Shimadzu HPLC Systems Created on: June 07, 2002 – Ver.

Shimadzu HPLC System Customer Requirements and

Proposals from Shimadzu
[A reference for producing DQ (Design Qualification) documents]

Shimadzu Corporation
Analytical & Measuring Instruments Division

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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A

Contents

1. Summary....................................................................................................................................... 3

2. Process Flow -- From Design Qualification to Vendor Selection ................................................... 4

3. Objectives (An Example)............................................................................................................... 5

4. List of Customer Requirements..................................................................................................... 6

5. The Vendor Proposal and Evaluating its Content ........................................................................ 11

6. Vender Selection Criteria (Suggested as Reference).................................................................. 17

7. Reference Documents................................................................................................................. 18

7.1 Standard LC Specifications ............................................................................................. 18

(1) Pump....................................................................................................................... 18

(2) Auto-Sampler........................................................................................................... 19

(3) Column Oven .......................................................................................................... 21

(4) UV-VIS Detector ...................................................................................................... 22

7.2 Standard LC Workstation and PC System Specifications ............................................... 22

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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A

1. Summary

This document, “Shimadzu HPLC System Customer Requirements and Proposals from Shimadzu”, was created to support customers with the DQ (Design
Qualification) process.

DQ is the first step a customer takes before purchasing a system. The DQ process is a necessary step for verifying the suitability of the equipment selection, by
listing all the requirements and criteria with respect to the total system, based on the objectives for acquiring the system. If inappropriate requirements or criteria
are designated during DQ it could have a major effect on the effectiveness or economics of the equipment after installation. Therefore, it is necessary to thoroughly
verify the requirements. Shimadzu will help customers prepare the DQ documentation by providing documents such as this one and IQ/OQ information for each
analytical device.

In general, the procedure for preparing DQ documentation is as follows.

1. Clarify the objectives for using the equipment.
2. Clarify the functional and performance requirements.
3. Clarify the vendor selection criteria.
4. Determine the content and frequency of validations.
5. Evaluate the vendor proposal based on documents provided by the vendor.
6. If any items do not meet the requirements, evaluate the risk.
7. Decide which vendor and hardware will be selected.
Thus, it is of utmost importance to clarify the objectives for operating the system and to carefully document the process.
Next, a flowchart of the DQ process is shown.

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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A

2. Process Flow -- From Design Qualification to Vendor Selection

・ It has become necessary to comply

with certain laws or regulations.
User (Customer) ・ Certain tests will be conducted. Vendor (Shimadzu Corporation)
・ A certain system is now necessary.
・ It has become necessary to
purchase certain equipment.

Stipulate Requirements

Prepare DQ (Design Qualification）

(DQ consists mainly of the following.)

1. Clarify acquisition objectives A proposal is prepared based on the

2. Clarify system requirements content of the DQ documentation. This
includes items such as:
3. Clarify IQ/OQ requirements
Submit Requirements 1. System Proposal
to Vendor
2. Equipment Proposal
3. IQ/OQ Documents
Evaluate DQ requirements and the 4. Estimated Quotation Price
vendor’s proposal. Proposal Submitted

Select vendor
Confirmation of evaluation contents
Sign Contract

IQ/OQ document preparation

Confirm IQ/OQ documents
Proposal of IQ/OQ

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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A

3. Objectives (An Example)

An HPLC system will be acquired to control the quality of substance yyyyy contained in product xxxxx. The control parameters consist of the following.

(1) Product Name:

(2) Analyzed Substance:
(3) Inspection Items / Standards

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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A

4. List of Customer Requirements

A list is made of all system requirements. Place a check mark in the checkbox corresponding to items that must be included in the system.

