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ei duy nhdt cho 6nh xq ph6n hinh til da tqp Kahler ttdy vio IP^(C) .............. 3
3. Trin Vin Ngh!, Vd tinh li6n tr;c cOa him gi6 tri t6i rru trong biritoiin quy hoach
toin phuong kh6ng l6ichrla tham s6........ ...............................23
4. Trdn MEnh Cudng, Md rQng bing tdn lim vi6c c0a anten loa virng s5ng wifi
bing c5ch sr? dr;ng v6t ligu metamateria!..................--. ..........31
5. Nguy6n Th! Hiin, Nguy6n Xuin Ca, NguyEn Tlri May, Phlm Minh Tin,
Nguy6n Thanh Ttng, V0 Dinh Lim, Vai trb crla tdn hao t6p trGn m6i !€n sr,t md r6ng virng
c6 chidt sudt 6m sff dung m6 hinh lai hod bac hai ........... a..a.a.aaaa.a....aala....aa..aaac.a..a. ...... a. a.40
6. ,Hir Thanh H&ng, Bi6n d0 tiin xa toirn phdn c0a qud Binh ri h + pr trong m6 hinh 2ee..................51
7. V[ Thanh Tri, Virendra Kumar Verma, Di6u khi6n ttQ d5n tlifn t?i bd mit di chdt
8. Trlnh Khinh Son, Nguy6n Thu! Linh, Sinh trrrdng tgdudng c0a vitio
Chlorellavulgaristr€n hq thdng bd quang hgc d4ng tdm phing srldgng kithu{t
nu6i cdy m4 b6 sung co chdt vi b6 sung co chSt tap lai.-......... ..................70
9. Dudng Th! Thuf Hing, Dac eti6m hjnh tugng nh6n vit tr6 em trong ?p
..
Nh1ng truy€n hay vidt cho thidu nhi crla Ma VEn Kh5ng ................................79
10. Bti ThuI Linh, Hidn tdtrong Ddn Huong Hinh (Mec Ng6n).-** ........90
11. Nguy6-n Thu Hrrong, Ling tr6nh - M6t cSch ti6p nhin Idi k*ren c0a ngudi Nga vi ngudi ViQt
trong vin hoii giao tidp ............... ...................... 101
12. teThiLanAnh,Thi6tkdtrdchoi6chcrthinhngrJ,tucngochohgcsinhti6uhoc.........................11o
13. Phgm Kim Chung, NguyEn Qudc Huy, Thidt kg chd tao thi6t b! thi nghi6m
vd srJ quay kh6ng efdng b0, dQng cd kh6ng efdng b0 ba pha
trong day hgc vat li d trudng trung hgc phd thdng........ ............1 1g
14. NguyEn Thi Thu Lan, Xiy dung bQ c6ng cr,r ddnh giii ning lgc tU hqc
cOa sinh vi€n trudng Cfqi hgc srr phqm trong day hgc m6n Hoii hoc tfai cudng 1..............................121
1 5. Nguy6n Vin Tirng, Dd xu6t bO tieu chl cliinh giii siich gi6o khoa Ngil vin
theo Chr.tong trinh giiio duc phd th6ng m6i.... ......,..............136
70 ll TRUONeDAT Hoc su PHAM HA ruOr z
T6m tdt: Chloretla vulgaris dry nuOi ttt dudng trong bd nuOi quang h* dang tdm
phdng (FPPB, flat-ptate photobioreactor) s* dung Ki thuQt nuOi cdy nE @C, batch),
b6'sung co chdt fB, fed-batch) vdt b6 sung co chdt li6n tuc (RF, repated fe&
batch) voi th| tich hoat dOng t6i da 601. Giai doqn BC duqc thw hiln vdi 201. TEp
theo, hai quy trinh sE dt-w.c 6p dUng. (a) quy trinh X1.0, 201 mOifitutg m6i dtryc
b6 sung thdnh tirng dqt (FB) vdo FPPB cho ddn khi dat 601; sau fr, d giai tuqn
RF, 2Ol dlch nuOi duqc l1y ra vdt thay bd1g 2Ol m6i trudng mil. (b) quy tinh X0.5,
101 m6itndng mOi &rc b6 sung thdnh t*ng dqt (FB) vdo FPPB dp dn *hi dat
601; sau d6, d giai doAn RF, 201 dich nuli dttW tdy ra vd thay bdng 2Ol mili turtng
moi. Cdc Xdt qud cho thdy rdng cdc th1ng s6 ve niCu sudt sr? dUng bd mdt chi6u
sdng cha FPPB vd hieu sudf sri, dqtng co cndt de ho sdn phdm d,qc xep neo tnO
tV BC < FB < RF. Cdc giai doqn RFs cha quy trinh X0.5 cho X6t qud nua t6t nhdt
thOng qua cdc th6ng s6 v6 sdtn ltryng vd hiQu sudt.
