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PAGE NO. 56
xPERIMENT 4
AIM
lo SHud
actian salivaAy amylasu ou s tazuah
APPARATUS
REUIRED leat kubes, tat ube Stauds pipettes, buk1n eotton,
nne walen bal Spiit laup o
bwun aum
eiondu, stasew Sodiuw chlaidedisiued watr iodiuu
Seluticwiodide, the.
THEORY Salivary qlands1Lut iu ueuth eauit Seuxete Saliva
T saliva coutaus, mueun to lubicoti 4sod aud_a s tancla,
diqtakng Mzyme Salivay alaat
2 Saliva QAylase acs m stanch.,a polysatahamde aud
chauges ik uth Simpl sugas
3. TMeAZM u t aetive at leWrakura 93e aud ak
pH G8
4. Like 0Hara _euzymA, Sakivonms amua is alao ettnae
eous1'u deanakuntdbu heat Qd chumla ike
pexnmaniuty
aleshal Teb waking it masive
Stneh
h i0diur sollutiom yllew) u added o
l oblu blatk lode
SBluion ushitt , Hhe later huns
Stagan, huuee remainsele
des uet taet wuith simplz
He
bluw blask Solutien,
s a l i v a added o
saliva breaks douwn
uzym Salivanu auyiase palazut
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pleneerpoporco.com
T. NO.
PACENO. 57
Staneh aud 4he Celour e solution ethangs
bluu black to u ddia Oueun auud HaLw 1oiH
DLigima
yCle eelour
. he cowmplei buLakdown o Staeh mto Stmple suuqar
Ca be tested by addinq beuedict's selutie (blut) to
e Teachion mixture amd heatuA he slution.Th
Colour e w Seuutiew will ehange iem btuu ruw
yeuow 7Tange 1ndicatug the rtsL S
Simmptr Sugans ud absee starch u Hu selution
PRoCEDURE Seuits teat hubs hawimg Iml oiodine Selutien
eaeh u prepared. wo sets olcdiue ubes e made aud
y a kupt u S2paaali Stauds
2. Ln a test tuube, bml o oe Staedv solution aund
Nal b added. l t labellld as expvimen-
I l 1
klbe, 5ml 1/ Stareh
al ube In aun0tn tust
tabelled
1Nac i added. 1
souiow and, wl
put m a
h t tut hubes are
as Cotol uube, Now
or
mantaiuntd at $FC appuoxiwakuly
wattr batt teuperature is
Somat he Came
about 10minutu
tem.CIn suniwer a beaker fiulud wit
aloo asauired by
Pwrpose.
uaed or uis
unter eau be is_added
ud ml owaler
CR dilute uzym chvely
3. me
aud e kebe Tuspe
coutrsl kebe
he eouhsl
rispechvely
e p v i m u n t a l

hube auud
o u taeh SL Hu
ea dzepper, a drrp t m
WiA ue help
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FACE0
EXPT. NO.
Ha tubes is Aakeu aund owud ino wo sa ponate ubes,
CGtcunm iDcdine h o hwo tois iadine ubu-
o eimuntal aud oher dor oontol) w liu e
Add.mg oted a zetto minuk TRadin
4 Alter n ntvwal o 2 wimuutes, a dep agoin
Hakew Dm ea eapvumttab and comtrol kubes ad
pOuMd e iodune tubes, T change telour e
iodine i noted.
5. Te pLoCs u kept on repLaing_atu aw intewal 6
NUy 2 minutu Hu nw ohauge iu Colour odineL
8etn lo dime for cortel ube always ives phuu
|9lour noted
PREPARATONS ; Stareh solutiow To 10 mls disid water
add 4e Ssbe Stare to ake 'tt iuto a thick
paate. e l 40 l edishilid water aud to it add löm]
o wstrs paste alread prepaxed gradually by
Ha owe He coluiew bveunight aud h ptter
o get lee Storeh salution
2, 1 Disscwe lq NaCl u lo0 ml o
Nae, selutien
ishilud mawn
ilm sl eot on
3. Celletusw et Saliva Maki a hw
ue water ol e
Laud dip_i ulo wabn, Drain
unntln
Cotto aud sp1ead it_over 4he wouih
ilter. Ketp4ue funne
SLeh away a t it aet as
IE
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PAGE1O. 5
NO.
OVex_a clean teat ube Now ekuv a puw o
ubber
And pou h ius owimg caliva 'wuto he funnt.
Fizteved
caliva wil eouuet iu he teat tube, Take m of
Saliva_aud add 1ml water to aet 1:20 diiudio
Hough
PRECAUTIONS1FLLter e saliv ueugh aAt Cottom ilm y not
aittor Paper.
Should bedone ver4
2.AU e weighiaad munauiug
aeunatel
teunporatume he water for both,
. Mauntaun unirm
ouout w £ptrmunt
SaliYa, Mre Should be
no avr _bubbles
4.whilu miasuug
4