Requirements for each HPLC Module

Module Requirements Select User Comments
1 Pump Isocratic system □
Low pressure gradient system □
High pressure gradient system □
Gradient mixer □
On-line degasser □
2 Detector UV-VIS detector □
3 Oven Column oven □
4 Injector Manual injector □
Auto-sampler □
Sample cooler □
5 Data Workstation for the LC
Processing □
6 □
7 □
8 □
9 □
10 □

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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A

Functional Requirements for the HPLC system

Functional Requirements Select User Comments
1 Noise must be ≤ 1.0ｘ10-5 AU □
-4
2 Drift must be ≤ 6.0ｘ10 AU/h □
3 RSD for retention time reproducibility must be < 0.5 % □
4 RSD for peak area reproducibility must be < 1.0 % □
5 Gradient accuracy must be ≤ ±1.0 % □
6 □
7 □
8 □
9 □
10 □

Requirements regarding the vendor and the support they will provide
Requirements Select User Comments
1 Must have ISO 9001 or equivalent quality standards
certification □
2 Product development records must be accessible □
3 Products must include a validation declaration or equivalent
documentation □
4 Must have a revision management system that notifies users
when products are upgraded □
5 Must provide support via telephone □
6 Must provide support via email □
7 Must provide support via the Internet □
8 Must sponsor seminars, etc. □
9 Operator training support must be available □
10 IQ/OQ support must be available □
11 □
12 □

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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A

IQ (Installation Qualification) Inspection Requirements

Inspection Requirements Select User Comments
1 Must check equipment list □
2 Must check product specifications □
3 Must confirm Purchase Order Compliance Certificate □
4 Must check that all instruction manuals are included □
Must check all certification documents (“Purchase Order Compliance Certificate”,
5 □
“Inspection Evaluation Report”, etc.)
6 Must check installation environment (indoor environment, power supply, etc.) □
7 Must check equipment information and accessories □
8 Must certify for basic operation and maintenance skills training □
9 □
10 □

OQ (Operational Qualification) Inspection Requirements

Inspection Requirements Select User Comments
1 Pump Must perform ROM and RAM self-diagnostics □
Must check firmware version □
Must test display and LEDs □
Must test the time program □
Must perform pulsation test □
Must test pressure limiter □
Must check flow rate control parameters □
Must test flow rate accuracy □
Must test leak sensor □
2 Degasser Must check vacuum pump □
Must test leak sensor □
3 UV Detector Must perform ROM and RAM self-diagnostics □

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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A
Must check firmware version □
Must test initialization □
Must test the time program □
Must test lamp energy □
Must test wave length accuracy □
Must test light source illumination time □
Must check indicated light absorption and voltage output □
Must test leak sensor □
4 Oven Must perform ROM and RAM self-diagnostics □
Must check firmware version □
Must test display and LEDs □
Must test the time program □
Must test accuracy of temperature settings (room temp.-5 ºC) □
Must test accuracy of temperature settings (40/50 ºC) □
Must check precision of temperature adjustments (room temp.-5 ºC) □
Must check precision of temperature adjustments (40/50 ºC) □
Must check functionality of leak sensor □
6 System Controller Must perform ROM and RAM self-diagnostics □
Must check firmware version □
Must adjust display screen contrast □
Must check each display screen □
Must check system control □
Must check the backup memory system □
7 Auto-Sampler Must perform ROM and RAM self-diagnostics □
Must check firmware version □
Must test display and LEDs □
Must check basic functionality □
Must test accuracy of injection volume □
(Sample Cooler) Must test accuracy of temperature □

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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A
8 Workstation Must check the computer memory □
Must check date and time □
Must check the security system □
Must test the hard disk drive □
Must check the printer driver □
Must check the software version □
Must perform program alteration check □
Must check user access control system □
Functionality must be checked □
9 System Must test the concentration gradient □
Must test noise and drift levels □
Must test UV-VIS reproducibility □

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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A

5. The Vendor Proposal and Evaluating its Content

The proposal from the vendor is evaluated to determine whether it satisfies the customer requirements. For each item, place a check mark in the checkbox if
the requirement has been satisfied.

Requirements for each HPLC Module

Module Corresponding Proposal from Evaluation Comments
Requirements Satisfied
Shimadzu
1 Pump Isocratic system Possible with LC-10ADvp or
LC-10ATvp □
Low pressure gradient system Possible by combining LC-10ADvp
or LC-10ATvp with FCV-10ALvp □
High pressure gradient system Possible by using 2 or 3
LC-10ADvp or LC-10ATvp units □
Gradient mixer Able to satisfy the requirement □
On-line degasser Possible with DGU-13A or
DGU-14A units □
2 Detector UV-VIS detector Possible with SPD-10Avp or
SPD-10AVvp detector □
3 Oven Column oven Possible with CTO-10Avp,
CTO-10ACvp or CTO-10ASvp □
4 Injector Manual injector Possible with Rheodyne 7725 or
7725i □
Auto-sampler Possible with SIL-10ADvp or
SIL-10AHTA/C □
Sample cooler A sample cooler is optional for
SIL-10ADvp and is standard □
equipment on SIL-HTC.
5 Data Workstation for the LC Can satisfy requirement with
Processing CLASS-VP □
6 □
7 □
8 □
9 □
10 □

