Pharmacology 1 Practicals

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PHARM4oLOGY-
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9nDE Standard
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Sr.
No. Experiment Page Date of Date of Remarks
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Experiment Submission
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1. To Atudy the commouly wedutru-
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meu iu experimeulalphamacalogy
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2. Tostudy the differeit roulesof drug.
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admiuirhrliom nfor auimalAtudien
mice
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3.Praloal deign wuith
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o
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speial refereuce CPCsEA.
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.To Atudy the Conmnion laboralary
auimals
S. To Atudy he auaesthoic aguf ued_
Ju auinmal Aludy.
Atudy the effet of various drug
6.To
on rabbit eye uing Ex. pharma
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SDftuare
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7To Atudy the effed of drus nJoo-

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molkr dotiuily_uxinq Ex. pharm Soft

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ware.
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8To Atudy the vauia, pracedurea for

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rudening_auinmal wcomiauA Atuuin

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9 o rodeah.
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9 To study the dffeaive separion af
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Aerum and plakma,

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10 Td Audy the blod sampla Calle-

Hon AAmall Jaboralory QunMalk.
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Website for Free Notes & More :- www.carewellpharma.in

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Visit Website & Download Free pdf Notes

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xperimel to: OD1
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Aini: To siudy he co wocd Jusnuweuls Juu eperimentas paronia
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(alogy
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Reqiromu Orqan balh Rota rod, Amalqesionielu f photdmeter
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iecconvuiio moer, Coot's pole dimbing apportu,
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Roto Rod
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Actophotormclor-
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Page No..
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txperimetuo:- O1
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Aim: To
Aldyue_tonuuouly ueclJuiliumeu J experi
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meldalpuarnaCalog.
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Rafereuce :> udtazui S.k Houdlbok oexperinueuda puaanuacalogy
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Vallabla prmtaluau
4 editiou 6qe w
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KequureMOL:
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Orqau ball RalaAod.Aual geionuele-Aeu
pMntbiuel EecRs couNulaió mote CaDE'S pole
Clinbiug appaialiu
heory:+ Koa Rod : Oug de impOLau pharuatologycal
acus Í dueaud. auxity aqela
o beLgodiazepúue clau of duq
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usclee releiu properly
ue
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Acelstal muscle relastati toguluor uih taniug gfet

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TeLe aeulz educe auxIëY audowiou

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Ato patomeor:>The Jackometrk aaivtty Cau eauuly Meu

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Lurec UIg_0re adepliatomeler ulucu

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or cells ulicu aua Cuuectedl_

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Operae3 pliatoelectic.
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cisit wta_coudtulutu e beomoLiatt
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fallug c theplaatocet s W
of byike aunmal
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COatsecorded.-fAuaclopkatormele could Lave

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eelker Lcula uluc ulucu_tluo aunmal MOves

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Orqau bau The ue bath uand o_2u e auimal

tiue for zludyiustuadio _colled
Zludutorgau bat was diuiqued by Rudalp
Teacher's Signature.
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Orqon l3äl:
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A- ube
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OXYgeu
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blh
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Owex Oxyge
Jachcol is
Stirex
Thermaxtale
Rod
ELacrocoavulsionrozter.
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Plaquu w 1904 ue crgauball especiallyCouuickaT
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a). Au cuter acte_
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nmade upoff_ Atiel gla
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b. uter CYqm mada tup affglas utu
he
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om 10 m JSD mi
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_C).Juemmotadically cottraled uéalug od.
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d Stie do ceepue uaor utor-acreat ar uuifum
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temperiue
e)Oxygeu tiube ulidkaluo sorves atuue uolder
f Cua_coul,oe eud of ulucl iu Cowneond o ud to 4he|
Cõufaue uawiugplayaialogicalAalt saliulie.
Eleztro couvulaimelor u us apparaa eletinaloch Ju

ue
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appliediunugh curneal Teckoclos.
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ThAougloptirAuimuletimcorical excitalion produced.._

DiES)_LmaximMal elecuo-Auock Cauvulioul are devided.
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Tue
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UMo five phausuch

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aa. auic-filialion
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b Tnuic etoui
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Clouic
ClouicCOuvuliov
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d upe W
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e. or doatl
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Recoverivg2
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Cobks pale climbiug apparalis 1k la employed torthe

Zludyof Outiseptic cuug.
t a ued to udy coquative-functio_Mauty a

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Cook Palo
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vi
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Cimbiuy Appardut
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Cedritug
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Aalgeriomele
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Rexul laboratory appliaert

ed u he pharmotaJo9y uwore
Studied 9 ulere
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ebU
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rApaueJo Couditio Junul dluu? Joaviy_audib
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cm
apparas has au eipeimouda
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LT he lorgid a sod ptooi ntlosure.-

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Coatuifuge: Rapidly ralalug) Coxaiior that _opplie_couatugalL
ofur_perpeudi-
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Aepaude
fone to ita couleutdrpically
-fluid anus. applyiug2 a
spaiu
paudiallyIhhin
CulaTb aleaof
Aualqesiomelir:
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Rapid aud prèikesueg aí aualheic. cluriug2 poperties
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Small Jaboratorie auuimali.

