GAATTC_GCCACC_MALPVTALLLPLALLLHA_CRGDKGPDC_KIVMTQTPATLSVSAGERVTITCRASQSVSNHVTW

YQQKPGQAPRLLIYSASNRYTGIPARFSGSGYGTEFTFTISSVQSEDFAVYFCQQDYSSFGQGTKLEIKRGGGGSGG
GGSGGGGSQVQLVESGPGVVQPGRSLRLSCAVSGFSVTNYGVHWVRQPPGKGLEWLGVIWAGGITNYNSSVKG
RLTISKDNSKNTVYLQMNSLRAEDTAVYYCASRGGHYGYALDYWGQGTLVTVSSGGGGSGGGGSGGGGSEVQL
VESGGGLVKPGGSLRVSCAASGFTFSSYAMSWVRLAPGKGLEWVATISSAGGYIFYSDSVQGRFTISRDNAKNSLY
LQMNSLRAEDTAMYYCARQGFGNYGDYYAMDYWGQGTLVTVSSGGGGSGGGGSGGGGSDIELTQSPSSLAVSA
GERVTMNCKSSQSLLNSRTRKNQLAWYQQKPGQSPELLIYWASTRQSGVPDRFSGSGSGTDFTLTISSVQAEDVA
VYYCQQSYNLLTFGPGTKLEIKR_AAAPTTTPAPRPPTPAPTIASOPLSLRPEACRPAAGGAVHTRGLDFACDIYIWAPL
AGTCGVLLLSLVITLYCN_KRGRKKLLYIFKQPFMRPVQTTQEEDGCSCRFPEEEEGGCEL_RVKFSRSADAPAYQOGON
QLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELOKDKMAEAYSEIGMKGERRRGKGHDGLYOGLS
TATKDTYDALHMOALPPR_TAA_GSGEGRGSLLTCGDVEENPGP_MFHVSFRYIFGLPPLILVLLPVASSDCDIEGKDG
KQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRAARKLRQFLKMNSTGDFDLHLLKVSE
GTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSLKEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH_TAA_GS
GEGRGSLLTCGDVEENPGP_MALLLALSLLVLWTSPAPTLSGTNDAEDCCLSVTQKPIPGYIVRNFHYLLIKDGCRV
PAVVFTTLRGRQLCAPPDQPWVERIIQRLQRTSAKMKRRSSLALLLALSLLVLWTSPAPTLSGTNDAEDCCLSVT
QKPIPGYIVRNFHYLLIKDGCRVPAVVFTTLRGRQLCAPPDQPWVERIIQRLQRTSAKMKRRSS_TAA_ACTAGT

