Scribd Logo
Upload

Welcome to Scribd!

  • Lampiran Kuantifikasi Revisi

    Uploaded by

    Aukoda Yuuki
    6 views5 pages
    The document discusses methods for calculating the most probable number (MPN) of microorganisms in a sample. It provides two tables: 1) Table 1 shows the results of MPN observations with different sample dilutions, recorded tube reactions, and calculated MPN values. 2) Table 2 shows the results of direct microscopic counts (DMC) with the total cell counts from three large squares for each sample, the average count, and calculations to determine the number of cells per ml of sample. The document provides methods and results for experimentally determining the concentrations of microorganisms in different water samples using MPN and DMC techniques.

    Original Description:

    Original Title

    Lampiran Kuantifikasi revisi

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document
    The document discusses methods for calculating the most probable number (MPN) of microorganisms in a sample. It provides two tables: 1) Table 1 shows the results of MPN observations with different sample dilutions, recorded tube reactions, and calculated MPN values. 2) Table 2 shows the results of direct microscopic counts (DMC) with the total cell counts from three large squares for each sample, the average count, and calculations to determine the number of cells per ml of sample. The document provides methods and results for experimentally determining the concentrations of microorganisms in different water samples using MPN and DMC techniques.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    6 views5 pages

    Lampiran Kuantifikasi Revisi

    Uploaded by

    Aukoda Yuuki
    The document discusses methods for calculating the most probable number (MPN) of microorganisms in a sample. It provides two tables: 1) Table 1 shows the results of MPN observations with different sample dilutions, recorded tube reactions, and calculated MPN values. 2) Table 2 shows the results of direct microscopic counts (DMC) with the total cell counts from three large squares for each sample, the average count, and calculations to determine the number of cells per ml of sample. The document provides methods and results for experimentally determining the concentrations of microorganisms in different water samples using MPN and DMC techniques.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 5

    LAMPIRAN

    1. Perhitungan MPN
    1
    𝑁𝑖𝑙𝑎𝑖 𝑀𝑃𝑁 = 𝑛𝑖𝑙𝑎𝑖 𝑀𝑃𝑁 𝑡𝑎𝑏𝑒𝑙 ×
    𝑃𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛 𝑡𝑎𝑏𝑢𝑛𝑔 𝑦𝑎𝑛𝑔 𝑑𝑖 𝑡𝑒𝑛𝑔𝑎ℎ

    𝑃𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛 𝑡𝑎𝑏𝑢𝑛𝑔 𝑦𝑎𝑛𝑔 𝑑𝑖 𝑡𝑒𝑛𝑔𝑎ℎ = 10−2


    Tabel 1 Hasil Pengamatan Uji MPN
    Hasil Nilai Nilai Hitung
    Kel Tabung Gelembung Dokumentasi
    Pengenceran MPN MPN

    a +
    1:10 b + 3
    c +

    a + 0,21 × 1
    =
    1 1:100 b + 2 0,21 10−2
    c - = 21

    a +
    1:1000 b + 2
    c -
    Hasil Nilai Nilai Hitung
    Kel Tabung Gelembung Dokumentasi
    Pengenceran MPN MPN

    a +
    1:10 b - 2
    c +

    a + 0,42 × 1
    1:100 b - 2 =
    2 0,42 10−2
    c +
    = 42

    a +
    1:1000 b + 3
    c +

    a -
    1:10 b + 2
    c +

    0,35 × 1
    3 0,35 =
    10−2
    = 35

    a +
    1:100 b - 2
    c +
    Hasil Nilai Nilai Hitung
    Kel Tabung Gelembung Dokumentasi
    Pengenceran MPN MPN

    a +
    1:1000 b - 2
    c +

    a +
    1:10 b + 3
    c +

    a + 24,00 × 1
    =
    1:100 b + 3 10−2
    4 >24,00
    c + = 2400

    a +
    1:1000 b + 3
    c +
    Hasil Nilai Nilai Hitung
    Kel Tabung Gelembung Dokumentasi
    Pengenceran MPN MPN

    a -
    1:10 b + 2
    c +

    0,34 × 1
    a - =
    5 0,34 10−2
    1:100 b - 1 = 34
    c +

    a +
    1:1000 b + 3
    c +

    a +
    1:10 b + 3
    c +

    24,00 × 1
    =
    6 >24,00 10−2
    = 2400

    a +
    1:100 b + 3
    c +
    Hasil Nilai Nilai Hitung
    Kel Tabung Gelembung Dokumentasi
    Pengenceran MPN MPN

    a +
    1:1000 b + 3
    c +

    2. Direct Microscopic Count (DMC)

    Tabel 2 Hasil pengamatan uji DMC


    Jumlah sel tiga
    Kelompok Perhitungan ke-
    kotak besar
    1 178
    1
    2 135
    1 135
    2
    2 141
    1 134
    3
    2 122
    1 133
    4
    2 137
    1 152
    5
    2 143
    1 152
    6
    2 163
    Total 1725
    Rata-rata 143
    16
    𝐽𝑢𝑚𝑙𝑎ℎ 𝑠𝑒𝑙 𝑝𝑒𝑟 𝑘𝑜𝑡𝑎𝑘 𝑏𝑒𝑠𝑎𝑟 = × 𝑟𝑎𝑡𝑎 − 𝑟𝑎𝑡𝑎 𝑗𝑢𝑚𝑙𝑎ℎ 𝑠𝑒𝑙 𝑡𝑖𝑔𝑎 𝑘𝑜𝑡𝑎𝑘 𝑏𝑒𝑠𝑎𝑟
    3
    16 1
    𝐽𝑢𝑚𝑙𝑎ℎ 𝑠𝑒𝑙 𝑝𝑒𝑟 𝑘𝑜𝑡𝑎𝑘 𝑏𝑒𝑠𝑎𝑟 = × 143 = 763 𝑝𝑒𝑟 𝑚𝑙
    3 1.250.000
    𝐽𝑢𝑚𝑙𝑎ℎ 𝑠𝑒𝑙/𝑚𝑙 𝑐𝑜𝑛𝑡𝑜ℎ = 𝑗𝑢𝑚𝑙𝑎ℎ 𝑠𝑒𝑙 𝑝𝑒𝑟 𝑘𝑜𝑡𝑎𝑘 𝑏𝑒𝑠𝑎𝑟 × 1,25 × 106
    𝐽𝑢𝑚𝑙𝑎ℎ 𝑠𝑒𝑙/𝑚𝑙 𝑐𝑜𝑛𝑡𝑜ℎ = 763 × 1,25 × 106
    𝐽𝑢𝑚𝑙𝑎ℎ 𝑠𝑒𝑙/𝑚𝑙 𝑐𝑜𝑛𝑡𝑜ℎ = 9,5 × 108 /𝑚𝑙

    You might also like