De-load the containers from the autoclave after the steam is let out of the autoclave when
the pressure shows “0”
3.6 Observe the autoclave label for any colour change
3.7 Get the approval from the Microbiology department

4. Observation and Interpretation:

Change of light WHITE portion of Chemical Indicator to dark grey/ black indicates effective autoclaving
(decontamination) process

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 HOUSE KEEPING & WASTE
AUTOCLAVE- OPERATION &
SANJEEVI DIAGNOSTICS MANAGEMENT
DECONTAMINATION PROCEDURE
PROCEDURE - 02
Version 1.0 Date: 01-06-2021 SJD/ERD/HK/WM/P/02

Yellow (Non Red Black Translucent white

chlorinated plastic (Non box (Puncture,
bags, autoclave chlorinated leak, tamper
safe plastic bags or proof)
plastic bags or containers)
containers)
Swab sticks from VTM Used VTM tubes Not to be Needles
Used Cotton swabs, PCR Tubes, Wells used for the Discarded &
alcohol wipes, tissue time being contaminated metal
papers sharps
PPE Items including Plate sealers
masks, gowns, shoe
covers, head covers
Trashed papers Vacutainers, Used
Microcentrifuge tubes,
tips
Any non plastic item Gloves
that is soiled with
sample
Biodegradable Packaging material
General waste removed from triple
layered packed samples
Non biodegradable
general waste

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 HOUSE KEEPING & WASTE
SANJEEVI DIAGNOSTICS CULTURE CONTAINERS MANAGEMENT
PROCEDURE - 03
VERSION 1.0 DATE: 01-06-2021 SJD/ERD/HK/WM/P/03

1. Principle:
To prepare and check if the culture containers prepared in-house are sterile
2. Consumables:
 Culture containers
 Autoclave bin
 Culture media – Blood Agar (BA)
 Autoclave
 Incubator maintained at 37 o C
3. Procedure:
3.1 Perform this procedure once in 10 – 15 days
3.2 Wipe the containers with gauze cloth
3.3 Fill the autoclave bin with the containers
3.4 Hand over the bin for autoclaving to Microbiology department
3.5 After autoclaving, the Microbiology department checks the sterility of the culture containers
3.6 To check the sterility of the culture containers, pick up any one of the autoclaved culture
containers randomly and swab the container
3.7 Streak for isolation using a sterile loop and inoculate the swab on a plate on a Blood Agar
3.8 Incubate the plates overnight at 37 o C
4. Observation & Interpretation:
4.1 On observation, if the culture plate shows no growth, the prepared culture containers are
sterile and the autoclave is also working properly
4.2 If there is any growth the culture container are not sterilized properly and hence not
suitable for use
4.3 The autoclaving procedure and sterility are recorded in the autoclave register of
Microbiology department
4.4 The sterile containers are handed over to housekeeping department for issue
4.5 After receiving the sterile containers, put a label with the date of sterilization and send the
` containers to the Collection center

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 HOUSE KEEPING & WASTE
PREPARATION OF DE-IONIZED
SANJEEVI DIAGNOSTICS MANAGEMENT
WATER
PROCEDURE 04
VERSION 1.0 DATE: 01-06-2021 SJD/ERD/HK/WM/04

1. Principle:
To check if the deionized water prepared is of Reagent Grade II
2. Consumables:
 Sterile container
 Fresh deionized water
 MacConkey Broth
 Blood agar (BA)
 MacConkey agar (MA)
 Streaking accessories
 Universal pH indicator
 Bacteriological Incubator

3. Instrument:
 Biosafety Hood
 Conductivity meter
 Resistivity meter

4. Procedure:
4.1 Perform this procedure whenever deionized water is prepared
4.2 Collect the fresh deionized water in sterile plastic containers and send it to the microbiology
laboratory to test for colony counts (when RO unit is changed)
4.3 Inoculate the water sample onto MacConkey broth
4.4 Incubate the inoculated broth tubes at 37oC
4.5 Observe the tubes for any growth on the following day (after 24 hours)
4.6 If no growth, continue to incubate the tubes for another 24 hours
4.7 If growth occurs subculture on BA/MA and incubate for 24 hours

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 HOUSE KEEPING & WASTE
PREPARATION OF DE-IONIZED
SANJEEVI DIAGNOSTICS MANAGEMENT
WATER
PROCEDURE 04
VERSION 1.0 DATE: 01-06-2021 SJD/ERD/HK/WM/04

4.8 Record the observations on the following day (after 24 hours) and record the report in
MBL/HK/IQR
4.9 Check the pH of the deionized water using the universal pH indicator
4.10 Check the conductivity and resistivity of the deionized water. And record the report in
MBL/HK/IQR
4.11 Send the water sample to the Biochemistry department to check the sodium & chloride
content of the deionized water and get the approval from the Biochemistry department. Record the
report

