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D-218
3.14 Calibration Specific
Analysis parameters such as the calibration type and approximation, etc., can be set in advance.
These settings can also be outputted in a data list.

Main operation flow Described in

(P) Parameters another section.
One of the tabs
Operation flow
can be selected
(B)Calibration
Press this key. Button function
(I)Calibration Specific

"Calibration Specific" screen

Select [General] tab Select [ISE] tab

Referring to a Referring to an
biochemical test ISE (optional unit)
test

F4 F2 F4 F2

Setting a Setting an
biochemical test ISE (optional unit)
test

F8 F8 F3 F3

Specifying another Outputting the

analysis test setting contents in
a data list

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■ “Calibration Specific : General tab” screen
If the [Calibration Specific] menu is selected, the analysis parameters in a biochemical test for
calibration analysis are displayed in the “Calibration Specific : General tab” screen. At this time the
tab of “General” is selected.

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Calibration Specific
General ISE
Test Name: GOT Type: Serum Use Serum Cal.

Calibration Type: Formula: Counts: Process: CONC

Cal. No. OD CONC Factor/OD-L Factor/OD-H

Point 1:
Point 2:
Point 3:
Point 4:
Point 5:
Point 6:
Point 7:
1-Point Cal. Point: with CONC-0
MB Type Factor: Calibration Stability Period:

Help Exit Print Set Test No.

ALARMCLEAR

F4 (Set)

Help Exit Test No.

ALARMCLEAR

● Test Name
Displays the selected test name. Select a test name from the pull-down list.

● “<<” button / “>>” button

Each time either of these buttons is selected, the test name being displayed is switched.
It is also possible to switch test name by keyboard operation.
Shift key + Page Down key :Same as the function of the “<<” button
Shift key + Page Up key :Same as the function of the “>>” button

● Type
The sample type can be switched. Select either “Serum”, “Urine”, or “Other” from the pull-down list.

● Use Serum Cal.

If this check box is checked with a mark (selected), the data is calculated in accordance with the serum
calibration during analysis of urine samples.

● Calibration Type
Select the calibration type for each analysis test from the pull-down list.

See Also
About calibration types, see “■ Summary of Calibration Types”.

● Formula
Select an equation of approximation according to the calibration type from the pull-down list.

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● Counts
Set the number of times to repeatedly perform calibration analysis and a reagent blank analysis.
Depending on the number, the following values will be used:
Set to 2: An average of two values
Set to 3: An average of two close values
Set to 4: An average of two values, excluding maximum and minimum values
Enter a numerical value between 1 and 4.
● Process
Set the method to be used if an update of calibration by calibration analysis fails. Set to either “OD”
or “CONC”.
To perform a concentration conversion using the existing factors, set to “CONC”. Otherwise, set to
“OD” if a concentration conversion is not initially performed. Save the measured OD value, and batch-
perform the concentration conversion after re-performing calibration analysis.
This can be set only if the calibration type is set to ACAL (AB, AA, 2AB ~ 7AB).
● Cal.No. at each Calibrator specification point
The entered numbers are assigned to either of the sample types, as follows.
1 to 50 : Serum
51 to 70 : Urine
71 to 200: Other

Tip
A calibrator No. is displayed with a 2-digit cord. For a number between 1 and 99, it is displayed with
a numeric value as it is. For a number between 100 and 200, it is displayed with a specific cord that
includes an alphabet, from between A0 and K0.

See Also
For information about the relationship between a calibration No.and an input value, refer to“Part C 2.6
Preparing the Sample and Rack ■Relationship between a Calibrator No. and an Input Value”.