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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A

Functional Requirements for the HPLC system

Functional Requirements Corresponding Proposal from Shimadzu Satisfied Evaluation Comments
1 -5 Able to satisfy the requirement
Noise must be ≤ 1.0ｘ10 AU □
2 -4 Able to satisfy the requirement
Drift must be ≤ 6.0ｘ10 AU/h □
3 RSD for retention time reproducibility must be < Able to satisfy the requirement □
0.5 %
4 RSD for peak area reproducibility must be < Able to satisfy the requirement □
1.0 %
5 Gradient accuracy must be ≤ ±1.0 % Able to satisfy the requirement □
6 □
7 □
8 □
9 □
10 □

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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A

Requirements regarding vendor support

Requirements Corresponding Proposal from Shimadzu Satisfied Evaluation Comments
1 Must have ISO 9001 or equivalent quality Shimadzu is ISO 9001 certified.
standards certification □
2 Product development records must be accessible Vendor can be inspected □
3 Products must include a validation declaration or Able to satisfy the requirement
equivalent documentation □
4 Must have a revision management system that Provides prompt notification via CLASSmate and
notifies users when products are upgraded the Internet. □
5 Must provide support via telephone Able to satisfy the requirement □
6 Must provide support via email Able to satisfy the requirement □
7 Must provide support via the Internet Able to satisfy the requirement □
8 Must sponsor seminars, etc. Able to satisfy the requirement. Seminars, etc.,
held whenever appropriate. □
9 Operator training support must be available Able to satisfy the requirement. On- and off-site
training is offered. □
10 IQ/OQ support must be available Able to satisfy the requirement. IQ/OQ
inspections are performed by certified engineers. □
11 □
12 □

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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A

IQ (Installation Qualification) Inspection Requirements

Inspection Requirements Corresponding Proposal from Evaluation Comments
Satisfied
Shimadzu
1 Must check equipment list Able to satisfy the requirement □
2 Must check product specifications Able to satisfy the requirement □
3 Must confirm Purchase Order Compliance Certificate Able to satisfy the requirement □
4 Must check that all instruction manuals are included Able to satisfy the requirement □
Must check all certification documents (“Purchase Order Compliance Able to satisfy the requirement
5 □
Certificate”, “Inspection Evaluation Report”, etc.)
Must check installation environment (indoor environment, power Able to satisfy the requirement
6 □
supply, etc.)
7 Must check equipment information and accessories Able to satisfy the requirement □
8 Must certify for basic operation and maintenance skills training Able to satisfy the requirement □
9 □
10 □
Shimadzu Corporation will provide IQ documentation for the proposed equipment.

