Form 4 Biology

1.2 itStudying
ICT ivitkA the living processes of
Observation
2.1 unicellular organisms

Materials
Amoeba sp. culture, Paramecium sp. culture, cotton wool, sodium chloride solution and vinegar
Apparatus
Glass slides, cover slips and a light microscope
Procedure
1 Your teacher will prepare the Amoeba sp. culture and Paramecium sp. culture a week before
the experiment.
2 Spread some cotton wool on the slide. The cotton wool will limit the movement of Amoeba sp.
3 Place a drop of the Amoeba sp. culture onto the cotton wool and cover it with a cover slip.
4 Observe the structure of Amoeba sp under the light miscroscope.
5 Draw and label the structure of Amoeba sp. Take note!

6 Observe how Amoeba sp. moves. You can prepare

7 Place a drop of natrium chloride solution on the side of the cover slip. the Amoeba
Observe how Amoeba sp. reacts to the solution. sp. Culture and
Paramecium sp.
8 Repeat step 7 by replacing the natrium chloride solution with vinegar.
Culture by boiling
9 Repeat steps 2 to 7 by replacing the Amoeba sp. culture with the some dry grass in
Paramecium sp. culture. water. Leave it in a
basin for one to two
Discussion weeks until a layer
1 Compare and contrast the structures of Amoeba sp. and of bacteria grows
Paramecium sp. under the surface
2 How does the movement of Amoeba sp. differ from that of of the water. Then,
Paramecium sp. add some pond
3 What are the reactions of both organisms when confronted with an water containing
obstacle (cotton wool) and chemicals (salt solution and vinegar)? What Amoeba sp. and
Paramecium sp.
inferences can you make based on these observations?
to it.
Conclusion
Give a suitable conclusion for this activity.