tnpaimtuf - T

Estimafiow o Tactein
1TH Auqust,2021
*Dbjecfive Estimadiev of psubtein usin
Folin o wrys
Folin - l o w r y

Hekhod

TAintiple: the two Aeactious ueki e twplaiu H

painei ple
PADdein- ut
(i) Cu 2+
+ PoDteiu
pepHde
akaliue Coep \en

Cone e du ud
bono
PPs)
ii) poote lu + Folin Paotei
Cbe plen Cio caltae u Lopen
ple
tbmplen)
h hwo lit abu|
ab he amino aci el tyaosiue
CLn o uill PAodte a bue
t p to an) hesiou

puple toLDWk phoipeo wLD ly b do tungstate

to tend- po uo Ry b eltwuum be
bue u topp
d aciels
cotaysed iAttouo e ani uo

T
The epuple tblouk is osme o baic

acol t ptopau OLnol tyAdsine

1ued to ind H tonuntrat-
P amb ut's aw
i o e as uelaiomshi p eietr
-ion
o Subs tau e
f s Fand aad touti ens
in meas wr sef

touaiuing e ame &ubstan e

olowin Pelaiou 1
us ed to ne (onentration
A=ELC "3 g() toT
A -Absoub ane
E Molax absoptie ty
pah Reng H

C Lonuntaation oSouumple
T. ini tial utensi
T tinal tntnsih
T- tAans mitlan u
*Appanaus
iu o.tM
Alkalie Na, co, solution: (20 qe Na, to,
Na,C03 O H)
tusO
CuSO Sodium potassium taataaate soluton
iu sodiu potassiu m
S a / CusO4sH,o gtock
tastasate). Pacp ass aesh by
SoLutious ay o
use

(iit) AAkaliu 3o Lutioun Phepase en

m
So'lution ahd
sotio n 1 Hhe
(Diute tO meactal
Foiu -

Cio calt eau Aeagent

we u m e waat o
htaq ent wi eu
o So olfuu
a o
mo
ly b d afe i
tun q state an e So ot u m

a n el
elro edAoRTO acfel
phorp ho si
e

Ab u i a ock
(w) Stand and poteiu Bou uc auu
solutt e mewml)
 FeftLod
Add 2.5
wi of alkaliue SoRution to o.elo 4 test
o stand at
at Solu tion
andalouo
Hin
iosDuaL
oabugQLy
tewp e o t u io minuttes
R Long4 es
io calttau nea qent
A a 0.25 m oli ute oliu -

wi iwmeoli afe
hapid
Yhe a q ainsF
minut es Maol Hhe oubso Abanu
4tt 30
blank at
aPPAUpaiafe
an unknbuau
Estimate Hu psofeiu toutunt aatiou
tand an wne and Stanh
So uuki ow ata ppaniugas al
is to be papaktd uslu difaent cbntentratieus

O kKuo uon Sel Hon (b.I -

oU.Sw
bbsevatton Table

Tube hotein Ne kume ot Veume ot VoRume et nubate Vel. Inaubot .

Contentrafiou Paoteim elistilee alkalie at at
No. FC RT inD0
in in wl) wata Soluion RT
Cin ml) ml) in wi), (iu a
win 45D wm)

Blank b.5 2.5 .2S 3 O

.1 0.0S .S 2S lo 0.25 30 o.16

2S 10 0.25 60 O28
2 0.2 0.I

3 3S 2.S lo 0-25 30 319

0.3 0.1S

4 0-2 .3 2-S 10 0.25 30o t.48T

0-25 2. 10 . 2 30 6.SS4
0.S 6.25

Ht unk 0.S 2-5 lo 0.25 30 0.312

Result The mo unf o

f potei pesenf 1u e
tu
S n p le

as foun e tobe 0-30 6g n