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Orid iu Lat plale eAualgesionTalir has beeudeveloped

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or lasiug ihue plaw, -

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ua o a MeniuremPL oiT aumals rauitauce.

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trourparel
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Result sue dahoralory applinueo ued u due plharnia
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Audied
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Coloqy_ulare
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Teocher's Signature
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txpenieul uo.02
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-fim:To Atudy e diffedh rouWe df drug odnuuwh alin Jn uce,
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nuce
s Hopdiuo
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Auua pantoues
adniuixtrin
of dny
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txpenret vo:-02
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Aim o Aiudy
e differCut rauo o dug adniniulaaliu d
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nice
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Keferuce!> kulkau St Nallali Prakauau uaudbt experinuaal
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puamacaloqy ane uo
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Kequiremeul:Auinal Dlice geliM Cauda, elc
Priuiple:> Tue e_aud duaioa adioL of_ duq depeud aw

oule off adnuuikfias, e.q. ullkeua druq giveu
byulAveuou_ule le effec s AuL atareucuuly cr
Compcuud to_cral adnuuutaticu off druq uleu ake.
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ipto 1 uuL Awuw
le effec.
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Route: 1).Tuoveum
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2). xal
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3. Tuapesidaueal
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Proedure:
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aumadsQud uun»bered uem o

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1)._ueiglut e ueJail.
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2).Dividee acn> uto dlueegrepe eacu ofCouuULing 5 nice

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for uile peidtaueol.iutiaveuous aud ora oulesaf

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aduuitati
e
3Tukaperidouealiujeilim umade obdome uulkeue
*
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(
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Ora adiuuralian oí druqa
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uluaneuou adnuuuhaim W
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ot cru
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RexultThe differendOul ofdruy .a.dnuuutrabon M

nicewone udied.
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a tue doraalJau
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N _used for dicNCLGL adnuwa
ora mouuo by
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tia for roule uald Le Jkucc nnuclk auud
ucedte o
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plate uefecdiug Le douque audqgenlPUs
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thusAaltica u ha moulh
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).NatRie Jlu ime c dujectio aud time of aet afJeep.
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u
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tuuieJlue aumal Aleeps._pu uutgu backAo uat ukein
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t_regau ccuicuueuit uult oyer Jo uunnad
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poime.
5 Nate tuo tinne ol recoveN ud calculate dho_duialian CT

actio
6). Compare dlue oT A actia aud dtuatia ot_AlLep_duc te

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piusborbital radùum uhengivo by dffercut rOule af adniuw
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aticn
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Result:Tue ditferet rOulo of drug aduTtiatiau nuce

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ulere iudied._
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Ex peimAdnDO?
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Fefereue
Pratsca deigu forauinat Tuclies illpecia1
fim
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Experimout uo
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03
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Aim:>_Palora deiqu for auinialtudiu llApecial rqerenee
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CPCSA
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RefèrevuLe i> cultaui S.. uaudbaok dd expeumeulal _pharnia(logy
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publied by allabuprokahau uh edticu 2012)
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TheoryCPCSEA: Comnittee fer
Auperviiouo
ue
purpoic ar
CGUdral auel
experimovitk ou aunmals
Tu Lomnuittee ha differud aplicaticu i auimal proure-
nmeul
Aumal procuremout:2-AU aumal nut be aquired Jawtulle

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porhe CPCSEAguideliuO
Atcallh
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suvellaureprmgram forAcreeig uconiug)2

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auurmalsuould b0 cauied Cutto aieK auma auali.

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QuramtiuO Stabilizaliau aud Separatiou> Ourauliue i

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ueuty
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Beparaünoi_
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recieved auinad frm uM aliedy u_uefacilitup Ltil_e
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Lualtu aud passiubly microbial Aldu of he uCul recievee W

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aumals have beeu olatemuuecl.
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Auuma care audTecdauica Peraonal Nunat Ccal p2auanwu

requireochuical auel
uubaudAy Suppo ALDaLal migld omploy peopla Ier
baLle fomal aud djob aiiug? uue
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effeative umplomdalion oÍ ue_peg.an
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fuma Experimeudtatiauuvalviug Hazarcdau ayei :4 1uidiui
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Auould uav
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paioe cenuua experimeatati h hazardou agukTatilu
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iaal Committee Llleso member are bucuuledgahleabout hazoj
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Jduagulk.
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Duriug of epenmezt No oumalAluoudd be ued for expeinieu
lalicafer more fuan 3 ycar ltss
adequale CTficati pmvided.
Puyaical restrait: pluyaical_resltai er

auinal for exa-
a
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miuaia Callectia of samples aud
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Vaity of cluer cliuidlaa,