5. Observation and Interpretation:

5.1 Colony counts:
 If there is no growth or < 10 colony forming units (CFU) /ml, the water is considered as
Reagent Grade II water to be used for lab purposes
 If there is growth of > 10 CFU/ml, then the whole batch of water is discarded
5.2 pH:
 Check the pH using universal indicator
 Confirm that the pH of Deionized water is between 6.8 & 7.2
5.3 Conductivity:
 Check if the conductivity and resistivity of water is < 1.0 respectively
5.4 Chloride content:
 Sodium and Chloride test is processed as per Clinical biochemistry Procedure
 Reagent grade water II used for laboratory purposes should not contain any chloride
6. Record: MBL/HKIQR
6.1 Reference: www.apswater.com/page No51

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 HOUSE KEEPING & WASTE
SANJEEVI DIAGNOSTICS DRIKING WATER ANALYSIS MANAGEMENT
PROCEDURE 05
VERSION 1.0 DATE: 01-06-2021 SJD/ERD/HK/WM/P/5

1. Principle:
To check if the drinking water is free from any bacteria by looking for the colony counts.

2. Consumables:
 Freshly collected drinking water
 Sterile syringe (10 ml) & needle.
 Blood Agar (BA) plates
 MacConkey Agar (MA) plates
 MacConkey Broth
 Streaking accessories

3. Instrument:
 Bacteriological incubator
 Biosafety hood

4. Procedure:
4.1 Perform this procedure once a month.
4.2 Collect sample of drinking water from a freshly opened can in a sterile container (100 ml)
send it to the microbiology department for testing the colony counts.
4.3 Inoculate the water sample on MacConkey broth.
4.4 Incubate the inoculated broth at 37o C.
4.5 Observe the broth bottles for any growth the following day (after 24 hours).
4.6 If no growth, continue to incubate the broth bottles for another 24 hours.
4.7 If there is any growth, subculture on BA/MA and incubate overnight
4.8 Record the observations the following day (after24 hours).

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 HOUSE KEEPING & WASTE
SANJEEVI DIAGNOSTICS DRIKING WATER ANALYSIS MANAGEMENT
PROCEDURE 05
VERSION 1.0 DATE: 01-06-2021 SJD/ERD/HK/WM/P/5

5. Observation and Interpretation:

5.1 Absence of acid and gas at the end of 48 hours indicates a negative test. No colour change of the
media and no turbidity, and the water is suitable for drinking purpose.
5.2 Presence of acid and gas is presumptive evidence of the presence of coli forms; the water is
unsuitable for drinking purpose.

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 HOUSE KEEPING & WASTE
OVERHEAD TANK WATER
SANJEEVI DIAGNOSTICS MANAGEMENT
ANALYSIS
PROCEDURE 06
VERSION 1.0 DATE: 01-06-2021 SJD/ERD/HK/WM/p/6

1. Principle:
To check if the water collected from over head tanks and sump is free from any bacteria by looking for
the colony counts.

2. Consumables:
 Freshly collected Tap water
 Sterile container
 MacConkey Broth
 Blood Agar (BA) plates
 MacConkey Agar (MA) plates
 Streaking accessories

3. Instrument:
 Bacteriological incubator
 Biosafety hood

4. Procedure:
4.1 Perform this procedure once a month; cleaning of tank is two months once.
4.2 Collect the fresh tap water in sterile plastic containers and send it to the microbiology
laboratory to test for colony counts (tap water)
4.3 Inoculate the water sample onto Mac Conkey Broth.
4.4 Incubate the inoculated broth tubes at 37oC.
4.5 Observe the tubes for any growth the following day (after 24 hours).
4.6 If no growth, continue to incubate the tubes for another 24 hours.
4.7 If growth occurs subculture on BA / MA and incubate for 24 hours.

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 HOUSE KEEPING & WASTE
OVERHEAD TANK WATER
SANJEEVI DIAGNOSTICS MANAGEMENT
ANALYSIS
PROCEDURE 06
VERSION 1.0 DATE: 01-06-2021 SJD/ERD/HK/WM/p/6

4.8 Record the observations on the following day (after 24 hours) and record the report in
MBL/HK/IQR
4.9 Send the water sample to the Biochemistry department to check the sodium & chloride
content of the tap water and get the approval from the Biochemistry department. record the report in
MBL/HK/IQR

5. Observation and Interpretation:

If there is growth > 10 colony forming units (CFU) /100 ml, the water is used only for washing purpose
and not for drinking.

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