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MEASURE RATE OF DISAPPEARANCE OF SUBSTRATE

calculateslope oftangent
attimeO

É
FACTORS AFFECTING onlydenaturewhen
temptoohigh
Temperature IRREVERSIBLE

rate
Fastest assymetrical
IIYPzggigggjsgg.gg
fading vibrate much
bond as too vibrationstronger Tek
and
break
t ed M Et in substrate hydrogenbondsholding

ÉÉÉI É
I
movefaster shapeofenzymebreak

substrate enzyme
sub enter As moreoften looseshape not
me movefaster
frequentcollisions
Esc formmore frequently complementary tosub
mannnn
esctrea rateareaction my e um
IRREVERSIBLE
soreactionoccur irreversible

OPTIMUM TEMPERATURE

submustbindAS
sub enoughenergyto
overcome
Ea successfulcollisions

Disulphidebondnotsusceptible to hightemperature

INACTIVE AT LOWTEMP
enzyme t substrate Ekh
moveslower collideslower
Esc formed N freq
fewermot haveEa
formationofproduct disappearance of reactants
Obtain VolofOz
measure rot ofOz in 5min
temp INDEPENDENTVARIABLEconange
at least 5 diff values of
Ig

É it temps to to
rate mint
Of em

Breakdownprotein

preventthemfrombeing denatured

Workbestathightemp

Inc reaction rate

PH
conc of Htions interact with estaticattraction of Rgroupof Aminoadd
affectelectrostatic attraction Hydrogen ionicbond
bond holdingshapeofactive sitechange affect3Darrangement of enzymemolecule
activesitenolongercomplementarytosubstrate cannot bind
denature
buffersolution maintainpH resistchange ofpH

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