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 piefeerpaparco.com
FAGE NO.
ExPERIMENT I4o
AIM
loStudy he ediuet e dißtaut eeprakurs on hu
attivity 0 Caliva anylase on stauh
APPARATDx
RE SUIRE0 i s t tubes, ttat ehube stauds pipetis , biakeu ectton,
wnt, water balh, Spiit lap o buner , main
Cindr Stanew Soduw ahlornde dis tilled water , odine
Selution iodide, ete.
PRDCEDVRE hru Smu_eL ttat kubes hawim _04 iodine selukion u
ean made Tmy are kept sepoatrly u rec stauds_
2. Tr expeuimeuta ttat hubeu iuh havin 5ml o1
SHarew and ml 1:aev
selution u wmade
Tuy ae maintaámed at hr dikfernt tumprature viz.
6t2'c, 3712C and *0 T 2 dilrt waer bats.
Tomauntou low tuaperakue ef 5°e make se o iec)
aul he ure
3. m o_diluted euzyme added
edopPper, a dep u
exunimunta tubes. wHh he help
tub_amd added o tube con tainin
takn dm heae Btat
addimq oked as Zep muinutt
iOdineTu Hu
umute again a doop
a take
Altr an mterwa, o
tubes aud
poutd it touiodiu
Jots eaeh ube aud xPuimeul is
noted. k
CoLowr iodin
he chauge
K
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 eoneerpeperc com
FAGE NO.
kep 0n TLqLakimq at aw imtLua
bi Wtm 2 minutis4
u e alour 0dime dats unt thoug
5. Te
ue metaken
ox by dillrunt expuimuntou, ubes
noted hu my do no qive ony 0elour with iodi
PREPARATONS1. - stael Seluton lo 10 ml o dis
distild tilud water
water add
add
19 Suub Staruh to ak it uto a hiek
posti. Bol
40 ml i datlled nake aud to it add 10 m el the staeh
poate alrrady perepred, aTaduall by sthiAung Leawe u
Suton_ovwtnght and n Rttts to at 1 Stae
geutiom
. Nacl solution Disole lq_Nael in 100 m 6 distAlled
atr.
. CoRkion oL Saliva ' Make a hn tm
9} cotten
dip t 'to water. Drain o A_waler ofhe coton
SPTOad it vr he nLoutw t funne Sueb a way hat ut
EMPERATURE TME
M AENMUTE ) To FE AC THE ACtIRDnc PoiT
PoinT
52
50
 poneerpopercs.com
FACE No. 2
PRECAUTONS Fittr thu saliva urough a wtteotton ilm Qtad not
hong 0ilter paper
2. AU_ he wrigmiug aund owiug suould be don vey_
areratelu
. Maiwtaur uukonm temprature watuT for looh
hotugout ue expniment
4. whide asuning 9iva, hNe should be wo ain
yublblus.
5. Au Melaswanes wed wwt be Hhovougy eleausd
aud died.
 Tk owutir

A C

aker
37C

PL
PH 6-3

l Na
5 m stovtch b

PH
water

E Lhut ot pH aeliny ayoet,

*
 E'oneorDoorco.com

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FACE O . 3

ExPERIMENF14o

AIM To study hu e4et e diltrut qh on oulivity of

Salivan Quytane on starnew.