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Shimadzu HPLC Systems
OQ (Operational Qualification) Inspection Requirements
1 Pump
(Degasser)
2 UV-VIS Detector
3 Oven
Created on: June 07, 2002 – Ver. A
Inspection Requirements Corresponding Proposal from Evaluation Comments
Satisfied
Shimadzu
Must perform ROM and RAM self-diagnostics Able to satisfy the requirement □
Must check firmware version Able to satisfy the requirement □
Must test display and LEDs Able to satisfy the requirement □
Must test the time program Able to satisfy the requirement □
Must perform pulsation test Able to satisfy the requirement □
Must test pressure limiter Able to satisfy the requirement □
Must check flow rate control parameters Able to satisfy the requirement □
Must test flow rate accuracy Able to satisfy the requirement □
Must test leak sensor Able to satisfy the requirement □
Must check vacuum pump Able to satisfy the requirement □
Must test leak sensor Able to satisfy the requirement □
Must perform ROM and RAM self-diagnostics Able to satisfy the requirement □
Must check firmware version Able to satisfy the requirement □
Must test initialization Able to satisfy the requirement □
Must test the time program Able to satisfy the requirement □
Must test lamp energy Able to satisfy the requirement □
Must test wave length accuracy Able to satisfy the requirement □
Must test light source illumination time Able to satisfy the requirement □
Must check indicated light absorption and Able to satisfy the requirement
voltage output □
Must test leak sensor Able to satisfy the requirement □
Must perform ROM and RAM self-diagnostics Able to satisfy the requirement □
Must check firmware version Able to satisfy the requirement □
Must test display and LEDs Able to satisfy the requirement □
Must test the time program Able to satisfy the requirement □
Must test accuracy of temperature settings Able to satisfy the requirement
(room temp. - 5 ºC) □
Must test accuracy of temperature settings Able to satisfy the requirement
(40/50 ºC) □
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Shimadzu HPLC Systems
4 Auto-Sampler
(Sample Cooler)
5 System Controller
6 Workstation
7 HPLC System
Shimadzu Corporation will provide OQ documentation for the proposed equipment.
Created on: June 07, 2002 – Ver. A
Must check precision of temperature Able to satisfy the requirement
adjustments (room temp. - 5 ºC) □
Must check precision of temperature Able to satisfy the requirement
adjustments (40/50 ºC) □
Must check functionality of leak sensor Able to satisfy the requirement □
Must perform ROM and RAM self-diagnostics Able to satisfy the requirement □
Must check firmware version Able to satisfy the requirement □
Must test display and LEDs Able to satisfy the requirement □
Must check basic functionality Able to satisfy the requirement □
Must test accuracy of injection volume Able to satisfy the requirement □
Must test accuracy of temperature Able to satisfy the requirement □
Must perform ROM and RAM self-diagnostics Able to satisfy the requirement □
Must check firmware version Able to satisfy the requirement □
Must adjust display screen contrast Able to satisfy the requirement □
Must check each display screen Able to satisfy the requirement □
Must check system control Able to satisfy the requirement □
Must check the backup memory system Able to satisfy the requirement □
Must check the computer memory Able to satisfy the requirement □
Must check date and time Able to satisfy the requirement □
Must check the security system Able to satisfy the requirement □
Must test the hard disk drive Able to satisfy the requirement □
Must check the printer driver Able to satisfy the requirement □
Must check the software version Able to satisfy the requirement □
Must perform program alteration check Able to satisfy the requirement □
Must check user access control system Able to satisfy the requirement □
Functionality must be checked Able to satisfy the requirement □
Must test the concentration gradient Able to satisfy the requirement □
Must test noise and drift levels Able to satisfy the requirement □
Must test UV-VIS reproducibility Able to satisfy the requirement □
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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A

6. Vender Selection Criteria (Suggested as Reference)

The manufacturer will be selected based especially on the following evaluation criteria.
・ The manufacturer can satisfy all analytical system requirements
・ The manufacturer has a thorough quality management system with respect to product development.
・ The manufacturer is able to fully support the system after installation.

Shimadzu Corporation’s “Shimadzu HPLC System” fully satisfies all these requirements, so Shimadzu has been selected. The table below lists the criteria for
selecting the vendor.

List of Vendor Selection Criteria

Criteria Explanation
1 System Development ・ Vendor is ISO 9001 certified and system development is carried out according to
Capability development standards.
2 System Evaluation ・ Vendor is ISO 9001 certified and system evaluation is carried out according to
Capability evaluation standards.
3 Vendor Inspections ・ Vendor will accommodate inspections
4 Validations ・ Vendor provides support for validation procedures, such as DQ/IQ/OQ
5 Installation ・ Installation is performed by qualified service providers that have passed a
certification program.
6 Support ・ Support via telephone is available.
・ Support via email is available.
・ The latest information is provided via the Web.
・ The latest information is continually being provided by sponsoring seminars, etc.
・ Support in the form of training is available

