Scribd Logo
Upload

Welcome to Scribd!

  • Datpeng

    Uploaded by

    Riswan Genesis Deadsquad
    29 views5 pages
    The document describes several experiments: 1. A test of the protective effects of various drugs against induced writhing in mice. Aspirin, paracetamol, mefenamic acid, piroxicam, tramadol, and paracetamol were tested. 2. A test of the analgesic effects of tramadol, piroxicam, mefenamic acid, paracetamol, aspirin, and a control using a hot tail flick test on mice. 3. A test of the anti-inflammatory effects of piroxicam and dexamethasone by measuring edema in rats' paws induced by carrageenan. Volume changes were measured at

    Original Description:

    Original Title

    datpeng

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    The document describes several experiments: 1. A test of the protective effects of various drugs against induced writhing in mice. Aspirin, paracetamol, mefenamic acid, piroxicam, tramadol, and paracetamol were tested. 2. A test of the analgesic effects of tramadol, piroxicam, mefenamic acid, paracetamol, aspirin, and a control using a hot tail flick test on mice. 3. A test of the anti-inflammatory effects of piroxicam and dexamethasone by measuring edema in rats' paws induced by carrageenan. Volume changes were measured at

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    29 views5 pages

    Datpeng

    Uploaded by

    Riswan Genesis Deadsquad
    The document describes several experiments: 1. A test of the protective effects of various drugs against induced writhing in mice. Aspirin, paracetamol, mefenamic acid, piroxicam, tramadol, and paracetamol were tested. 2. A test of the analgesic effects of tramadol, piroxicam, mefenamic acid, paracetamol, aspirin, and a control using a hot tail flick test on mice. 3. A test of the anti-inflammatory effects of piroxicam and dexamethasone by measuring edema in rats' paws induced by carrageenan. Volume changes were measured at

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 5

    V.

    Perhitungan dan Data Pengamatan

    a. Metode Siegmund (Induksi Kimia)


    Bobot mencit = 28 gram
    CMC Na = 0,5 mL/ 20 gram BB
    Volume sediaan =
    28 gram
    x 0,5 mL=0,7 mL
    20 gram
    Asam Asetat = 0,5 mL/ 20 gram BB
    Volume sediaan =
    28 gram
    x 0,5 mL=0,7 mL
    20 gram

    Waktu (Menit)
    Jumlah
    Mencit 5 10 15 20 25 30 35 40 45 50 55 60
    Kelompok 1
    25x 31x 21x 5x 20x 15x 13x 17x 11x 3x - - 161x
    (Kontrol CMC Na)
    Kelompok 2
    17x 6x 9x 4x 2x 1x - 3x - - - - 42x
    (Aspirin)
    Kelompok 3
    4x 29x 20x 18x 14x 14x 10x 5x 2x - 1x - 117x
    (Parasetamol)
    Kelompok 4 (Asam
    1x 2x 1x 2x 2x 2x 1x 1x 2x 11x 7x 23x 55x
    Mefenamat)
    Kelompok 5
    - - - - - - - - - 1x - - 1x
    (Piroksikam)
    Kelompok 6
    - 2x - - 3x 3x 2x 2x 1x - - - 13x
    (Tramadol)
    Kelompok 7
    - 2x 3x 5x 2x 7x 10x 3x 6x 1x 3x 2x 44x
    (Parasetamol)

    a) Perhitungan Persen Proteksi (% P = 100 – [[(JGu / JGk)] x 100%])


