glutamina

materia quimica analitica

presentado por karoll Dayana Lopez Ariza

universidAD DE CALDAS

2020
 beneficios

SUPLEMENTOS DE LA GLUTAMINA
acciones biologicas
 riesgos de consumir glutamina

disociasion
Glutamina

propied
 biosintes

AMINA
Pka1 2.17
Pka2 9.13
propiedades fisicoquimicas
biosintesis
ka1 0.0068 ka1' 147.91
ka2 7E-10 ka2' 1E+09
 titulación acido-base

titulante NaOH 0.01

Analito Glutamina 0.01

Va=
20 ml
Vb=
20 ml
Ca=
0.001 M
Cb=
0.001 M

K1 K2
0.0068 7.4131E-10

Ve1=
20 ml
Ve2=
40 ml
Kw 1.00E-14
eje x
Vb/ml [H2A] [HA-]
zona I 0 0.001 0.0055
1 0.0009 0.0000
2 0.0008 0.0001
3 0.0007 0.0001
4 0.0007 0.0002
5 0.0006 0.0002
6 0.0005 0.0002
7 0.0005 0.0003
8 0.0004 0.0003
 9 0.0004 0.0003
10 0.0003 0.0003
zona II 11 0.0003 0.0004
12 0.0003 0.0004
13 0.0002 0.0004
14 0.0002 0.0004
15 0.0001 0.0004
16 0.0001 0.0004
17 0.0001 0.0005
18 0.0001 0.0005
19 0.0000 0.0005
zona III 20 0.0000 0.0005
21 0.0005
22 0.0004
23 0.0004
24 0.0004
25 0.0003
26 0.0003
27 0.0003
28 0.0003
zona IV 29 0.0002
30 0.0002
31 0.0002
32 0.0002
33 0.0001
34 0.0001
35 0.0001
36 0.0001
37 0.0001
38 0.0000
39 0.0000
zona V 40 0.0000
41
42
43
44
45
46
47
48
zona 49
50
51
52
53
54
55
56
57
58
59
60
-base

M Bureta
M Erlemmeyer

12.00

10.00

8.00

PH SA
6.00

4.00

2.00

6.76083E-05 kb1 CHA/Ka 0.147910839 0.00

0 10
Pka1 2.17
pka2 9.13 Kb1 1.35E-05

[A2-] [H+] CA [OH-]CA PH CA PH CA2 [H+]CA2 [OH-]CA2

0 0.00551 1.81E-12 2.26 2.59 0.0026 3.85E-12
0.12846 7.78E-14 0.89 2.61 0.0025 4.04E-12
0.06085 1.64E-13 1.22 2.63 0.0024 4.25E-12
0.03831 2.61E-13 1.42 2.65 0.0022 4.47E-12
0.02704 3.70E-13 1.57 2.67 0.0021 4.71E-12
0.02028 4.93E-13 1.69 2.70 0.0020 4.97E-12
0.01578 6.34E-13 1.80 2.72 0.0019 5.24E-12
0.01256 7.96E-13 1.90 2.74 0.0018 5.54E-12
0.01014 9.86E-13 1.99 2.77 0.0017 5.87E-12
 0.00826 1.21E-12 2.08 2.80 0.0016 6.24E-12
0.00676 1.48E-12 2.17 2.82 0.0015 6.66E-12
0.00553 1.81E-12 2.26 2.85 0.0014 7.14E-12
0.00451 2.22E-12 2.35 2.89 0.0013 7.69E-12
0.00364 2.75E-12 2.44 2.92 0.0012 8.35E-12
0.00290 3.45E-12 2.54 2.96 0.0011 9.16E-12
0.00225 4.44E-12 2.65 3.01 0.0010 1.02E-11
0.00169 5.92E-12 2.77 3.06 0.0009 1.15E-11
0.00119 8.38E-12 2.92 3.13 0.0007 1.35E-11
0.00075 1.33E-11 3.12 3.22 0.0006 1.68E-11
0.00036 2.81E-11 3.45 3.38 0.0004 2.40E-11
7E-06 1.45E-09 5.16 5.65 2.23872114E-06 4.46684E-09
0.0000 1.4084895E-08 1.2736831E-15 7.85
0.0000 6.51294E-09 1.2215646E-15 8.19
0.0001 4.0053739E-09 1.1908509E-15 8.40
0.0001 2.7630654E-09 1.1684142E-15 8.56
0.0001 2.026248E-09 1.1503102E-15 8.69
0.0001 1.5417104E-09 1.1348165E-15 8.81
0.0002 1.2009677E-09 1.1210173E-15 8.92
0.0002 9.4980375E-10 1.1083567E-15 9.02
0.0002 7.5812E-10 1.0964597E-15 9.12
0.0002 6.078744E-10 1.0850475E-15 9.22
0.0003 4.8759978E-10 1.0738905E-15 9.31
0.0003 3.8966308E-10 1.0627771E-15 9.41
0.0003 3.0878961E-10 1.0514874E-15 9.51
0.0003 2.4122E-10 1.0397619E-15 9.62
0.0004 1.8420436E-10 1.027253E-15 9.73
0.0004 1.3568625E-10 1.0134316E-15 9.87
0.0004 9.4098204E-11 9.9736509E-16 10.03
0.0004 5.8225517E-11 9.7705039E-16 10.23
0.0005 2.7113042E-11 9.4635835E-16 10.57
0.0005 1.4725505E-10 1.0170943E-15 9.83
0.0005 6.1E-10 1.085226E-15 9.21
0.0005 3.1E-10 3.23E-05 9.51
0.0006 2.1E-10 4.76E-05 9.68
0.0006 1.6E-10 6.25E-05 9.80
0.0006 1.3E-10 7.69E-05 9.89
0.0006 1.1E-10 9.09E-05 9.96
0.0007 9.5714286E-11 1.04E-04 10.02
0.0007 8.5E-11 1.18E-04 10.07
0.0007 7.6666667E-11 1.30E-04 10.12
0.0007 7E-11 1.43E-04 10.15
0.0008 6.4545455E-11 1.55E-04 10.19
0.0008 6E-11 1.67E-04 10.22
0.0008 5.6153846E-11 1.78E-04 10.25
0.0008 5.2857143E-11 1.89E-04 10.28
0.0009 5E-11 2.00E-04 10.30
0.0009 4.75E-11 2.11E-04 10.32
0.0009 4.5294118E-11 2.21E-04 10.34
0.0009 4.3333333E-11 2.31E-04 10.36
0.0010 4.1578947E-11 2.41E-04 10.38
0.0010 4E-11 2.50E-04 10.40
 eje y eje y
12.00 0.5
0.45
10.00 0.4
0.35
8.00 0.3
0.25
PH SA

6.00
0.2
4.00 0.15
0.1
2.00 0.05
0
0.00 0.00 2.00 4.00 6.00
0 10 20 30 40 50 60 70
αH2A
Volumen de titulante NaOH/ml

eje y
PH SA a b c [H+]SA [OH-]SA αH2A
2.26 0.005510 1.81E-12 0.44902232
3.09 1 0.0068 - 0.000006 0.0008 1.24E-11 0.10623254
3.14 1 0.0069 - 0.000006 0.0007 1.37E-11 0.09740773
3.18 1 0.0069 - 0.000005 0.0007 1.51E-11 0.08913753
3.22 1 0.0069 - 0.000005 0.0006 1.67E-11 0.08136997
3.27 1 0.0070 - 0.000004 0.0005 1.85E-11 0.07405951
3.31 1 0.0070 - 0.000004 0.0005 2.05E-11 0.06716608
3.36 1 0.0070 - 0.000003 0.0004 2.29E-11 0.06065423
3.41 1 0.0070 - 0.000003 0.0004 2.57E-11 0.0544925
 3.46 1 0.0071 - 0.000003 0.0003 2.89E-11 0.04865288
3.52 1 0.0071 - 0.000002 0.0003 3.28E-11 0.04311028
3.58 1 0.0071 - 0.000002 0.0003 3.76E-11 0.03784223
3.64 1 0.0071 - 0.000002 0.0002 4.36E-11 0.03282849
3.71 1 0.0072 - 0.000001 0.0002 5.13E-11 0.0280508
3.79 1 0.0072 - 0.000001 0.0002 6.15E-11 0.02349261
3.88 1 0.0072 - 0.000001 0.0001 7.58E-11 0.01913892
3.99 1 0.0072 - 0.000001 0.0001 9.73E-11 0.0149761
4.12 1 0.0072 - 0.000001 0.0001 1.33E-10 0.01099169
4.31 1 0.0072 - 0.000000 0.0000 2.05E-10 0.00717432
4.62 1 0.0072 - 0.000000 0.0000 4.19E-10 0.00351357
6.23 5.9535048E-07 1.68E-08 8.79415E-05
7.85 1.4084895E-08 7.10E-07 1.97914E-06
8.19 6.51294E-09 1.54E-06 8.64891E-07
8.40 4.0053739E-09 2.50E-06 4.99914E-07
8.56 2.7630654E-09 3.62E-06 3.22234E-07
8.69 2.026248E-09 4.94E-06 2.19426E-07
8.81 1.5417104E-09 6.49E-06 1.53991E-07
8.92 1.2009677E-09 8.33E-06 1.09838E-07
9.02 9.4980375E-10 1.05E-05 7.89032E-08
9.12 7.5812E-10 1.32E-05 5.66956E-08
9.22 6.078744E-10 1.65E-05 4.05094E-08
9.31 4.8759978E-10 2.05E-05 2.86159E-08
9.41 3.8966308E-10 2.57E-05 1.98576E-08
9.51 3.0878961E-10 3.24E-05 1.34306E-08
9.62 2.4122E-10 4.15E-05 8.75953E-09
9.73 1.8420436E-10 5.43E-05 5.42271E-09
9.87 1.3568625E-10 7.37E-05 3.10509E-09
10.03 9.4098204E-11 1.06E-04 1.5677E-09
10.23 5.8225517E-11 1.72E-04 6.27175E-10
10.57 2.7113042E-11 3.69E-04 1.415E-10
9.83 1 1.35E-05 -6.58030674E-09 1.47E-10 6.79E-05 3.60953E-09
9.21 6.10E-10 2E-05 4.07291E-08
9.51 3.10E-10 3E-05 1.35205E-08
9.68 2.10E-10 5E-05 6.85672E-09
9.80 1.60E-10 6E-05 4.20113E-09
9.89 1.30E-10 8E-05 2.86889E-09
9.96 1.10E-10 9E-05 2.10231E-09
10.02 9.57E-11 1E-04 1.61888E-09
10.07 8.50E-11 1E-04 1.29329E-09
10.12 7.67E-11 1E-04 1.06285E-09
10.15 7.00E-11 1E-04 8.93324E-10
10.19 6.45E-11 2E-04 7.6467E-10
10.22 6.00E-11 2E-04 6.6451E-10
10.25 5.62E-11 2E-04 5.84855E-10
10.28 5.29E-11 2E-04 5.2035E-10
10.30 5.00E-11 2E-04 4.67297E-10
10.32 4.75E-11 2E-04 4.23072E-10
10.34 4.53E-11 2E-04 3.85769E-10
10.36 4.33E-11 2E-04 3.53974E-10
10.38 4.16E-11 2E-04 3.26623E-10
10.40 4.00E-11 3E-04 3.02898E-10
 eje y

2.00 4.00 6.00 8.00 10.00 12.00

αH2A

αHA- αA-2
81.49556 7.4131E-08
132.197773 8.245E-07
133.503045 9.17041E-07
134.726281 1.02057E-06
135.87517 1.13715E-06
136.956446 1.26936E-06
137.976038 1.42055E-06
138.939185 1.5951E-06
139.850541 1.79884E-06
140.714249 2.03971E-06
141.534016 2.32885E-06
142.313166 2.68234E-06
143.054687 3.1243E-06
143.761276 3.69265E-06
144.435369 4.45057E-06
145.079169 5.51179E-06
145.694661 7.10376E-06
146.283606 9.75724E-06
146.847452 1.50644E-05
147.386561 3.09861E-05
147.713903 0.00124351
140.515019 0.0499999
132.795748 0.10218969
124.8109 0.15617426
116.62197 0.21153839
108.29184 0.26785708
99.883304 0.32470583
91.45762 0.38167049
83.0732043 0.43835613
74.7845118 0.49439459
66.6411445 0.54945053
58.6872021 0.60322579
50.9608762 0.65546217
43.4942727 0.70594261
36.3134392 0.75449101
29.4385648 0.80097087
22.8843184 0.84528302
16.6602868 0.88736264
10.771482 0.92717584
5.21888502 0.96471601
24.5121193 0.83427773
66.7689811 0.54858625
43.6144894 0.70512985
32.6510476 0.77925182
26.2570345 0.82248066
22.068384 0.85079941
19.1119423 0.87078741
16.9136982 0.88564936
15.2151343 0.89713307
13.8632654 0.90627282
12.7617749 0.91371981
11.8469999 0.91990445
11.075174 0.92512263
10.4152182 0.92958448
9.84445407 0.93344332
9.34594493 0.93681366
8.90678705 0.93978273
8.51697662 0.94241817
8.16863846 0.94477323
7.85548845 0.94689038
7.57245104 0.94880395
 titulación acido-base

titulante NaOH 0.01

Analito Glutamina 0.01

Va=
20 ml
Vb=
20 ml
Ca=
0.01 M
Cb=
0.01 M

K1 K2
0.0068 7.4131E-10

Ve1=
20 ml
Ve2=
40 ml
Kw 1.00E-14
eje x
Vb/ml [H2A] [HA-]
zona I 0 0.01 0.0055
1 0.0090 0.0005
2 0.0082 0.0009
3 0.0074 0.0013
4 0.0067 0.0017
5 0.0060 0.0020
6 0.0054 0.0023
7 0.0048 0.0026
8 0.0043 0.0029
 9 0.0038 0.0031
10 0.0033 0.0033
zona II 11 0.0029 0.0035
12 0.0025 0.0038
13 0.0021 0.0039
14 0.0018 0.0041
15 0.0014 0.0043
16 0.0011 0.0044
17 0.0008 0.0046
18 0.0005 0.0047
19 0.0003 0.0049
zona III 20 0.0000 0.0050
21 0.0046
22 0.0043
23 0.0040
24 0.0036
25 0.0033
26 0.0030
27 0.0028
28 0.0025
zona IV 29 0.0022
30 0.0020
31 0.0018
32 0.0015
33 0.0013
34 0.0011
35 0.0009
36 0.0007
37 0.0005
38 0.0003
39 0.0002
zona V 40 0.0000
41
42
43
44
45
46
47
48
zona 49
50
51
52
53
54
55
56
57
58
59
60
-base