GAATTC

GCCACC

CD8 signal peptide:ATGGCATTGCCTGTGACAGCTTTGTTGTTGCCATTGGCCCTTCTTCTGCACGCT

IRGD peptide:TGCAGGGGCGATAAGGGACCCGACTGC

antiGD2_MUC16_scfv:AAGATTGTTATGACTCAGACTCCCGCCACCCTGTCCGTTTCTGCAGGGGAAAGAGTTACCAT
CACTTGCAGGGCTTCCCAGTCTGTCAGCAATCACGTCACCTGGTACCAGCAAAAGCCAGGACAGGCACCACGGCTG
TTGATTTATTCTGCTTCCAACCGATACACCGGCATCCCAGCACGGTTCAGCGGTTCTGGATATGGGACAGAGTTCAC
TTTTACTATCTCCTCTGTGCAGTCTGAGGATTTCGCCGTATACTTTTGCCAACAAGACTATTCATCTTTTGGACAAGG
GACGAAGCTGGAAATAAAAAGGGGTGGCGGAGGATCTGGTGGAGGAGGATCAGGAGGAGGTGGCTCTCAAGTGC
AGCTCGTAGAAAGTGGACCGGGAGTCGTGCAACCAGGCAGAAGTCTGAGGCTTTCCTGCGCCGTTAGTGGATTCAG
TGTGACAAATTACGGTGTGCATTGGGTGAGACAGCCTCCAGGAAAGGGGCTGGAGTGGCTGGGAGTGATCTGGGC
AGGTGGAATCACTAATTACAATAGTTCCGTTAAGGGAAGACTGACTATTTCTAAGGATAATAGTAAGAACACCGTT
TACTTGCAGATGAACTCCCTGAGGGCGGAGGACACAGCAGTGTATTACTGTGCTAGCAGGGGCGGACACTACGGAT
ACGCTCTGGACTACTGGGGTCAGGGCACCTTGGTGACAGTCTCTAGTGGAGGCGGGGGATCTGGAGGAGGAGGTTC
AGGAGGAGGTGGGAGTGAGGTGCAACTGGTCGAAAGCGGCGGAGGGCTTGTTAAACCTGGCGGATCCTTGCGGGT
TTCTTGCGCTGCATCCGGATTCACATTTAGCAGTTACGCCATGTCATGGGTGAGATTGGCCCCAGGCAAAGGGCTCG
AATGGGTGGCTACAATTTCAAGTGCAGGGGGATACATCTTCTACAGCGACAGCGTCCAAGGCAGGTTCACGATTTC
TAGGGACAATGCGAAAAACTCCCTGTATCTGCAGATGAACTCCCTCAGAGCCGAAGACACAGCCATGTATTACTGT
GCTAGACAGGGCTTTGGGAATTACGGAGATTACTACGCCATGGACTATTGGGGACAGGGCACGCTGGTGACCGTCT
CATCCGGTGGTGGCGGATCAGGAGGAGGAGGTTCAGGTGGAGGAGGCAGTGACATTGAGCTCACCCAATCCCCTT
CTTCCTTGGCAGTGTCCGCCGGGGAAAGAGTCACCATGAACTGTAAGAGTTCCCAAAGCCTCCTCAATAGCAGAAC
ACGCAAGAATCAGTTGGCCTGGTACCAACAGAAGCCTGGACAGAGCCCCGAACTTCTCATCTACTGGGCAAGTACC
AGACAGTCCGGTGTTCCAGATCGGTTTAGCGGCAGCGGTTCAGGCACCGACTTCACACTTACAATTAGCTCTGTGC
AAGCAGAGGACGTGGCAGTTTACTACTGCCAGCAATCATACAATCTGCTGACATTCGGGCCGGGGACTAAGCTCGA
AATCAAGCGC
CD8ahinge&TM:GCTGCTGCTCCTACTACAACTCCAGCTCCAAGACCACCAACACCAGCCCCTACAATAGCTAGTCCA
CTGAGCCTGAGGCCAGAAGCTTGTCGGCCTGCTGCTGGAGGAGCAGTTCATACAAGAGGGTTGGATTTCGCCTGCG
ATATCTACATTTGGGCACCATTGGCTGGCACTTGCGGGGTGCTTCTGCTGTCCCTGGTCATCACACTGTATTGTAAT

CD137:AAAAGGGGCCGCAAGAAGCTCCTCTACATTTTCAAACAGCCATTCATGAGACCTGTGCAGACAACCCAGGA
GGAAGACGGATGCAGTTGTCGCTTCCCTGAGGAAGAGGAAGGCGGGTGCGAACTG

CD3zeta:AGAGTGAAATTTAGTAGATCCGCAGACGCCCCAGCCTATCAGGGAAATCAGCTGTACAATGAGCTGAACC
TGGGTAGGCGGGAGGAATACGATGTCTTGGACAAGAGAAGAGGCCGCGACCCTGAGATGGGAGGCAAACCAAGG
CGAAAAAACCCACAGGAAGGACTCTACAACGAACTGAAGGATAAGATGGCGGAGGCTTACAGCGAAATCGGCATG
AAGGGGGAGAGACGGAGGGGCAAAGGACATGACGGTCTGTACGGGCTGTCCACAGCCACTAAGGACACCTACGAC
GCGCTGCACATGGCACTCCCACCAAGA

