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BA400 EUG v 2.
0 en
This document has been designed to be a guideline from version 3.1 of BA400 User Software
BA400 EUG v 2.0 en
First Steps
1. Check levels of Washing Solution ( 25 mL AC16434 / 5L distilled water ) and High Concentration Waste . Bear in mind to confirm
your actions by pressing ‘the bottle’ button when you have filled up the container. This button resets the scales.
2. You must go to Tools → Change reaction rotor (only in ‘standby’ mode) in order to swap it by another new one. Press ‘the up
arrow’ button to release the washing station from the reaction rotor cover. Press ‘the gear wheel’ button in order to come back
at the initial conditions. The program will execute a centering and adjusting of optical reading.
3. Check all the covers are correctly closed (Reaction and reagent rotor covers are critical !)
4. Connection with the PC is possible when the beep sounds. Bear in mind to press ‘the circled double arrow’ button to restore
communications or when you have entered the user software before to switch on the analyser.
5. Run a conditioning (the ‘blue drop’ button) from the menu Tools daily and every time you have filled up the Washing Solution
container.
6. Product reference (5412/5408) is necessary to perform manual pre dilutions of urine samples in a 1:10 ratio for ISE tests.
7. Automatic ISE washing cycle is required every time you have run at least 50 ISE preparations. It’s recommended you request an
Automatic washing cycle by yourself (Tools → ISE Utilities → General → Wash) if software does not request it by itself.
BA400 EUG v 2.0 en
Usual Routine Workflow
BA400 EUG v 2.0 en
Calibrators Programming
Name
Lot
Programming Calibrators Calibrator by test - sample
Expiry
Nº
Any modification of calibrator Select the tests by

leads to new concentration Select the calibrator from the list
values for every associated double-clicking or
tests.
Calibration curve: Increasing

1 point cal multipoint cal
or Decreasing
In multipoint calibrators,
calibrator nº5, or that which Type of calibration curve (Polygonal, Spline,
appears the first one at the list,
Linear regression, Parabolic regression)
is the one with the highest
concentration (factor 1).
Concentration Value/s
BA400 EUG v 2.0 en
Controls Programming
Name
Level*
Programming Controls Lot number Tests
Sample type
Expiry
Tests
Insert minimum and maximum margins for every test
Selection
Level*: Numbering The option ‘Activate Quality

from 1 to 3. It is Control’ from the tab Quality
related with the Control at Programming →
possibility of control Tests must be selected for all
request by level those tests to run the QC.
BA400 EUG v 2.0 en
MANUAL working mode
Reagent blank request
Select blank from the

drop-down list
*: The positioning of distilled water or saline is only
required to run blanks.
Tests
Select all these tests to
run a blank *
Pre selection checking of blanks

in Blanks, Calibrators, controls tab
+
or
You are able to reorder the tests and their availability by
clicking from Programming→ Tests
BA400 EUG v 2.0 en
MANUAL working mode
Calibrations request
Select calibration from Select Sample type from the

the drop-down list drop-down list
Tests
Select all the tests to
run
Pre selection checking of calibrators

in Blanks, Calibrators, controls tab
+
or
BA400 EUG v 2.0 en
MANUAL working mode
Controls request
Select control from the Select Sample type from the

drop-down list drop-down list
Tests
Select all the tests to
run
Pre selection checking of controls in

Blanks, Calibrators, controls tab
+
or
BA400 EUG v 2.0 en
Storing a work session list
Suggest a name for the

Schedule a list of sample tests in
current session at the
MANUAL mode ( see slides 5-7 and 9 )
pop up window
Note this feature is designed to

avoid the same daily manual
requesting (e.g.: Control
sessions)
Go to WorkSession to restore it Load Session Select the session from

the drop-down list
ONLY if there is not an active session
BA400 EUG v 2.0 en
MANUAL working mode
Sample request
Select Patient from Enter sample ID in Patient/Sample field or

Sample Class Tick Urgent if proceeds select it from Patients database ...
Tests Select Sample type
Select all the test to run
or
Check all your requests are selected in