M Bureta
M Erlemmeyer

12.00

10.00

8.00

PH SA
6.00

4.00

2.00

6.76083E-05 kb1 CHA/Ka 1.479108388 0.00

0 10
Pka1 2.17
pka2 9.13 Kb1 1.35E-05

[A2-] [H+] CA [OH-]CA PH CA PH CA2 [H+]CA2 [OH-]CA2

0 0.00551 1.81E-12 2.26 2.09 0.0082 1.22E-12
0.12846 7.78E-14 0.89 2.11 0.0078 1.28E-12
0.06085 1.64E-13 1.22 2.13 0.0074 1.34E-12
0.03831 2.61E-13 1.42 2.15 0.0071 1.41E-12
0.02704 3.70E-13 1.57 2.17 0.0067 1.49E-12
0.02028 4.93E-13 1.69 2.20 0.0064 1.57E-12
0.01578 6.34E-13 1.80 2.22 0.0060 1.66E-12
0.01256 7.96E-13 1.90 2.24 0.0057 1.75E-12
0.01014 9.86E-13 1.99 2.27 0.0054 1.86E-12
 0.00826 1.21E-12 2.08 2.30 0.0051 1.97E-12
0.00676 1.48E-12 2.17 2.32 0.0047 2.11E-12
0.00553 1.81E-12 2.26 2.35 0.0044 2.26E-12
0.00451 2.22E-12 2.35 2.39 0.0041 2.43E-12
0.00364 2.75E-12 2.44 2.42 0.0038 2.64E-12
0.00290 3.45E-12 2.54 2.46 0.0035 2.90E-12
0.00225 4.44E-12 2.65 2.51 0.0031 3.22E-12
0.00169 5.92E-12 2.77 2.56 0.0027 3.65E-12
0.00119 8.38E-12 2.92 2.63 0.0023 4.27E-12
0.00075 1.33E-11 3.12 2.72 0.0019 5.30E-12
0.00036 2.81E-11 3.45 2.88 0.0013 7.60E-12
1E-05 7.43E-10 4.87 5.65 2.23872114E-06 4.46684E-09
0.0002 1.4084895E-08 1.2736831E-15 7.85
0.0005 6.51294E-09 1.2215646E-15 8.19
0.0007 4.0053739E-09 1.1908509E-15 8.40
0.0010 2.7630654E-09 1.1684142E-15 8.56
0.0012 2.026248E-09 1.1503102E-15 8.69
0.0015 1.5417104E-09 1.1348165E-15 8.81
0.0017 1.2009677E-09 1.1210173E-15 8.92
0.0020 9.4980375E-10 1.1083567E-15 9.02
0.0022 7.5812E-10 1.0964597E-15 9.12
0.0024 6.078744E-10 1.0850475E-15 9.22
0.0027 4.8759978E-10 1.0738905E-15 9.31
0.0029 3.8966308E-10 1.0627771E-15 9.41
0.0032 3.0878961E-10 1.0514874E-15 9.51
0.0034 2.4122E-10 1.0397619E-15 9.62
0.0037 1.8420436E-10 1.027253E-15 9.73
0.0039 1.3568625E-10 1.0134316E-15 9.87
0.0041 9.4098204E-11 9.9736509E-16 10.03
0.0044 5.8225517E-11 9.7705039E-16 10.23
0.0046 2.7113042E-11 9.4635835E-16 10.57
0.0049 4.1131932E-11 9.6285119E-16 10.39
0.0051 6.1E-11 9.7898413E-16 10.21
0.0054 3.1E-11 3.23E-04 10.51
0.0056 2.1E-11 4.76E-04 10.68
0.0059 1.6E-11 6.25E-04 10.80
0.0061 1.3E-11 7.69E-04 10.89
0.0063 1.1E-11 9.09E-04 10.96
0.0066 9.5714286E-12 1.04E-03 11.02
0.0068 8.5E-12 1.18E-03 11.07
0.0071 7.6666667E-12 1.30E-03 11.12
0.0073 7E-12 1.43E-03 11.15
0.0076 6.4545455E-12 1.55E-03 11.19
0.0078 6E-12 1.67E-03 11.22
0.0080 5.6153846E-12 1.78E-03 11.25
0.0083 5.2857143E-12 1.89E-03 11.28
0.0085 5E-12 2.00E-03 11.30
0.0088 4.75E-12 2.11E-03 11.32
0.0090 4.5294118E-12 2.21E-03 11.34
0.0093 4.3333333E-12 2.31E-03 11.36
0.0095 4.1578947E-12 2.41E-03 11.38
0.0098 4E-12 2.50E-03 11.40
 eje y eje y
12.00 0.5
0.45
10.00 0.4
0.35
8.00 0.3
0.25
PH SA

6.00
0.2
4.00 0.15
0.1
2.00 0.05
0
0.00 0.00 2.00 4.00 6.00
0 10 20 30 40 50 60 70
αH2A
Volumen de titulante NaOH/ml

eje y
PH SA a b c [H+]SA [OH-]SA αH2A
2.26 0.005510 1.81E-12 0.44902232
2.30 1 0.0072 - 0.000061 0.0050 2.00E-12 0.42509554
2.34 1 0.0077 - 0.000055 0.0045 2.21E-12 0.40136969
2.39 1 0.0081 - 0.000050 0.0041 2.44E-12 0.37783472
2.43 1 0.0084 - 0.000045 0.0037 2.69E-12 0.35448132
2.48 1 0.0088 - 0.000041 0.0033 2.99E-12 0.3313009
2.52 1 0.0091 - 0.000036 0.0030 3.32E-12 0.30828547
2.57 1 0.0094 - 0.000033 0.0027 3.70E-12 0.28542763
2.62 1 0.0096 - 0.000029 0.0024 4.15E-12 0.26272047
 2.67 1 0.0099 - 0.000026 0.0021 4.68E-12 0.24015755
2.73 1 0.0101 - 0.000023 0.0019 5.31E-12 0.21773284
2.78 1 0.0103 - 0.000020 0.0016 6.09E-12 0.1954407
2.85 1 0.0105 - 0.000017 0.0014 7.06E-12 0.17327585
2.92 1 0.0107 - 0.000014 0.0012 8.30E-12 0.15123333
3.00 1 0.0109 - 0.000012 0.0010 9.96E-12 0.12930845
3.09 1 0.0110 - 0.000010 0.0008 1.23E-11 0.10749682
3.20 1 0.0112 - 0.000008 0.0006 1.58E-11 0.08579429
3.33 1 0.0114 - 0.000005 0.0005 2.16E-11 0.06419694
3.52 1 0.0115 - 0.000004 0.0003 3.32E-11 0.04270106
3.83 1 0.0116 - 0.000002 0.0001 6.80E-11 0.02130316
5.84 1.4616759E-06 6.84E-09 0.00021604
7.85 1.4084895E-08 7.10E-07 1.97914E-06
8.19 6.51294E-09 1.54E-06 8.64891E-07
8.40 4.0053739E-09 2.50E-06 4.99914E-07
8.56 2.7630654E-09 3.62E-06 3.22234E-07
8.69 2.026248E-09 4.94E-06 2.19426E-07
8.81 1.5417104E-09 6.49E-06 1.53991E-07
8.92 1.2009677E-09 8.33E-06 1.09838E-07
9.02 9.4980375E-10 1.05E-05 7.89032E-08
9.12 7.5812E-10 1.32E-05 5.66956E-08
9.22 6.078744E-10 1.65E-05 4.05094E-08
9.31 4.8759978E-10 2.05E-05 2.86159E-08
9.41 3.8966308E-10 2.57E-05 1.98576E-08
9.51 3.0878961E-10 3.24E-05 1.34306E-08
9.62 2.4122E-10 4.15E-05 8.75953E-09
9.73 1.8420436E-10 5.43E-05 5.42271E-09
9.87 1.3568625E-10 7.37E-05 3.10509E-09
10.03 9.4098204E-11 1.06E-04 1.5677E-09
10.23 5.8225517E-11 1.72E-04 6.27175E-10
10.57 2.7113042E-11 3.69E-04 1.415E-10
10.39 1 1.35E-05 -6.58030674E-08 4.11E-11 2.43E-04 3.1982E-10
10.21 6.10E-11 2E-04 6.85989E-10
10.51 3.10E-11 3E-04 1.84048E-10
10.68 2.10E-11 5E-04 8.55671E-11
10.80 1.60E-11 6E-04 5E-11
10.89 1.30E-11 8E-04 3.31388E-11
10.96 1.10E-11 9E-04 2.37897E-11
11.02 9.57E-12 1E-03 1.8046E-11
11.07 8.50E-12 1E-03 1.42524E-11
11.12 7.67E-12 1E-03 1.16077E-11
11.15 7.00E-12 1E-03 9.68533E-12
11.19 6.45E-12 2E-03 8.24074E-12
11.22 6.00E-12 2E-03 7.12527E-12
11.25 5.62E-12 2E-03 6.24427E-12
11.28 5.29E-12 2E-03 5.53505E-12
11.30 5.00E-12 2E-03 4.95474E-12
11.32 4.75E-12 2E-03 4.47315E-12
11.34 4.53E-12 2E-03 4.06854E-12
11.36 4.33E-12 2E-03 3.72489E-12
11.38 4.16E-12 2E-03 3.43019E-12
11.40 4.00E-12 3E-03 3.17529E-12
 eje y

2.00 4.00 6.00 8.00 10.00 12.00

αH2A

αHA- αA-2
81.49556 7.4131E-08
85.0345884 8.5252E-08
88.5438961 9.78977E-08
92.0249719 1.12334E-07
95.4791903 1.28891E-07
98.9078234 1.47992E-07
102.312051 1.70177E-07
105.692969 1.96154E-07
109.0516 2.26866E-07
112.388896 2.63603E-07
115.705747 3.08167E-07
119.002987 3.63162E-07
122.281398 4.32497E-07
125.541713 5.2231E-07
128.784623 6.42837E-07
132.010774 8.125E-07
135.220775 1.06814E-06
138.415194 1.49573E-06
141.594541 2.35318E-06
144.759142 4.93001E-06
147.803923 0.0005068
140.515019 0.0499999
132.795748 0.10218969
124.8109 0.15617426
116.62197 0.21153839
108.29184 0.26785708
99.883304 0.32470583
91.45762 0.38167049
83.0732043 0.43835613
74.7845118 0.49439459
66.6411445 0.54945053
58.6872021 0.60322579
50.9608762 0.65546217
43.4942727 0.70594261
36.3134392 0.75449101
29.4385648 0.80097087
22.8843184 0.84528302
16.6602868 0.88736264
10.771482 0.92717584
5.21888502 0.96471601
7.77547374 0.94743135
11.245726 0.92396956
5.93703897 0.95986069
4.07462401 0.97245216
3.12497216 0.9788726
2.54913801 0.98276571
2.16269717 0.98537837
1.88540763 0.98725308
1.67674708 0.9886638
1.5140428 0.98976381
1.38361847 0.99064559
1.27673468 0.99136821
1.18754566 0.99197121
1.11199324 0.992482
1.04717207 0.99292025
0.9909474 0.99330037
0.94171549 0.99363322
0.89824816 0.9939271
0.85958894 0.99418847
0.82498187 0.99442244
0.79382158 0.99463311
 titulación acido-base

titulante NaOH 0.01

Analito Glutamina 0.01

Va=
20 ml
Vb=
20 ml
Ca=
0.1 M
Cb=
0.1 M

K1 K2
0.0068 7.4131E-10

Ve1=
20 ml
Ve2=
40 ml
Kw 1.00E-14
eje x
Vb/ml [H2A] [HA-]
zona I 0 0.1 0.0055
1 0.0905 0.0048
2 0.0818 0.0091
3 0.0739 0.0130
4 0.0667 0.0167
5 0.0600 0.0200
6 0.0538 0.0231
7 0.0481 0.0259
8 0.0429 0.0286
 9 0.0379 0.0310
10 0.0333 0.0333
zona II 11 0.0290 0.0355
12 0.0250 0.0375
13 0.0212 0.0394
14 0.0176 0.0412
15 0.0143 0.0429
16 0.0111 0.0444
17 0.0081 0.0459
18 0.0053 0.0474
19 0.0026 0.0487
zona III 20 0.0000 0.0500
21 0.0463
22 0.0429
23 0.0395
24 0.0364
25 0.0333
26 0.0304
27 0.0277
28 0.0250
zona IV 29 0.0224
30 0.0200
31 0.0176
32 0.0154
33 0.0132
34 0.0111
35 0.0091
36 0.0071
37 0.0053
38 0.0034
39 0.0017
zona V 40 0.0000
41
42
43
44
45
46
47
48
zona 49
50
51
52
53
54
55
56
57
58
59
60
-base

