BAB 3: EKSPERIMEN MENGKAJI ORGANISMA UNISEL

You are required to prepare and observe unicellular organism under the light microscope.
Anda dikehendaki untuk menyediakan dan memerhatikan slaid organisma unisel di bawah mikroskop
cahaya.

Prepare the report that contain all the given aspect:

Sediakan laporan yang mengandungi aspek-aspek yang berikut:

Title
Tajuk

Objective
Tujuan

Apparatus and materials

Bahan dan radas

Procedure
Prosedur

Data representation
Persembahan data

Discussion
Perbincangan

1. Compare and contrast the structure of Amoeba sp.and Paramecium sp.

Banding dan bezakan struktur Amoeba sp. Dan Paramecium sp.

2. How the movement of Amoeba sp. is different from Paramecium sp.?

Bagaimanakah pergerakan Amoeba sp. berbeza daripada Paramecium sp.

3. Both organism had contractile vacuole. What is the function of contractile vacuole?
Kedua-dua organisma mempunyai vakuol mengecut. Apakah fungsi vakuol mengecut?

Conclusion
Kesimpulan
SUGGESTED ANSWER (CADANGAN JAWAPAN)

CATEGORY B (KATEGORI B)

TITLE Investigate the unicellular organism

TAJUK Mengkaji proses hidup organisma unisel
OBJECTIVE To prepare and observe the unicellular organism under light
microscope
TUJUAN Menyedia dan memerhatikan organisma unisel di bawah mikroskop 1m
cahaya.

MATERIALS Amoeba sp. culture, Paramecium sp. culture, cotton, sodium chloride
solution and vinegar. 1m
BAHAN Kultur Amoeba sp., kultur Paramecium sp., kapas, larutan natrium
klorida dan cuka

APPARATUS Glass slide,cover slip, light microscope, dropper, needle

RADAS Slaid kaca, kaca penutup, mikroskop cahaya, penitis dan jarum tenggek

PROCEDURE
PROSEDUR dropper

1m

Culture of Amoeba
sp.

Properly, place the cover

slip

Observe under light microscope

1. Amoeba sp culture and Paramecium sp culture is prepared by

teacher a week before the experiment.
1. Kultur Amoeba sp., kultur Paramecium sp. disediakan oleh guru
 seminggu sebelum eksperimen.

2. A piece of cotton wool is placed onto the glass slide. Cotton wool will
limit the movement of Amoeba sp.
2.Sedikit kapas diletakkan pada slaid. Kapas akan mengehadkan
pergerakkan Amoeba sp.

3. A drop of Amoeba sp is placed onto the cotton wool and covered by 1m
glass slide
Precaution step; Makesure there are no air bubbles in the slide
3.Setitis kultur Amoeba sp. diletakkan pada kapas dan ditutup dengan
penutup kaca.
Langkah berjaga-jaga: Pastikan tiada gelembung udara yang terhasil 1m
semasa menyediakan slaid.

4. Amoeba sp structure is observed under the light microscope

4.Struktur Amoeba sp. diperhatikan menerusi mikroskop cahaya. 1m

5. Amoeba sp. structure is drawn and labelled

5.Struktur Amoeba sp. dilukis dan dilabelkan

6. The movement of Amoeba sp is observed

6.Bagaimana Amoeba sp. bergerak diperhatikan

7. A drop of sodium chloride is placed at the side of cover slip.

7.Setitis larutan natrium klorida diletakkan pada sisi kaca penutup.

8.The reaction of Amoeba sp toward the sodium chloride is observed.

Step 7 is repeated by replacing sodium chloride with vinegar.
8.Cara Amoeba sp. bergerak balas terhadap larutan natrium klorida
diperhatikan. Langkah 7 diulang dengan menggantikan larutan natrium
klorida dengan cuka.

9.Step 2 and 7 is repeated by replacing the Amoeba sp culture with

Paramecium sp culture
9.Langkah 2 hingga 7 diulang dengan menggantikan kultur amoeba sp
dengan kultur Paramecium sp.