TAA

P2A sequence:GGGAGTGGAGAAGGCAGAGGATCATTGTTGACCTGTGGAGATGTGGAGGAGAACCCAGGACCT

IL7:ATGTTTCATGTCAGTTTCAGGTACATCTTCGGCCTCCCCCCGTTGATTCTGGTCCTGCTGCCAGTGGCCTCCTCA
GACTGCGATATCGAGGGCAAGGATGGGAAGCAATATGAGAGCGTGCTGATGGTGTCAATTGACCAGCTGCTCGAC
TCCATGAAGGAAATCGGGAGCAACTGTTTGAATAACGAGTTCAATTTTTTCAAGAGGCATATATGCGATGCCAATA
AGGAAGGCATGTTTCTGTTCAGAGCCGCTCGAAAACTCAGGCAGTTTCTGAAGATGAATAGTACAGGCGACTTCGA
CCTGCATCTTCTGAAGGTTAGTGAGGGCACCACCATTCTTCTCAACTGTACAGGCCAGGTGAAGGGAAGGAAACCA
GCGGCCCTGGGAGAGGCCCAGCCCACAAAAAGTCTGGAGGAGAATAAATCTCTGAAAGAGCAGAAAAAGCTTAAC
GATTTGTGTTTCCTGAAGAGGCTGCTGCAGGAAATCAAAACCTGTTGGAATAAAATATTGATGGGAACGAAGGAGC
AC

TAA

P2A sequence:GGGAGTGGAGAAGGCAGAGGATCATTGTTGACCTGTGGAGATGTGGAGGAGAACCCAGGACCT

CCL19:ATGGCACTTCTGCTTGCTTTGTCTCTCCTGGTGCTCTGGACTAGCCCCGCTCCAACTCTCTCTGGGACAAATG
ATGCCGAGGACTGCTGCCTTAGCGTCACCCAGAAGCCCATCCCTGGATATATTGTAAGAAACTTCCACTACCTGTTG
ATTAAAGACGGGTGTCGGGTGCCAGCTGTGGTTTTTACCACCCTGCGAGGGAGACAGTTGTGCGCCCCACCTGATC
AACCCTGGGTTGAGAGGATTATCCAGAGACTCCAGAGAACAAGTGCCAAGATGAAGAGGAGGTCTTCCCTGGCTTT
GCTGCTGGCCCTGTCACTGTTGGTGCTGTGGACCAGTCCTGCACCAACTCTGTCCGGCACCAACGATGCTGAGGATT
GTTGTCTGTCCGTCACACAGAAGCCGATTCCCGGCTATATAGTGAGGAACTTCCACTACCTCCTGATCAAGGACGG
CTGCAGGGTTCCAGCAGTCGTGTTCACTACCCTTCGGGGCAGGCAGCTGTGTGCTCCACCTGATCAGCCATGGGTCG
AACGAATTATACAGCGACTCCAGCGCACCTCCGCCAAGATGAAAAGGCGGTCCAGC