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Shimadzu HPLC Systems
7. Reference Documents
7.1 Standard LC Specifications
Standard specifications for an HPLC system manufactured by Shimadzu Corporation
(1) Pump
Product Name
Pump Method
Flow Rate Setting Range (in
constant flow rate mode)
Flow Rate Accuracy
(in constant flow rate mode)
Flow Rate Precision
(in constant flow rate mode)
Pressure Limiters
Time Program
Accuracy of Indicated Pressure
Size and Weight
Operating Temperature Range
Power Supply Requirements
(1-1) High Pressure Gradient Specifications
Number of liquids in solvent mixtures
Gradient Profiles
Time Program Steps
Program Time Settings
Mixing Ratio Setting Range
Accuracy of Concentration Settings
(1-2) Low Pressure Gradient Specifications
Number of liquids in solvent mixtures
Gradient Profiles
Time Program Steps
Program Time Settings
Mixing Ratio Setting Range
Accuracy of Concentration Settings
Created on: June 07, 2002 – Ver. A
LC-10ADvp / LC-10ATvp
Micro-volume double plunger (LC-10ADvp) and serial double plunger (LC-10ATvp)
0.001 - 5.000 mL/min [1.0 - 39.2 MPa]
5.001 - 9.999 mL/min [1.0 - 19.6 MPa]
Within ±2% or ±2 µL/min, whichever is greater.
[Given 0.01 - 5 mL/min (for LC-10ADvp) or 0.1 - 5 mL/min (for LC-10ATvp), and 1.0 - 39.2 MPa, using water and with a constant room
temp of 20 ºC. Except during low pressure gradient liquid delivery mode.]
Within ±0.3% (RSD < 0.1 %)
(Given 0.1 - 5 mL/min, 1.0 - 39.2 MPa, water, constant room temp of 20 °C.)
High pressure and low pressure
Flow rate, pressure, events, LOOP (program iteration), 10 files. A total of 320 steps.
Within ±2% or ±0.5 MPa, whichever is greater.
W260 x H140 x D420 mm, 11 kg
4 - 35 °C
AC 100 V, 100 VA, 50/60 Hz
When controlled by a liquid delivery unit When controlled by a SCL-10Avp unit
Two liquids Two or three liquids
Step and linear, multi-step Step, linear and exponential, multi-step
10 Programs, 320 steps total 20 Programs, 400 steps total
0.01 - 999.99 min, 0.01 min steps 0.01 - 9999.9 min, 0.01 min steps
0 - 100% (0.1 % steps)
±1%
(Given a water/acetone mixture at 0 - 100%, 0.1 - 3 mL/min and 1.0 - 39.2 MPa)
When controlled by a liquid delivery unit When controlled by a SCL-10Avp unit
Four liquids max. Four liquids max.
Step and linear, multi-step Step, linear and exponential, multi-step
10 Programs, 320 steps total 20 Programs, 400 steps total
0.01 - 999.99 min, 0.01 min steps 0.01 - 9999.9 min, 0.01 min steps
0 - 100% (0.1 % steps)
±1% (Given a two-liquid gradient of water/acetone, 1 mL/min flow and 10.0 MPa pressure or less.)
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Shimadzu HPLC Systems
(2) Auto-Sampler
(2-1) Model SIL-10ADvp
Product Name
Method
Injection Volume Setting Range
Number of Samples Processed
Injection Volume Reproducibility
Number of Injection Iterations
Analysis Time Settings
No. of Steps for Injection Conditions
Sample Suction Rate
Operating Pressure
Operating pH range
Liquid Contact Surfaces
Number of Pretreatment Files
Reagent Vials
Reagent Mixing
Reaction Time Settings
Size and Weight
Ambient Operating Temp Range
Power Supply Requirements
(Sample Cooler)
System Method
Temperature Setting Range
Temperature Setting Accuracy
Size and Weight
Ambient Operating Temp Range
Power Supply Requirements
Created on: June 07, 2002 – Ver. A
SIL-10ADvp
Total sample volume injection and variable sample volume injection (zero sample loss during injection)
0.1 - 50 µL (standard)
1 - 500 µL (with optional 500 µL sample loop [228-33355-01])
150 (using 1 mL vials), 768 (using micro plates)
RSD < 0.3% (when injecting 10 µL, depending on conditions)
1 - 30 per sample
0.01 - 9999.9 min. (0.01 min. steps)
102 steps maximum
0.1 - 15 µL/sec (0.1 µL/sec steps for 1 µL/sec or less, 1 µL/sec steps for over 1 µL/sec)
34.3 MPa (350 kgf/cm2) maximum
pH 1 - 13
(If using a liquid of pH 10 or higher, the gasket for the 6-port valve rotor and stator, and the needle seal must be replaced by the Delrin
gasket kit [228-21708-92] and PEEK needle seal [228-33355-01] respectively. In this case, the 6-port valve max pressure will be 19.6
MPa)
Stainless steel (SUS 316), ceramics, Vespel, Teflon, etc.
20
Three 10 mL capacity vials can be installed. (using racks 6, 7, or 16)
Possible
Possible (0.1 - 120.0 min (0.1 min steps)
W260 x H280 x D420 mm, 19 kg
4 - 35 °C
AC 100 V, 300 VA, 50/60 Hz
Aluminum block is cooled via Peltier elements
4 - (room temp - 5) °C
(Can be controlled to 4 °C, given a 30 °C or less room temp and 60% or less humidity)
±3 ºC
Controller Section: W260 x H70 x D420 mm, 6.5 kg
Cooling Rack Section: W135 x H150 x D220 mm, 3.6 kg
4 - 35°C
AC 100 V, 200 VA, 50/60 Hz
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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A

