Marks Scored:

KATHMANDU UNIVERSITY
End Semester Examination
August/September, 2016
Level : B. Tech. Course : BIOT 308
Year : III Semester : II
Exam Roll No. : Time: 30 mins. F. M. : 20

Registration No.: Date :

SECTION "A"
[10 Q × 1 = 10 marks]
Give the most appropriate answer:

1. Epimerases belongs under the subclass of _____________ enzyme.

a. Isomerases b. Hydrolases c. Transferases d. Ligases

2. Which of the followings is not a method of separation of an enzyme based on their

polarity?
a. Ion-exchange chromatography b. Isoelectric focusing
c. Affinity chromatography d. Electrophoresis

3. A cofactor required for Nitrogenase reaction is _____________

a. Mg2+ b. Biotin c. Fe d. Lipoic Acid

4. Which of the following amino acids is acidic in nature?

a. Arginine b. Lysine c. Glutamate d. Glutamine

5. Which of the followings is not the commonly used chemical denaturants?

a. Urea b. GnCl c. DMSO d. ZnCl2

6. Aspartate carbamomyltransferase is inhibited by ___________during the biosynthesis

of pyrimidine in E.coli.
a. UTP b. CTP c. UMP d. TDP

7. SDS binds tightly to proteins, normally in a ratio of about one SDS molecule for
every _________amino acids in a protein.
a. 4 b. 3 c. 2 a. 1

8. When inhibitors bind only to the enzyme-substrate complex and not to the free
enzyme, then the type of inhibition is known as
a. Competitive b. Non-competitive c. Uncompetive d. irreversible

9. Which of the following terms is not correlated with a substrate with single
asymmetirc centre?
a. Enantiomer b. Diastereomer c. Optically active d. Racemases

10. During the conversion of 4-keto-6-deoxyglucose and NADPH to NADP+ and dTDP-
L- rhamnose, the inversion of configuration takes place in --------------------positions.
a. C-4 b. C-3 c. C-5 d. C-3 and C-5
 SECTION “B”
[10Q  1 = 10 marks]

11. The phosphorylase “b” is activated in presence of _________________

12. When CH3CH2X reacts with KOH/Alcohol, the reaction product is ______________

13. Cytochrome P450 have monooxygenase activity which gets electrons initially from
___________________ to molecular oxygen.

14. Phosphorylase “a” converts into Phosphorylase “b” in presence ___________enzyme.

15. _____________antagonizes the effects of pyrimidine nucleotides and restore the

activity of carbamoyl phosphate synthase in E.coli.

16. If argine is present in excess, _____________that catalyzes the production of N-

acetly-L-glutamate in E.coli will be feedback inhibited.

17. ____________is an alternative to the aminotransferase for assessing liver damage.

18. In normal serum the ration of LDH-1: LDH-2 activity is equal to ______________.

19. Conversion of D-glucose-1-phosphate to UDP-glucose takes place in presence of

enzyme _________________.

20. Enzymes that contain tightly bound metal ions through the co-ordinate bond, most
commonly transition metals are known as ________________.
 KATHMANDU UNIVERSITY
End Semester Examination
August/September, 2016
Level : B. Tech. Course : BIOT 308
Year : III Semester : II
Time : 2 hrs. 30 mins. F. M. : 55
SECTION “C”
Attempt ANY SIX questions:

1. What are epimerases? Draw the proton transfer mechanism of epimerization. [2+3=5]

2. What is extrinsic control of an enzyme? What are the four major physiological
advantages of this kind of control mechanism? [1+4=5]

3. What are the major ways of immobilization of enzyme during the production of
syrups from corn starch? [5]

4. A plot of velocity against substrate concentration for enzymatic reaction containing

effectors is sigmoidal rather than hyperbolic. Explain. [5]

5. How is phosphorylase switched off? State the properties of glycogen synthase. [2+3=5]

6. How does the activity of hexokinase is regulated in glycolytic pathway? [5]

7. What are biosensors? How it works? State the role of Metaloenzymes and Metal-
activated enzymes as chemical denaturant. [5]

8. Outline the scheme for indirect determination of amino acid sequence of a protein. Point
out the complementary roles of direct and indirect sequencing of proteins? [2+3=5]

SECTION “D”

Attempt ANY FOUR. Q. No. 13 is compulsory.

9. What are the major functions of an enzyme? Discuss the structural features of
secondary structure of a Protein. [2+4=6]

10. How do the enzyme -glutamyltransferase is diagnosed in liver disease? What are the
conditions that have to be fulfilled as routine diagnostic clinical methods to measure
the enzyme activity? [2+4=6]

11. In metabolic pathway control mechanism, if an intermediate B produced from A is

required for the synthesis of two products, say D & E, then it would seem reasonable
that there would be some control mechanism to allow the rate of production of C to be
altered to deal with various situations. Explain the statement with specific example.
[6]
12. State the Michaelis-Menten equation and correlate it with the need of Lineweaver
Burk plot. [6]

13. Write short notes on- [3.5  2=7]

a. Enzymatic cancer therapy
b. Mechanism of Covalent catalysis