DATA
PRESENTATION 1m
PERSEMBAHAN 1m
DATA (lukis dan
label)

Amoeba sp.
 Paramecium sp.

DISCUSSION 1. Banding dan bezakan struktur Amoeba sp. Dan Paramecium sp.
PERBINCANGAN
ASPECT Amoeba sp Paramecium sp.
ASPEK 1m
SIMILARITIES/PERSAMAAN
Both have contractiile vacuole
Mempunyai vakuol mengecut
Waste product is secreted by diffusion through plasma
membrane
Bahan buangan dibuang melalui plasma membran
melalui proses resapan
Gas exchange through diffusion
Pertukaran gas respirasi secara resapan

DIFFERENCE/PERBEZAAN
SHAPE/ No fix shape Slipper shape
BENTUK Tiada bentuk tetap Berbentuk selipar
NUCLEUS/ Exist Macronucleus and
NUKLEUS ada micronucleus
Mempunyai 2 nukleus;
makronukleus dan
mikronukleus
REPRODUCT Aseks Seks
ION/
PEMBIAKAN
NUTRITIO/ Eat by phagocytosi Pukulan silia yang beritma
PEMAKAN process. Pseudopodium menolak makan masuk ke
AN is projected towards dalam sitostom
food.
Makan melalui proses
fagositosis.
Pseudopodium
dihulurkan untuk
mengelilingi makanan

2. How the movement of Amoeba sp is different from

1m
Paramecium sp?
Bagaimanakah pergerakan Amoeba sp. berbeza daripada Paramecium
 sp?
Amoeba sp moving by projected it pseudopodium towards the place it
want to go, cause the sitoplasm flow following the pseudopodium.
Others pseudopodium is pulled towards it. Othertwise Paramecium sp
moving by the beat of the cilia make it splin along it axis when moving
forward.
Amoeba sp. bergerak dengan menghulurkan psedopodium ke arah
pergerakkan menyebabkan sitoplasma mengalir ke dalam
pseudopodium yang dihulurkan. Pseudopodium lain ditarik semula.
Manakala Paramecium sp. Bergerak melalui pukulan beritma silia yang
menghasilkan gelombang dan meyebabkan Paramecium sp. Berputar
dan berpilin sepanjang paksinya semasa bergerak ke hadapan.
1m
3. Both organism have contractile vacuole. What is the function of
contractile vacuole?
Kedua-dua organisma mempunyai vakuol mengecut. Apakah fungsi
vakuol mengecut?

Contractile vacuole functioning for osmoregulation. Water that diffuse

into these organism will accumulate in contractile vacuole. When
reaching the maximum size, vacuole will contract to excrete it content.
Vakuol mengecut berfungsi untuk pengosmokawalaturan. Air yang
masuk ke dalam organisma unisel ini akan dikumpul di dala vakuol
mengecut. Apabila mencapai saiz maksimum, vakuol ini akan mengecut
untuk mengeluarkan kandungan di dalamnya.

CONCLUSION Amoeba sp ad Paramecium sp have contractile vacuole for 1m

KESIMPULAN osmoregulation process. Amoeba sp reproduce by asex, no fix shape,
have a nucleus and eat by phagocytosis. While Paramecium sp
reproduce by sex reproduction, shape like slipper, have 2 nucleus and
eat by using the beat of cilia which push it food toward the cytostom.
Amoeba sp dan paramecium sp mempunyai vakuol mengecut untuk
proses pengawalaturan. Amoeba sp membiak secara aseks, tiada
bentuk tetap, mempunyai nukleus dan makan melalui proses
fagositosis. Manakala paramecium sp menjalankan pembiakan seks,
berbentuk selipar, mempunyai 2 nukleus serta makan menggunakan
pukulan silia yang menolak makanan ke dalam sitostom

Markah PKS 12
Kemahiran manipulatif 2
Nilai murni 1
Markah diperolehi 15