You are able to change patient status by double-
Patient Samples +
clicking at the column Urgent corresponding to
the Sample ID you want to change (Routine in
blue colour and Urgent in pink colour).
You can change the type of sample container from
or
tube to pediatric well or reverse by selecting it at
the drop-down list at the column Tube.
BA400 EUG v 2.0 en
AUTOMATIC Working mode (connected to LIS)
Automatic Start: This feature allows, only if we have

selected Automatic checking barcodes at Worksession This feature allows to download all the pending requests
analyser configuration, to ask pending orders from the even we don’t have available samples.
LIS by sample barcode reading. The session autostarts if all You are able to edit the orders before to position the
there is not pending position requests. samples.
The pause function is only available when the instrument

is in ‘Running’ mode. Its functionality is directly linked to
the addition and subtraction of samples/reagents in We can launch a query by work session. Sample rotor will
continuous mode during the current session. Only be readed and new orders will be added to the current
pending status samples are allowed to change their session. This is almost the same as to launch a pause
positions (green coloured in positioning rotor and in with the addition of not to lose time requires to read all
work session list). The pausing doesn’t suppose to loose the entire sample rotor..
reading cycles. Process is shut off before starting any
reaction (by addition of sample or R2)
This button is only active when there are pending orders
from the LIS. By pressing the button, the new orders are
added to the current session.
Continue button appears after triggering of the pause.
After pressing it, reaction and sample barcodes are
checked out again.
BA400 EUG v 2.0 en
Working with results
AUTOMATIC repetition mode (Configuration → General →

Automatic Processing of Repetitions + Programming → Tests →
Select Test → Procedure → Automatic Repetition)
The instrument repeat automatically the analysis when the result is over limit of
linearity, below limit of detection or, in case of kinetics, when there is an alarm for
Substrate Depletion.
Check the reason of

the warning
MANUAL mode
for repetitions
BA400 EUG v 2.0 en
ISE Utilities
Tools ISE Utilities Installation/ Activation: Execute all the steps in the order of appearance (only
when the module is installed for the first time)
Maintenance: Special function that leaves ISE module out of

1: The instrument warns you General: order for maintenance tasks.
about the need to perform an REMEMBER FIRST TO TURN THE ISE SWITCH OFF
automatic washing after 50 ISE
preparations.
Wash: Automatic washing of ISE channel 1
2: The instrument auto
calibrate electrodes every 4 h only if
Calibrate bubbles4: It checks the absence of air bubbles.
there are ISE tests pending.
3: The instrument auto Calibraciones: Calibrate electrodes2

calibrate pumps every 24 h when
there is a worksession pending even
there are no ISE tests included. Calibrate pumps3
4: Calibration bubbles is SEE THE
included in weekly maintenance (not FOLLOWING
automatic) PAGE
BA400 EUG v 2.0 en
ISE Utilities (cont.)
Replace reagent pack: Execute all the steps in order of appearance in order
to swap the reagent pack by another new one.
Trigger button
Replace electrodes: Execute all the steps in order of appearance in order to
swap one or more than one electrode. This menu must be followed in case
you may subtract one electrode for maintenance purposes.
Replace pump tubes: Execute all the steps in order of appearance in order
to change external pumps tubing.
ISE Alarms: All pending requirements appear when this function is triggered.
In case all is OK message ISE Ready appears.
All the messages in red colour warn

of pending errors.
BA400 EUG v 2.0 en
2014 Biosystems, S.A.
Costa Brava, 30
08030 BARCELONA (SPAIN)
Tel. +34 933 110 000
www.biosystems.es
biosystems@biosystems.es

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BA400 EUG v 2.

Any modification of calibrator Select the tests by

Calibration curve: Increasing

Level*: Numbering The option ‘Activate Quality

Reagent blank request

Select blank from the

Pre selection checking of blanks

Select calibration from Select Sample type from the

Pre selection checking of calibrators

Select control from the Select Sample type from the

Pre selection checking of controls in

Suggest a name for the

Note this feature is designed to

Go to WorkSession to restore it Load Session Select the session from

ONLY if there is not an active session

Select Patient from Enter sample ID in Patient/Sample field or

Tests Select Sample type

Select all the test to run

Check all your requests are selected in

Automatic Start: This feature allows, only if we have

The pause function is only available when the instrument

Working with results

AUTOMATIC repetition mode (Configuration → General →

Check the reason of

Maintenance: Special function that leaves ISE module out of

3: The instrument auto Calibraciones: Calibrate electrodes2

ISE Utilities (cont.)

All the messages in red colour warn

2014 Biosystems, S.A.

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