T* khod: Chlore\a vulgais, nuOi cdy md, nuOi cdy bd sung co chilt, nuei cdy bd
sung co cndt Ep lai, sinh tntilng tq dudng
1. MO DAU
Chlorela vulgaris li mQt 1o4i vi t6o dugc nghiOn cr?u dr)ng A6 san xu6t nguOn sintr
ttri5i cO gi6 tri cho sric khoe [].
Nu6i c6y vi tao b6ng hg th6ng mO cAn di€n tich sri dung
r6t ton vi r6t aE nniSm c6c lo4i sinh vft kh6c. HQ thSng nudi quang hqc krn, c6 th6 ttat
du-o. c hiQu qui chuyi5n ho6 cao vi dE ki6m so6t (nhiet dQ, cti6t dinh duong...). He th6ng
bC nu6i quang hgc d4ng 15* phe"g (FPPB) c6 uu ditim so vor cfuc hS th6ng nu6i quang
hgc kin kh6c: di$n tfch bA mat duo. cpht hqp v6i nu6i c6y ngodi toi,
chi6u siing 16m,
c6 th6 sri dung cho tao ttugc c5 dinh, 6nh sang dE dang xuy6n qu4 san luqng t4o
euy trinh X1.0: Sau btin ngiy nu6i c6y BC, m6i trudrng Walne (20I) iluo. c bO sung
vio FPPB (giai ttoan FBI). Sau 7 ngiy tiiSp theo, m6i trulng (20| tiep tgc duo. c cho vio
FppB (giai tlo4n FBz). Sau l0 ngny tiiSp theo, qu6 fiinh RF ttuqc bit diu. Voi mdi giai
tto4n RF (RFI-RFS), dich nu6i c6y (20I) ttuo. c l6y ravi mQt th6 tictr m6i trucmg tuong
duong itugc bO sung.
TRUONe DAI Hgc su PHAM HA nQl z
Quy trinh X0.5: Sau bi5n ngiy nu6i cdy BC, m6i tru&ng V/alne m6i (l0f hn tuqt
(til giai do4n FBl dtin FB4) ttu-o. c b6 sung vio FPPB. C6c giai do4n RFI-RF3 tluqc bit
-l ! ! 4 - nE l:-t-
<tdu tu ngiy nu6i cAy thri 13. Voi m6i giai do4n RF, dich nu6i
- (20I) ituqc ldy ru vd mQt
the tich m6i trudng Walne tucrrg duong ttuqc b6 sung vdo FPPB.
2.1.2. Xdc dinh him lUQng chlorophyll
Hdm lugng chlorophyll a (Ca), chlorophyll b (Cb) vd chlorophyll t6ng si5 (Ct) duqc
tfnh toan dlra vio phuong ph6p cria Junhui Zhotvi cQng sU [5].
d4r sau ba ngiy nu6i. Tuy nhi€n, gi6 U DCW-u,r det cyc d4r o ngiy thi 5. Nhu vfy, sy
gia tiing s,5 hqng vd lfiiSi hgng t5 Uao di6n ra kh6ng d6ng thoi. Pha sinh tnr&rrg 6n dinh
diSn ra tu ngiy thi 3 - 6, theo d6, pH.r. d4t dugc d cu6i pha 6n dinh" tlinh 24 cho
th,5y, him lugng chlorophyll (Ca, Cb vd Ct) dat cUc d4i t4l ngny thri 4 vi dry ni 6n ttinh
di5n cu6i giai tloqn BC. Sau d6, loglrl, DCw vi pH gi6m nhanh ch6ng do sinh tru&ng
cira vi tio bu6c vio pha suy vong.