(2-2) Model SIL-HTA/C

Product Name SIL-HTA (with sample cooler), SIL-HTC (with sample cooler)
Method Total sample volume injection and variable sample volume injection (zero sample loss during injection)
Injection Volume Setting Range 0.1 - 100 µL (standard)
1 - 2000 µL (optional): 0.1 µL steps for 0.1 - 0.9 µL and 1 µL steps for 1 - 2000 µL
Number of Samples Processed Using 1 mL vials: 350
Using 1.5 mL vials: 210 (SIL-HTA), 140 (SIL-HTC)
Using 4 mL vials: 100
Using micro-plates: 384 with four 96-well micro plates, 1536 with four 384-well micro plates
Using deep-well plates: 384 with four 96-well plates, 1536 with four 384-well plates
Injection Volume Reproducibility RSD < 0.3% (when injecting 10 µL, depending on given conditions)
Cross Contamination 0.01% or less (provided, analytical conditions are for caffeine)
Injection Volume Accuracy Within ±1% (50 µL, n = 6)
Injection Volume Accuracy Correction Possible
Number of Injection Iterations 1 - 99 per sample
Analysis Time Settings 0.01 - 9999.9 min (0.01 min steps up to 999.99 min, 0.1 min steps for 1000 min and above)
No. of Steps for Injection Conditions 102 steps maximum
Sample Suction Rate 0.1 - 15 µL/sec (0.1 µL/sec steps for 0.1 - 1.0 µL/sec rates, 1 µL/sec steps for 1 µL/sec and above)
Operating Pressure 20.0 MPa maximum
Sample Cooler Method Aluminum block is cooled via Peltier elements
(SIL-HTC only) Temp Setting 4 - 40 °C
Range (Can be controlled to 4°C, given a 30°C or less room temp and 60% or less humidity)
Amb. Operating 4 - 35 °C
Temp Range
Operating pH range pH1 - 14
Liquid Contact Surfaces Stainless steel (SUS 316), ceramics and Teflon
Ambient Operating Temp Range 4 - 35°C
Operating Humidity Range 70% Max.
Size and Weight W540 x H500 x D415 mm, 40 kg (SIL-HTA), 45 kg (SIL-HTC)
Power Supply Requirements AC 100 V, 300 VA, 50/60 Hz

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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A

(3) Column Oven

Product Name CTO-10Avp CTO-10AVvp CTO-10ASvp
Heating Method Forced-air circulation method Forced-air circulation method Block heating method
Temperature Setting 4 - 80 ºC, 1 °C steps 4 - 80 °C, 1 °C steps 4 - 80 °C, 1 °C steps
Range
Temperature ±0.1 °C ±0.1 °C ±0.1 °C
Adjustability
Temperature Setting (room temp + 10) °C - 80 °C (room temp - 10) °C - 80 °C (room temp - 10) °C - (room temp + 60) °C
Range
Interior Oven W220 x H365 x D95 mm W220 x H365 x D95 mm Two 25 cm columns
Dimensions
Precise Temp Air circulation fan rotation speed adjustments Air circulation fan rotation speed adjustments Pre-heating function (capacity: two 2 µL
Adjustability channels)
Safety Measures (1) Max. operating temperature can be set to (1) Max. operating temperature can be set to (1) Max. operating temperature can be set to
prevent overheating prevent overheating prevent overheating
(2) A temperature fuse is included internally (2) A temperature fuse is included internally (2) A temperature fuse is included internally
(3) A flammable solvent leak sensor is included (3) A flammable solvent leak sensor is included (3) A flammable solvent leak sensor is included
internally internally internally
Time Program Functions Temp settings can be changed and temp Temp settings can be changed and temp Temp settings can be changed and temp
control started / stopped. control started / stopped. control started / stopped.
320 steps, 0.1 - 999.9 minutes 320 steps, 0.1 - 999.9 minutes 320 steps, 0.1 - 999.9 minutes
Size W260 x H415 x D420 mm W260 x H415 x D420 mm W130 x H415 x D420 mm
Weight 20 kg 23 kg 12 kg
Amb. Operating Temp Range 4 - 35 °C 4 - 35 °C 4 - 35 °C
Power Supply AC 100 V, 550 VA, 50/60 Hz AC 100 V, 550 VA, 50/60 Hz AC 100 V, 120 VA, 50/60 Hz