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Fudlianal_areaM:> She aud Latur ot atacility ui difer-

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uue wnatlerarea to Aeparale Aenice

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functiau arcpostuble Cr uecceLor

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Puaical Faciiitie:>
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Kuldiug hould _beAoledecdto facilitale cfiieut
mettnal
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yqieuce_Operaio4f auimaly-factitie,durable.
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aul
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maictuc put fire reÍKlant,
oImdorc slLculd bC uidc uOugldofacilitale s

mavemu
Teachers Signature
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Page No.
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iiiesAudh as ualer iues draiu pipe aud electneal cauno di
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ould retarabl be acceuble lucugAevice paues
paels
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iMauls room_dorsshaudbe utvemiue aud du prml
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Exterior wuudow are uat recommeuded fur mal oumal fad -|
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itig.
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flourMuld be n
moliureprTot audAlLA_pratL
al Auould be freeof Cractes wealed,_
EuviDuMeut:
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Tempratire ad Humidty Codrad:Tdeay Capabitity Aueld be
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povidedto illow vaiaia

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lu uthemuge otapprmx 18°C 29C

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Veuctilatiou: Tu or u buildiuguew
reuOValiug exictiug
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Oimal-facililiaCouidratisusold 2c aivCL
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the Vedilatian of she auinmQb_primary utlosurs.

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Noue Contini : he facilidy Siould be providod itle uaue

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ree uvioumo uoue (OTulkaduimpataut
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CCLLSideratta udosiquuug/ du auumaly facility,

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Aumal Hubaudary :>
Cogiugor housiug Aem 4me caqug aIom ea

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mbztuporlead elcneu
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|S0cial euvironmd":> ueoial eUAOumeud ucludos all dc
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uuteradkiau amouq udividual c a qaup
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u-
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Fwd :>fumasAuould be fed palatable (outamivaled
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aud uluilieualty Odcguot
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Waer: Ordiuasly umals Auould uave Cdiuos acceto
fres patable Acaufomuualed_diitiug_tuater accar
duua ueurparlicular requremau,
Stoudardoperatiug_procedures Guideliuek2eutuke Aual!

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maiilau SoPz des
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crilbivq procedures motuecla adopted ll eaarcdto animal

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wsbaud
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aud-Luthauaia ia
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Auaethesia
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Anaertheia Uu coüliauy
da he aclúavcmruul_T ile
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Yesulty DZUd eclalive ualguic aud
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Quodheia
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Eharaia :> t Ju euorted tlo veuli tare Q auumal

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requiredto be sactiÄtd outom-=

zation of experimou
Bree diug aud TMgic For uiliatu a Calouy Hic

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breediug Stockmul be_ procurcd rom CPCSEA reaulered
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Breedor a Suppliera. ALuiug Lat geuolic malce- up aud_
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uealt Tláluof_ uimal J Cuoww.
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Result:> CPCSCA qudelue fer auumal ud ua beeu. Atu
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du ed.
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Teacher's Signature :
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txperineul uo Oby
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aumaa,
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im:Tosucty he Ccontniau oboraory
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fro9
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Experimeuluo. O
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Aim: xIludyLie Comia_labeadai auumanls,
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: Di liniauu jasti -xpurimeulal phaniato.lugY ubliled
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Koferemce
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y Di_depliTti 5_cditnn 201
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LO.
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Requremut plouceSuespDog2. cat.RabbilCueiea pig
Ra nua ro HmiKI
Theory:-irog? (
Raua tempara Raualiauiua )
Cuaractoxia
Auamplibiou auimal
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aud Saflo ue
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Fay
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uftiult breed ulaboralory

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ExpenmÓt TuAe :2
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To udy1ue duG adio M Cevtlral Nernou system CNS

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Neure nuculau AWAliam (NDI) aud hoal oteT.

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reciy abclmaiuús muca.

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Istaedtue prepaiaiaie
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lAolEted lucaprepataú gagiauinuwsAtiotic uerve prepäralie. W

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Taricity Sludies ReTinatoualy
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Local-Auacsilpic
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louse ( My- musules )_
Characdernatics
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MOe
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Datc
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Nory Amall Uu AIne.
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Ccounuic ud eaiy lo uaudle aud maiilaiu
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Deied romJue _uild mouse mus mucudus
L
Seusetivele aue Lieaniuq_uth wiueUife Apan
E
E
W 1' to 2:
W
years(rilatio_period 1921 daya
E
E
Auragesize_LLuULe]S_6do 12
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R
Body lomp. 36- de 30'
A
A
>325 o 80 heat Inuuut
C
C
HeaclFale
Respuclory rale O-01 0:23 ml
Expenmufal u01
Rio auay Jo duliuL
A
A
Screeiga aualgeic aud ulicouvuliaut
M
Screeuiug o cecm0Llerapauic aqulk.