Quy trinh Xl.O (Hinh 1B vi 2B): Sau BC, FB1-FB2 duqc ti6n hinh a6n aiSn the tictr
ho4t ilQng toa" tU dAt40vi 60I; do s.upba lo6ng; ptl, logl.t, DgWvahim lugng chlorophyll
giem dQt ng6t. Tuy nhi6n, cac giltri nay nhanh ch6ng ting tr& ler. O cuiSi cric FB, logN
vd DCW cho th6y, s1r sinh tru&ng cria C. vulgaris d c6c giai tto4n FB t6t hqn so voi giai
do4n BC. Circ giaL<loan Ml-nfS bit ttAu tir ngdy thri 10. CuiSi mdi giai do4n RF, logN
*
TAP cHi KHoA Hoc - s6 st tool2ol7)
vi DCW gi6m so vcri gi6 tri tucvng rmg & c6c FB. O cul5i RF5, gi6 fi pHn,* vi Ct o
gi6m so v6i c6c gi6 ti tuong ung 0 cu6i BC vi c6c fg. Can hai ngiy eC eat gi6 ti
cgc t14i cria logN, DCW vi him lugng chlorophyll rong giai do4n RF; trong ktri d6 c6n
3 - 4 ngity d giai tto4n FB vi BC de gi6 d tuong t.u.
7.4 1.E
9.5 1.6
7.2
1.1 s{Xi
9.0
7.0
12
z E.5 1.0 4{}0
Ngiv
7.4
7.2
7.0
z
ot
6.8
-3
6.6
6.4
10 12 14 16 18
Ngiv
Hinh 1: 56 luqng td bdo vi tdo (logN), pH Hinh 2: Hirn luong chlorophyll (Ca, Cb vd Ct)
vd hdm luqng nitd (o/oN) cha dlch nu6i C, vulgaris vd sinh khdi td bdo kh1 (DCW) cha dlch nu6i
trong (A) nu6i cdy me, B) quy trinh Xl .0 C.vulgaris trong (A) nu6i cdy ffi{
vd (C) quy trinh X0.5. (B) quy trinh Xl .0 va (C) quy trinh X0.5.
Quy trinh X0.5 (Hinh 1C vd 2C): Sau BC, FB1-FB4 ducr. c Uit AAu hm th6 tich ho4t
dQng tu&r tp dat 30 - 60/. Do sg pha lodng, pH, logN, DCW vd hdm luqng chlorophyll
giam dQt ngQt. Tuy nhi6n, citc gi6 tri ngdy nhanh ch6ng tlng trd lai. Gi6 pH,,* t?i ti
cutii m6i giai tto4n BC vi FB vio khoang 9.2 - 9.5. logN, DCW vi him lu-o,ng
chlorophyll (Ca, Cb vd Ct) cu5i FBs d6u cao hon cic gi|tri tucmg ung 0 BC. B6n c4nh
d6, hem luqng nito tai cu6i m5i giai do4n FB dOu hn tuqt gif,m cho th6y sg sinh tru6mg
cltra C. vulgaris 6 cfuc FB t5t hon so voi BC vi hiQu qui sri dqng co ch6t cao & FB, d{c
biet FB3-FB4. Sau FB4, giai tto4n RF duo. c kfioi tlQng. CAc gii; fi logN-,. vi DCW.*
cira cdc RF kh6 tuong d6ng v6i chc gi6 E tuong trng o c6c FB. Tuy nhi6n, pH.* vi
him luqng chlorophyll cyc d4i O cuiii c6c RF lei th6p hcvn 0 c6c FB.