TAA

ACTAGT

Complete sequence:
GAATTCGCCACCATGGCATTGCCTGTGACAGCTTTGTTGTTGCCATTGGCCCTTCTTCTGCACGCTTGCAGGGGCGA
TAAGGGACCCGACTGCAAGATTGTTATGACTCAGACTCCCGCCACCCTGTCCGTTTCTGCAGGGGAAAGAGTTACC
ATCACTTGCAGGGCTTCCCAGTCTGTCAGCAATCACGTCACCTGGTACCAGCAAAAGCCAGGACAGGCACCACGGC
TGTTGATTTATTCTGCTTCCAACCGATACACCGGCATCCCAGCACGGTTCAGCGGTTCTGGATATGGGACAGAGTTC
ACTTTTACTATCTCCTCTGTGCAGTCTGAGGATTTCGCCGTATACTTTTGCCAACAAGACTATTCATCTTTTGGACAA
GGGACGAAGCTGGAAATAAAAAGGGGTGGCGGAGGATCTGGTGGAGGAGGATCAGGAGGAGGTGGCTCTCAAGT
GCAGCTCGTAGAAAGTGGACCGGGAGTCGTGCAACCAGGCAGAAGTCTGAGGCTTTCCTGCGCCGTTAGTGGATTC
AGTGTGACAAATTACGGTGTGCATTGGGTGAGACAGCCTCCAGGAAAGGGGCTGGAGTGGCTGGGAGTGATCTGG
GCAGGTGGAATCACTAATTACAATAGTTCCGTTAAGGGAAGACTGACTATTTCTAAGGATAATAGTAAGAACACCG
TTTACTTGCAGATGAACTCCCTGAGGGCGGAGGACACAGCAGTGTATTACTGTGCTAGCAGGGGCGGACACTACGG
ATACGCTCTGGACTACTGGGGTCAGGGCACCTTGGTGACAGTCTCTAGTGGAGGCGGGGGATCTGGAGGAGGAGG
TTCAGGAGGAGGTGGGAGTGAGGTGCAACTGGTCGAAAGCGGCGGAGGGCTTGTTAAACCTGGCGGATCCTTGCG
GGTTTCTTGCGCTGCATCCGGATTCACATTTAGCAGTTACGCCATGTCATGGGTGAGATTGGCCCCAGGCAAAGGG
CTCGAATGGGTGGCTACAATTTCAAGTGCAGGGGGATACATCTTCTACAGCGACAGCGTCCAAGGCAGGTTCACGA
TTTCTAGGGACAATGCGAAAAACTCCCTGTATCTGCAGATGAACTCCCTCAGAGCCGAAGACACAGCCATGTATTA
CTGTGCTAGACAGGGCTTTGGGAATTACGGAGATTACTACGCCATGGACTATTGGGGACAGGGCACGCTGGTGACC
GTCTCATCCGGTGGTGGCGGATCAGGAGGAGGAGGTTCAGGTGGAGGAGGCAGTGACATTGAGCTCACCCAATCC
CCTTCTTCCTTGGCAGTGTCCGCCGGGGAAAGAGTCACCATGAACTGTAAGAGTTCCCAAAGCCTCCTCAATAGCA
GAACACGCAAGAATCAGTTGGCCTGGTACCAACAGAAGCCTGGACAGAGCCCCGAACTTCTCATCTACTGGGCAA
GTACCAGACAGTCCGGTGTTCCAGATCGGTTTAGCGGCAGCGGTTCAGGCACCGACTTCACACTTACAATTAGCTCT
GTGCAAGCAGAGGACGTGGCAGTTTACTACTGCCAGCAATCATACAATCTGCTGACATTCGGGCCGGGGACTAAGC
TCGAAATCAAGCGCGCTGCTGCTCCTACTACAACTCCAGCTCCAAGACCACCAACACCAGCCCCTACAATAGCTAG
TCCACTGAGCCTGAGGCCAGAAGCTTGTCGGCCTGCTGCTGGAGGAGCAGTTCATACAAGAGGGTTGGATTTCGCC
TGCGATATCTACATTTGGGCACCATTGGCTGGCACTTGCGGGGTGCTTCTGCTGTCCCTGGTCATCACACTGTATTG
TAATAAAAGGGGCCGCAAGAAGCTCCTCTACATTTTCAAACAGCCATTCATGAGACCTGTGCAGACAACCCAGGAG
GAAGACGGATGCAGTTGTCGCTTCCCTGAGGAAGAGGAAGGCGGGTGCGAACTGAGAGTGAAATTTAGTAGATCC
