BACHELOR OF SCIENCE IN MEDICAL

LABORATORY SCIENCE:
BIOCHEMISTRY FOR MEDICAL
LABORATORY SCIENCE (LABORATORY)
COURSE MODULE COURSE UNIT WEEK
5 4 7
Enzymes

 Read course and unit objectives

 Read study guide prior to class attendance
 Read required learning resources; refer to unit
terminologies for jargons
 Proactively participate in classroom discussions
 Participate in weekly discussion board (Canvas)
 Answer and submit course unit tasks

At the end of this unit, the students are expected to:

Cognitive:
1. Identify the nature of enzymes
2. Describe the effects of temperature and pH to enzyme activity

Affective:
 1. Listen attentively during class discussions
2. Demonstrate tact and respect when challenging other people’s opinions and ideas
3. Accept comments and reactions of classmates on one’s opinions openly and graciously.
4. Perform simulations of biochemical tests on various bio-organic substances.

Psychomotor:
1. Participate actively during class discussions
2. Confidently express personal opinion and thoughts in front of the class

OBJECTIVES
1) To isolate and determine the properties of catalase
2) To determine the effect of sulfides on catalase activity
3) To prove the presence of a proteins factor in enzymes
4) To determine the effect of temperature on enzyme activity

ANALYTICAL PHASE
OBSERVATIONS

1. What happened after adding 6% H2O2 to the extract?

2. What is the color of the solution after adding Iodine solution?
3. EFFECT OF pH
A (0 B (2 C (4 D (6 E (8 mins) F (10 G (12 H (14
mins) mins) mins) mins) mins) mins) mins)
5
6.
8
8
4. EFFECT OF TEMPERATURE
A (0 B (2 C (4 D (6 E (8 mins) F (10 G (12 H (14
mins) mins) mins) mins) mins) mins) mins)
5C
37C
50C

Critical Thinking

1. Write the equation for the reaction catalyzed by catalase.

 (Hydrogen Peroxide) 2H2O2 catalase -> (Water) 2H2O + (Oxygen) O2
 2. How does an enzyme alter the rate of a chemical reaction?
 Enzymes are catalysts in biology. Catalysts reduce reaction activation energy. The smaller the
activation energy, the faster the reaction. As a result, enzymes reduce the activation energy of
processes.
3. What is the optimum pH and temperature for salivary amylase?
 optimum temperature (32–37 °C) Optimum pH 7.0
4. What effects do increased pH and increased temperature have on the activity of salivary
amylase?
Increased pH: Salivary amylase did not work in these tubes since pH 5 is acidic and pH 8 is
alkaline, however, the enzyme did act in the tube with pH 6.8 (mildly acidic) and digested
the starch.
Increased temperature: Denatured enzymes are deactivated at low temperatures.
5. In summary, what factors affect enzyme activity?
 Temperature, pH, and concentration can all influence enzyme activity. Enzymes perform best
within specified temperature and pH ranges, and poor conditions can cause an enzyme's
ability to bind to a substrate to be lost.

Group 5:
Flores, Jelaika B.
Pangandamun, Casamirah
Pangilinan, Michaella Joy
Punzalan, Camille Renzel
Valencia, Jean Danielle