TRUONG
nitrate hodc urea sE dem lai hiQu qui t6t hon viQc srl dgng amonia cho sg ph6t tri6n sinh
nrOi va tich lu!
ch6t b6o 6 C. vulgaris l2l. Khi quang t.u du6ng, t6c dQ sinh trucrng cria
t6o li6n quan mflt thi6t d6n thdi gian chi6u sang vd cucrng dQ chi6u s6ng [12]. Quang
t6ng hgp bao g6m hQ thdng quang hod l vd hQ th6ng quang ho6II. Ti 16 chlorophyll a/b
tdng khi tdng cucmg i10 chi6u sang. Khi ti lQ niy bitg 2,0 thi he th6ng quang ho6 II
d5ng vai trd chinh vi khi ti lQ ndy h 6 thi hQ th6ng
quang ho6I d6ng vai trd chinh [13].
Trong nghiOn cr?u niy, tirc Calcb < 2,0 chimg t6 cudmg d0 chitiu s6ng trong nghiCn cr?u
chua hoAn toan t6i uu cho sg ph6t hirSn cua C. vulgaris.
Trong nghi6n ciru ndy, thoi di6m bit dAu FBs, RFs ld hic iogN d4t cuc dai. C6c
nghi6n criu cho th6y, vi tio khi duo. c thu ho4ch & cuiSi pha 1og c6 hdm luqng protein,
lipid vi carbohydrate ldn luqt vio klroing 30 - 4OoA, l0 - 20% vi 5 - 15%. A c6rc giai
dopn khdc, c5c thinh ph6n nny sE c6 sy thay d6i kh6c nhau [2]. Trong quy trinh Xl.0 vi
X0.5, citc gi| tri logN-*, DCW.r* vi him lugmg chlorophyll cuc dai, cdn cr? vio giai
doan nu6i c6 thO sip x6p theo thri t.u BC < RF < FB. Nhu vfy, FB c6 higu qui nh6t
DCW-* vi him luqmg
trong vi6c thu nhfln sinh khi5i vir chlorophyll. Hcm ntia, logNrarK,
chlorophyll cFc d4i c6c FB vit cbc RF ctra quy trinh X0.5 t10u cao hcrn so v6i c6c gi|tri
tuong wrg o quy trinh X1.0. Didu niy c6 th6 duo. c gi6i thich do boi d quy trinh X0.5,
lugmg m6i trucrng moi th0m vio 0 giai tlo4n cic FB Ii it hcm so v6i o quy trinh X1.0.
girip han ch6 sg thay d6i clQt ngQt thenh phan noa hgc trong m6i trubng, tt d6 aan A6n
sg sinh truong cria vi tio ttSt hon. Hcrn nta, trong c6c RF, lugng m6i truong m6i thay
thi5 chitSm lB tlngth6 tich m6i truong nOn cflng adn A6n ktit qun tuong tg. Tuy nhi6n,
c6c FB vir c6c RF tt6u cho k6t qua tOt hcyn BC do trong hQ thting nu6i c6y dd c6 sin mQt
luqmg lcm vi tio tru6c m5i giai do4n ndy.
> - s6 sr t.:,o/zor7) ll 7E
Theo Bang l, gi6 fri VP vd P c6 thf t.u nhu sau FBI < BC < FB2 (< FB3 < FB4)
voi ci hai quy trinh X1.0 vi X0.5. DiAu ney chrmg t6 s6n lugng sinh kh5i tpo thanh 0
giai ttopn dAu cua FB cao hon BC nhd luqng co ch6t moi duo. c b6 sung vi sp pha lo6ng
hm giam cdc ylu t6 Adt lgi cho sinh trucmg cria vi tao. Tuy nhi6n, & cac FB titip theo,
luqng co ch6t b6 sung vd sg pha lo6ng chua duo. c t5i uu ddn tt6n kfin rnng tao sinh kh5i
cria vi t6o giftm aan. f6t qu6 cria dA tei kfi6 tucmg d6ng voi nghiOn ct?u cria Renato
