Experiment #1

Screening Tests for Bloodstains

I. Objective
Determine the color reactions of a bloodstain when subjected to different preliminary
blood tests

II. Apparatus/Materials

Test tubes, spray bottle, test tube rack, ordinary filter paper, beaker (80ml), medicine
dropper, human blood stain, chicken bloodstain, pig bloodstain, fish bloodstain

III. Procedure

1. Kastle-Meyer Test

1.1. Rub a piece of filter paper on each of the questioned bloodstain.

1.2. Add one to two droplets of Kastle Meyer reagent then add two droplets of
3% Hydrogen Peroxide.

1.3. Record and illustrate your observations.

2. Leucomalachite Green Test

2.1. Follow the same procedure as in Kastle-Meyer Test.

2.2. Add one to two droplets of Leucomalachite green reagent then add two
droplets of 3% Hydrogen Peroxide.

2.3. Record and illustrate your observations.

 3. Luminol Test
3.1. Spray a freshly prepared Luminol reagent on the area of the suspected
bloodstain.
3.2. The test area must be totally dark.
3.3. Observe the color that will appear on the stained area after having been
exposed to ultraviolet light.
3.4. Record and illustrate your observations.

IV. Observation

Data and Results

Color Reactions

Leucomalachite Luminol Test

Blood Sample Kastle-Meyer Test
Green Test

1.Human Bloodstain

2.Chicken Bloodstain

3.Pig Bloodstain

4.Fish Bloodstain

V. Post Laboratory Discussion

Guide Questions

1. Kastle-Meyer Test

1.1. What is present in the bloodstain which causes to give the desired color
reaction with Kastle-Meyer Test?

1.2. What is the composition of Kastle-Meyer Test reagent?

1.3. Why is this reagent considered only a preliminary test

2. Leucomalachite Green Test
2.1. Are the colors obtained from all the bloodstains the same?

2.2. Are the Kastle-Meyer Test and the Leucomalachite Green Test the same or
different? Discuss your findings.

2.3. What is indicated if the test gives positive results with the suspected
bloodstain?

3. Luminol Test
3.1. Why is there a need to darken the room when spraying with Luminol
Reagent?

3.2. Where is Luminol Test best applied? Whydo you think so?

3.3 Why is Luminol a chemiluminiscent substance?

VI. Conclusion

VII. Illustration