Caucer -ESKardi aucd qeuuolictudi es.
R
R
A
Study of cdrugs alug C CNS)_

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Pat Rotu ueNigicsI

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E
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Characteritiu:
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very activeu dark or uigli bult vory pabr u day igtd
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A
Imk uuthIue
thetuitoy
C
Small uu siza [adult ueiqla 260 lo 25Dgm] Meuce mall quanlity |

tut Aample.
conue iu abiouut
Vamiting
Respisclony Fati HoTo 11mivte.
Teacher's Signoture:
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Page 22 of 47
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Hanuler
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Page No.-
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idal Nolunie 0.Gnl
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:
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Expenmelal_uMe
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Toucalogicad Ttudies-AateAub- aqule clrouic aud orgau
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E
Honucdy atudies.
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e.
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A
13i eay Ti Mormaua OXylonúu baic.
C
C
Beluavinu. aud purcltupharnuacologicad Tudiey
Cttect of dnigs 0u bua blodpreiure
HamateIA
Charateritti a>
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A
M
SuOctail adutt weiqlat 90to 90 qm

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tett clevelepeclAouue af
heuiua aud smallL
A
feu Toes iu fu Limbs aud five taeiu kiud imbs.

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Life spa
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2-SYears.
Terlaticaperiod 16to 21_doya.
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L
L
Omwvasous
E
E
Ceprophagic
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E
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we
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Expenmud
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C
Repm dudlion aud eralsgeuicty xtudies

Piocelfor diab mliu3
Sudytue efect of uypotuennua
Poas tarch elaled to lueimmuralogy caucer aue joLvalogY Suclies
Teacher's Signature
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erntz.4AEARn
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Cruimea
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quuc viraloqy
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| Bio auay of prosaqlaudw
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E
E
inea Piq [cavia Parcellu)
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E
E
Cuaracterivics:
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R
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C
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aA0cialAocial aud dordil Lumalladult weiqtd ugato510qm]
swiqludyAureptiblelo uberculoru
Ctonac uedirely qlaudulau.
life spa 3to S Years.
Creaiatiau_period 6 dayA
Bocly teomp 312 do 39.S°c
Heaul rle 23o o 360I niulk
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M
Herbevorows
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mnnuolegitalludie
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Evalualiasaí brockodilatoa aga experiniedal_uduced

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ustlhma
io auay
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E
j.e._cigudalu utaniue aud
_ eobolim.
W
Studyo lubereuloA aud Acreeiuy aauli-tubercula clruos.W

-
E
E
Sudy ai vilanún
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A
or orycalaqu
C
RabbitLhapak Cui luL

Charácteistica:>
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AN
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Robbit
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ResuulTe Comniou Jaboralory auinuals WRretudied in
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puarmacology
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Docule auma-Adult uciglki 1:S3.0 f
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S19 as Clha earvesso
eal for blaod cale
cuou
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ulo 6 ycas elatiau peiod 31 dap
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GkApau
E
Kocdy lemp_ 38 de 39-6'C,_uclalc 130 To 325Imuue.
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W
Reupiaory rale 32Jto Go luuuk
E
E
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R
HerbiVOIDu.
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A
C
C
Expemata we:
RnqE lerliv
Bioauay al druti- cliatic aud sinu LuarmaueL.
Screcuiug at aqeul aflecius Capillary_permeabiliy
uuere Aludiec
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A
Reault:Tue Comnio laboratoy_auunalk
M
e puammacolog.
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Teocher's Signature
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ry
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xpelnant nb.OS
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agul wed aumal
Aim3To Jludy: theanaerthic
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tudy .
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.
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E
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=
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Expenméed uo, ops
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Aimi2oAudy e auaestuetic ageul ued iu auintal ude
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L
E
E
kefereuce : lul kasui S.E.A. uaudbaE af expeimuial_pharniaco
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W
Jagy allablh prakeslau -fditiou
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E
R
R
a ac
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A
Theory: Auartueia af poVidiugAeuaion free
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C
relief from Pau
SedatioiaA_mild otceatial depreti o6

digree u utliclu
e padieud u auake bu calmlouger_clade ot
Aecdalive may leacl to uecroAu
o te
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A
Lecrosi i Adrug uuduceel Alate sadatio ulic
pau.
M
-paieafeel
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A
Auaestueaia are of twotypes:

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1.Local Auaestnesia : of SEuiaia ualinitedbocy arta.

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rocaiue iguo caiue t.

