Professional Documents
Culture Documents
for media preparation, tubes, dropper pipettes and centrifuge tubes, test
tubes for culture maintenance.
1.0 OBJECTIVE:
To lay down a Procedure for provide guidelines for Cleaning of
Glassware for Microbiological testing.
2.0 SCOPE:
This procedure is applicable for cleaning of all Glassware, which is
used for Microbiological analysis in Microbiology Laboratory of Quality
Control Department
3.0 RESPONSIBILITY:
Microbiologist
4.0 ACCOUNTABILITY:
Head -QA & QC
5.0 PROCEDURE:
5.1 Tubes:
5.1.1 Empty the contents and wash with tap water.
5.1.2 Immerse the tubes in tub containing 1%v/v (1 ml in 100 ml) soap
solution for 30 minutes.
5.1.3 Remove the tubes, wash with tap water.
5.1.4 Finally rinse with freshly collected distilled water.
5.1.5 Dry the tubes in drying oven at 160°C for 2 hrs.
5.3 Pipettes:
5.3.1 Wash with tap water and immerse them in 1%v/v soap solution for
30 minutes.
5.3.2 Wash each pipette first with tap water and then with hot water till
all traces of soap are removed.
5.3.3 Finally rinse with freshly collected distilled water.
5.3.4 Dry the pipettes as per SOP.
5.3.5 Store the pipettes in stainless steel container in microbiology
section.