GCAGACGCCCCAGCCTATCAGGGAAATCAGCTGTACAATGAGCTGAACCTGGGTAGGCGGGAGGAATACGATGTC
TTGGACAAGAGAAGAGGCCGCGACCCTGAGATGGGAGGCAAACCAAGGCGAAAAAACCCACAGGAAGGACTCTA
CAACGAACTGAAGGATAAGATGGCGGAGGCTTACAGCGAAATCGGCATGAAGGGGGAGAGACGGAGGGGCAAAG
GACATGACGGTCTGTACGGGCTGTCCACAGCCACTAAGGACACCTACGACGCGCTGCACATGGCACTCCCACCAAG
ATAAGGGAGTGGAGAAGGCAGAGGATCATTGTTGACCTGTGGAGATGTGGAGGAGAACCCAGGACCTATGTTTCA
TGTCAGTTTCAGGTACATCTTCGGCCTCCCCCCGTTGATTCTGGTCCTGCTGCCAGTGGCCTCCTCAGACTGCGATAT
CGAGGGCAAGGATGGGAAGCAATATGAGAGCGTGCTGATGGTGTCAATTGACCAGCTGCTCGACTCCATGAAGGA
AATCGGGAGCAACTGTTTGAATAACGAGTTCAATTTTTTCAAGAGGCATATATGCGATGCCAATAAGGAAGGCATG
TTTCTGTTCAGAGCCGCTCGAAAACTCAGGCAGTTTCTGAAGATGAATAGTACAGGCGACTTCGACCTGCATCTTCT
GAAGGTTAGTGAGGGCACCACCATTCTTCTCAACTGTACAGGCCAGGTGAAGGGAAGGAAACCAGCGGCCCTGGG
AGAGGCCCAGCCCACAAAAAGTCTGGAGGAGAATAAATCTCTGAAAGAGCAGAAAAAGCTTAACGATTTGTGTTT
CCTGAAGAGGCTGCTGCAGGAAATCAAAACCTGTTGGAATAAAATATTGATGGGAACGAAGGAGCACTAAGGGAG
TGGAGAAGGCAGAGGATCATTGTTGACCTGTGGAGATGTGGAGGAGAACCCAGGACCTATGGCACTTCTGCTTGCT
TTGTCTCTCCTGGTGCTCTGGACTAGCCCCGCTCCAACTCTCTCTGGGACAAATGATGCCGAGGACTGCTGCCTTAG
CGTCACCCAGAAGCCCATCCCTGGATATATTGTAAGAAACTTCCACTACCTGTTGATTAAAGACGGGTGTCGGGTG
CCAGCTGTGGTTTTTACCACCCTGCGAGGGAGACAGTTGTGCGCCCCACCTGATCAACCCTGGGTTGAGAGGATTA
TCCAGAGACTCCAGAGAACAAGTGCCAAGATGAAGAGGAGGTCTTCCCTGGCTTTGCTGCTGGCCCTGTCACTGTT
GGTGCTGTGGACCAGTCCTGCACCAACTCTGTCCGGCACCAACGATGCTGAGGATTGTTGTCTGTCCGTCACACAG
AAGCCGATTCCCGGCTATATAGTGAGGAACTTCCACTACCTCCTGATCAAGGACGGCTGCAGGGTTCCAGCAGTCG
TGTTCACTACCCTTCGGGGCAGGCAGCTGTGTGCTCCACCTGATCAGCCATGGGTCGAACGAATTATACAGCGACT
CCAGCGCACCTCCGCCAAGATGAAAAGGCGGTCCAGCTAAACTAGT

Citations
Reinartz S, Failer S, Schuell T, Wagner U. CA125 (MUC16) gene silencing suppresses growth properties of
ovarian and breast cancer cells. Eur J Cancer. 2012 Jul;48(10):1558-69. doi: 10.1016/j.ejca.2011.07.004.
Epub 2011 Aug 16. PMID: 21852110.

Cao J, Sun S, Min R, Li R, Fan X, Han Y, Feng Z, Li N. Prognostic Significance of CCNB2 Expression in Triple-
Negative Breast Cancer. Cancer Manag Res. 2021;13:9477-9487
https://doi.org/10.2147/CMAR.S339105