M Bureta
M Erlemmeyer

14.00

12.00

10.00

8.00

PH SA
6.00

4.00

2.00

6.76083E-05 kb1 CHA/Ka 14.79108388 0.00

0 10 20
Pka1 2.17 Volumen d
pka2 9.13 Kb1 1.35E-05

[A2-] [H+] CA [OH-]CA PH CA PH CA2 [H+]CA2 [OH-]CA2

0 0.00551 1.81E-12 2.26 1.59 0.0260 3.85E-13
0.12846 7.78E-14 0.89 1.61 0.0247 4.04E-13
0.06085 1.64E-13 1.22 1.63 0.0235 4.25E-13
0.03831 2.61E-13 1.42 1.65 0.0224 4.47E-13
0.02704 3.70E-13 1.57 1.67 0.0212 4.71E-13
0.02028 4.93E-13 1.69 1.70 0.0201 4.97E-13
0.01578 6.34E-13 1.80 1.72 0.0191 5.24E-13
0.01256 7.96E-13 1.90 1.74 0.0180 5.54E-13
0.01014 9.86E-13 1.99 1.77 0.0170 5.87E-13
 0.00826 1.21E-12 2.08 1.80 0.0160 6.24E-13
0.00676 1.48E-12 2.17 1.82 0.0150 6.66E-13
0.00553 1.81E-12 2.26 1.85 0.0140 7.14E-13
0.00451 2.22E-12 2.35 1.89 0.0130 7.69E-13
0.00364 2.75E-12 2.44 1.92 0.0120 8.35E-13
0.00290 3.45E-12 2.54 1.96 0.0109 9.16E-13
0.00225 4.44E-12 2.65 2.01 0.0098 1.02E-12
0.00169 5.92E-12 2.77 2.06 0.0087 1.15E-12
0.00119 8.38E-12 2.92 2.13 0.0074 1.35E-12
0.00075 1.33E-11 3.12 2.22 0.0060 1.68E-12
0.00036 2.81E-11 3.45 2.38 0.0042 2.40E-12
9E-06 1.13E-09 5.05 5.65 2.23872114E-06 4.46684E-09
0.0024 1.4084895E-08 1.2736831E-15 7.85
0.0049 6.51294E-09 1.2215646E-15 8.19
0.0073 4.0053739E-09 1.1908509E-15 8.40
0.0098 2.7630654E-09 1.1684142E-15 8.56
0.0122 2.026248E-09 1.1503102E-15 8.69
0.0146 1.5417104E-09 1.1348165E-15 8.81
0.0171 1.2009677E-09 1.1210173E-15 8.92
0.0195 9.4980375E-10 1.1083567E-15 9.02
0.0220 7.5812E-10 1.0964597E-15 9.12
0.0244 6.078744E-10 1.0850475E-15 9.22
0.0268 4.8759978E-10 1.0738905E-15 9.31
0.0293 3.8966308E-10 1.0627771E-15 9.41
0.0317 3.0878961E-10 1.0514874E-15 9.51
0.0341 2.4122E-10 1.0397619E-15 9.62
0.0366 1.8420436E-10 1.027253E-15 9.73
0.0390 1.3568625E-10 1.0134316E-15 9.87
0.0415 9.4098204E-11 9.9736509E-16 10.03
0.0439 5.8225517E-11 9.7705039E-16 10.23
0.0463 2.7113042E-11 9.4635835E-16 10.57
0.0488 1.2535581E-11 9.1727504E-16 10.90
0.0512 6.1E-12 8.9168918E-16 11.21
0.0537 3.1E-12 3.23E-03 11.51
0.0561 2.1E-12 4.76E-03 11.68
0.0585 1.6E-12 6.25E-03 11.80
0.0610 1.3E-12 7.69E-03 11.89
0.0634 1.1E-12 9.09E-03 11.96
0.0659 9.5714286E-13 1.04E-02 12.02
0.0683 8.5E-13 1.18E-02 12.07
0.0707 7.6666667E-13 1.30E-02 12.12
0.0732 7E-13 1.43E-02 12.15
0.0756 6.4545455E-13 1.55E-02 12.19
0.0780 6E-13 1.67E-02 12.22
0.0805 5.6153846E-13 1.78E-02 12.25
0.0829 5.2857143E-13 1.89E-02 12.28
0.0854 5E-13 2.00E-02 12.30
0.0878 4.75E-13 2.11E-02 12.32
0.0902 4.5294118E-13 2.21E-02 12.34
0.0927 4.3333333E-13 2.31E-02 12.36
0.0951 4.1578947E-13 2.41E-02 12.38
0.0976 4E-13 2.50E-02 12.40
 eje y eje y
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
0.00 2.00 4.00 6.00
0 10 20 30 40 50 60 70
αH2A
Volumen de titulante NaOH/ml

eje y
PH SA a b c [H+]SA [OH-]SA αH2A
2.26 0.005510 1.81E-12 0.44902232
1.71 1 0.0115 - 0.000612 0.0196 5.09E-13 0.74385068
1.77 1 0.0159 - 0.000553 0.0169 5.92E-13 0.71417656
1.84 1 0.0198 - 0.000500 0.0145 6.87E-13 0.68270831
1.90 1 0.0234 - 0.000451 0.0125 7.98E-13 0.64959679
1.97 1 0.0268 - 0.000406 0.0108 9.26E-13 0.61500184
2.03 1 0.0298 - 0.000364 0.0093 1.08E-12 0.57908292
2.10 1 0.0327 - 0.000326 0.0080 1.25E-12 0.54199238
2.16 1 0.0353 - 0.000290 0.0069 1.46E-12 0.50387123
 2.23 1 0.0378 - 0.000256 0.0059 1.70E-12 0.46484695
2.30 1 0.0401 - 0.000225 0.0050 2.00E-12 0.42503285
2.37 1 0.0422 - 0.000196 0.0042 2.37E-12 0.38452846
2.45 1 0.0443 - 0.000169 0.0035 2.83E-12 0.34342048
2.53 1 0.0462 - 0.000143 0.0029 3.42E-12 0.30178402
2.62 1 0.0479 - 0.000119 0.0024 4.22E-12 0.25968398
2.73 1 0.0496 - 0.000097 0.0019 5.33E-12 0.2171763
2.85 1 0.0512 - 0.000075 0.0014 7.01E-12 0.17430924
2.99 1 0.0527 - 0.000055 0.0010 9.80E-12 0.13112443
3.19 1 0.0541 - 0.000036 0.0006 1.54E-11 0.08765786
3.51 1 0.0555 - 0.000017 0.0003 3.22E-11 0.04394067
5.68 2.1014507E-06 4.76E-09 0.00031062
7.85 1.4084895E-08 7.10E-07 1.97914E-06
8.19 6.51294E-09 1.54E-06 8.64891E-07
8.40 4.0053739E-09 2.50E-06 4.99914E-07
8.56 2.7630654E-09 3.62E-06 3.22234E-07
8.69 2.026248E-09 4.94E-06 2.19426E-07
8.81 1.5417104E-09 6.49E-06 1.53991E-07
8.92 1.2009677E-09 8.33E-06 1.09838E-07
9.02 9.4980375E-10 1.05E-05 7.89032E-08
9.12 7.5812E-10 1.32E-05 5.66956E-08
9.22 6.078744E-10 1.65E-05 4.05094E-08
9.31 4.8759978E-10 2.05E-05 2.86159E-08
9.41 3.8966308E-10 2.57E-05 1.98576E-08
9.51 3.0878961E-10 3.24E-05 1.34306E-08
9.62 2.4122E-10 4.15E-05 8.75953E-09
9.73 1.8420436E-10 5.43E-05 5.42271E-09
9.87 1.3568625E-10 7.37E-05 3.10509E-09
10.03 9.4098204E-11 1.06E-04 1.5677E-09
10.23 5.8225517E-11 1.72E-04 6.27175E-10
10.57 2.7113042E-11 3.69E-04 1.415E-10
10.90 1 1.35E-05 -6.58030674E-07 1.25E-11 7.98E-04 3.08323E-11
11.21 6.10E-12 2E-03 7.36378E-12
11.51 3.10E-12 3E-03 1.90946E-12
11.68 2.10E-12 5E-03 8.77425E-13
11.80 1.60E-12 6E-03 5.09687E-13
11.89 1.30E-12 8E-03 3.36609E-13
11.96 1.10E-12 9E-03 2.41069E-13
12.02 9.57E-13 1E-02 1.82555E-13
12.07 8.50E-13 1E-02 1.43993E-13
12.12 7.67E-13 1E-02 1.17156E-13
12.15 7.00E-13 1E-02 9.76756E-14
12.19 6.45E-13 2E-02 8.30526E-14
12.22 6.00E-13 2E-02 7.17714E-14
12.25 5.62E-13 2E-02 6.28681E-14
12.28 5.29E-13 2E-02 5.57055E-14
12.30 5.00E-13 2E-02 4.98479E-14
12.32 4.75E-13 2E-02 4.49893E-14
12.34 4.53E-13 2E-02 4.0909E-14
12.36 4.33E-13 2E-02 3.74447E-14
12.38 4.16E-13 2E-02 3.44749E-14
12.40 4.00E-13 3E-02 3.1907E-14
 eje y

2.00 4.00 6.00 8.00 10.00 12.00 14.00

αH2A

αHA- αA-2
81.49556 7.4131E-08
37.8872587 9.67165E-09
42.2763822 1.25427E-08
46.9308781 1.6169E-08
51.8284296 2.07249E-08
56.9453971 2.64266E-08
62.2581938 3.35469E-08
67.7442845 4.24377E-08
73.3828139 5.35635E-08
79.1549263 6.75532E-08
85.0438608 8.52832E-08
91.0348959 1.08016E-07
97.1152078 1.37641E-07
103.273685 1.77126E-07
109.50073 2.31414E-07
115.788065 3.09396E-07
122.12855 4.28858E-07
128.51602 6.31293E-07
134.945137 1.04117E-06
141.4112 2.28087E-06
147.812752 0.00035253
140.515019 0.0499999
132.795748 0.10218969
124.8109 0.15617426
116.62197 0.21153839
108.29184 0.26785708
99.883304 0.32470583
91.45762 0.38167049
83.0732043 0.43835613
74.7845118 0.49439459
66.6411445 0.54945053
58.6872021 0.60322579
50.9608762 0.65546217
43.4942727 0.70594261
36.3134392 0.75449101
29.4385648 0.80097087
22.8843184 0.84528302
16.6602868 0.88736264
10.771482 0.92717584
5.21888502 0.96471601
2.45958547 0.98337116
1.20717655 0.99183848
0.61595552 0.99583563
0.41782147 0.99717518
0.31855442 0.99784631
0.25893003 0.99824942
0.21915366 0.99851834
0.19072885 0.99871051
0.16940306 0.99885469
0.15281207 0.99896686
0.1395366 0.99905662
0.12867308 0.99913006
0.11961892 0.99919128
0.11195685 0.99924308
0.10538872 0.99928748
0.09969587 0.99932597
0.09471427 0.99935965
0.09031846 0.99938937
0.08641086 0.99941579
0.0829144 0.99943943
0.07976745 0.99946071
 titulación acido-base

titulante NaOH 0.01

Analito Glutamina 0.01

Va=
20 ml
Vb=
20 ml
Ca=
1M
Cb=
1M

K1 K2
0.0068 7.4131E-10

Ve1=
20 ml
Ve2=
40 ml
Kw 1.00E-14
eje x
Vb/ml [H2A] [HA-]
zona I 0 1 0.0055
1 0.9048 0.0476
2 0.8182 0.0909
3 0.7391 0.1304
4 0.6667 0.1667
5 0.6000 0.2000
6 0.5385 0.2308
7 0.4815 0.2593
8 0.4286 0.2857
 9 0.3793 0.3103
10 0.3333 0.3333
zona II 11 0.2903 0.3548
12 0.2500 0.3750
13 0.2121 0.3939
14 0.1765 0.4118
15 0.1429 0.4286
16 0.1111 0.4444
17 0.0811 0.4595
18 0.0526 0.4737
19 0.0256 0.4872
zona III 20 0.0000 0.5000
21 0.4634
22 0.4286
23 0.3953
24 0.3636
25 0.3333
26 0.3043
27 0.2766
28 0.2500
zona IV 29 0.2245
30 0.2000
31 0.1765
32 0.1538
33 0.1321
34 0.1111
35 0.0909
36 0.0714
37 0.0526
38 0.0345
39 0.0169
zona V 40 0.0000
41
42
43
44
45
46
47
48
zona 49
50
51
52
53
54
55
56
57
58
59
60
-base

M Bureta
M Erlemmeyer
16.00

14.00

12.00

10.00

PH SA
8.00

6.00

4.00

2.00
6.76083E-05 kb1 CHA/Ka 147.9108388
0.00
Pka1 2.17 0 10
pka2 9.13 Kb1 1.35E-05