APPARA TUS
RE QUIRED a t tubus, kest tuube stande,beaker, pipettes, knnts
wtAoivq eyimdur, 0stoy, Statew ioduw, p0tassium iodide,
Sodium ohloide, diternt pH jabluts

YRDCEDURE Twr Sriu o ttat tubu hauing lml o iodine selutieu

eat madu hy ne kipt Separntely n Haru
Staunds.
. To a tuat ube 5ml 9t l; starue. selutien , lml o 1p
Nae d a tabut e ku0w OHSay PH-5) i addkd,
to auetwe teat wbe bmk 0 Stauh seution , Jm
1 Nae and_atablut o Rnouu ph (say pH 6-8) =

added and Ao me huud teat tube 5ml ef l. stare

kuoou pH tablut
SeLuievw Nae aud o
(Say PH added.
3. Twa Best Jubis ae put a a watur baBw at 37C,
ox 10 wimuts A beaker iud with ater ean be uud
foT w mpoduuing Surwmr.
4.o tmae trat ubes, lm s iluted euzymu addid.
Iwmediately wi help o sepamate dooppLTS a
DATE
-ploneorpeperc
FAGE NO. 4
T. NO.

dhep How eaelh lzst lube is takew taken aud poumed

e 1 t a t ubshoaim iodime souion. In i s
Way Mvu Semes odime
odime. tubes- 0nelor pH= 5, auotur
for pH= 6:8, aud e mird o eorpH= 8 pripazed
T Hie oaddima woted a zem minute. Twse
At ubs ane kpt w waBer bat u0ughout he
e7uiwt.
. Aptur aw imBvwoal o minute, a dep tm eaeh
4uube t again takew aud poured it 4o he 10dine
aud _chauge Colour iedme u Moted
S 1kis ezpuimuut i kept ow repeating ajtr a
imtewa O evey2 mimuts tiu celour iodime
doLs uot cuange hotal t_takew for uase tubes
s noted Hu uy do nol ive eelour wi iodine

Sounon

PREPARATIONE:1 1 taalsolutioni To 10 ml o dishlud water add

l souble Starek o waite it into a hick paste.
aiu ao n y disillud water and o it add 1 ml o e
staeh paat alvuady prepard 2 qradually hy stiina
Leawe e gsluthewovnigitauAd n itter to get
taeh soutionw
OBSERVA TIONNS

REACTION WITH LO DINE

TiME
(IN MNUTES) TEST Tuet ATH TEST TuBE wITH TEST TEF IT
PH
B - bladk colowr
tu-elack celour P e celour

BluL e l e u r BuL- Blok ete

Cu-blotk colour
b l a s k ielo wr
A ur-eloek celout olut esloun

uu- toek olour Bu 2olewr eu a ek celgtm

Gl otatk celour No ohae n slour Bluu blatk aetewr

iod
bloak ecler
Ne_one m celav
10 Cl-blok tslour
iod
DATE

e.oneerpeperco.com-
FACE NO.
PT. NO.

CuALd dip it utb water. Tain el

water cotton
d sphLad it oVI H oul 8 lunu iu uuk a
way
mat 'it_al a ter. Ketp e unn over a eliaw
eat tube. No chw a_ puuu 0f mubber ad
pour w
1weomig Saliva 'to Junnl. Filturd saliva will
eot n he teat tube, TakL Imb e taliva aud
dd 1ml e aalir toaet 1:20 dilutiow e he euzym.

PRECAVT1ONS 1Lter me Saliva ngh a u t eotion ilm aud net

Mrpuah terpopes
2 A Ha weLignimg aud wnoimg gheuld De dou voy
arrwratty.
 pioieorpeperco.co
PAGENO. 6e

. Maitaun niform teuupwoturee 0 Me water fo bo2

bo,
wwpuahout e epniwunt.
4. wilw maauwiu4 Saliva, hareChoruld be no air
bubblus
5. Alhe glasEuwares satd wuwst be. horeughy cleatd
nd dMed.