S. Coelho vi cQng sg [14], theo d6, ldthuAt nu6i FB glup d4t DCW,,,. vi P,,,.so voi BC.
Ngodi ra, trong qu6 trinh nu6i vi tho Spirulina platensis, Marcelo C. Matsodo vir cQng
sy [15] ciiirg cho thdy viQc 6p dUng ki thu$t RF grup fing gi|tri DCW P, vi YpTs so vtvi
ki thu4t BC. Khi tlffi gi6 vC hiQu suSt srl dgng be mlt ttugc chitiu sAng (ISP) vd hiQu
suAt t4o thenh san phAm (Ypis) thi cdc FB 1u6n cao hcrn so voi BC. O quy trinh X1.0,
RFs mflc dir c6 gi6fri VP vd P nh6 hon d FBI vi BC. Nhmg v6i quy trinh X0.5, gi6 tri
VP cria c6c RF 14i cao hon 6 BC. ThOm vio d6, gi|txi ISP vi Yprs cria c6c RF lu6n cao
hon c6c gi6 tri tuong ung o BC vi c6c FB.
Bdng l: Cdc th6ng sd sinh trudng ctta vi tdo ffen cdc giai iloqn vd quy trinh khdc nhau
15P (mg.m'.d-') 20,0u 50,7d 42,7b 45,0, 543h 53,6s 53,1' 53,4t
Y,ru (%) 39,9', 66,9s 52,5d 43,6b 52,6' 52,5d 52,7 52,4'.
15P (mg.m'.d-') 20,0' 33,9* 32,0d 28,ff 23,9b 57,9t 63,6h 63,3s
Yprs (%) 39,9d 78,2s 39,4', 29,6b 19,3', 52,9f 52,91 52,6'
Cdc chii khdc nhau trong cimg mft hdng cho bidt sU kfuic bi€t cd nghta vi mrtt
thiing kA @ < 0,05); VP: laqng sinh ffiiit (mg) tqo thdnh tuAn m\t ilon vi the fich (t)
trong mAt ilan vi thdi gian (ngdy); ISP: luqng sinh khiii (mg) tqo thdnh ffAn m\t ilon vi
bd mqt he thning du.oc chiilu sdng (m2) trong m\t donvi thiri gian (ngdy); P: laqng sinh
khdi 1mg) tqo thdnh trong m\t donvi thdi gian (ngdy); Y: hi\u sudt tso thdnh sdn phdm,
fi rc (n giila luqng sinh khiii (mg) tqo thdnh ffAn luqng co chiit U fieu tha fufl.
TRudNe@supHAM HA ruQt z
3. rEr luiu
Tt a6 ur6y ring, c6c th6ng s5 vd san lugng (vp, p) d
cric ttSt qua thu nh4n duo. c c6
FB & ci hui quy tinn Adu k6m hon giai tfo4n BC. xdt vA hi6u srr.it sri dgng co ch{t tao
san phAm (Yprs) 6 giai <topn FB thi quy tlnh xl.O cao hon x0.5. Nhin chung, voi quy
fiinh x0.5, c6c y6u td vA san lugng (vp, p) vi hiQu sudt gsr, ypls) cta giai clopn RFs
ttAu ttit hon c6c gi6 tri tuong tmg d giai do4n BC vi FBs. c6 th6 th6y r5ng, & pps theo
quy trinh X0.5 sE cho c6c th6ng s5 nu6i t6ng t5t nh6t so vor clrcgrai do4n Hriic tong ci
hai quy trinh tluqc nghiOn cfu. C6c ktit qua cria nghiCn criu niy girip crlc nhi nghi6n cr?u
hoflc san xu6t sinh kh6i tao c6 du-o. c c6c th6ng s6 dA tya chgn vi di6u chinh nh6rn thu
tluoc k6t qua t6t nh6t.
1. Amini Khoeyi 2., Seyfabadi J. and Ramezanpow Z. (2011), Effect of light intensity
and photoperiod on biomass andfatty acid composition of the microalgae Chlorella
vulgaris, Aquaculfure International, 20, pp. 4l - 49 .
2. Giovanni Antonio Lutztt Q}ll), Analysis of the growth of microalgae in batch and
semi-batchphotobioreactors, PhD Thesis, Universita degli studi di cagliari.