● Cal.No. of Calibrator specification point 1

This can be set only if the calibration type is set to 2MB ~ 7MB, AB, AA or 2AB ~ 7MB.
● Cal.No. of Calibrator specification point 2
This can be set only if the calibration type is set to 2MB ~ 7MB, AA or 2AB ~ 7AB.
● Cal.No. of Calibrator specification point 3
This can be set only if the calibration type is set to 3MB ~ 7MB or 3AB ~ 7AB.
● Cal.No. of Calibrator specification point 4
This can be set only if the calibration type is set to 4MB ~ 7MB or 4AB ~ 7AB.
● Cal.No. of Calibrator specification point 5
This can be set only if the calibration type is set to 5MB ~ 7MB or 5AB ~ 7AB.
● Cal.No. of Calibrator specification point 6
This can be set only if the calibration type is set to 6MB ~ 7MB or 6AB ~ 7AB.
● Cal.No. of Calibrator specification point 7
This can be set only if the calibration type is set to 7MB or 7AB.
● OD value at each point
Set the calibrator OD value within the range between -2.0 and 2.5 at each point.
● CONC at each point
Set the calibrator concentration value within 7 effective digits at each point.

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● Lower limit of a factor range or absorbance range of each Calibrator specification point
This can be set only if the calibration type is set to ACAL (AB, AA, 2AB ~ 7AB).
Set a factor range if the approximation is a line, and set a absorbance range if the approximation is a
curve.
● Upper limit of a factor range or absorbance range of each Calibrator specification point
This can be set only if the calibration type is set to ACAL (AB, AA, 2AB ~ 7AB).
Set a factor range if the approximation is a line, and set a absorbance range if the approximation is a
curve.
● 1-Point Cal. Point
This can be set only if the calibration type is set to 2AB ~ 7AB.
Set a value less than the maximum point that is specified according to the calibration type.
● with CONC-0
If there is a standard solution with a concentration value of 0, calibration can be performed at
concentration value 0 and at 2 other points. If calibration is made at 2 points, check the check box.
Each time the Space key is pressed, the check box status is switched between “Checked” and
“Unchecked”.
● MB Type Factor
This can be set only if the calibration type is set to MB.
● Calibration Stability Period
Set the calibration stability period of the created calibration chart within the range between 1 and 999
days. To set this period to “None”, enter a space.

■ F3 : Print - in the “Calibration Specific : General tab” screen

Setting contents can be outputted in a data list.
1. Place paper into the printer.
2. Press the F3 (Print) key.
A window to start output is displayed.
3. Select the “OK” button.
Output starts.

To interrupt data output, select the “Cancel” button during output.

■ F4 : Set - in the “Calibration Specific : General tab” screen

To set individual test parameters for a biochemical test, observe the following procedure.
1. Specify an analysis test for which analysis parameters are to be set.
For the specifying procedure, see “■ F8 : Test No.”.
2. Set the sample type.
Select either “Serum”, “Urine”, or “Other” from the pull-down list in the “Sample type” field.
3. Press the F4 (Set) key.
4. According to the messages displayed, set the analysis parameters.
5. After the setting has been completed, press the F2 (Exit) key.
The parameters set in this process will be registered.

■ F8 : Test No.
It is possible to switch to other analysis test screen than the currently displayed one.
1. Press the F8 (Test No.) key.
The “Select Test No.” dialog box is displayed.
2. Enter a test No. and select the “OK” button.
The selected analysis test is displayed.

Tip
The analysis test can be also specified from the pull-down list in the “Test Name” field, or with the
“<<” or “>>” button.

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■ “Calibration Specific : ISE tab” screen
The individual test parameters of each analysis test categorized as an ISE (optional unit) test can be
referred to. Change the display to the “Calibration Specific : ISE tab” screen by selecting the “ISE”
tab.

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Calibration Specific
General ISE
Test Name.: Type: Serum

Calibration Type: ACAL Offset

Counts:

Factor A: 10.00000
B: 0.000000

Calibration Selection:
Cal. No. CONC Factor-L Factor-H

Help Exit Print Set

ALARMCLEAR

F4 (Set)

Help Exit

ALARMCLEAR

● Test Name
Displays the selected test name. Select a test name from the pull-down list.