L
E
E
W
2)Teueral-Auaexthesia : Complate ucouiouueu W

E
E
eqDix tlnlettuor kelbniue
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R
A
A
C
onumauly used (aberatoryAuastuesia
EHAyle (orboniuo
Peutabarbita
Teacher's Signature
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3) Phenobarbital Sodiw
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uParaldebyde
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s)Icolomiue uydrocllaride.
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1).Etyle Corbomiue T eodilrAalubte u ater
: Hralaluliou giviLug_a uC
E
E
2S at salitau
uAuaily uu
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A
A
unter ued.
C
C
Dog
Dog 25 at aqsaliliou 11-Sgp iv
Rat > 90. of a Aaliliau / 1.Sq ip..
Rabbit 2sT salülio /0-S-1-3Sq iv
2).Peutabarbital:3 Ws abarbi:luale auc nibt conuneudy ued auae

uaticu rodeut Coue_puuinmalCardiovaular
A
A
cleprewicu.
M
uJoug adtiugArgical Cuaestlatic.

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Dog aud cat Saatiom30do Sdmg iv orip

A
Ra aud mouLe: 0.6). Salulion30to Gomg iv.

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H
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3).Phenobarbilol Sodium:2 11A ued for prmlaugelexperiment.

L
Deg oud Cor >101 180 tn 2g0 ma ip

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agAalaiaL
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W
LParaldebyde: W
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E
R
Advaatage : TN.iujeclia
cliaulikel to procluce cadiac
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A
C
dilalias Qud pulmouauy CoLYecliou. Lcoulractiou
Disadyaztage :DogTt iat.Luueu

61
ho 8.P
salutdM 1.2 nd ip
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Etans
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Cal 6. Satülic 2:1 mlm
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5)ktanuue_Uydroculoride: ILdisruplllusLpau hauniiic
E
E
W Qud supiesApiualcorelacivity.
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E
E
Resull i Te auacatu.aticageul wed u_auimal dudy
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ulere udied.
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xpeunw uo- 06
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eye
Jhe eflet of various druq Ou rabbu
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Him: To study
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IMg Ex- phaum4ottuare.
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E
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C
C
Kquiremont Rabbit. eye droppe, rabbil uoJder drug Aaluiioa
0-3 7 Adenalu. 27. Puysatignuuue. 17. fAtropie
amdl 17. phedriue
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Page No.
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Expenmui uo: 06
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Aimi To study ue efled variou druq au rabbil eYe _uIug
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ol
L
EXplham softtuware.
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Rcereuce i> ulkuui C.E. Llaudbaut attpennMOudal _pluarniacola-
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E
R
R
H editi Vall ablu_prakaskau.
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A
C
C
Requremeu: eye droppera
Kabbit rabbi Lolde drugAaliücu
0-1.Adreualiue 21. Puysastigniue 1/, AHro -
piue auel 11Epluedriue J.
Theory:+
ae ued far tueir

A
A
Pruuple: A large Lumber i drugs
M
docal cian d e Rye a ye dopsroupsioxx

o
R
ot eLe_ drugsbeloug udoumie oups.

aulimicarobial
A
Ihe eye Aupplied bllu para

by AXmpualluilic avud pra-
H
Sympaluic uenves
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Tue ciliary mucle A alo Aupplied by lhe_parasympa

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L
ueic uerve ud uleun t couinl Hhe_ciliary lbody

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E
movec uutwarcds ud uruards
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Lulueu te pupil_dilalu tuieru fullY bach Mear tlue
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E
o Ahlomu_ud craiu age o
aqecus
R
opeuugtho Cand
A
LuwmourA cicreased uereby Acrea Iug udrooccular

C
Ltetaeu s cleeteated preAMure.

Coustriaioof pupil by oppastlaActios
h uill ucreasing
draage aud veduce ufralar presue.
Teacher's Signature
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(oawd ofor
E
Drug Pupiullary size Siqul reflex
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Pres eut
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E
Saline Nornial Preol
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A
Pyaarligiue(nutriction ret Pres u
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C
Eptaediue Dilatia Present Pre et
Atrpluue Diuaion Abrut Abred

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Pesuldhe effet of vavious drug a Rabbit eye

iu
R
eo
A
Aoiítuwart ulasjudi
Ex phem
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Page 35 of 47
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Proedure:
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1).Platetuerabbite bou ceepiua he heac uAide,
E
Observele size o_pupi
W u ballu uue exes.
W
Examius eeye efot of Jigld reflert by haldiuthe tordh ufiot
E
E
O c
R
R
nmoviug iglui beam to aud fro.
a
A
A
lulil fu dropsof autkopliue, Aal, u
Coujuctian over pernod a
C
C
o 8-10 muuull igld rigd eye of
Hhe rabbi aud leH eye's
rabbit dould AeNe a Coutrol
Kocordthepiupillan siza _ligut Fel,lex _aud corueal refleu after
Lnuu o drug wtuUatioa audtabulale obaervato
Repeat o expeimout mk playAasligniue_aud epte driue
aud fuer clrug
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Result:The effect of yaliou druq o tabbit eye uiug2

R
-Ex pham coft uare uas zludiedAucceufully.

