A.

Florence Test
Result: the semen turned into a dark brown crystal choline periodide
formed resembling haemin crystal.
False negative: low choline content or decomposed stain
False positive result: other body tissue (except vagina) may contained
choline
B. Barberios Test
Result: the reaction will depend upon spermine in semen, when few
drops of barberios reagent (alcoholic soln of picric acid) added to
spermatic fluid will produced yellow, needle shape and rhombic crystal
of spermine picrate the test is even positive even the presence of
spermatozoa.
- The limitation is pleomorphic nature of crystals

C. Acid Phosphatase Test

- A quantitative test
- The amount of acid phosphatase is estimated in a measured
specimen one square centimeter of stain material
- Give positive in old stain as well
- It will show a color purple within a short period of time.

Stained Material – it will show a purple color within 30 second to 1

minute.
Unstained material – it will show no reaction to reaction to the reagent.

1. Describe Seminal Fluid – semen is a fluid secretion containing

cellular elements, fluid formed in seminal vesicle and prostate, the
seminal vesicle contain high concentration of choline and lecithin.
 2. What are the characteristics and color of the crystal that form
under the microscope when the test reagent s added to the
suspected seminal stain?
- A drop of Florence solution (Postassium iodine, iodine and water) is allowed to
run under the cover slip. If semen is present, dark-brown crystals of choline
iodide appear immediately.

3. what is the substances in semen that is identified by crystalline

formation.
- The enzyme acid phosphatase hydrolyses the substrate to the
corresponding phenol and phosphate ion. The phenol formed is simultaneously coupled
with a suitable diazonium salt as a chromogen to give a characteristic colored dye stuff,
which is a positive test for the presence of a seminal stain.
4. What is the difference between choline and acid phosphatase?
- The actual pH varies with the particular substrate, but when phenyl phosphate is used,
the acid phosphatases have an optimum pH of 4.9 and the alkaline phosphatases are
optimally active at pH 9.8
5. Where is acid phosphatase if found?
Acid phosphatase: An enzyme that acts to liberate phosphate under acidic conditions and is
made in the liver, spleen, bone marrow, and prostate gland. Abnormally high serum levels of
acid phosphatase may indicate infection, injury, or cancer of the prostate.

Acid phosphatases are also found in nonmammalian species such as bacteria, fungi,
parasites, and plants, and most of them share structural similarities with mammalian acid
phosphatase enzymes. Acid phosphatase (EC 3.1.

6. What is the best evidence to show that there is an ejaculation

- Sexual assault cases are characterized by low rates of disclosure,
reporting, prosecution, and conviction. Biological evidence is
sometimes the only way to prove the occurrence of sexual contact and
to identify the perpetrator.
7. If crystal is formed when the is treated with test reagents what
should be the result
Positive depending of the concentration of the reagents.

Conclusion
In making this activity I realized that in every test that I watched
procedures and materials should be followed and carefully handled to
have the best possible solution.