3. stanbury P. F., Whitaker A. and Hall s.J. (2003), pt.inciples of Fermentation
Technol ol gt. Butterw orth Heinemann, Elsevier Science Ltd.
4. Walne PR. (1970), Studies on the food value of nineteen genera of algae to juvenile
bivalves of the genera ostrea, Crassostrea, Mercenaria and Mytilus, Fishery
investigations, Great Britain Minisby of Agriculture, Fisheries and Food, H.M.
Stationery Office.
-
J. Junhui Zhou" Feifei Qin, Jie Su, Jian-wu Liao and HuiJian Xu (201l), PuriJication
of formaldehyde-polluted air by indoor plants of Araceae, Agovaceae and
Liliaceae, Joumal of Food, Agriculture & Environment, 9, pp. l0l2 - l0lg.
6. Mahendraperumal Guruvaiah, Keesoo Lee Q0l4), Effect ofJtue gcrs on microalgae
population md study the heavy metals accumulation in biomass
from power plant
system,Intemational Joumal of Applied Sciences and Biotechnolgy,z,pp. l 14
-120.
7. AACC, Internotional Approved Method of Analysis I lth Ed., AACC International,
St. Paul, MN, U.S.A.
8. Rachlin J.W., Grosso A. (1991), The fficts of pH on the growth of Chloretta
vulgaris and its interactions with cadmium toxicity,Arch Environ Contam Toxicol,
20,pp.505 -850.
) . 56 sr fiotzor7) ll tt
9. Espen Granum and Sverre Myklestad (2001), A photobioreactor with pH
M.
control: demonstration by growth of the marine diatom Skeletonema costatum,
Journal of Plankton Research, 24,pp.557 -563.
10. Sobczuk T. M., Camacho F.G., Camacho Rubio F., Acien Fernandez F.G. and
Molina Grima E. (2000), Carbon dioxide uptake efficiency by outdoor microalgal
cultures in tubular air-firt photobioreactors, Biotechnology and Bioengineering,
67,pp.465 -475.
11. Mandalam R. K. and Palsson B.O. (1998), Elemental balancing of biomass and
medium compositioru enhances growth capacity in high-density Chlorella vulgaris
cultures, Biotechnology and Bioengineering, 59, pp. 605 -611.
12. Pirt, J. S. (1986), The thermodynamic eficiency (quantum demand) and dynamics
of photo synthetic gr owth,New Phytologist, 102, pp. 3 - 37 .
13. Bonnie J. Reger and Robert W. Krauss (1970), The Photosynthetic Response to a
Shifi in the Chlorophyll a to Chlorophyll b Raio of Chlorella, Plant Physiol, 46,pp.
s68
-575.
L4. Renato S. Coelho, Annamaria D. S. Vidotti, Brita M. Reis, Telma T. Franco
(2014), High Cell Density Cultures of Microalgae wtder Fed-batch and Continuous
Growth, Chemical Engineering Transactions, 38, pp. 313 -318.
15. Marcelo C. Matsudo, Raquel P. Bezerr+ Sunao Sato, Patrizia Perego, Attilio
Converti, JoSo Carlos M. Carvalho Q009), Repeated fed-batch cultivation of
Arthrospira (Spirulina) platensis usrng tfrea as nitrogen source, Biochemical
Engineering Journal, 43,pp. 52 -57.
in RF sfages, 201 of broth was replacr,d by the same volume of fresh medium.
Values of illuminated surtace produdivi$ (PS) and yied Aprd were ordered as
BC < FP < RF. RF sfages of X0.5 prooess showd fhe besf result based on values
of yield and volumefic productivity (P)-
Keyttords: Chlorella vulgais, batch anlfite, f&batch dtlture, repeated-fed batch
culture, heterotrophic cufturc
(Ngdy Tod soan nhQn dtryc bdi: 31-7-2017; rvdy phdn bi€n ddnh gidt: 04'9-2017;
ngdy chdp nhdn ffing: 12-9-2017)