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Teccher's Signature:.
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Page 36 of 47
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txperimeul no.07
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Aim: To udy Jhe effecd of druge oAJoco rm
Dir aivpb
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UAin Ex- pMasm Aofiua.
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E
R
R
Obzervauoue
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C
C
CLucuege i ativit = Pror drug(doconolor aliinPot dnugx1
Pr rug
S.uo | Daxe LocomOt1M

Paxl druug
Chauge
activiy
u
fre-dru
A
A
Veluicle 13031 26
M
2 Smg ta 13S 64. .8?.68

R
3mg F lo.02
A
3mg 6
H
12.+83
P
S 3ma
L
L
L
E
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E
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Datc
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Expt. No..
Page No.
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Experimut un:a 0t
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Aim: o tudxue effectof drug ou Jocomeler aduyu
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iup Ex- pharm Soltare
E
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KeferuUce cullcaru SK, Haudbwt of xperinieudal pharniacology
E
E
edittic 2012Nalab Prakasiau.
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R
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C
C
Regurmeuts Aumal : plice
Apparatu Aatepuorme, drug
Theory :
Priudple DTl of (NS dia aflect Jo cametor gdivibe D
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A
M
1). timuaubAA (dffive ample, amine tc.

:
Rarbitutalos alcuoa t.
R
2)Deprameuds
A
Lecometer activity = ude of wace fulueu.

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H
P
CoLurtructiou:
L
L
L
u
E
E
Sin bui- pkalo diode
W
four digit digilaldispla W

E
E
Couduicliw1g rocu -for ilucorpoFatiug elecric sluock of upto
R
ra.
A
100 Va for adivtiuy_

C
Jalorciug Gpre opërales oM pMalo electric cel0 Couuetted

Lu Uruul wwtu aCouuler
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Page 38 of 47
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Date.
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Expt. N0.- Page No0.
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Procedure:
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1).Luleigbtle aLunialu aud uumber tlem
E
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2 uM ouulue Rquipmau aud make Aure all PTclo cells ualdui
3Place udvidual cage
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E
mouieLu_activüty tor dipuläled ime
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R
Qud aud uato e baal activity Sme for al auinnay.
A
A
). 1uededtue drugchdorpusonaziue L doxe: 3 mg l1k.p
C
C
S).Calculate /. docreae u malor activity.
Result:>Tue plecdofdrug ou Joconiator aclny iug Ex-

puam Saftuarg uaAtuclied AuCceriully.
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txpeimnt mo- 02
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}he vanOuA proceciures
or reuderuiug auimal
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Aim i To ludy et
otrod
L
L
wCOucIous Atuiug
L
E
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E
E
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Page No.
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ExpeiMeul uoa 08
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Aim: To sudy ue VOiOyprocedures forFeudenug: aunual
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UMCOuAiou suiuy of rodeul.
E
W
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Refereuce :> kud karui S. Houdbmk of -€xpeimézdal Pharniacaloc
E
E
eciticu 2012
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R
Vallablu Prakasua.
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A
C
C
Theory 2 Uiug?_Chenuica aud plyaical eudhauauia
Ethauauia :s Itu tho at ot ududiug unau doatu iu a

QuinmalAacrificiug -the expenimeulal ouimal after
after
Le by geulasprocedure Coauuiug miinial of pysica aud
Stenug Callec euulu auauia
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A
M
Muthods:uere are kof tuo_motluoda..

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R
A
1).Cenical: Tuhalou reaqed- rLer ualonatertumue chloroforn

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L
Tujectable_ageud g Celantiue Chorouydiatel

L
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W
2) Puyaical i_ W
E
E
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R
A
Cervica DiloTaio-
C
Decapitatiou
ui
PiHLug
Teacher's Signature
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A
A
H
H
Cerviual Duloaiau :
P
P
L
L
Advailage : It
L
doesLiat chenically coutanuivate liue uis
E
Tecuuque luduce rapid Jau of couuuauueu4.
W
W
E
E
Decapfatiau: T cau be uued for rodculk aud Anall rabbit
R
R
A
A
researchotf iuqs.
C
C
Advazdage : T doe al cuenically coula ceuau coutaniviate
uueHis techuique udute rapid Jau lka of
CouCiOuuuos.
Duadvaudage: Tustecluuque iy bo dutreutul to_cuunals.

A
9 Auual ma be uuod a
M
Stuiug by blow lo the uead

By te ue of
R
u-_peLelraliug captive hald

we O
A
by tha electicCurret
H
H
P
Pituiuq JJu xed for procedure Jo euuure dleatl af

L
umal uad Las beeu

L
reuderec_ cOuuuQus
E
E
by aluer meau
W
W
E
E
Rault The vaiou_procedue for reuderiug auinual u
R
R
A
Coucio ATuUug olrodoulj xludied.

