Seminario Espectroscopía de electrones Inmaculada Ri Plaza Gómez

Fluorescence imaging of biological tissues is important for clinical applications. There

are hundreds of complex organic components existing in biological tissues, leading to
major optical absorption at specific wavelength region. Therefore, in practical
Fluorescence imaging of the biological tissues, materials that process ultraviolet- visible-
near-infrared (UV-Vis-NIR) wide optical emission can be favorable.

Due to the simple chemistry, facile fabrication, and exceptional performance the Ag-Cu,
metal organic compound nanoparticles are envisioned to play a significant role in many
low-cost and rapidly processed bio-tissue UV-Vis-NIR imaging applications.

In the figure, Ag-Cu compound spectra of C1s is shown:

c
b

a) Determine the C1s binding picks and give an explanation.

b) The C1s binding energies of the spectra are: 284.4 eV, 285.7 eV and 288.3 eV.
b.1) Relate the value of the energies considering section a).
b.2) Taking in consideration that the energy of the incident x-rays is 600eV.
Calculate the kinetic energy of electrons and speed of the electrons.
Seminario Espectroscopía de electrones Inmaculada Ri Plaza Gómez

Solution:

a) Determine the C1s binding picks and give an explanation.

The binding energies depend on the specific enviroment where the functional groups are
located. In the spectrum the binding energy increases to the right.

• C-C bond will have the lower value of binding energy considering that is a simple
bond and there are no electronegative elements.
• O-C=O would have the highest value of binding energy. There is a double bond,
ᴨ bonds electrons are more easily polarized and also there is O which is a
electronegative element that removes electrons causing the binding energy to
increase.

b) The C1s binding energies of the spectra are: 284.4 eV, 285.7 eV and 288.3 eV.
b.1) Relate the value of the energies considering section a).

Functional Group Binding energy (eV)

C-C 284,4
C-O-C 285,7
O-C=O 288,3
Seminario Espectroscopía de electrones Inmaculada Ri Plaza Gómez

b.2) Taking in consideration that the energy of the incident x-rays is 600eV.
Calculate the kinetic energy of electrons and speed of the emitted electrons.

Ekinetic = hν − Eb

Ekinetic = kinetic energy of the emitted electrons.

hν = energy of the incident X-rays

Eb = binding energy,

Functional Group Eb (eV) Ekinetic(eV)

C-C 284,4 315,6
C-O-C 285,7 314,3
O-C=O 288,3 311,7

Ekinetic = hν − Eb = 600eV − 284,4eV = 315,6 eV

To calculate the speed of the emitted electrons we proceed knowing that the kinetic
energy is:

1 2 × Ekinetic
Ekinetic = × m × v2 → V = √
2 m

m: electron mass = 9,11 x 10-31 kg

1 eV = 1,6 × 10-19 J

Ekinetic(eV) Ekinetic(J) V (m/s)

315,6 5,0496 × 10-17 1,0529 × 107
314,3 5,0288 × 10-17 1,0507 × 107
311,7 4,9872 × 10-17 1,0464 × 107

2 × Ekinetic 2 × 5,0496 × 10−17

V=√ =√ = 1,0529 × 107 𝑚/𝑠
m 9,11 × 10−31