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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A

(4) UV-VIS Detector

Product Name SPD-10Avp / SPD-10AVvp
Light Source D2 lamp (SPD-10Avp), D2 and W lamps (SPD-10AVvp)
Wavelength Range 190 ~ 600 nm (SPD-10Avp), 190 - 900 nm (SPD-10AVvp)
Spectral Bandwidth 8 nm
Wavelength Precision ±1 nm
Wavelength Reproducibility ±0.1 nm
Drift 2x10-4 AU/h or less (at constant room temperature)
(SPD-10Avp: 250 nm, SPD-10AVvp: 250 nm / 600 nm, air inside cell)
Noise Level ±0.35x10-5 AU or less (for a 2 sec response)
±0.5x10-5 AU or less (for a 1 sec response)
(SPD-10Avp: 250 nm, SPD-10Avp: 250 nm / 600 nm, air inside cell)
Response Selectable in 10 steps (0.05, 0.1, 0.5, 1.0, 1.5, 2.0, 3.0, 6.0, 8.0, 10.0 sec)
Range Can be set to 0.0001 - 2.56 AUFS in steps of 0.0001 AUFS
Zero Adjustments Auto-zero feature, baseline shift
Cells (optical pathlength, volume, Standard Cell: 10 mm, 8 µL, 12 MPa (approx. 120 kgf/cm2)
max. pressure) (Optional preparative cells, semi-micro cells, inert cells, and temperature adjustable cells are available)
Simultaneous Dual-wavelength SPD-10Avp: Two wavelengths from ranges 190 - 370 nm or 371 - 600nm can be selected
Monitoring SPD-10AVvp: Two wavelengths from ranges 190 - 370 nm, 371 - 700 nm or 701 - 900 nm can be selected
Ratio Chromatogram A ratio of the two wavelength settings is output
Wavelength Scanning Function Available
Time Program Possible via either the detector itself or the system controller.
Wavelength (two wavelengths), auto-zero, range, markers, response, wavelength scan, events, polarity, lamp on/off and loop
Polarity Switching Possible
Size and Weight W260 x H140 x D420 mm, 13 kg
Amb. Operating Temp Range 4 - 35 °C
Power Supply Requirements AC 100 V, 150 VA, 50/60 Hz

7.2 Standard LC Workstation and PC System Specifications

Standard specifications for the CLASS-VP and PC system from Shimadzu Corporation (as of April 2002).
Product Name FMV-6750CL7s
OS Windows NT 4.0 Workstation SP6
CPU Duron 750 MHz
Memory Standard 128MB, Expandable 128MB Total 256MB
HDD 30.7 GB
CD-ROM x24 max.
Network Interface 100BASE-TX/10BASE-T LAN Interface
LCD Monitor VL-150VA
Pre-installed Software ・ CLASS-VP
・ CLASS-VP Agent
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Shimadzu HPLC Systems Created on: June 07, 2002 – Ver. A

・ CLASS-Agent Manager
・ LC10/VP File Converter
・ Adobe Acrobat 5.0 (installed separately)
Main Features ・ The CLASS-VP workstation PC comes with a two-year warranty (free repair) and a maintenance contract from Fujitsu Support and
Service. If any trouble should occur with the PC, dedicated support personnel will work to restore the system promptly and reliably.
・ Analytical instrument control and data processing
・ User management features
・ Can generate analytical reports in PDF file format
・ Can generate files in Andi format (AIA files）
・ Audit trail features
・ Automatic record keeping of analytical data

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