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A stain e.g. aceto-orcein is added and the root tip macerated using a scapel.
Pressing the cover slip gives a thin squash, which is observed under the microscope.
A piece of moist bread is placed on a petri-dish or enclosed in a plastic bag and observe
daily for four days.
Under a low power microscope the sporangia and stolons can be observed.
Detach a frond from the plant and observe the under-side using a hand lens to see the
raised brown patches - the sori.