Fall 2019 BI208 Food Microbiology Lab Schedule

Bi 208 Food Microbiology Fall 2019

Period 1 Period 2 Lab being
WEEK Assignments
( Wed /Thurs) (Fri) performed
Micro Basics
- What is a
bacteria
Safety Instruction & Class Review safety guidelines
Week1 (Shape and Intro and Safety
Lists and complete online Safety
size) instruction
Discuss Monitoring quiz-due Monday Sept 9th
- Gram
reactions

Lab skills Lab skills crash

Lab skills
Topics covered: course
Topics covered:
-Labelling petri dishes
- Spread Plate vs. Pour Students will have a quiz in
Week2 -Streaking Note: reportable
Plate reportable count Week 5 covering
-Pipette transfers (1mL and count
calculations calculations
0.1mL) calculations will
-Drawing dilution schedules be covered in
lecture/online
Media function and uses
Week3 Microscope and
Lab report writing
Haemocytometer
Theory of Microscope
Start Lab #1: Total Aerobic Complete Lab 1 TAC + B.
Count plates
Week4 Count + B. cereus Lab #1- TAC & cereus Post-lab Online Quiz
Discuss / perform
Incoulcate Petrifilm B. cereus
Calculations

Start Lab #2. Water-Coliform

Lab #2. Water
methods Lab #2 - Read results, Work on Lab #2: Water-
Week5 testing -
E.coli / K.pneumo. spiked water sub BGLB and EMB Coliform Methods work
Coliforms
Incoulcate Petrifilm sheet
MPN & MF

Lab#2 Water – read

BGLB/EMB plates
Calc quiz for
Week6
MF/MPN/Hearmocyto
Introduce Method Comparison
meter (5%)
Assignment (in class) (8.5%)

Lab #3
Start Lab #3 Environmental
Environmental Lab #3
Testing
Week7 Testing Environmental
SWAB TECHNIQUE
AIR SAMPLING Testing
RODAC METHOD

READING WEEK / BREAK WEEK

Week8 Lab #3 Enviro Follow-up Lab #4: Yoghurt Lab 3 Enviro data sheet
Should have fully grown Start Lab #4 post-lab
fungi/sporeformers by now. Yoghurt
 Commercial
Anaerobic Incubation
Count and Calc
Pour plates in Tomato
Juice agar / MRS
Submit Environmental Data
Sheet at end of lab

Lab #4 Yoghurt.
Lab #4 Yoghurt. Count the Anaer plates
Prepare yoghurt from Skim Milk
Week9
Powder Titrate the yoghurt Lab 4 Yoghurt post-lab
Lab #4: Yoghurt
samples quiz + data sheet
Gram Stains

Yoghurt Post Lab Data

Sheet

Yoghurt Post-lab Quiz + data

sheet submission

Start Lab #5: Staph/Strep Lab #5: Staph/Strep

Pudding ... MSA/VJ/BP
spreads, KF Pour plates Sub: Staph to TSA /
Week10 Lab #5: Staph &
BHI
Strep
Sub: Strep to BEA
slant/ BHI+6.5% Salt

Lab #5 Staph/Strep:
Staph:/Catalase /Gram / golden
pigment?
Strep: Esculin-Hydrolysis/
Gram/ Growth in Salt BHI Lab 5 Staph/Strep
Do Thermonuclease and Post-Lab Quiz Open
Coagulase Book
Week11 Lab #6: In-class quiz for Lab
At the end of Lab period… Lab #6 Salmonella 5Staph/Strep
Observe Coagulase, and Salmonella/Listeria
Thermonuclease demo -streak isolated sel/Diff
(prepared) plates

Lab #6: Salmonella/Listeria

-given 1enrich, students prepare
2nd enrich.

Week12 Lab #6: Salmonella/Listeria Lab #6: - Online Quiz ??

 Salmonella/Listeria
Read plates and set up Read results and
confirmation (biochem) identify species

Lab Exam practice

No Lab (instructors
Lab Test (Theory paper based may allow you to
Week13
& Practical demo stations) come see your lab test Lab test
plates)

Week14
Final Test Week- NO LABS
*Note: As part of your lab participation grade, 5 quizzes worth 5% will be randomly given throughout the semester on
any of the lab days. It is important that you come prepared for EVER lab day, as failure to write these quizzes will result
in a -2.5% reduction of your overall grade