● “<<” button / “>>” button

Each time either of these buttons is selected, the test name being displayed is switched.
It is also possible to switch test name by keyboard operation.
Shift key + Page Down key :Same as the function of the “<<” button
Shift key + Page Up key :Same as the function of the “>>” button

● Type
The sample type can be switched. Select either “Serum”, “Urine”, or “Other” from the pull-down list.

● Calibration Type
Select the calibration type for each analysis test from the pull-down list.

See Also
About the calibration types, see “■ Calibration Processing on the ISE”.

● Counts
Enter the frequency of multiple measurements within the range of 1 to 4.

● Factor A, B
Set calibration factor A and calibration factor B within 7 effective digits.

● Cal No.
Set a calibrator No.

● CONC
Set a calibrator concentration value within 7 effective digits.

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● Factor-L
Set the lower limit of a factor range within 7 effective digits.

● Factor-H
Set the upper limit of a factor range within the range between the lower limit and 7 effective digits.

■ F3 : Print - in the “Calibration Specific : ISE tab” screen

Setting contents can be outputted in a data list.
1. Place paper into the printer.
2. Press the F3 (Print) key.
A window to start output is displayed.
3. Select the “OK” button.
Output starts.

To interrupt data output, select the “Cancel” button during output.

■ F4 : Set - in the “Calibration Specific : ISE tab” screen

To set the individual test parameters of each analysis test in the ISE test, observe the following
procedure.
1. Specify an ISE test to be set from the pull-down list in test name field or with the “<<” or “>>”
button.
2. Press the F4 (Set) key.
3. According to a message displayed, set the analysis parameters.
4. After the setting has been completed, press the F2 (Exit) key.
The parameters set in this process will be registered.

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■ Summary of Calibration Types
This system allows the operator to generate a maximum of 15 types of calibrations, depending on the
analysis test. Of them, 6 major types of calibration will be described in the following.

● ACAL AA

OD • Enter two kinds of standard liquid with a

different concentration or a value of the
ACAL OD2 calibrator, then set a calibration using
an OD value obtained from the automatic
calibration setting function.

ACAL OD1 • This can be applied to analysis items where

the calibration chart is a line but does not
pass through the origin (reagent blank).
(An example analysis item: Calcium)

CONC
Concentration Concentration
value 1 value 2

● ACAL AB

OD • Enter the value of one kind of standard liquid

or the calibrator, then set a calibration
chart using an OD value obtained from the
ACAL OD automatic calibration setting function.

• This can be applied to general calibrations

which are lines and pass through the origin
(reagent blank).

CONC
Concentration value

● ACAL 7AB

OD

OD 7 • By using 2 to 7 kinds of standard solutions

OD 6 or calibrator, and by entering the measured
OD values and concentration values, set a
OD 5 calibration chart.
OD 4
• This will be used for immunity items
and turbidmetry items.
OD 3
OD 2

OD 1
OD 0
Concentration value 1

Concentration value 2

Concentration value 3

Concentration value 4

Concentration value 5

Concentration value 6

Concentration value 7

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● MCAL MB

OD • Set the calibration coefficient with the K factor.

• This is typically used for enzyme items

through the rate assay method.

CONC

● MCAL 7MB

OD • With a maximum of 7 points OD values and

the entered concentration value, set a calibration.

OD 7 • This will be applied to analysis items whose

OD 6 calibration is not a line but each of the curved
sections has a constant slope.
OD 5
Typically used for turbidmetry and immunity items.
OD 4
OD 3
OD 2
OD 1
CONC
Concentration value 1
Concentration value 2

Concentration value 3

Concentration value 4
Concentration value 5
Concentration value 6
Concentration value 7

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● ACAL nAB (single-point correction processing)
First, analyze one of multiple standard solutions for 2AB to 7AB. By using the ratio between the
reaction OD values of this standard solution and the previously measured standard solution, correct the
reaction OD values of other standard solutions, and then recreate the calibration chart.
Also, it is possible to correct the calibration chart with two points of OD0 and another OD value, if the
standard solution with a concentration of 0 is available.