[A2-] [H+] CA [OH-]CA PH CA PH CA2 [H+]CA2 [OH-]CA2

0 0.00551 1.81E-12 2.26 1.09 0.0822 1.22E-13
0.12846 7.78E-14 0.89 1.11 0.0782 1.28E-13
0.06085 1.64E-13 1.22 1.13 0.0744 1.34E-13
0.03831 2.61E-13 1.42 1.15 0.0707 1.41E-13
0.02704 3.70E-13 1.57 1.17 0.0671 1.49E-13
0.02028 4.93E-13 1.69 1.20 0.0637 1.57E-13
0.01578 6.34E-13 1.80 1.22 0.0603 1.66E-13
0.01256 7.96E-13 1.90 1.24 0.0571 1.75E-13
0.01014 9.86E-13 1.99 1.27 0.0538 1.86E-13
 0.00826 1.21E-12 2.08 1.30 0.0506 1.97E-13
0.00676 1.48E-12 2.17 1.32 0.0475 2.11E-13
0.00553 1.81E-12 2.26 1.35 0.0443 2.26E-13
0.00451 2.22E-12 2.35 1.39 0.0411 2.43E-13
0.00364 2.75E-12 2.44 1.42 0.0379 2.64E-13
0.00290 3.45E-12 2.54 1.46 0.0345 2.90E-13
0.00225 4.44E-12 2.65 1.51 0.0311 3.22E-13
0.00169 5.92E-12 2.77 1.56 0.0274 3.65E-13
0.00119 8.38E-12 2.92 1.63 0.0234 4.27E-13
0.00075 1.33E-11 3.12 1.72 0.0189 5.30E-13
0.00036 2.81E-11 3.45 1.88 0.0132 7.60E-13
3E-06 3.20E-09 5.51 5.65 2.23872114E-06 4.46684E-09
0.0244 1.4084895E-08 1.2736831E-15 7.85
0.0488 6.51294E-09 1.2215646E-15 8.19
0.0732 4.0053739E-09 1.1908509E-15 8.40
0.0976 2.7630654E-09 1.1684142E-15 8.56
0.1220 2.026248E-09 1.1503102E-15 8.69
0.1463 1.5417104E-09 1.1348165E-15 8.81
0.1707 1.2009677E-09 1.1210173E-15 8.92
0.1951 9.4980375E-10 1.1083567E-15 9.02
0.2195 7.5812E-10 1.0964597E-15 9.12
0.2439 6.078744E-10 1.0850475E-15 9.22
0.2683 4.8759978E-10 1.0738905E-15 9.31
0.2927 3.8966308E-10 1.0627771E-15 9.41
0.3171 3.0878961E-10 1.0514874E-15 9.51
0.3415 2.4122E-10 1.0397619E-15 9.62
0.3659 1.8420436E-10 1.027253E-15 9.73
0.3902 1.3568625E-10 1.0134316E-15 9.87
0.4146 9.4098204E-11 9.9736509E-16 10.03
0.4390 5.8225517E-11 9.7705039E-16 10.23
0.4634 2.7113042E-11 9.4635835E-16 10.57
0.4878 3.9189098E-12 8.7666739E-16 11.41
0.5122 6.1E-13 8.1868768E-16 12.21
0.5366 3.1E-13 3.23E-02 12.51
0.5610 2.1E-13 4.76E-02 12.68
0.5854 1.6E-13 6.25E-02 12.80
0.6098 1.3E-13 7.69E-02 12.89
0.6341 1.1E-13 9.09E-02 12.96
0.6585 9.5714286E-14 1.04E-01 13.02
0.6829 8.5E-14 1.18E-01 13.07
0.7073 7.6666667E-14 1.30E-01 13.12
0.7317 7E-14 1.43E-01 13.15
0.7561 6.4545455E-14 1.55E-01 13.19
0.7805 6E-14 1.67E-01 13.22
0.8049 5.6153846E-14 1.78E-01 13.25
0.8293 5.2857143E-14 1.89E-01 13.28
0.8537 5E-14 2.00E-01 13.30
0.8780 4.75E-14 2.11E-01 13.32
0.9024 4.5294118E-14 2.21E-01 13.34
0.9268 4.3333333E-14 2.31E-01 13.36
0.9512 4.1578947E-14 2.41E-01 13.38
0.9756 4E-14 2.50E-01 13.40
00

00

00

00

00

00

00

00

00
0 10 20 30 40 50 60 70

Volumen de titulante NaOH/ml

eje y
PH SA a b c [H+]SA [OH-]SA αH2A
2.26 0.005510 1.81E-12 0.44902232
1.25 1 0.0544 - 0.006117 0.0556 1.80E-13 0.89160694
1.40 1 0.0977 - 0.005532 0.0401 2.49E-13 0.85584663
1.52 1 0.1372 - 0.004997 0.0299 3.34E-13 0.8156093
1.64 1 0.1734 - 0.004507 0.0230 4.36E-13 0.77245804
1.74 1 0.2068 - 0.004056 0.0180 5.54E-13 0.72744438
1.84 1 0.2375 - 0.003640 0.0144 6.92E-13 0.68121823
1.93 1 0.2660 - 0.003255 0.0117 8.53E-13 0.63417752
2.02 1 0.2925 - 0.002897 0.0096 1.04E-12 0.58657086
 2.10 1 0.3171 - 0.002564 0.0079 1.27E-12 0.53855844
2.19 1 0.3401 - 0.002254 0.0065 1.54E-12 0.49024679
2.27 1 0.3616 - 0.001963 0.0053 1.87E-12 0.44170896
2.36 1 0.3818 - 0.001690 0.0044 2.28E-12 0.39299642
2.45 1 0.4007 - 0.001434 0.0035 2.82E-12 0.34414639
2.55 1 0.4185 - 0.001193 0.0028 3.53E-12 0.29518633
2.66 1 0.4353 - 0.000966 0.0022 4.53E-12 0.24613696
2.78 1 0.4512 - 0.000751 0.0017 6.03E-12 0.19701412
2.93 1 0.4662 - 0.000548 0.0012 8.53E-12 0.14783017
3.13 1 0.4804 - 0.000356 0.0007 1.35E-11 0.09859487
3.46 1 0.4939 - 0.000173 0.0004 2.85E-11 0.04931601
5.65 2.2237673E-06 4.50E-09 0.0003287
7.85 1.4084895E-08 7.10E-07 1.97914E-06
8.19 6.51294E-09 1.54E-06 8.64891E-07
8.40 4.0053739E-09 2.50E-06 4.99914E-07
8.56 2.7630654E-09 3.62E-06 3.22234E-07
8.69 2.026248E-09 4.94E-06 2.19426E-07
8.81 1.5417104E-09 6.49E-06 1.53991E-07
8.92 1.2009677E-09 8.33E-06 1.09838E-07
9.02 9.4980375E-10 1.05E-05 7.89032E-08
9.12 7.5812E-10 1.32E-05 5.66956E-08
9.22 6.078744E-10 1.65E-05 4.05094E-08
9.31 4.8759978E-10 2.05E-05 2.86159E-08
9.41 3.8966308E-10 2.57E-05 1.98576E-08
9.51 3.0878961E-10 3.24E-05 1.34306E-08
9.62 2.4122E-10 4.15E-05 8.75953E-09
9.73 1.8420436E-10 5.43E-05 5.42271E-09
9.87 1.3568625E-10 7.37E-05 3.10509E-09
10.03 9.4098204E-11 1.06E-04 1.5677E-09
10.23 5.8225517E-11 1.72E-04 6.27175E-10
10.57 2.7113042E-11 3.69E-04 1.415E-10
11.41 1 1.35E-05 -6.58030674E-06 3.92E-12 2.55E-03 3.04818E-12
12.21 6.10E-13 2E-02 7.41827E-14
12.51 3.10E-13 3E-02 1.91665E-14
12.68 2.10E-13 5E-02 8.79661E-15
12.80 1.60E-13 6E-02 5.10677E-15
12.89 1.30E-13 8E-02 3.3714E-15
12.96 1.10E-13 9E-02 2.41391E-15
13.02 9.57E-14 1E-01 1.82767E-15
13.07 8.50E-14 1E-01 1.44141E-15
13.12 7.67E-14 1E-01 1.17265E-15
13.15 7.00E-14 1E-01 9.77586E-16
13.19 6.45E-14 2E-01 8.31177E-16
13.22 6.00E-14 2E-01 7.18236E-16
13.25 5.62E-14 2E-01 6.29109E-16
13.28 5.29E-14 2E-01 5.57412E-16
13.30 5.00E-14 2E-01 4.98782E-16
13.32 4.75E-14 2E-01 4.50152E-16
13.34 4.53E-14 2E-01 4.09314E-16
13.36 4.33E-14 2E-01 3.74644E-16
13.38 4.16E-14 2E-01 3.44923E-16
13.40 4.00E-14 3E-01 3.19225E-16
60 70

αHA- αA-2
81.49556 7.4131E-08
16.0325088 1.44487E-09
21.3218455 2.66228E-09
27.2733828 4.57084E-09
33.6559203 7.34933E-09
40.3139292 1.11972E-08
47.151276 1.63569E-08
54.1091069 2.31382E-08
61.1506456 3.19508E-08
68.2522011 4.33511E-08
75.3980156 5.81173E-08
82.5772848 7.73722E-08
89.782393 1.028E-07
97.0078374 1.37047E-07
104.249553 1.84524E-07
111.504478 2.53167E-07
118.770262 3.58854E-07
126.045074 5.38628E-07
133.327455 9.03618E-07
140.616169 2.00947E-06
147.812946 0.00033314
140.515019 0.0499999
132.795748 0.10218969
124.8109 0.15617426
116.62197 0.21153839
108.29184 0.26785708
99.883304 0.32470583
91.45762 0.38167049
83.0732043 0.43835613
74.7845118 0.49439459
66.6411445 0.54945053
58.6872021 0.60322579
50.9608762 0.65546217
43.4942727 0.70594261
36.3134392 0.75449101
29.4385648 0.80097087
22.8843184 0.84528302
16.6602868 0.88736264
10.771482 0.92717584
5.21888502 0.96471601
0.77781342 0.99474134
0.12161093 0.99917781
0.06182728 0.999582
0.04188864 0.9997168
0.03191731 0.99978421
0.02593386 0.99982467
0.02194463 0.99985164
0.01909505 0.9998709
0.01695779 0.99988535
0.01529543 0.99989659
0.01396552 0.99990558
0.01287739 0.99991294
0.01197061 0.99991907
0.01120332 0.99992426
0.01054563 0.9999287
0.00997564 0.99993256
0.00947689 0.99993593
0.00903681 0.9999389
0.00864563 0.99994155
0.00829563 0.99994391
0.00798062 0.99994604
 calculo de ph de hidroxilamina

titulante NaOH 0.01

Analito Glutamina 0.01

Va=
20 ml
Vb=
20 ml
Ca=
0.01 M
Cb=
0.01 M

K1 K2
9.33.E-07 1.0715E-08

Ve1=
20 ml
Ve2=
40 ml
Kw 1.00E-14
eje x
Vb/ml [H2A] [HA-]
zona I 0 0.01 0.0001
1 0.0090 0.0005
2 0.0082 0.0009
3 0.0074 0.0013
4 0.0067 0.0017
5 0.0060 0.0020
6 0.0054 0.0023
7 0.0048 0.0026
8 0.0043 0.0029
 9 0.0038 0.0031
10 0.0033 0.0033
zona II 11 0.0029 0.0035
12 0.0025 0.0038
13 0.0021 0.0039
14 0.0018 0.0041
15 0.0014 0.0043
16 0.0011 0.0044
17 0.0008 0.0046
18 0.0005 0.0047
19 0.0003 0.0049
zona III 20 0.0000 0.0050
21 0.0046
22 0.0043
23 0.0040
24 0.0036
25 0.0033
26 0.0030
27 0.0028
28 0.0025
zona IV 29 0.0022
30 0.0020
31 0.0018
32 0.0015
33 0.0013
34 0.0011
35 0.0009
36 0.0007
37 0.0005
38 0.0003
39 0.0002
zona V 40 0.0000
41
42
43
44
45
46
47
48
zona 49
50
51
52
53
54
55
56
57
58
59
60
de hidroxilamina