     Persen Proteksi Aspirin
    % P = 100 – [[(42 / 161)] x 100%]
    = 100 – 26,08
    = 73,92 %
     Pesen Proteksi Parasetamol
    % P = 100 – [[(117 / 161)] x 100%]
    = 100 – 72,67
    = 23,33 %
     Persen Proteksi Asam Mefenamat
    % P = 100 – [[(55 / 161)] x 100%]
    = 100 – 34,16
    = 65,84 %
     Persen Proteksi Piroksikam
    % P = 100 – [[(1 / 161)] x 100%]
    = 100 – 0,62
    = 99,38 %
     Persen Proteksi Tramadol
    % P = 100 – [[(13 / 161)] x 100%]
    = 100 – 8,07
    =91,93 %
     Persen Proteksi Parasetamol
    % P = 100 – [[(44 / 161)] x 100%]
    = 100 – 27,32
    = 72,68 %
    b) Perhitungan Persen Efektivitas (% E = [(%PU / %PA)] x 100%)
     Persen Efektivitas Parasetamol
    23,33 %
    % E = [( )] x 100%)
    73,92%
    = 31,56 %
     Persen Efektivitas Asam Mefenamat
    65,84 %
    % E = [( )] x 100%)
    73,92 %
    = 89,06 %
     Persen Efektivitas Piroksikam
    99,38 %
    % E = [( )] x 100%)
    73,92 %
    = 134,44 %
     Persen Efektivitas Tramadol
    91,93 %
    % E = [( )] x 100%)
    73,92 %
    = 124,36 %
     Persen Efektivitas Parasetamol
    72.68 %
    % E = [( )] x 100%)
    73,92%
    = 99,67 %
    b. Metode Jentik Ekor (Induksi Panas)

    Bobot mencit = 28 gram

    Tramadol = 50 mg (kekuatan sediaan = 0,2 mg/ 1 mL)

    Konversi dosis = 50 mg x 0,0026 = 0,13 mg

    28 gram
    x 0,13 mg=0,182 mg
    20 gram
    0,182mg
    x 1 mL=0,91mL
    0,2 mg

    Waktu (Menit)
    Nama Obat
    10 20 30 40 50 60 70 80 90 100 110 120

    Tramadol 15' 15' 15' 15' 15' 14' 15' 15' 7' 5' 9' 9,1'
    Piroksikam 15' 15' 15' 15' 15' 15' 3' 7' 3' 1' 1' 1'
    Asam Mefenamat 3.8 3.7' 15' 15' 15' 8.4' 4.7' 9.6' 5' 4.3' 5.6' 4.1'
    Parasetamol 15' 15' 15' 15' 15' 7.6' 9.5' 8.2' 14.7' 15' 12.2' 15'
    Aspirim 9' 7' 1' 4' 15' 15' 14.7' 10' 9.6' 3' 8.2' 6.1'
    Kontrol 3' 7' 15' 15' 15' 10' 3' 2' 1' 1' 1' 1'

    c. Uji Aktivitas Antiinflamasi

    Bobot tikus = 183 gram


     Deksametason = 2 mg (kekuatan sediaan = 0,01 mg/ mL)

    Konversi dosis = 2 mg x 0,018 = 0,036 mg

    183 gram
    x 0,036 mg=0,033 mg
    200 gram

    Volume sediaan =

    0,033 mg
    x 1 mL=3,3 mL
    0,01 mg

    Karagenan = 0,05/ 200 gram

    183 gram
    x 0,05 mL=0,04 mL
    200 gram

     Piroksikam = 20 gram (kekuatan sediaan = 0,2 mg/ mL)


    Konversi dosis = 20 mg x 0,018 = 0,36 mg
    154 gram
    x 0,36 mg=0,2772mg
    200 gram
    Volume sediaan =
    0,2772mg
    x 1 mL=1,4 mL
    0,2 mg

    Karagenan = 0,05/ 200 gram

    154 gram
    x 0,05 mL=0,03 mL
    200 gram

    Perlakuan V0 t30 t60 t90 t120

    Kontrol Positif 0,04 0,06 0,07 0,04 0,05


    Piroksikam 0,04 0,05 0,05 0,04 0,03
    Deksametason 0,04 0,05 0,05 0,04 0,05
    Perhitungan Volume Udema (VU = Vt - Vo)

    Vu t30 t60 t90 t120

    Kontrol Positif 0,02 0,03 - 0,01


    Piroksikam 0,01 0,01 - -0,01
    Deksametason 0,01 0,01 - 0,01

    You might also like