Example 1: If none of multiple standard solutions has a concentration of 0

OD • If the single-point correction is performed

for the CONC2 standard solution, execute
OD3' the following calculation.
OD3
1. Perform the single-point correction.
OD2'
OD2 ODn' = ODn x OD2
OD1' OD2'
OD1 2. Recalculate for the calibration chart.

CONC
0 CONC1 CONC2 CONC3

Example 2: If any standard solution has a concentration of 0

OD • If correction is performed with two points

of CONC1 and another (CONC3), execute
OD4' the following calculation.
OD4
1. Use the reaction OD values of CONC1
OD3' and CONC3 (OD1' and OD3') as they are.
OD3
2. Correct each point as follows.
OD2'
OD2
OD1' ODn' = a x (ODn - OD1) + b
OD1
OD3' - OD1'
CONC a =
0 CONC2 CONC3 CONC4 OD3 - OD1
b = OD1'
ODn : previous OD value ODn' : OD value after correction

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■ Calibration Processing on the ISE
During calibration of electrolyte measurement equipment (ISE), both MID liquid and external standard
liquid (HIGH, LOW), which have a known concentration, are measured. The relationship between the
electrode potential and ion concentration at that time is obtained, and Na, K, and Cl calibration setup
coefficient S (slope) is calculated.

● Manual calibration

Potential
difference (mV) Calibration CH : A known concentration of HIGH STANDARD
liquid used for calibration
EH-EM'
CL : A known concentration of LOW STANDARD
liquid used for calibration

EL-EM'' EH-EM' : A potential difference between HIGH

STANDARD liquid and MID liquid

EL-EM'': A potential difference between LOW

STANDARD liquid and MID liquid
Concentration
CL CH value Set a calibration using a potential difference
(logarithm) between the two points of known concentration.
mmol/L
The slope check and update measurement in calibration are performed as described below.
• Slope check
Measure each standard solution once, and then check if the two kinds of measured values of H-STD
and L-STD are both within the limit values based on the previous calibration values. If both are
within the limit values, the measured values are judged as unfluctuating in comparison with the
previous ones.
If measured values are judged as unfluctuating, four-time measurements for each standard solution
necessary for calibration update after slope check can be omitted. If this is the case, the previous
slope value and MID solution correction factor are output as the calibration results.
• Calibration update measurement
If measured values are judged as fluctuating in comparison with the previous ones as the result of
the above slope check, each standard solution is measured four times, and the calibration is updated.

● Correction by M-CAL
M-CAL at the ISE is data correction using a calculation formula, Y = AX + B.
Coefficients A and B are obtained in the following way.
The following formula is based on the correlated regression between a measurement value from this
System without an M-CAL data correction and that using the conventional method or basic method.
Y = aX + b
Y is a measurement from this System, and
X is a reference value using the conventional method or basic method.
Transform the above equation,
X = (1 / a) Y - (b / a).
Thus, A = 1 / a, B = - b / a.

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● Correction by A-CAL

Y (measured value) • Corrected value: c' - c

Y=aX+b
c' • b'=b-(c'-c)
Y=aX+b'
c a = factor
b = offset

b
b'
X (known concentration of a specimen subject to A-CAL)
C
Let a known concentration of a specimen for A-CAL be C, and let the measurement obtained by this
system from a specimen for A-CAL after an M-CAL correction be C´.
In order to make the known concentration of the specimen for A-CAL and a measurement obtained by
this System consistent, correct the difference between C and C´ using A-CAL. In this case Y = aX + b
will be
Y = aX + b´.
This A-CAL correction can be applied to values that have been subject to an M-CAL correction.

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