M Bureta
M Erlemmeyer

12.00

10.00

8.00

PH SA
6.00

4.00

2.00

9.332543E-09 kb1 CHA/Ka 10715.19305 0.00

0 10
Pka1 6.03
pka2 7.97 Kb1 9.33E-07

[A2-] [H+] CA [OH-]CA PH CA PH CA2 [H+]CA2 [OH-]CA2

0 0.00010 1.04E-10 4.02 4.02 0.0001 1.04E-10
0.00002 5.64E-10 4.75 4.04 0.0001 1.09E-10
0.00001 1.19E-09 5.08 4.06 0.0001 1.14E-10
0.00001 1.89E-09 5.28 4.08 0.0001 1.20E-10
0.00000 2.68E-09 5.43 4.10 0.0001 1.27E-10
0.00000 3.57E-09 5.55 4.13 0.0001 1.34E-10
0.00000 4.59E-09 5.66 4.15 0.0001 1.41E-10
0.00000 5.77E-09 5.76 4.17 0.0001 1.49E-10
0.00000 7.14E-09 5.85 4.20 0.0001 1.58E-10
 0.00000 8.77E-09 5.94 4.23 0.0001 1.68E-10
0.00000 1.07E-08 6.03 4.25 0.0001 1.79E-10
0.00000 1.31E-08 6.12 4.28 0.0001 1.92E-10
0.00000 1.61E-08 6.21 4.32 0.0000 2.07E-10
0.00000 1.99E-08 6.30 4.35 0.0000 2.25E-10
0.00000 2.50E-08 6.40 4.39 0.0000 2.46E-10
0.00000 3.21E-08 6.51 4.44 0.0000 2.74E-10
0.00000 4.29E-08 6.63 4.49 0.0000 3.11E-10
0.00000 6.07E-08 6.78 4.56 0.0000 3.64E-10
0.00000 9.64E-08 6.98 4.65 0.0000 4.51E-10
0.00000 2.04E-07 7.31 4.81 0.0000 6.46E-10
1E-06 7.07E-09 5.85 7.00 0.0000001 0.0000001
0.0002 2.0358867E-07 1.4944899E-15 6.69
0.0005 9.4140625E-08 1.4232398E-15 7.03
0.0007 5.7895268E-08 1.3817199E-15 7.24
0.0010 3.9938447E-08 1.3516054E-15 7.40
0.0012 2.9288194E-08 1.3274382E-15 7.53
0.0015 2.2284496E-08 1.3068484E-15 7.65
0.0017 1.7359264E-08 1.288582E-15 7.76
0.0020 1.3728841E-08 1.2718818E-15 7.86
0.0022 1.0958168E-08 1.2562401E-15 7.96
0.0024 8.7864583E-09 1.2412821E-15 8.06
0.0027 7.0479612E-09 1.2267024E-15 8.15
0.0029 5.6323451E-09 1.2122225E-15 8.25
0.0032 4.4633678E-09 1.1975564E-15 8.35
0.0034 3.4866898E-09 1.1823704E-15 8.46
0.0037 2.6625631E-09 1.1662215E-15 8.57
0.0039 1.961263E-09 1.14844E-15 8.71
0.0041 1.3601329E-09 1.1278511E-15 8.87
0.0044 8.4161478E-10 1.1019421E-15 9.07
0.0046 3.9190269E-10 1.0630583E-15 9.41
0.0049 1.5028491E-10 1.0180102E-15 9.82
0.0051 6.1E-11 9.7898413E-16 10.21
0.0054 3.1E-11 3.23E-04 10.51
0.0056 2.1E-11 4.76E-04 10.68
0.0059 1.6E-11 6.25E-04 10.80
0.0061 1.3E-11 7.69E-04 10.89
0.0063 1.1E-11 9.09E-04 10.96
0.0066 9.5714286E-12 1.04E-03 11.02
0.0068 8.5E-12 1.18E-03 11.07
0.0071 7.6666667E-12 1.30E-03 11.12
0.0073 7E-12 1.43E-03 11.15
0.0076 6.4545455E-12 1.55E-03 11.19
0.0078 6E-12 1.67E-03 11.22
0.0080 5.6153846E-12 1.78E-03 11.25
0.0083 5.2857143E-12 1.89E-03 11.28
0.0085 5E-12 2.00E-03 11.30
0.0088 4.75E-12 2.11E-03 11.32
0.0090 4.5294118E-12 2.21E-03 11.34
0.0093 4.3333333E-12 2.31E-03 11.36
0.0095 4.1578947E-12 2.41E-03 11.38
0.0098 4E-12 2.50E-03 11.40
 eje y eje y
12.00 1.2

1
10.00
0.8
8.00
0.6
PH SA

6.00
0.4
4.00
0.2
2.00
0
0.00 3.00 4.00 5.00 6.00 7.00 8
0 10 20 30 40 50 60 70
αH2A
Volumen de titulante NaOH/ml

eje y
PH SA a b c [H+]SA [OH-]SA αH2A
4.02 0.000096 1.04E-10 0.99038498
4.77 1 0.0005 - 0.000000 0.0000 5.85E-10 0.94817524
5.08 1 0.0009 - 0.000000 0.0000 1.20E-09 0.89896847
5.28 1 0.0013 - 0.000000 0.0000 1.90E-09 0.84913436
5.43 1 0.0017 - 0.000000 0.0000 2.69E-09 0.79909224
5.55 1 0.0020 - 0.000000 0.0000 3.58E-09 0.74893177
5.66 1 0.0023 - 0.000000 0.0000 4.60E-09 0.69868354
5.76 1 0.0026 - 0.000000 0.0000 5.78E-09 0.64836094
5.85 1 0.0029 - 0.000000 0.0000 7.15E-09 0.59797095
 5.94 1 0.0031 - 0.000000 0.0000 8.77E-09 0.5475178
6.03 1 0.0033 - 0.000000 0.0000 1.07E-08 0.49700451
6.12 1 0.0035 - 0.000000 0.0000 1.31E-08 0.44643367
6.21 1 0.0038 - 0.000000 0.0000 1.61E-08 0.39580808
6.30 1 0.0039 - 0.000000 0.0000 1.99E-08 0.34513129
6.40 1 0.0041 - 0.000000 0.0000 2.50E-08 0.29440861
6.51 1 0.0043 - 0.000000 0.0000 3.22E-08 0.243649
6.63 1 0.0044 - 0.000000 0.0000 4.29E-08 0.19286958
6.78 1 0.0046 - 0.000000 0.0000 6.07E-08 0.1421084
6.98 1 0.0047 - 0.000000 0.0000 9.65E-08 0.09147138
7.31 1 0.0049 - 0.000000 0.0000 2.04E-07 0.04140729
7.00 0.0000001 1.00E-07 0.08824145
6.69 2.0358867E-07 4.91E-08 0.17166548
7.03 9.4140625E-08 1.06E-07 0.08304433
7.24 5.7895268E-08 1.73E-07 0.04974353
7.40 3.9938447E-08 2.50E-07 0.0326407
7.53 2.9288194E-08 3.41E-07 0.02246067
7.65 2.2284496E-08 4.49E-07 0.01586897
7.76 1.7359264E-08 5.76E-07 0.01137064
7.86 1.3728841E-08 7.28E-07 0.00819448
7.96 1.0958168E-08 9.13E-07 0.00590172
8.06 8.7864583E-09 1.14E-06 0.00422394
8.15 7.0479612E-09 1.42E-06 0.0029875
8.25 5.6323451E-09 1.78E-06 0.00207503
8.35 4.4633678E-09 2.24E-06 0.00140438
8.46 3.4866898E-09 2.87E-06 0.00091639
8.57 2.6625631E-09 3.76E-06 0.00056751
8.71 1.961263E-09 5.10E-06 0.00032504
8.87 1.3601329E-09 7.35E-06 0.00016413
9.07 8.4161478E-10 1.19E-05 6.5669E-05
9.41 3.9190269E-10 2.55E-05 1.48166E-05
9.82 1 9.33E-07 -4.55246E-09 1.50E-10 6.65E-05 2.22731E-06
10.21 6.10E-11 2E-04 3.69994E-07
10.51 3.10E-11 3E-04 9.58228E-08
10.68 2.10E-11 5E-04 4.40137E-08
10.80 1.60E-11 6E-04 2.55618E-08
10.89 1.30E-11 8E-04 1.68795E-08
10.96 1.10E-11 9E-04 1.20876E-08
11.02 9.57E-12 1E-03 9.15305E-09
11.07 8.50E-12 1E-03 7.21927E-09
11.12 7.67E-12 1E-03 5.87358E-09
11.15 7.00E-12 1E-03 4.8968E-09
11.19 6.45E-12 2E-03 4.16361E-09
11.22 6.00E-12 2E-03 3.59799E-09
11.25 5.62E-12 2E-03 3.1516E-09
11.28 5.29E-12 2E-03 2.7925E-09
11.30 5.00E-12 2E-03 2.49883E-09
11.32 4.75E-12 2E-03 2.25525E-09
11.34 4.53E-12 2E-03 2.05069E-09
11.36 4.33E-12 2E-03 1.87702E-09
11.38 4.16E-12 2E-03 1.72814E-09
11.40 4.00E-12 3E-03 1.5994E-09
 eje y

4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00

αH2A

αHA- αA-2
10301.5314 1.07152E-06
55496.426 3.24819E-05
108117.9 0.00013003
161327.018 0.00030651
214658.673 0.00057663
267996.874 0.000959
321283.353 0.00147739
374470.549 0.00216281
427506.925 0.00305637
480328.201 0.00421384
532848.043 0.00571277
584944.559 0.0076643
636437.831 0.01023357
687049.274 0.01367702
736322.157 0.01841557
783451.198 0.0251918
826870.871 0.03544963
863080.237 0.05241826
882313.203 0.08510603
843175.463 0.17169558
882414.476 0.08824145
843197.61 0.04141673
882130.598 0.0937035
859198.607 0.14840567
817275.205 0.2046337
766884.695 0.26184089
712108.046 0.31955313
655018.323 0.3773307
596880.674 0.43476406
538568.573 0.49147684
480733.231 0.5471297
423881.07 0.60142367
368412.864 0.65410208
314645.653 0.70495121
262826.54 0.75379961
213142.523 0.80051632
165728.347 0.84500827
120673.299 0.88721699
78027.3651 0.92711496
37806.9196 0.96470171
14820.5929 0.98616639
6065.46794 0.99433901
3091.057 0.99711516
2095.89231 0.99804396
1597.61439 0.99850899
1298.42469 0.99878822
1098.87191 0.99897446
956.288636 0.99910753
849.326252 0.99920736
766.118508 0.99928501
699.543001 0.99934714
645.065972 0.99939799
599.664214 0.99944036
561.244335 0.99947621
528.310816 0.99950695
499.766794 0.99953359
474.789527 0.9995569
452.749794 0.99957747
433.158159 0.99959575
415.628194 0.99961211
399.850734 0.99962684
 calculo de ph de aspragina

titulante NaOH 0.01

Analito Glutamina 0.01

Va=
20 ml
Vb=
20 ml
Ca=
0.01 M
Cb=
0.01 M

K1 K2
0.0069 1.8621E-09

Ve1=
20 ml
Ve2=
40 ml
Kw 1.00E-14
eje x
Vb/ml [H2A] [HA-]
zona I 0 0.01 0.0055
1 0.0090 0.0005
2 0.0082 0.0009
3 0.0074 0.0013
4 0.0067 0.0017
5 0.0060 0.0020
6 0.0054 0.0023
7 0.0048 0.0026
8 0.0043 0.0029
 9 0.0038 0.0031
10 0.0033 0.0033
zona II 11 0.0029 0.0035
12 0.0025 0.0038
13 0.0021 0.0039
14 0.0018 0.0041
15 0.0014 0.0043
16 0.0011 0.0044
17 0.0008 0.0046
18 0.0005 0.0047
19 0.0003 0.0049
zona III 20 0.0000 0.0050
21 0.0046
22 0.0043
23 0.0040
24 0.0036
25 0.0033
26 0.0030
27 0.0028
28 0.0025
zona IV 29 0.0022
30 0.0020
31 0.0018
32 0.0015
33 0.0013
34 0.0011
35 0.0009
36 0.0007
37 0.0005
38 0.0003
39 0.0002
zona V 40 0.0000
41
42
43
44
45
46
47
48
zona 49
50
51
52
53
54
55
56
57
58
59
60
e aspragina

M Bureta
M Erlemmeyer

12.00

10.00

8.00

PH SA
6.00

4.00

2.00

6.91831E-05 kb1 CHA/Ka 1.445439771 0.00

0 10
Pka1 2.16
pka2 8.73 Kb1 5.37E-06

[A2-] [H+] CA [OH-]CA PH CA PH CA2 [H+]CA2 [OH-]CA2

0 0.00555 1.80E-12 2.26 2.08 0.0083 1.20E-12
0.13145 7.61E-14 0.88 2.10 0.0079 1.26E-12
0.06226 1.61E-13 1.21 2.12 0.0075 1.33E-12
0.03920 2.55E-13 1.41 2.15 0.0072 1.40E-12
0.02767 3.61E-13 1.56 2.17 0.0068 1.47E-12
0.02075 4.82E-13 1.68 2.19 0.0064 1.55E-12
0.01614 6.19E-13 1.79 2.21 0.0061 1.64E-12
0.01285 7.78E-13 1.89 2.24 0.0058 1.73E-12
0.01038 9.64E-13 1.98 2.26 0.0054 1.84E-12
 0.00846 1.18E-12 2.07 2.29 0.0051 1.95E-12
0.00692 1.45E-12 2.16 2.32 0.0048 2.08E-12
0.00566 1.77E-12 2.25 2.35 0.0045 2.23E-12
0.00461 2.17E-12 2.34 2.38 0.0042 2.40E-12
0.00373 2.68E-12 2.43 2.42 0.0038 2.61E-12
0.00296 3.37E-12 2.53 2.46 0.0035 2.86E-12
0.00231 4.34E-12 2.64 2.50 0.0031 3.18E-12
0.00173 5.78E-12 2.76 2.56 0.0028 3.61E-12
0.00122 8.19E-12 2.91 2.63 0.0024 4.22E-12
0.00077 1.30E-11 3.11 2.72 0.0019 5.24E-12
0.00036 2.75E-11 3.44 2.88 0.0013 7.51E-12
2E-05 4.70E-10 4.67 5.45 3.58921935E-06 2.78612E-09
0.0002 3.5379656E-08 1.3420574E-15 7.45
0.0005 1.6359766E-08 1.2843198E-15 7.79
0.0007 1.0061044E-08 1.2504131E-15 8.00
0.0010 6.9405066E-09 1.2256992E-15 8.16
0.0012 5.0897048E-09 1.2057916E-15 8.29
0.0015 3.8726015E-09 1.1887783E-15 8.41
0.0017 3.0166944E-09 1.1736444E-15 8.52
0.0020 2.3857991E-09 1.1597745E-15 8.62
0.0022 1.9043113E-09 1.1467545E-15 8.72
0.0024 1.5269115E-09 1.1342773E-15 8.82
0.0027 1.2247953E-09 1.1220906E-15 8.91
0.0029 9.7878939E-10 1.1099628E-15 9.01
0.0032 7.7564442E-10 1.0976542E-15 9.11
0.0034 6.0591724E-10 1.0848827E-15 9.22
0.0037 4.6270044E-10 1.0712717E-15 9.33
0.0039 3.4082845E-10 1.056249E-15 9.47
0.0041 2.36364E-10 1.0388079E-15 9.63
0.0044 1.4625589E-10 1.0167885E-15 9.83
0.0046 6.8104882E-11 9.8359155E-16 10.17
0.0049 6.3895389E-11 9.8091815E-16 10.19
0.0051 6.1E-11 9.7898413E-16 10.21
0.0054 3.1E-11 3.23E-04 10.51
0.0056 2.1E-11 4.76E-04 10.68
0.0059 1.6E-11 6.25E-04 10.80
0.0061 1.3E-11 7.69E-04 10.89
0.0063 1.1E-11 9.09E-04 10.96
0.0066 9.5714286E-12 1.04E-03 11.02
0.0068 8.5E-12 1.18E-03 11.07
0.0071 7.6666667E-12 1.30E-03 11.12
0.0073 7E-12 1.43E-03 11.15
0.0076 6.4545455E-12 1.55E-03 11.19
0.0078 6E-12 1.67E-03 11.22
0.0080 5.6153846E-12 1.78E-03 11.25
0.0083 5.2857143E-12 1.89E-03 11.28
0.0085 5E-12 2.00E-03 11.30
0.0088 4.75E-12 2.11E-03 11.32
0.0090 4.5294118E-12 2.21E-03 11.34
0.0093 4.3333333E-12 2.31E-03 11.36
0.0095 4.1578947E-12 2.41E-03 11.38
0.0098 4E-12 2.50E-03 11.40
 eje y eje y
12.00 0.5
0.45
10.00 0.4
0.35
8.00 0.3
0.25
PH SA

