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  • Calibration Curve, Spectrophotometric Method

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    Nidhi Hegde
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    This document contains data from a calibration curve experiment measuring the absorbance of solutions with increasing concentrations of phosphate. It lists the concentration of phosphate stock solution, volume of working solution used, concentration of phosphate in the colored solution, and measured absorbance. A graph is shown of the linear relationship between absorbance and concentration of phosphate, with an equation for the best-fit line.

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    Calibration curve, Spectrophotometric Method

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    This document contains data from a calibration curve experiment measuring the absorbance of solutions with increasing concentrations of phosphate. It lists the concentration of phosphate stock solution, volume of working solution used, concentration of phosphate in the colored solution, and measured absorbance. A graph is shown of the linear relationship between absorbance and concentration of phosphate, with an equation for the best-fit line.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    7 views1 page

    Calibration Curve, Spectrophotometric Method

    Uploaded by

    Nidhi Hegde
    This document contains data from a calibration curve experiment measuring the absorbance of solutions with increasing concentrations of phosphate. It lists the concentration of phosphate stock solution, volume of working solution used, concentration of phosphate in the colored solution, and measured absorbance. A graph is shown of the linear relationship between absorbance and concentration of phosphate, with an equation for the best-fit line.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 1

    Concentration Volume

    of of Concentration
    phosphate working of
    stock solution, solution P in colored
    M used, mL solution, M Absorbance
    5.00E-03 1 6.00E-06 0.154
    5.00E-03 2 1.20E-05 0.312
    5.00E-03 3 1.80E-05 0.482
    5.00E-03 4 2.40E-05 0.654
    5.00E-03 5 3.00E-05 0.792

    Absorbance VS Concentration P
    0.9
    0.8 y = 26967x - 0.0066
    0.7
    0.6
    Absorbance

    0.5
    0.4
    0.3
    0.2
    0.1
    0
    0.00E+00 5.00E-06 1.00E-05 1.50E-05 2.00E-05 2.50E-05 3.00E-05 3.50E-05
    Concentration P

    Calibration Curve By- Katlyn Andrerson, Shreenidhi Gireesh


    Hegde, Chariya Chhay, Lauren Duerr

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