Name Maisha Tasneem Student ID: 20920373 Section: LA1

Department of Chemistry
The Hong Kong University of Science and Technology

CHEM 1050 Laboratory for General Chemistry I 2022−23 Fall

Lab Report 1: Chemiluminescence (To be submitted in Week 5)
[Total: 10 points]

PART I. Experimental Results

1. Observations (effervescence and color change) after addition of 3% H2O2 solution to 0.25%
(w/v) luminol in 1 M NaOH solution. [1
point]
effervescence: no effervescence

color change: no color change; the solution was clear

2. Observations (effervescence and color change) after addition of 50 g/L K 3Fe(CN)6 solution to
the aqueous solution containing luminol, H2O2 and NaOH. [1
point]
effervescence: Gas bubbles were seen, so the reaction produced effervescence

color change: the color of the solution turned blue for a while after the addition of
K3Fe(CN)6 before it became clear again

3. The difference in observations between additions of 50 g/L, 25 g/L and 12.5 g/L K 3Fe(CN)6
solution to the aqueous solution containing luminol, H2O2 and NaOH. [2
points]
the factor varied in this experiment: the concentration of the K3Fe(CN)6 solution varied
 the difference in observations: The duration of the light emission and color change was
different for the three K3Fe(CN)6 solutions with different concentrations– the more diluted
the solution was, the shorter was the duration of light emission and color change

PART II. Questions

1. What are the roles and functions of H2O2 and K3Fe(CN)6 in the chemiluminescent reaction
performed in this experiment? [2
points]
Role Function
Oxidizes luminol to produce acid, nitrogen gas,
H2O2 Oxidizing Agent
water, and light

K3Fe(CN)6 Catalyst Triggers the reaction

2. Consider the preparation of 50 mL of a 250 g/L K3Fe(CN)6 solution.

a) Calculate the mass of K3Fe(CN)6 powder required for the preparation. [1
point]

Concentration of the solution (g/L) * volume of

mass of K3Fe(CN)6 powder required =
the solution (L)

= 250g/L * 0.5L

= 12.5 g

b) If the volume of K3Fe(CN)6 powder does contribute significantly to the final volume of
the solution, describe the procedure for accurately preparing the solution after weighing
out the required amount of powder. [3
points]
 1. We take the desired amount of powder in a 50mL volumetric flask.
2. We fill the volumetric flask with DI water until the lower meniscus reach around
the 30mL mark (or two-third of the flask).
3. We then stir the solution using a glass rod until the powder is dissolved and we
obtain a yellow solution.
4. Lastly, we keep adding DI water to the flask until the solution’s lower meniscus
reaches the 50mL mark. We can use a dropper to pour the DI water for precision.
Thus, we will obtain the solution with the desired volume of 50mL.

***Remember to insert the image of the signed Data Sheet to Appendix on the last page.***
PART III. Appendix
Please scan/photograph the signed Data Sheet as .jpeg file and insert the file below.
(make sure your personal details, data and Staff’s/TA’s signature are clearly displayed)
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