6.00
0.2
4.00 0.15
0.1
2.00 0.05
0
0.00 0.00 2.00 4.00 6.00
0 10 20 30 40 50 60 70
αH2A
Volumen de titulante NaOH/ml

eje y
PH SA a b c [H+]SA [OH-]SA αH2A
2.26 0.005549 1.80E-12 0.44508884
2.30 1 0.0074 - 0.000063 0.0050 1.99E-12 0.42125459
2.34 1 0.0078 - 0.000057 0.0046 2.19E-12 0.39763558
2.38 1 0.0082 - 0.000051 0.0041 2.42E-12 0.37422099
2.43 1 0.0086 - 0.000046 0.0037 2.67E-12 0.35100082
2.47 1 0.0089 - 0.000042 0.0034 2.96E-12 0.32796583
2.52 1 0.0092 - 0.000037 0.0030 3.29E-12 0.30510746
2.56 1 0.0095 - 0.000033 0.0027 3.67E-12 0.28241775
2.61 1 0.0098 - 0.000030 0.0024 4.12E-12 0.25988931
 2.67 1 0.0100 - 0.000026 0.0022 4.64E-12 0.23751522
2.72 1 0.0103 - 0.000023 0.0019 5.27E-12 0.21528905
2.78 1 0.0105 - 0.000020 0.0017 6.04E-12 0.19320476
2.84 1 0.0107 - 0.000017 0.0014 6.99E-12 0.17125669
2.92 1 0.0109 - 0.000015 0.0012 8.23E-12 0.14943953
2.99 1 0.0110 - 0.000012 0.0010 9.87E-12 0.1277483
3.09 1 0.0112 - 0.000010 0.0008 1.22E-11 0.10617828
3.19 1 0.0114 - 0.000008 0.0006 1.56E-11 0.08472506
3.33 1 0.0115 - 0.000006 0.0005 2.14E-11 0.06338444
3.52 1 0.0117 - 0.000004 0.0003 3.28E-11 0.04215247
3.83 1 0.0118 - 0.000002 0.0001 6.73E-11 0.02102542
5.63 2.3260057E-06 4.30E-09 0.00033583
7.45 3.5379656E-08 2.83E-07 4.8582E-06
7.79 1.6359766E-08 6.11E-07 2.12305E-06
8.00 1.0061044E-08 9.94E-07 1.22714E-06
8.16 6.9405066E-09 1.44E-06 7.90991E-07
8.29 5.0897048E-09 1.96E-06 5.38627E-07
8.41 3.8726015E-09 2.58E-06 3.78003E-07
8.52 3.0166944E-09 3.31E-06 2.69619E-07
8.62 2.3857991E-09 4.19E-06 1.93684E-07
8.72 1.9043113E-09 5.25E-06 1.39171E-07
8.82 1.5269115E-09 6.55E-06 9.94389E-08
8.91 1.2247953E-09 8.16E-06 7.02436E-08
9.01 9.7878939E-10 1.02E-05 4.87446E-08
9.11 7.7564442E-10 1.29E-05 3.29682E-08
9.22 6.0591724E-10 1.65E-05 2.15021E-08
9.33 4.6270044E-10 2.16E-05 1.33112E-08
9.47 3.4082845E-10 2.93E-05 7.62209E-09
9.63 2.36364E-10 4.23E-05 3.84825E-09
9.83 1.4625589E-10 6.84E-05 1.53953E-09
10.17 6.8104882E-11 1.47E-04 3.47341E-10
10.19 1 5.37E-06 -2.6196673E-08 6.39E-11 1.57E-04 3.06399E-10
10.21 6.10E-11 2E-04 2.7968E-10
10.51 3.10E-11 3E-04 7.33758E-11
10.68 2.10E-11 5E-04 3.38507E-11
10.80 1.60E-11 6E-04 1.97026E-11
10.89 1.30E-11 8E-04 1.30276E-11
10.96 1.10E-11 9E-04 9.33743E-12
11.02 9.57E-12 1E-03 7.07501E-12
11.07 8.50E-12 1E-03 5.5829E-12
11.12 7.67E-12 1E-03 4.5439E-12
11.15 7.00E-12 1E-03 3.78937E-12
11.19 6.45E-12 2E-03 3.22276E-12
11.22 6.00E-12 2E-03 2.78551E-12
11.25 5.62E-12 2E-03 2.44035E-12
11.28 5.29E-12 2E-03 2.1626E-12
11.30 5.00E-12 2E-03 1.93542E-12
11.32 4.75E-12 2E-03 1.74695E-12
11.34 4.53E-12 2E-03 1.58865E-12
11.36 4.33E-12 2E-03 1.45424E-12
11.38 4.16E-12 2E-03 1.33899E-12
11.40 4.00E-12 3E-03 1.23933E-12
 eje y

2.00 4.00 6.00 8.00 10.00 12.00

αH2A

αHA- αA-2
80.2090389 1.86209E-07
83.6541329 2.14008E-07
87.0681134 2.45603E-07
90.4525464 2.81652E-07
93.8088763 3.22983E-07
97.1384383 3.70641E-07
100.44247 4.25973E-07
103.722121 4.90739E-07
106.978461 5.67289E-07
110.212487 6.58825E-07
113.42513 7.69833E-07
116.617262 9.06792E-07
119.789698 1.07942E-06
122.943204 1.303E-06
126.078499 1.60298E-06
129.196253 2.02518E-06
132.297096 2.66127E-06
135.3816 3.72509E-06
138.450244 5.8582E-06
141.503106 1.22684E-05
144.379852 0.00079964
137.316111 0.04999976
129.772784 0.10218956
121.969768 0.15617414
113.967276 0.21153829
105.826783 0.267857
97.6096606 0.32470576
89.3757761 0.38167043
81.182218 0.43835608
73.0822025 0.49439455
65.124203 0.54945049
57.3513159 0.60322576
49.8008636 0.65546215
42.5042214 0.7059426
35.4868439 0.754491
28.7684611 0.80097086
22.3634076 0.84528301
16.2810523 0.88736263
10.5262932 0.92717584
5.10008869 0.96471601
4.79531536 0.96682452
4.5849106 0.96828017
2.36696093 0.98362463
1.61194002 0.9888481
1.23141445 0.99148069
1.002125 0.99306699
0.84885733 0.99412734
0.73917988 0.99488612
0.65681185 0.99545597
0.59268257 0.99589964
0.54133797 0.99625486
0.4993015 0.99654568
0.46425236 0.99678816
0.43458207 0.99699343
0.40914066 0.99716944
0.38708418 0.99732203
0.36777921 0.99745559
0.35074113 0.99757346
0.33559278 0.99767827
0.3220363 0.99777205
0.30983329 0.99785648
 curva de valoracion

12.00

10.00

8.00
PH SA

6.00

4.00

2.00

0.00
0 10 20 30 40 50 60

Volumen de titulante NaOH/ml

 alto.

asparagina
glutamina
hidroxilamina

0 50 60 70

OH/ml
 titulante NaOH
Analito Glutamina

Va=
20 ml
Vb=
20 ml
Ca=
0.01 M
Cb=
0.01 M

K1 K2
0.0068 7.4131E-10

Ve1=
20 ml
Ve2=
40 ml
Kw 1.00E-14
eje x
Vb/ml promedio promedio 2 [H2A]
zona I 0 0.01
1 0.5 0.0090
2 1.5 1.0 0.0082
3 2.5 2.0 0.0074
4 3.5 3.0 0.0067
5 4.5 4.0 0.0060
6 5.5 5.0 0.0054
7 6.5 6.0 0.0048
8 7.5 7.0 0.0043
 9 8.5 8.0 0.0038
10 9.5 9.0 0.0033
zona II 11 10.5 10.0 0.0029
12 11.5 11.0 0.0025

13 12.5 12.0 0.0021

14 13.5 13.0 0.0018
15 14.5 14.0 0.0014
16 15.5 15.0 0.0011
16.3 16.15 15.8 0.0010
16.6 16.45 16.3 0.0009
16.9 16.75 16.6 0.0008
17.3 17.1 16.9 0.0007
17.6 17.45 17.3 0.0006
17.8 17.7 17.6 0.0006
18 17.9 17.8 0.0005
19 18.5 18.2 0.0003
zona III 20 19.5 19.0 0.0000
21 20.5 20.0
22 21.5 21.0
23 22.5 22.0
24 23.5 23.0
25 24.5 24.0
26 25.5 25.0
27 26.5 26.0
28 27.5 27.0
zona IV 29 28.5 28.0
30 29.5 29.0
31 30.5 30.0
32 31.5 31.0
32.3 32.15 31.8
32.7 32.5 32.3
33 32.85 32.7
33.3 33.15 33.0
33.6 33.45 33.3
34 33.8 33.6
34.3 34.15 34.0
34.8 34.55 34.4
35 34.9 34.7
35.4 35.2 35.1
35.8 35.6 35.4
36 35.55 35.3
37 36.5 36.0
38 37.5 37.0
39 38.5 38.0
zona V 40 39.5 39.0
41 40.5 40.0
42 41.5 41.0
43 42.5 42.0
44 43.5 43.0
45 44.5 44.0
46 45.5 45.0
47 46.5 46.0
48 47.5 47.0
zona 49 48.5 48.0
50 49.5 49.0
51 50.5 50.0
52 51.5 51.0
53 52.5 52.0
54 53.5 53.0
55 54.5 54.0
56 55.5 55.0
57 56.5 56.0
58 57.5 57.0
59 58.5 58.0
60 59.5 59.0
 0.01 M Bureta
0.01 M Erlemmeyer

6.76083E-05 kb1 CHA/Ka 1.479108388

Pka1 2.17
pka2 9.13 Kb1 1.35E-05

[HA-] [A2-] [H+] CA [OH-]CA PH CA PH CA2 [H+]CA2

0.0055 0 0.00551 1.81E-12 2.26 2.09 0.0082
0.0005 0.12846 7.78E-14 0.89 2.11 0.0078
0.0009 0.06085 1.64E-13 1.22 2.13 0.0074
0.0013 0.03831 2.61E-13 1.42 2.15 0.0071
0.0017 0.02704 3.70E-13 1.57 2.17 0.0067
0.0020 0.02028 4.93E-13 1.69 2.20 0.0064
0.0023 0.01578 6.34E-13 1.80 2.22 0.0060
0.0026 0.01256 7.96E-13 1.90 2.24 0.0057
0.0029 0.01014 9.86E-13 1.99 2.27 0.0054
0.0031 0.00826 1.21E-12 2.08 2.30 0.0051
0.0033 0.00676 1.48E-12 2.17 2.32 0.0047
0.0035 0.00553 1.81E-12 2.26 2.35 0.0044
0.0038 0.00451 2.22E-12 2.35 2.39 0.0041

