Use MegaCalc

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Welcome to Megazyme
To further support you, our valued customer, we have developed the Megazyme Mega-CalcTM to assist you in calculating the
concentration of analyte (as g/L or g/100 g) from raw absorbance data.

Use Mega-Calc

Instructions for Use of Mega-CalcTM

On the Mega-CalcTM page, fill in the orange boxes and it will provide automatic results in the white boxes.
To zoom up or down, ensure the Standard tool bar is showing (View > Toolbars) & select a value from the Zoom drop-down list.

1. Enter sample details

2. D-Fructose standard
If quadruplicate standards have been run, insert all
sets of absorbance data and the program will use
the average values. If less than four sets of data are
Sample details input, these will be averaged and used. The factor
(F) will be automatically calculated.

Absorbance values for 54.5 micrograms of D-fructose

Replicate 1 Replicate 2 Replicate 3 Replicate 4 Average Abs

Factor [=(54.5 micrograms of D-fructose)/Absorbance

for 54.5 micrograms of D-fructose]

Absorbance values Fructan Fructan

Sample Sample Dilution Moisture
DAbs (g/100 g) (g/100 g)
identifier weight (mg) (-fold) Content %
"as is" "dwb"
Blank Sample
1 1
2 1
3 1

3. Insert absorbance values for the blanks and 6. Moisture content & "dwb"
samples Input the moisture content of the
If duplicate samples have been run, insert both sample. Fructan (g/100g) "dry weight
absorbance values and the program will basis" (dwb) is automatuically
automatically use the average values. If a single calculated.
set of values are input, these will be used.

4. Sample weight
Enter the sample weight (e.g. approximately 100
mg or 400 mg) correct to the nearest 0.1 mg.
5. Dilution
If samples are diluted enter the dilution factor.
If samples are not diluted (e.g. samples containing
upto 40% (w/w) fructan) the dilution factor is 1.
Samples containing 40-100% fructan are diluted 3-
fold, for these enter 3.

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Further Support
To obtain further information about the
specific test, or indeed any of the Megazyme
products, please consult our web site.

www.megazyme.com

If you have specific questions, please contact us directly:

Technical Support: Megazyme Knowledge Base
Customer Support and Sales Information: Customer Support
General Information: info@megazyme.com
K-FRUC 09/19
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 Sample details M 2465
Instructions

Absorbance values for 54.5 micrograms of D-fructose Contact Us

Replicate 1 Replicate 2 Replicate 3 Replicate 4 Average Abs
1.2420 1.3820 1.3490 1.3410 1.3285

41.0237 Factor [=(54.5 micrograms of D-fructose)/Absorbance for 54.5 micrograms of D-fructose]

Absorbance values Fructan Moisture Fructan

Sample weight Dilution
Sample identifier DAbs (g/100 g) Content (g/100 g)
(mg) (-fold)
"as is" % "dwb"
Blank Sample
1 B3 - 16,2% 0.029 0.5980 0.6210 0.5805 101.3 1 14.5460 0 14.5460
2 B4 - 10,7% 0.008 0.4680 0.4640 0.4580 102.6 1 11.3310 0 11.3310
3 B5 - 0,2% 0.006 0.0910 0.0830 0.0810 413.5 1 0.4972 0 0.4972
4 2906 A 0.012 1.6000 1.4980 1.5370 132.4 1 29.4670 0 29.4670
5 2906 B 0.011 1.5610 1.5460 1.5425 137.9 1 28.3930 0 28.3930
6 1
7 1
8 1
9 1
10 1
11 1
12 1

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 Absorbance values Fructan Moisture Fructan
Sample weight Dilution
Sample identifier DAbs (g/100 g) Content (g/100 g)
(mg) (-fold)
"as is" % "dwb"
13 1
14 1
15 1
16 1
17 1
18 1
19 1
20 1
21 1
22 1
23 1
24 1
25 1
26 1
27 1
28 1
29 1
30 1
31 1
32 1
33 1
34 1
35 1
36 1
37 1
38 1
39 1

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 Absorbance values Fructan Moisture Fructan
Sample weight Dilution
Sample identifier DAbs (g/100 g) Content (g/100 g)
(mg) (-fold)
"as is" % "dwb"
40 1

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