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  • Pipette Calibration Check

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    Mark Still Delarna
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    This document provides instructions for calibrating pipettes using a pipette calibration spreadsheet. The spreadsheet requires entering the water temperature, balance used, thermometer used, and weights of five replicates of water dispensed by the pipette. The density is automatically calculated based on the temperature. Acceptance criteria for calibration include maximum percentage difference and percentage relative standard deviation (%RSD) allowed based on pipette type and volume dispensed. Corrective action is required if acceptance criteria are not met.

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    This document provides instructions for calibrating pipettes using a pipette calibration spreadsheet. The spreadsheet requires entering the water temperature, balance used, thermometer used, and weights of five replicates of water dispensed by the pipette. The density is automatically calculated based on the temperature. Acceptance criteria for calibration include maximum percentage difference and percentage relative standard deviation (%RSD) allowed based on pipette type and volume dispensed. Corrective action is required if acceptance criteria are not met.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    18 views1 page

    Pipette Calibration Check

    Uploaded by

    Mark Still Delarna
    This document provides instructions for calibrating pipettes using a pipette calibration spreadsheet. The spreadsheet requires entering the water temperature, balance used, thermometer used, and weights of five replicates of water dispensed by the pipette. The density is automatically calculated based on the temperature. Acceptance criteria for calibration include maximum percentage difference and percentage relative standard deviation (%RSD) allowed based on pipette type and volume dispensed. Corrective action is required if acceptance criteria are not met.

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    © All Rights Reserved
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    of 1

    Effective 

    Date: 06/08/2016 MT‐PIPCAL‐1.3
    Revision Date: 06/07/2016
    Metals Group 
    Revision Author(s): M . Thompson Pipette Calibration Check

    INSTRUCTIONS:
    METALS GROUP PIPETTE CALIBRATION CHECK SPREADSHEET Corrective Action Required for Failing
    Enter the water temperature to the nearest
    Pipets
    Analyst: Balance: 0.5 deg. and the weight of the 5 replicates
    Temp.°C Thermometer: of water dispensed by the pipette.
    See Corrective Action Tab for
    Date: The density is automatically entered
    Resolution
    Water Density (in g/mL) upon entering the temperature.

    Measurement 1 Measurement 2 Measurement 3 Measurement 4 Measurement 5 Adjustable Pipettes; If one of the bracketed
    Pipet Mean Vol. RSD of Vol. % Difference % RSD weights exceeds tolerance, all weight for that
    Pipet ID # Vol. in uL % Difference
    Location Weight (g) Vol. (uL) Weight (g) Vol. (uL) Weight (g) Vol. (uL) Weight (g) Vol. (uL) Weight (g) Vol. (uL) (uL) (uL) PASS/FAIL PASS/FAIL pipette will need corrective action.
    Fixed volume Pipette [ 100uL - 2500uL ]
    % Difference for Fixed volume Pipette
    100uL - 2500uL 0.60%

    % RSD for Fixed volume Pipette


    100uL - 2500uL 5.00%

    Adjustable volume Pipette [ 10uL - 100uL ] % Difference for Adjustable volume Pipette
    10uL - 100uL
    10 3.00%
    50 1.00%
    100 0.80%

    % RSD for Adjustable volume Pipette


    10uL - 100uL
    10 20.00%
    50 10.00%
    100 5.00%

    Adjustable volume Pipette [ 100uL - 1000uL ] % Difference for Adjustable volume Pipette
    100uL - 1000uL
    100 3.00%
    500 1.00%
    1000 0.60%

    % RSD for Adjustable volume Pipette


    100uL - 1000uL
    100 20.00%
    500 10.00%
    1000 5.00%

    Adjustable volume Pipette [ 1000uL - 10000uL ] % Difference for Adjustable volume Pipette
    1000uL - 10000uL
    1000 3.00%
    2000 3.00%
    5000 1.00%
    10000 0.60%

    % RSD for Adjustable volume Pipette


    1000uL - 10000uL
    1000 20.00%
    2000 20.00%
    5000 10.00%
    10000 5.00%

    Adjustable volume Bottle Top Pipetter [1000uL - 100,000uL ] - Checked Quarterly


    % Difference for Bottle Top Pipetter
    1mL -100mL 10.00%

    % RSD for Bottle Top Pipetter


    1mL -100mL 5.00%

    Page 1 of 3 MT‐PIPCAL‐1.3.xlsx

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