0.0039 0.00364 2.75E-12 2.44 2.42 0.0038

0.0041 0.00290 3.45E-12 2.54 2.46 0.0035
0.0043 0.00225 4.44E-12 2.65 2.51 0.0031
0.0044 0.00169 5.92E-12 2.77 2.56 0.0027
0.0045 0.00153 6.52E-12 2.81 2.58 0.0026
0.0045 0.00138 7.22E-12 2.86 2.60 0.0025
0.0046 0.00124 8.06E-12 2.91 2.62 0.0024
0.0046 0.00106 9.48E-12 2.98 2.66 0.0022
0.0047 0.00092 1.08E-11 3.04 2.68 0.0021
0.0047 0.00084 1.20E-11 3.08 2.70 0.0020
0.0047 0.00075 1.33E-11 3.12 2.72 0.0019
0.0049 0.00036 2.81E-11 3.45 2.88 0.0013
0.0050 1E-05 7.43E-10 4.87 5.65 2.23872114E-06
0.0046 0.0002 1.4084895E-08 1.2736831E-15 7.85
0.0043 0.0005 6.51294E-09 1.2215646E-15 8.19
0.0040 0.0007 4.0053739E-09 1.1908509E-15 8.40
0.0036 0.0010 2.7630654E-09 1.1684142E-15 8.56
0.0033 0.0012 2.026248E-09 1.1503102E-15 8.69
0.0030 0.0015 1.5417104E-09 1.1348165E-15 8.81
0.0028 0.0017 1.2009677E-09 1.1210173E-15 8.92
0.0025 0.0020 9.4980375E-10 1.1083567E-15 9.02
0.0022 0.0022 7.5812E-10 1.0964597E-15 9.12
0.0020 0.0024 6.078744E-10 1.0850475E-15 9.22
0.0018 0.0027 4.8759978E-10 1.0738905E-15 9.31
0.0015 0.0029 3.8966308E-10 1.0627771E-15 9.41
0.0015 0.0030 3.6380426E-10 1.0594194E-15 9.44
0.0014 0.0031 3.3150675E-10 1.0549071E-15 9.48
0.0013 0.0032 3.0878961E-10 1.0514874E-15 9.51
0.0013 0.0032 2.8726308E-10 1.0480291E-15 9.54
0.0012 0.0033 2.6684565E-10 1.0445239E-15 9.57
0.0011 0.0034 2.4122E-10 1.0397619E-15 9.62
0.0010 0.0035 2.2311196E-10 1.0361108E-15 9.65
0.0009 0.0036 1.9486997E-10 1.0298391E-15 9.71
0.0009 0.0037 1.8420436E-10 1.027253E-15 9.73
0.0008 0.0038 1.6387443E-10 1.0219214E-15 9.79
0.0008 0.0039 1.447911E-10 1.0163368E-15 9.84
0.0007 0.0039 1.3568625E-10 1.0134316E-15 9.87
0.0005 0.0041 9.4098204E-11 9.9736509E-16 10.03
0.0003 0.0044 5.8225517E-11 9.7705039E-16 10.23
0.0002 0.0046 2.7113042E-11 9.4635835E-16 10.57
0.0000 0.0049 4.1131932E-11 9.6285119E-16 10.39
0.0051 6.1E-11 9.7898413E-16 10.21
0.0054 3.1E-11 3.23E-04 10.51
0.0056 2.1E-11 4.76E-04 10.68
0.0059 1.6E-11 6.25E-04 10.80
0.0061 1.3E-11 7.69E-04 10.89
0.0063 1.1E-11 9.09E-04 10.96
0.0066 9.5714286E-12 1.04E-03 11.02
0.0068 8.5E-12 1.18E-03 11.07
0.0071 7.6666667E-12 1.30E-03 11.12
0.0073 7E-12 1.43E-03 11.15
0.0076 6.4545455E-12 1.55E-03 11.19
0.0078 6E-12 1.67E-03 11.22
0.0080 5.6153846E-12 1.78E-03 11.25
0.0083 5.2857143E-12 1.89E-03 11.28
0.0085 5E-12 2.00E-03 11.30
0.0088 4.75E-12 2.11E-03 11.32
0.0090 4.5294118E-12 2.21E-03 11.34
0.0093 4.3333333E-12 2.31E-03 11.36
0.0095 4.1578947E-12 2.41E-03 11.38
0.0098 4E-12 2.50E-03 11.40
 14.00

12.00

10.00

8.00

6.00
PH SA

4.00

2.00

0.00
0 10 20 30 40

-2.00

-4.00

Volumen de titulante NaOH/ml

eje y
[OH-]CA2 PH SA a b c dpH/Dv d2pH/Dv2
1.22E-12 2.26
1.28E-12 2.30 1 0.0072 - 0.000061 0.042243
1.34E-12 2.34 1 0.0077 - 0.000055 0.042505 0.000262
1.41E-12 2.39 1 0.0081 - 0.000050 0.042990 0.000485
1.49E-12 2.43 1 0.0084 - 0.000045 0.043712 0.000722
1.57E-12 2.48 1 0.0088 - 0.000041 0.044693 0.000981
1.66E-12 2.52 1 0.0091 - 0.000036 0.045966 0.001273
1.75E-12 2.57 1 0.0094 - 0.000033 0.047576 0.001611
1.86E-12 2.62 1 0.0096 - 0.000029 0.049588 0.002012
 1.97E-12 2.67 1 0.0099 - 0.000026 0.052089 0.002501
2.11E-12 2.73 1 0.0101 - 0.000023 0.055204 0.003115
2.26E-12 2.78 1 0.0103 - 0.000020 0.059112 0.003908
2.43E-12 2.85 1 0.0105 - 0.000017 0.064079 0.004968

2.64E-12 2.92 1 0.0107 - 0.000014 0.070518 0.006439

2.90E-12 3.00 1 0.0109 - 0.000012 0.079097 0.008578
3.22E-12 3.09 1 0.0110 - 0.000010 0.090977 0.011880
3.65E-12 3.20 1 0.0112 - 0.000008 0.108371 0.017395
3.81E-12 3.23 1 0.0113 - 0.000007 0.124114 0.024220
3.99E-12 3.27 1 0.0113 - 0.000006 0.133570 0.031518
4.20E-12 3.32 1 0.0113 - 0.000006 0.144793 0.037411
4.52E-12 3.38 1 0.0114 - 0.000005 0.160959 0.046188
4.81E-12 3.44 1 0.0114 - 0.000004 0.181369 0.058316
5.04E-12 3.48 1 0.0115 - 0.000004 0.199732 0.073448
5.30E-12 3.52 1 0.0115 - 0.000004 0.217695 0.089817
7.60E-12 3.83 1 0.0116 - 0.000002 0.311594 0.156499
4.46684E-09 5.84 2.002947 1.691353
7.85 2.016097 0.013151
8.19 0.334977 - 1.681121
8.40 0.211134 - 0.123843
8.56 0.161252 - 0.049882
8.69 0.134699 - 0.026553
8.81 0.118690 - 0.016009
8.92 0.108471 - 0.010218
9.02 0.101897 - 0.006574
9.12 0.097896 - 0.004002
9.22 0.095924 - 0.001972
9.31 0.095750 - 0.000174
9.41 0.097374 0.001624
9.44 0.099405 0.003124
9.48 0.100939 0.004382
9.51 0.102766 0.005221
9.54 0.104610 0.006146
9.57 0.106732 0.007076
9.62 0.109617 0.008242
9.65 0.112968 0.009575
9.71 0.117556 0.011469
9.73 0.122225 0.013341
9.79 0.126972 0.015822
9.84 0.134423 0.018629
9.87 0.141030 - 0.132135
10.03 0.158955 0.018868
10.23 0.208468 0.049513
10.57 0.331935 0.123467
10.39 1 1.35E-05 -6.58030674E-08 - 0.181001 - 0.512936
10.21 - 0.171151 0.009850
10.51 0.293968 0.465119
10.68 0.169142 - 0.124826
10.80 0.118099 - 0.051043
10.89 0.090177 - 0.027923
10.96 0.072551 - 0.017626
11.02 0.060416 - 0.012135
11.07 0.051558 - 0.008858
11.12 0.044812 - 0.006745
11.15 0.039509 - 0.005304
11.19 0.035232 - 0.004276
11.22 0.031714 - 0.003518
11.25 0.028772 - 0.002943
11.28 0.026276 - 0.002496
11.30 0.024134 - 0.002142
11.32 0.022276 - 0.001857
11.34 0.020652 - 0.001625
11.36 0.019220 - 0.001432
11.38 0.017949 - 0.001271
11.40 0.016813 - 0.001135
 0.012000

0.010000 f(x) = − 0.00

R² = 0.99954

0.008000

0.006000

0.004000

30 40 50 60 70

0.002000

olumen de titulante NaOH/ml

-
15

Gramm [H+]SA [OH-]SA αH2A αHA- αA-2

0.005510 1.81E-12 0.44902232 81.49556 7.4131E-08
0.0050 2.00E-12 0.42509554 85.0345884 8.5252E-08
0.0045 2.21E-12 0.40136969 88.5438961 9.78977E-08
0.0041 2.44E-12 0.37783472 92.0249719 1.12334E-07
0.0037 2.69E-12 0.35448132 95.4791903 1.28891E-07
0.0033 2.99E-12 0.3313009 98.9078234 1.47992E-07
0.0030 3.32E-12 0.30828547 102.312051 1.70177E-07
0.0027 3.70E-12 0.28542763 105.692969 1.96154E-07
0.0024 4.15E-12 0.26272047 109.0516 2.26866E-07
 0.0021 4.68E-12 0.24015755 112.388896 2.63603E-07
0.0019 5.31E-12 0.21773284 115.705747 3.08167E-07
0.0016 6.09E-12 0.1954407 119.002987 3.63162E-07
0.0014 7.06E-12 0.17327585 122.281398 4.32497E-07

0.0012 8.30E-12 0.15123333 125.541713 5.2231E-07

0.0010 9.96E-12 0.12930845 128.784623 6.42837E-07
0.0008 1.23E-11 0.10749682 132.010774 8.125E-07
0.010152 0.0006 1.58E-11 0.08579429 135.220775 1.06814E-06
0.009492 0.0006 1.72E-11 0.07930421 136.180713 1.17202E-06
0.008815 0.0005 1.88E-11 0.0728235 137.139262 1.29435E-06
0.008120 0.0005 2.08E-11 0.06635205 138.096438 1.44049E-06
0.007167 0.0004 2.41E-11 0.05773769 139.37056 1.6861E-06
0.006433 0.0004 2.74E-11 0.05128747 140.324582 1.92423E-06
0.005934 0.0003 3.00E-11 0.04699229 140.959856 2.11916E-06
0.005428 0.0003 3.32E-11 0.04270106 141.594541 2.35318E-06
0.0001 6.80E-11 0.02130316 144.759142 4.93001E-06
1.4616759E-06 6.84E-09 0.00021604 147.803923 0.0005068
1.4084895E-08 7.10E-07 1.97914E-06 140.515019 0.0499999
6.51294E-09 1.54E-06 8.64891E-07 132.795748 0.10218969
4.0053739E-09 2.50E-06 4.99914E-07 124.8109 0.15617426
2.7630654E-09 3.62E-06 3.22234E-07 116.62197 0.21153839
2.026248E-09 4.94E-06 2.19426E-07 108.29184 0.26785708
1.5417104E-09 6.49E-06 1.53991E-07 99.883304 0.32470583
1.2009677E-09 8.33E-06 1.09838E-07 91.45762 0.38167049
9.4980375E-10 1.05E-05 7.89032E-08 83.0732043 0.43835613
7.5812E-10 1.32E-05 5.66956E-08 74.7845118 0.49439459
6.078744E-10 1.65E-05 4.05094E-08 66.6411445 0.54945053
4.8759978E-10 2.05E-05 2.86159E-08 58.6872021 0.60322579
1.246921842E-08 3.8966308E-10 2.57E-05 1.98576E-08 50.9608762 0.65546217
1.175087764E-08 3.6380426E-10 2.75E-05 1.77145E-08 48.6923232 0.67079948
1.084027085E-08 3.3150675E-10 3.02E-05 1.51516E-08 45.7053174 0.69099412
1.019005703E-08 3.0878961E-10 3.24E-05 1.34306E-08 43.4942727 0.70594261
9.565860494E-09 2.8726308E-10 3.48E-05 1.18665E-08 41.3089874 0.72071696
8.966013946E-09 2.6684565E-10 3.75E-05 1.0447E-08 39.1500602 0.7353131
8.201479971E-09 2.4122E-10 4.15E-05 8.75953E-09 36.3134392 0.75449101
7.652740121E-09 2.2311196E-10 4.48E-05 7.63446E-09 34.2180805 0.76865738
6.781475084E-09 1.9486997E-10 5.13E-05 5.99971E-09 30.7882827 0.79184566
6.447152705E-09 1.8420436E-10 5.43E-05 5.42271E-09 29.4385648 0.80097087
5.801154939E-09 1.6387443E-10 6.10E-05 4.38819E-09 26.7777454 0.81896022
5.183521305E-09 1.447911E-10 6.91E-05 3.49945E-09 24.168988 0.83659758
4.884704983E-09 1.3568625E-10 7.37E-05 3.10509E-09 22.8843184 0.84528302
9.4098204E-11 1.06E-04 1.5677E-09 16.6602868 0.88736264
5.8225517E-11 1.72E-04 6.27175E-10 10.771482 0.92717584
2.7113042E-11 3.69E-04 1.415E-10 5.21888502 0.96471601
4.11E-11 2.43E-04 3.1982E-10 7.77547374 0.94743135
6.10E-11 2E-04 6.85989E-10 11.245726 0.92396956
3.10E-11 3E-04 1.84048E-10 5.93703897 0.95986069
2.10E-11 5E-04 8.55671E-11 4.07462401 0.97245216
1.60E-11 6E-04 5E-11 3.12497216 0.9788726
1.30E-11 8E-04 3.31388E-11 2.54913801 0.98276571
1.10E-11 9E-04 2.37897E-11 2.16269717 0.98537837
9.57E-12 1E-03 1.8046E-11 1.88540763 0.98725308
8.50E-12 1E-03 1.42524E-11 1.67674708 0.9886638
7.67E-12 1E-03 1.16077E-11 1.5140428 0.98976381
7.00E-12 1E-03 9.68533E-12 1.38361847 0.99064559
6.45E-12 2E-03 8.24074E-12 1.27673468 0.99136821
6.00E-12 2E-03 7.12527E-12 1.18754566 0.99197121
5.62E-12 2E-03 6.24427E-12 1.11199324 0.992482
5.29E-12 2E-03 5.53505E-12 1.04717207 0.99292025
5.00E-12 2E-03 4.95474E-12 0.9909474 0.99330037
4.75E-12 2E-03 4.47315E-12 0.94171549 0.99363322
4.53E-12 2E-03 4.06854E-12 0.89824816 0.9939271
4.33E-12 2E-03 3.72489E-12 0.85958894 0.99418847
4.16E-12 2E-03 3.43019E-12 0.82498187 0.99442244
4.00E-12 3E-03 3.17529E-12 0.79382158 0.99463311
0

0 f(x) = − 0.00236509841130255 x + 0.0480470869037955 f(x) = − 1.87943986842111E-0

