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CHAPTER 4: BIOCATALYSIS
LEARNING OUTCOMES:
a) State the properties of enzymes.
b) State the six classes of enzyme according to IUBMB classification
c) Explain how enzymes lower activation energy.
d) Illustrate to explain the mechanism of action based on an induced fit model.
e) State the factors that affect the enzymatic reaction
MAIN IDEAS
EXPLANATION NOTES
/KEY POINT
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Biology Student’s Companion Resources SB 025
Activation ● The initial energy needed to start a chemical reaction is called the
energy free energy of activation, or activation energy (EA).
● Activation energy is often supplied in the form of heat from the
surroundings
How do ● Enzyme lowers the activation energy (EA) (or barrier) necessary to
enzymes initiate a chemical reaction
works
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Biology Student’s Companion Resources SB 025
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Biology Student’s Companion Resources SB 025
Induced Fit Model
Mechanism of
enzyme action
Induced Fit
Model
2. Products that formed no longer bind to the active site are then released.
❖ Enzymes return to their original shape.
❖ Ready to bind the next substrate molecule
Effect of Each enzyme has an optimal temperature at which an enzyme produces the
temperature highest production rate.
▪ Optimal conditions favor the most active shape for the enzyme.
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▪ Effects of the movement of enzymes.
▪ Increase temperature: The rate of enzymatic reactions will increase.
▪ Substrates collide with active sites more frequently.
▪ Fastest conversion of the reactants to product molecules.
▪ Above optimal temperature: Enzymes become denatured
▪ Because high temperature causes the atoms making up the enzyme
molecule to vibrate or have great kinetic energy
▪ The vibration breaks the hydrogen bonds and other bonds that hold
the enzyme
▪ Rate of reaction drops sharply
LEARNING OUTCOMES:
● Define cofactor
● Explain the three types of cofactors and functions of:
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Biology Student’s Companion Resources SB 025
● i. Metal ion activators (example Mg2+).
● ii. Coenzyme (example NAD+)
● iii. Prosthetic group (example haem group)
MAIN IDEAS
EXPLANATION NOTES
/KEY POINT
No Cofactors Examples
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Biology Student’s Companion Resources SB 025
Prosthetic ● A metal or Coenzymes that are covalently or non covalently
group bound very tightly to the enzyme.
● Example: prosthetic group of electron carrier cytochrome and
enzyme catalase. FAD (riboflavin) - prosthetic group of the
electron carrier cytochrome. It takes part in oxidation-
reduction reactions.
LEARNING OUTCOMES:
● Define inhibitors.
● Explain the roles and types of inhibitors:
i. competitive inhibitors
ii. non competitive inhibitors.
● Analyze graphs related to competitive and non competitive inhibition.
MAIN IDEAS
EXPLANATION NOTES
/KEY POINT
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Biology Student’s Companion Resources SB 025
● Enzymes can be reused again (do not destroy and do not
denature).
● Causes No Permanent Damage to the enzyme
Graph related
to competitive
and non-
competitive
inhibition.
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