C
Teacher's Signature
A
A
M
M
R

A
A
H

P
P
Page 42 of 47
A
A
M
M
R
R
A
A
H
H
sexum Ond plama
etfeive Aeparatinn of
P
P
Am To study 4he
L
L
L
E
E
W
W
E
E
R
R
A
A
(Oulaiuiuo
cOulaiuiug
KPgvintmort:- Hume blod Aample, Naruouer ube
C
C
:appropriad
i cauguwa, Aeradogical pipoleu of AuAiug aud
Valume,Crywal bncldop cntfuge
,
rolvr
A
A
M
M
R
R
A
A
H
H
P
P
L
L
L
E
E
W
W
E
E
R
,
A
A
C
:
A
A
M
M
R

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H

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P
Page 43 of 47
A
A
Datc
M
M
Expt. No..
Page No.
R
R
A
A
ExpennM d uo:a 09
H
H
P
P
Tm:3o ud uus atfedlive eparalic o srum aud
L
L
plamia.
L
E
E
RefereuCL : Prulocalu for_prepaialiau
W of blbud
plainua Cud Serum
W
WWU. au -Org. com procedue lorAeparaluaia_plauma
u
E
E
aucd_Aerum
R
R
A
A
C
C
Requremany _Humau blodamplovauutaiuer tubes CotUUIg_
auulicoaqulau. seralogical pipetteal appropviao
Valume Croviat beucdalop ceutnfuge ulh_suIug Aud Yalor.
emy Blmd planua ueiquide iquide_compaueu of hlnd i

mluichtho blbcla ceils are supudod 1 mates
abou
A
A
o1 of dotal blmd_yalunie C901_udCouiai
M
dimolved p/aleius glucae_aud nuueral ious

R
R
A
Seum dL cleatueu planua of fetwer protiesprotieu ave

H
ACme Cousiderdasuderjaciug subtauces AoMe_

u
P
HelsaA uey vead

utl veaqed audMere by ield uu
L
L
L
accuFO rOSUlts.
E
E
W
Procedlure: W
E
E
R
R
A
Separatioa of pluma
C
1). Callectue blodJd puple top EDTA tube aud cordni-

uge 206D RPM2 tor 20 nuib.
A
A
M
M
R

A
A
H

P
P
Page 44 of 47
Date.
A
A
Expt. No..
M
M
Page N0.
R
R
A
A
H
H
2).NHrCeidbilugatio UKiug
tleau npelletecuui.qu0lace
P
P
plainia iuto :S mi-Eppeudro ubeJabelled uillu.
L
L
LLactiugLLumber
L
Ludplaima.
E
W
W
3).Tecze ummediall d eoC u freezer.
E
E
R
R
B.Separati on_df Serum:
A
A
C
C
1).Called 1ond ulkale bladcuiugseum Aepaater ube fscTI.
2.-ALlouw Aample to clot for1 uour_a romtemperlure.
3CelHfuge for 10 nuud appxoxiniadcy 1000 RPDi.

A
A
M
u)Usiug cloau_pipette Jecuiquoaliguat 210 UL ot. serwmÚuto

R
Aobelled Chyovial.
A
A
H
5Tmmidcall -freezeviak af serum d Go'Creezer

P
P
L
Result: uesepataliou ot_Arum_aiudplaina ludied

L
E
E
Auccessullkp.
W
W
E
E
R
R
A
A
C
Teacher's Signcture
A
A
M
M
R

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A
H

P
P
Page 45 of 47
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A
Expenmeul uo:- 116
H
H
P
P
tie btoocd Aomple Collecliu JA Mad
Aim: Totudy
L
L
Jaboraor auumali
L
E
E
W
W
E
E
Observaioy :
R
R
A
A
.
C
C
Ainial S
uart :Dtedium Lou
SDeciea Auutheia Anethesia, Auctth eaia
ice13 Lsottrauce) Xylazauet Felanune | Xytaime+ Celanar
Haleitioie4(mgd 16omg urm)C16mgt6omgimlip)
Rat 14 Yyleriuue+ Kdanine xylovnet Lelan

(smg+16omg um) |(16mgt60onmgumlir)
A
A
aafulaoe eriwe 4+tanmelaue+ölauim
M
Cuiuea p1
(ualalion) (2mgt 8omg um)(umg+1omgu
R
R
A
Rabbiy Jaatulanauce Xyloines kolanuze Xylazie+ kelari

H
(uinalatian) (Smg+IS 30mg ym)Smg +tong.im)

P
P
L
L
L
E
E
W
W
E
E
R
R
A
A
C
C
A
A
M
M
R

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H

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P
Page 46 of 47
Date. ** *
A
A
Expt. N0.. Page N0.
M
M
R
R
Experimut uo:a10p
A
A
H
H
P
P
im:> To tielyue blod amle calleTlin uuAmal Aab-
L
L
OraonN OLLu mals.
L
E
E
W
W
Refereuce Para Surumau, R. Raveccelrau aud R. vera-Nad lacH
E
E
Aample callectiau u mallaborator aumal,20t
R
R
A
A
Requirema: Auúnmal,_auaothetic aqeuts cla gloVeSur
C
C
qical hlêde blmd sample Callecliou_uubes veedlo
Capillarube.
geueral_priuiple of blood Callectiu

of bloocd Coleclioushould be discibed u
A
A
Te nieuUMocl
approved by ue Luaitute_ auinnal ptlic
M
he pratoca
R
Comnuttee.
A
ILAuould Jeartpaulul
he aud siresrfull.Rlocd ample
H
may be coleted uder auaetuLia.

