ASSESSMENT

HIGH SCHOOL
AY 2022-2023
“Fostering and empowering society in building and serving the nation”

Subject : Biology Date : SCORE

Topic/s : Biological Molecule (AS Ch.2) Class : HS-10 B

Duration : 30 minutes Name :

Test for Non-reducing Sugar

(Formative Assessment)

Learning Objectives:
 describe and carry out the Benedict’s test for reducing sugars, the iodine test for starch, the emulsion test for lipids and
the biuret test for proteins

Direction: Answer the questions in the spaces provided

1 You are provided with two solutions, G1 and G2. One of the solutions contains a reducing sugar and
the other one contains a non-reducing sugar. The solutions may not be in that order.
You are required, using only the reagents provided, to identify the solutions.

(a) First, do the test for reducing sugar.

(i) State the steps of conducting the reducing sugar test.

……………………………………………………………………………………………………….
……………………………………………………………………………………………………….
……………………………………………………………………………………………………….
……………………………………………………………………………………………………….
…………………………………………………………………………………………………… [3]

(ii) State the positive observation and identify the solution containing the reducing sugar
positive observation ……………………………………………………………………………….
……………………………………………………………………………………………………….
solution containing reducing sugar …………………………………………………….……. [1]

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 ASSESSMENT
HIGH SCHOOL
AY 2022-2023
“Fostering and empowering society in building and serving the nation”

(b) Next, do the test for non-reducing sugar.

(i) State the steps of conducting the non-reducing sugar test.

……………………………………………………………………………………………………….
……………………………………………………………………………………………………….
……………………………………………………………………………………………………….
……………………………………………………………………………………………………….
…………………………………………………………………………………………………… [3]

(ii) State the positive observation and identify the solution containing the non-reducing sugar
positive observation ……………………………………………………………………………….
……………………………………………………………………………………………………….
solution containing reducing sugar …………………………………………………….……. [1]

(c) Identify two sources of error in your investigation.

1 ……………………………………………………………………………………………………………..
2 ………………………………………………………………………………………………………… [2]

[Total: 10]

END OF ASSESSMENT

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