FACULTY OF EARTH SCIENCE

EFT 1263 CHEMISTRY IN APPLIED SCIENCE

SEPTEMBER 2021/2022

LAB REPORT:
[STOICHIOMETRY : ACID DILUTION PROBLEM & GLUCOSE DILUTION PROBLEM]

Prepared by,
No. Name Matric No.
1) Hannah Binti Mohd Fisal E21A0482
2) Siti Nur Izzati Asyikin Binti Mohamed Asri E21A0673
3) Nur Syazarina Binti Mohd Sayuti E21A0627
4) Amer Zulkarnain Bin Azman E21A0452
EFT1263 Lab report Chemistry in Applied Science
Bachelor in Applied Sc. (Hons.) Natural Resources Sc. Faculty of Earth Science

STOICHIOMETRY : ACID DILUTION PROBLEM & GLUCOSE

DILUTION PROBLEM

Hannah Binti Mohd Fisal

Faculty of Geoscience, Universiti Malaysia Kelantan, Jeli, Malaysia

E-mail: e21a0482@siswa.umk.edu.my

Abstract
Dilution occurs when a certain volume of solvent is added into a solution with the purpose of changing the concentration
of the solution. Despite adding more solvent, the amount of solute stays the same. By using the correct formula we are able to
find the amount of solvent required to change the solute to a desirable concentration. Students were given two scenarios; the
first scenario requiring students to produce 500ml of 3M HCl from 2.5L of 11.6M HCl. The second one required students to
create 1L of 0.025M of glucose solution from 10ml of 1M glucose solution. The formula C1V1 = C2V2 was used to calculate
the volume of stock solution and the volume of distilled water required. The concentration of stock solution is placed in the
place of C1 and the volume of stock solution is placed in V 1 while the concentration of desired solution is placed in C 2 while
V2 will be denoted with ‘x’. Then the total volume of desired product is subtracted with ‘x’ to find the volume of distilled
water required. By slowly adding the stock solution into the distilled water, the final products 500ml of 3M HCl and 1L of
0.025M glucose solution was formed. After every experimental procedure, the apparatus used were cleaned and carefully
stored in the proper locations.

Keywords: dilution, concentration, solution

1. Introduction apparatus; a measuring cylinder, beaker, pipette and

Solute refers to the substance that is mixed within a
solvent. A solvent on the other hand is a substance that
dissolves a solute. A solvent is usually liquid but it can also
be a gas.
Concentration is the ratio of solute with the solvent it is
mixed in. When a solution can no longer dissolve a solute
then the solution is said to be saturated. By adding more
solute, we can change the concentration of solution. There
are many ways to denote concentration. Molarity is one of
the most common ways to denote concentration. It denotes
the number of moles of a given substance per litre of
solution.
Dilution is the addition of solvent which decreases the
concentration of the solute in the solution. In both dilution
and concentration, the amount of solute stays the same. This
gives us a way to calculate what the new solution volume
must be for the desired concentration of solute.
volumetric flask of different capacities were used.
There were two separate scenarios given in this lab session.
The first required students to produce 500ml of 3M HCl from
the given 2.5L, 11.6M HCl. In the second scenario students
were tasked to produce 1000ml or 1L of 0.025M glucose
solution from the stock solution 10ml of 1M glucose
solution.
For both scenarios the equation C1V1 = C2V2 was used to find
the volume of stock solution required to form the desired
product. The concentration of stock solution is placed in the
place of C1 and the volume of stock solution is placed in V1
while the concentration of desired solution is placed in C2
while V2 will be denoted with ‘x’. The volume of stock
solution required was then subtracted from the total volume
of the final product to obtain the volume of distilled water
required.
1.1 Procedures for Acid Dilution

2. Materials and method The volume of HCl required to produce 500ml of 3M HCl
was calculated using the formula C1V1 = C2V2. 370ml of
Materials used for this experiment are 2.5L of 11.6M HCl, distilled water was poured into a volumetric flask while
25ml of 1M glucose solution and distilled water. As for the 130ml of 11.6M HCl was poured into a beaker. A pipette

1 © 2019 Faculty of Earth Science, UMK

EFT1263 Lab report Chemistry in Applied Science
Bachelor in Applied Sc. (Hons.) Natural Resources Sc.
was then used to slowly drip the 130ml 11.6M HCl into the
volumetric flask containing distilled water. The final product
was 500ml of 3M HCl.
1.2 Procedures for Glucose Dilution
The same formula that was used in Scenario 1, was used to
find the volume of distilled water and 1M glucose solution to
produce 1L of 0.025M. Another volumetric flask was filled
with 975ml of distilled water while another beaker is filled
with 25ml of 1M glucose solution. The third step in the first
scenario was repeated with the new stock solution and 1L of
0.025M glucose solution was produced.
3. Results
By using the formula C1V1 = C2V2, it was found that for
the first scenario, the volume of HCl required was 130ml and
the volume of distilled water required was 370ml. As for the
second scenario, it was found that the volume of distilled
water required was 975ml while the volume of 1M glucose
solution was 25ml. Since the volume of stock solution was
insufficient another 15ml was added to the initial 10ml of
stock solution. By slowly dripping the stock solutions into
distilled water we obtained 500ml of 3M HCl from the first
scenario and 1L of 0.025M Glucose solution from the second
scenario.
4. Discussions
The purpose of diluting a solution is to change the
molarity or concentration of a certain solution. The concept
of dilution is a very important industrial application. In
Geology, one example of application of the concept of
dilution is in ore dilution. Dilution refers to the waste
material that is mined with ore and is not separated from the
ore during the operation. This waste is then sent to the
processing plant. Dilution increases the operating costs by
increasing the tonnage of material to be milled. In addition to
its direct impact on the income of a mine in the short term,
dilution will cause significant changes in other factors that
will reduce the overall value of the project in the long run.
Hence, it is important to properly understand the concept of
dilution to be able to properly apply it to real life geology.
The equation used in this lab is C1V1 = C2V2The
difference between the two formulas C1V1 = C2V2 and M1V1
= M2V2 is that M represents molarity specifically while C can
mean concentration in any form. C1V1 = C2V2 is a formula
with wider use and in applied science it can be used in almost
any dilution technique. The concentration of a solution is
inversely proportional to the volume of a solution. This is
because, concentration is the measure of amount of solute
2 © 2019 Faculty of Earth Science, UMK
Faculty of Earth Science
divided by the amount of solution, hence when the volume of
solution increases, the concentration must decrease. When
diluting corrosive substances such as acid, gloves must be
worn to avoid contact with skin. After every experimental
procedure, we must always clean the apparatus used
carefully and store them in the proper locations.
Acknowledgements
I would like to thank Dr. Nor Shahida Shafiee for the
guidance she gave my lab mates and I that helped in
completing this lab experiment and report. I would also like
to thank my lab mates, Siti Nur Izzati Asyikin Binti
Mohamed Asri, Nur Syazarina Binti Mohd Sayuti and Amer
Zulkarnain Bin Azman.
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