R² = 0.999546833143764 R² = 0.995143927267444

-
15 20 25 30 35

Gráfico de Gram Linear (Gráfico de Gram) Grafico de Gram Ve2 Linear (Grafico de G

Ve1=x Ve=x
0.048 2E-09
0.0024 0.00000007
y 0 y 0
x 20 ml x 35

pendiente 0.0023651 1.879439868E-09

 intercepto 0.04804709 7.229406441E-08
20 38

INDICADOR

6,0 - 7,6
PH MAS A PH MAS
ACIDO BASICO

AZUL DE BROMOTIMOL
 0.000000014

0.000000012
= − 1.87943986842111E-09 x + 7.22940644074018E-08
0.995143927267444

0.00000001

0.000000008

0.000000006

0.000000004

0.000000002

0
35 40

Ve2 Linear (Grafico de Gram Ve2)

ml
grafico de fraccion composicional vs tiempo.

titulante NaOH 0.01

Analito Glutamina 0.01

Va=
20 ml
Vb=
20 ml
Ca=
0.01 M
Cb=
0.01 M

K1 K2
0.0068 7.4131E-10

Ve1=
20 ml
Ve2=
40 ml
Kw 1.00E-14
eje x
Vb/ml [H2A] [HA-]
zona I 0 0.01 0.0055
1 0.0090 0.0005
2 0.0082 0.0009
3 0.0074 0.0013
4 0.0067 0.0017
5 0.0060 0.0020
6 0.0054 0.0023
7 0.0048 0.0026
8 0.0043 0.0029
 9 0.0038 0.0031
10 0.0033 0.0033
zona II 11 0.0029 0.0035
12 0.0025 0.0038
13 0.0021 0.0039
14 0.0018 0.0041
15 0.0014 0.0043
16 0.0011 0.0044
17 0.0008 0.0046
18 0.0005 0.0047
19 0.0003 0.0049
zona III 20 0.0000 0.0050
21 0.0046
22 0.0043
23 0.0040
24 0.0036
25 0.0033
26 0.0030
27 0.0028
28 0.0025
zona IV 29 0.0022
30 0.0020
31 0.0018
32 0.0015
33 0.0013
34 0.0011
35 0.0009
36 0.0007
37 0.0005
38 0.0003
39 0.0002
zona V 40 0.0000
41
42
43
44
45
46
47
48
zona 49
50
51
52
53
54
55
56
57
58
59
60
M Bureta
M Erlemmeyer

160

140

120

100

80

60

6.76083E-05 kb1 CHA/Ka 1.479108388 40

Pka1 2.17
20
pka2 9.13 Kb1 1.35E-05
0
0.00 2.

[A2-] [H+] CA [OH-]CA PH CA PH CA2 [H+]CA2 [OH-]CA2

0 0.00551 1.81E-12 2.26 2.09 0.0082 1.22E-12
0.12846 7.78E-14 0.89 2.11 0.0078 1.28E-12
0.06085 1.64E-13 1.22 2.13 0.0074 1.34E-12
0.03831 2.61E-13 1.42 2.15 0.0071 1.41E-12
0.02704 3.70E-13 1.57 2.17 0.0067 1.49E-12
0.02028 4.93E-13 1.69 2.20 0.0064 1.57E-12
0.01578 6.34E-13 1.80 2.22 0.0060 1.66E-12
0.01256 7.96E-13 1.90 2.24 0.0057 1.75E-12
0.01014 9.86E-13 1.99 2.27 0.0054 1.86E-12
 0.00826 1.21E-12 2.08 2.30 0.0051 1.97E-12
0.00676 1.48E-12 2.17 2.32 0.0047 2.11E-12
0.00553 1.81E-12 2.26 2.35 0.0044 2.26E-12
0.00451 2.22E-12 2.35 2.39 0.0041 2.43E-12
0.00364 2.75E-12 2.44 2.42 0.0038 2.64E-12
0.00290 3.45E-12 2.54 2.46 0.0035 2.90E-12
0.00225 4.44E-12 2.65 2.51 0.0031 3.22E-12
0.00169 5.92E-12 2.77 2.56 0.0027 3.65E-12
0.00119 8.38E-12 2.92 2.63 0.0023 4.27E-12
0.00075 1.33E-11 3.12 2.72 0.0019 5.30E-12
0.00036 2.81E-11 3.45 2.88 0.0013 7.60E-12
1E-05 7.43E-10 4.87 5.65 2.23872114E-06 4.46684E-09
0.0002 1.4084895E-08 1.2736831E-15 7.85
0.0005 6.51294E-09 1.2215646E-15 8.19
0.0007 4.0053739E-09 1.1908509E-15 8.40
0.0010 2.7630654E-09 1.1684142E-15 8.56
0.0012 2.026248E-09 1.1503102E-15 8.69
0.0015 1.5417104E-09 1.1348165E-15 8.81
0.0017 1.2009677E-09 1.1210173E-15 8.92
0.0020 9.4980375E-10 1.1083567E-15 9.02
0.0022 7.5812E-10 1.0964597E-15 9.12
0.0024 6.078744E-10 1.0850475E-15 9.22
0.0027 4.8759978E-10 1.0738905E-15 9.31
0.0029 3.8966308E-10 1.0627771E-15 9.41
0.0032 3.0878961E-10 1.0514874E-15 9.51
0.0034 2.4122E-10 1.0397619E-15 9.62
0.0037 1.8420436E-10 1.027253E-15 9.73
0.0039 1.3568625E-10 1.0134316E-15 9.87
0.0041 9.4098204E-11 9.9736509E-16 10.03
0.0044 5.8225517E-11 9.7705039E-16 10.23
0.0046 2.7113042E-11 9.4635835E-16 10.57
0.0049 4.1131932E-11 9.6285119E-16 10.39
0.0051 6.1E-11 9.7898413E-16 10.21
0.0054 3.1E-11 3.23E-04 10.51
0.0056 2.1E-11 4.76E-04 10.68
0.0059 1.6E-11 6.25E-04 10.80
0.0061 1.3E-11 7.69E-04 10.89
0.0063 1.1E-11 9.09E-04 10.96
0.0066 9.5714286E-12 1.04E-03 11.02
0.0068 8.5E-12 1.18E-03 11.07
0.0071 7.6666667E-12 1.30E-03 11.12
0.0073 7E-12 1.43E-03 11.15
0.0076 6.4545455E-12 1.55E-03 11.19
0.0078 6E-12 1.67E-03 11.22
0.0080 5.6153846E-12 1.78E-03 11.25
0.0083 5.2857143E-12 1.89E-03 11.28
0.0085 5E-12 2.00E-03 11.30
0.0088 4.75E-12 2.11E-03 11.32
0.0090 4.5294118E-12 2.21E-03 11.34
0.0093 4.3333333E-12 2.31E-03 11.36
0.0095 4.1578947E-12 2.41E-03 11.38
0.0098 4E-12 2.50E-03 11.40
160

140

120

100

80

60

40

20

0
0.00 2.00 4.00 6.00 8.00 10.00 12

αHA- αH2A αA-2

eje y
PH SA a b c [H+]SA [OH-]SA αH2A
2.26 0.005510 1.81E-12 0.44902232
2.30 1 0.0072 - 0.000061 0.0050 2.00E-12 0.42509554
2.34 1 0.0077 - 0.000055 0.0045 2.21E-12 0.40136969
2.39 1 0.0081 - 0.000050 0.0041 2.44E-12 0.37783472
2.43 1 0.0084 - 0.000045 0.0037 2.69E-12 0.35448132
2.48 1 0.0088 - 0.000041 0.0033 2.99E-12 0.3313009
2.52 1 0.0091 - 0.000036 0.0030 3.32E-12 0.30828547
2.57 1 0.0094 - 0.000033 0.0027 3.70E-12 0.28542763
2.62 1 0.0096 - 0.000029 0.0024 4.15E-12 0.26272047
 2.67 1 0.0099 - 0.000026 0.0021 4.68E-12 0.24015755
2.73 1 0.0101 - 0.000023 0.0019 5.31E-12 0.21773284
2.78 1 0.0103 - 0.000020 0.0016 6.09E-12 0.1954407
2.85 1 0.0105 - 0.000017 0.0014 7.06E-12 0.17327585
2.92 1 0.0107 - 0.000014 0.0012 8.30E-12 0.15123333
3.00 1 0.0109 - 0.000012 0.0010 9.96E-12 0.12930845
3.09 1 0.0110 - 0.000010 0.0008 1.23E-11 0.10749682
3.20 1 0.0112 - 0.000008 0.0006 1.58E-11 0.08579429
3.33 1 0.0114 - 0.000005 0.0005 2.16E-11 0.06419694
3.52 1 0.0115 - 0.000004 0.0003 3.32E-11 0.04270106
3.83 1 0.0116 - 0.000002 0.0001 6.80E-11 0.02130316
5.84 1.4616759E-06 6.84E-09 0.00021604
7.85 1.4084895E-08 7.10E-07 1.97914E-06
8.19 6.51294E-09 1.54E-06 8.64891E-07
8.40 4.0053739E-09 2.50E-06 4.99914E-07
8.56 2.7630654E-09 3.62E-06 3.22234E-07
8.69 2.026248E-09 4.94E-06 2.19426E-07
8.81 1.5417104E-09 6.49E-06 1.53991E-07
8.92 1.2009677E-09 8.33E-06 1.09838E-07
9.02 9.4980375E-10 1.05E-05 7.89032E-08
9.12 7.5812E-10 1.32E-05 5.66956E-08
9.22 6.078744E-10 1.65E-05 4.05094E-08
9.31 4.8759978E-10 2.05E-05 2.86159E-08
9.41 3.8966308E-10 2.57E-05 1.98576E-08
9.51 3.0878961E-10 3.24E-05 1.34306E-08
9.62 2.4122E-10 4.15E-05 8.75953E-09
9.73 1.8420436E-10 5.43E-05 5.42271E-09
9.87 1.3568625E-10 7.37E-05 3.10509E-09
10.03 9.4098204E-11 1.06E-04 1.5677E-09
10.23 5.8225517E-11 1.72E-04 6.27175E-10
10.57 2.7113042E-11 3.69E-04 1.415E-10
10.39 1 1.35E-05 -6.58030674E-08 4.11E-11 2.43E-04 3.1982E-10
10.21 6.10E-11 2E-04 6.85989E-10
10.51 3.10E-11 3E-04 1.84048E-10
10.68 2.10E-11 5E-04 8.55671E-11
10.80 1.60E-11 6E-04 5E-11
10.89 1.30E-11 8E-04 3.31388E-11
10.96 1.10E-11 9E-04 2.37897E-11
11.02 9.57E-12 1E-03 1.8046E-11
11.07 8.50E-12 1E-03 1.42524E-11
11.12 7.67E-12 1E-03 1.16077E-11
11.15 7.00E-12 1E-03 9.68533E-12
11.19 6.45E-12 2E-03 8.24074E-12
11.22 6.00E-12 2E-03 7.12527E-12
11.25 5.62E-12 2E-03 6.24427E-12
11.28 5.29E-12 2E-03 5.53505E-12
11.30 5.00E-12 2E-03 4.95474E-12
11.32 4.75E-12 2E-03 4.47315E-12
11.34 4.53E-12 2E-03 4.06854E-12
11.36 4.33E-12 2E-03 3.72489E-12
11.38 4.16E-12 2E-03 3.43019E-12
11.40 4.00E-12 3E-03 3.17529E-12
 1.2

0.8

0.6

0.4

0.2

0
10.00 12.00

αHA- αA-2
81.49556 7.4131E-08
85.0345884 8.5252E-08
88.5438961 9.78977E-08
92.0249719 1.12334E-07
95.4791903 1.28891E-07
98.9078234 1.47992E-07
102.312051 1.70177E-07
105.692969 1.96154E-07
109.0516 2.26866E-07
112.388896 2.63603E-07
115.705747 3.08167E-07
119.002987 3.63162E-07
122.281398 4.32497E-07
125.541713 5.2231E-07
128.784623 6.42837E-07
132.010774 8.125E-07
135.220775 1.06814E-06
138.415194 1.49573E-06
141.594541 2.35318E-06
144.759142 4.93001E-06
147.803923 0.0005068
140.515019 0.0499999
132.795748 0.10218969
124.8109 0.15617426
116.62197 0.21153839
108.29184 0.26785708
99.883304 0.32470583
91.45762 0.38167049
83.0732043 0.43835613
74.7845118 0.49439459
66.6411445 0.54945053
58.6872021 0.60322579
50.9608762 0.65546217
43.4942727 0.70594261
36.3134392 0.75449101
29.4385648 0.80097087
22.8843184 0.84528302
16.6602868 0.88736264
10.771482 0.92717584
5.21888502 0.96471601
7.77547374 0.94743135
11.245726 0.92396956
5.93703897 0.95986069
4.07462401 0.97245216
3.12497216 0.9788726
2.54913801 0.98276571
2.16269717 0.98537837
1.88540763 0.98725308
1.67674708 0.9886638
1.5140428 0.98976381
1.38361847 0.99064559
1.27673468 0.99136821
1.18754566 0.99197121
1.11199324 0.992482
1.04717207 0.99292025
0.9909474 0.99330037
0.94171549 0.99363322
0.89824816 0.9939271
0.85958894 0.99418847
0.82498187 0.99442244
0.79382158 0.99463311
Referencias Bibliograficas
https://www.infisport.com/blog/que-es-la-glutamina-y-para-que-sirve
https://cuidateplus.marca.com/alimentacion/diccionario/glutamina.html
https://es.wikipedia.org/wiki/Glutamina
https://www.medintensiva.org/es-la-glutamina-un-aminoacido-casi-articulo-13110707