P
Aclequatetraiuiuq Fequired for hlod circulaticm uiug-

L
aUy_nmeiLocd J awyApeuies.
L
uuthdrouiu trom veuou art-

E
E
Tugeueral blmcl Aompleds
W
eia blmd veueu ou ueut cluamber. W

E
E
CareSuould be talce fo alder aud obeie auina.
R
R
A
A
C
Procedure:
Afor dlorsal Pedal Veiu:
Teacher's Signature
A
A
M
M
R

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H

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Page 47 of 47
Datc
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A
Expt. No..
M
M
Page No.-
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A
A
1.Tue auinual u lecep ul a relraJne.
H
H
P
P
2Hald the luud fat around Gele+he medial clorial_pedal veua
L
L
u dotarecl ou tap of the fool..
L
3). Cleauthe Foot ulith_abialule alcholuol
E
auud eutdueLe_clorAdS-
W
W
pedal veiw _uutlk 23Cr I2
CueRdle
E
E
R
R
BforTau vuiu:s
A
A
C
C
1| Cepl
eouumal u_restraiuer, tenperalure [2uto 21°C
2 DoT6 Hub heTailit MCLy_resull uu_leukocxtais Tt Hhe
Veiu d Co vuible heu dip the tail u_wam watur[uo'c
3 Kefore the 3onivntey of expurinioutal apply Jocal auacttioticC
Cream ou tail.
u). uAer ue 23 uee dile unto_blod Veuet auedcollect ttesampte
A
A
capillary tuhe.
M
ing
5 asu tlueeraiuer to avoid CrO yeticuu.
R
R
A
A
H
Fortail Suip Louly for nuicel

P
P
L
.Apply Local auaciheuia ou tal.

L
a
E
E
2). Make cul of 1 mm froMipof lail_Acadpel blede.
W
3) Callectue s0m(le. W
E
E
u) top the blmd fAlow by dabbiug? uedau dip
R
R
A
A
C
Result :4The blod Aample Callecli uAMall JaboratorY

auinal uuk siudied success.tull.
A
A
M
M
R

A
A
H

P

Pharmacology 1 Practicals

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Page 1 of 47

CAREWELL PHARMA - A FAMILY OF LEARNING

7To Atudy the effed of drus nJoo-

molkr dotiuily_uxinq Ex. pharm Soft

8To Atudy the vauia, pracedurea for

rudening_auinmal wcomiauA Atuuin

Aerum and plakma,

10 Td Audy the blod sampla Calle-

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Acelstal muscle relastati toguluor uih taniug gfet

TeLe aeulz educe auxIëY audowiou

Ato patomeor:>The Jackometrk aaivtty Cau eauuly Meu

Lurec UIg_0re adepliatomeler ulucu

or cells ulicu aua Cuuectedl_

COatsecorded.-fAuaclopkatormele could Lave

eelker Lcula uluc ulucu_tluo aunmal MOves

Orqau bau The ue bath uand o_2u e auimal

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Eleztro couvulaimelor u us apparaa eletinaloch Ju

ThAougloptirAuimuletimcorical excitalion produced.._

UMo five phausuch

Cobks pale climbiug apparalis 1k la employed torthe

t a ued to udy coquative-functio_Mauty a

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Rexul laboratory appliaert

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LT he lorgid a sod ptooi ntlosure.-

Small Jaboratorie auuimali.

Orid iu Lat plale eAualgesionTalir has beeudeveloped

or lasiug ihue plaw, -

ua o a MeniuremPL oiT aumals rauitauce.

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Priuiple:> Tue e_aud duaioa adioL of_ duq depeud aw

aumadsQud uun»bered uem o

2).Dividee acn> uto dlueegrepe eacu ofCouuULing 5 nice

for uile peidtaueol.iutiaveuous aud ora oulesaf

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RexultThe differendOul ofdruy .a.dnuuutrabon M

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5 Nate tuo tinne ol recoveN ud calculate dho_duialian CT

6). Compare dlue oT A actia aud dtuatia ot_AlLep_duc te

Result:Tue ditferet rOulo of drug aduTtiatiau nuce

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Aumal procuremout:2-AU aumal nut be aquired Jawtulle

suvellaureprmgram forAcreeig uconiug)2

auurmalsuould b0 cauied Cutto aieK auma auali.

QuramtiuO Stabilizaliau aud Separatiou> Ourauliue i