Properties of Fluorescent Dyes

Fluorescent Dye Structure MW λabs λem εmax Isomers Notes/

Advantages
Color (free acid) [nm] [nm] [L*mmol
[g/mol] -1
*cm-1]

Spectrum similar to
Near-IR DY-776 813 771 793 120 pure LICOR IRD 800RS.

Near-IR DY-751 793 751 779 270 pure Spectrum similar to Cy7.

Spectrum similar to
Near-IR Cy7 683 756 779 250 pure Alexa Fluor 750.

High thermal and

Near-IR ATTO 740 undisclosed 467 743 763 120 pure photostability, but pH
sensitive.

Red ATTO 700 565 700 716 120 pure Substitute for Cy5.5.

Substitute for Atto 700,

Red Cy5.5 917 684 710 198 pure Alexa Fluor®680 and
DY-680.

Substitute for Cy5.5;

spectroscopic
Red ATTO 680 525 681 698 125 pure properties similar to
LICOR IRD 700.

Suitable for STED

Red ATTO 665 undisclosed 622 662 680 160 pure applications.

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Phone +49-3641-6285 000 | Fax +49-3641-6285 100
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Properties of Fluorescent Dyes

Fluorescent Dye Structure MW λabs λem εmax Isomers Notes/

Advantages
Color (free acid) [nm] [nm] [L*mmol
[g/mol] -1
*cm-1]

Substitute for Cy5, but

Red ATTO 655 527 663 680 125 two better since no triplet
behavior.

Spectrum similar to Cy5

Red DY-647P1 687 653 672 250 pure and Alexa Fluor®647.

Substitute for Alexa

Red Cy5 657 649 670 250 pure Fluor®647, Atto 655 and
Atto 647N.

AF647
Red (also known as 873 650 665 270 pure Substitute for Cy5.
Alexa Fluor®647)

Substitute for Alexa

Red ATTO 647N 646 646 664 150 two Fluor®647 and Cy5; base
stable.

Hydrophilic substitute
Red ATTO 643 undisclosed 836 643 665 150 three for ATTO 647N.

Emission maximum of
Red ATTO 633 552 630 651 130 pure He-Ne laser, base
stable, less hydrophilic.

Red ATTO Rho14 784 626 646 140 pure

Jena Bioscience GmbH | Loebstedter Str. 71 | 07749 Jena, Germany

Phone +49-3641-6285 000 | Fax +49-3641-6285 100
www.jenabioscience.com Last update: Nov 04, 2020
Properties of Fluorescent Dyes

Fluorescent Dye Structure MW λabs λem εmax Isomers Notes/

Advantages
Color (free acid) [nm] [nm] [L*mmol
[g/mol] -1
*cm-1]

Substitute for
Red ATTO 620 512 620 642 120 pure LightCycler Red 640;
base stable.

Large Stokes Shift of

Red DY-480XL 515 500 630 50 pure 130 nm.

Substitute for Alexa

Fluor®594, more
Red ATTO 594 805 603 626 120 pure hydrophilic than ATTO
590.

Orange ATTO Rho13 647 603 627 120 pure

Substitute for Texas

Orange ATTO 590 590 593 622 120 two Red, Alexa Fluor®594.

AF594
Orange (also known as 723 590 617 92 two
Alexa Fluor®594)

Orange ATTO Rho101 591 587 609 120 pure

Jena Bioscience GmbH | Loebstedter Str. 71 | 07749 Jena, Germany

Phone +49-3641-6285 000 | Fax +49-3641-6285 100
www.jenabioscience.com Last update: Nov 04, 2020
Properties of Fluorescent Dyes

Fluorescent Dye Structure MW λabs λem εmax Isomers Notes/

Advantages
Color (free acid) [nm] [nm] [L*mmol
[g/mol] -1
*cm-1]

Orange Texas Red 720 588 609 80 two

Orange ATTO Thio12 502 582 607 110 pure

Orange ATTO Rho12 650 576 601 120 three

Orange 6-ROX 535 575 600 82 pure

Orange ATTO Rho11 566 572 595 120 pure

Substitute for Cy3, Alexa

Fluor®546, Bodipy
Orange ATTO 565 511 564 590 120 two 558/568; better
photostability.

AF555 (structural analog

to Alexa Fluor®555) is a
hydrophilic dye with
Orange AF555 861 555 572 155 pure excellent photostability
compared to
fluorescein.

