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  • Materi 5

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    GABRIEL EVELIN
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    The document discusses microbial nutrition and growth. It covers the basics of microbial metabolism, the key macronutrients and micronutrients needed for microbial growth, and how microbial growth differs from growth in animals and plants. The document also examines the phases of microbial growth, methods for measuring and calculating growth, and considerations for culturing microorganisms, such as inoculation and various types of culture media.

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    The document discusses microbial nutrition and growth. It covers the basics of microbial metabolism, the key macronutrients and micronutrients needed for microbial growth, and how microbial growth differs from growth in animals and plants. The document also examines the phases of microbial growth, methods for measuring and calculating growth, and considerations for culturing microorganisms, such as inoculation and various types of culture media.

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    © All Rights Reserved
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    8 views9 pages

    Materi 5

    Uploaded by

    GABRIEL EVELIN
    The document discusses microbial nutrition and growth. It covers the basics of microbial metabolism, the key macronutrients and micronutrients needed for microbial growth, and how microbial growth differs from growth in animals and plants. The document also examines the phases of microbial growth, methods for measuring and calculating growth, and considerations for culturing microorganisms, such as inoculation and various types of culture media.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    of 9

    Materi 5

    Microbial Nutrition and Growth

    I. Nutrition and Cell Chemistry

    • Metabolism → Catabolic vs anabolic

    • Macronutrients vs micronutrients

    • An approximate chemical formula for a cell is CH2O0.5N0.15,

    C and N

    • Carbon requirements →
    • Nitrogen requirements →

    1
    Other Macronutrients

    • P→

    • S→

    • K→

    • Mg →

    • Ca →

    Micronutrients

    • Fe →
    • Growth factors →

    2
    II. Microbial Growth

    Microbial growth vs. animal/plant growth

    Growth term in microbes →

    Cell division in bacteria →

    3
    Cell Generations and Generation Time

    Quantitative aspects of microbial growth →

    4
    Calculating microbial growth parameters

    5
    Phases of growth

    Measuring microbial growth

    Direct vs Indirect methods

    6
    7
    III. Culturing Microorganism

    Inoculation →

    Culture Media

    • Defined media vs complex media

    • Nonselective vs. Enriched vs. Selective vs. Differential Media

    8
    Nonselective →

    Enriched →

    Selective →

    Differential →

    Nutrient agar

    Chocolate agar

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