Jena Bioscience GmbH | Loebstedter Str. 71 | 07749 Jena, Germany

Phone +49-3641-6285 000 | Fax +49-3641-6285 100
www.jenabioscience.com Last update: Nov 04, 2020
Properties of Fluorescent Dyes

Fluorescent Dye Structure MW λabs λem εmax Isomers Notes/

Advantages
Color (free acid) [nm] [nm] [L*mmol
[g/mol] -1
*cm-1]

AF546
Yellow (also known as 848 556 573 112 pure Substitute for Cy3.
Alexa Fluor®546)

three Substitute for Cy3, Alexa

Fluor®546, Bodipy 558/
Yellow ATTO 550 595 554 576 120
60%:22 568; better
photostability.
%:17%

Yellow 5/6-TAMRA 430 545 575 90 two

Strong absorption peak

at 550 nm and a
secondary absorption
Yellow Cy3 631 550 570 150 pure peak at 520nm;
Substitute for Alexa
Fluor®555, DY-550 and
TAMRA.

Yellow-
ATTO Rho6G 515 533 557 115 pure
Green

Yellow-
5-SIMA 597 533 557 64 pure
Green

Yellow- Large Stokes Shift of

DY-485XL 503 485 560 50 pure
Green 75 nm.

Jena Bioscience GmbH | Loebstedter Str. 71 | 07749 Jena, Germany

Phone +49-3641-6285 000 | Fax +49-3641-6285 100
www.jenabioscience.com Last update: Nov 04, 2020
Properties of Fluorescent Dyes

Fluorescent Dye Structure MW λabs λem εmax Isomers Notes/

Advantages
Color (free acid) [nm] [nm] [L*mmol
[g/mol] -1
*cm-1]

Substitute for Alexa

Yellow-
ATTO 532 646 532 552 115 pure Fluor®532; Bodipy
Green 530/550.

Used as substrates for

Yellow- TNP G-proteins and ATP
(also known as 229 470 552 19 pure binding proteins. Also
Green Picric acid) available as pyrimidine
probes.

Yellow-
6-JOE 505 520 548 75 pure Substitute for VIC, HEX.
Green

Green ATTO 495 352 498 526 80 pure

Green 5/6-RHOX 374 501 525 84 two

Substitute for FITC,

Alexa Fluor®488, Cy2;
Green ATTO 488 590 500 520 90 pure identical to Alexa, much
more photostable than
Cy2 or FITC.

AF488
Green (also known as 534 495 519 73 two
Alexa Fluor®488)

Jena Bioscience GmbH | Loebstedter Str. 71 | 07749 Jena, Germany

Phone +49-3641-6285 000 | Fax +49-3641-6285 100
www.jenabioscience.com Last update: Nov 04, 2020
Properties of Fluorescent Dyes

Fluorescent Dye Structure MW λabs λem εmax Isomers Notes/

Advantages
Color (free acid) [nm] [nm] [L*mmol
[g/mol] -1
*cm-1]

Green 5/6-FAM 376 492 517 83 two

BDP-FL Substitute for FITC,

Green (also known as 292 503 512 80 pure Alexa Fluor®488.
BODIPY®FL)

Blue-Green ATTO 465 296 453 506 75 pure Substitute for Cyan 500.

Blue-Green ATTO 425 401 439 485 45 pure

Blue ATTO 390 343 390 476 24 pure Large Stokes Shift.

DEAC
Blue 261 409 473 56 pure
(free acid)

AmNS
Blue (also known as 223 336 455 5,9 pure Large Stokes Shift.
Laurent acid)

Jena Bioscience GmbH | Loebstedter Str. 71 | 07749 Jena, Germany

Phone +49-3641-6285 000 | Fax +49-3641-6285 100
www.jenabioscience.com Last update: Nov 04, 2020
Properties of Fluorescent Dyes

Fluorescent Dye Structure MW λabs λem εmax Isomers Notes/

Advantages
Color (free acid) [nm] [nm] [L*mmol
[g/mol] -1
*cm-1]
Small MW and
environmentally
sensitive fluorescence.
Blue MANT 151 355 448 5,8 pure FRET-probe for protein-
intrinsic Tyr and Trp
residues.

Large Stokes Shift and

AMCA high photostability,
Blue 233 346 434 19 pure commonly used for
(free acid)
multi labelling.

Blue ANT 137 332 423 4,6 pure Substitute for MANT.

Jena Bioscience GmbH | Loebstedter Str. 71 | 07749 Jena, Germany

Phone +49-3641-6285 000 | Fax +49-3641-6285 100
www.jenabioscience.com Last update: Nov 04, 2020