Professional Documents
Culture Documents
TABLE OF CONTENTS
Pages
TITLE PAGE i
CERTIFICATE OF PANEL APPROVAL ii
DEDICATION iii
ACKNOWLEDGEMENTS iv
TABLE OF CONTENTS v
LIST OF TABLES vi
LIST OF FIGURES vii
CHAPTER
1 THE PROBLEM AND ITS SCOPE
1.1 Introduction 1
1.2 Conceptual Framework 2
1.3 Objectives of the Study 4
1.4 Statement of the Problem 4
1.5 Hypothesis 5
1.6 Significance of the Study 5
1.7 Scope and Delimitation 7
1.8 Operational Definition of Terms 7
3 RESEARCH METHODOLOGY
3.1 Research Design 17
3.2 Research Materials and Methods 17
3.3 Research Procedures 18
REFERENCES 24
iii
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DEDICATION
This effort is the end result of many hardships and restless nights. Thanks to
the researchers, this work is proudly and sincerely dedicated to the individuals who
serve as an inspiration for this work to happen. The information in this research
article will be useful to poultry owners, farmers, aspiring researchers, members of
the local community, educators, and business owners to their future plans and
works. To all the parents, guardians, friends, and classmates who provided support
when difficulties arose; to the entire Liceo de Cagayan University faculty and
staff; and, most importantly, to God Almighty for providing us the knowledge,
courage, persistence, strength, and time to complete this assignment.
- The Researchers
Liceo de Cagayan University Senior High School Department iv
ACKNOWLEDGEMENTS
The researchers would like to thank all the people who helped them in any ways,
small or big and had given any amount of their time to help in making their study.
The researchers would also like to express their deepest appreciation to Mr. Jas
Felicisimo A. Cane, who served as their research adviser, for his patience, effort in
teaching the researchers and his guidance for this work to be possible.
They are also extremely grateful to each of their family, who always support and
appreciate every work and everything they do. For letting them travel to other
places for meetups and discussions regarding their paper.
The researcher would also like to extend their warmest gratitude to the Aunt and
Uncle of their research leader for letting them gather papaya latex in their papaya
farm for free.
Lastly to their school, Liceo de Cagayan University, thank you for providing such
quality education through the faculties and staffs. And lastly, Our God Almighty
for giving enough wisdom, time, courage and strength to continue writing this
paper.
Liceo de Cagayan University Senior High School Department vi
LIST OF TABLES
Table Pages
1 Research Material and their Uses 17
Liceo de Cagayan University Senior High School Department vii
LIST OF FIGURES
Figure Pages
1 Schematic Diagram of the Study 3
2 Flow Chart 19
Liceo de Cagayan University Senior High School Department
A Research Proposal
Presented to the
Faculty of the Senior High School – Main Campus
Liceo de Cagayan University
Cagayan de Oro City
—------------------------------------------------------------------------------
In Partial Fulfillment
of the Requirements for the Senior High School Department
Science, Technology, Engineering and Mathematics
—------------------------------------------------------------------------------
ARCAMO, BRENT MICHAEL
BAA, NIANNE KASSANDRA
BAUTISTA, JLIAN
CAIREL, ALYZA NAOMI
CIMACIO, CHLOE DOMINIQUE
JABAYBAY, KEANE RIX
LICOT DIRK REISHI
YAP, JOHN WILSON
APRIL 2023
Liceo de Cagayan University Senior High School Department
Chapter I
1.1. Introduction
The efficacy of papaa (Carica papaya) crude latex as a sticky trap against
increasing interest in the field of pest control. Papaya (Carica papaya), commonly
known as papaya, is a tropical fruit tree. The fruit is healthy and delicious and the
whole plant parts including fruit, root, bark, peel, seeds and pulp are also known to
have medicinal properties (Vij and Prashar, 2015). Papaya (Carica papaya) can be
al., 2013).
humans and animals that carries scores of pathogens (Geden, et al., 2021). Not
only are house flies a nuisance, but they can also transport disease-causing
organisms. Excessive fly populations are not only an irritant to farm workers but,
when there are nearby human habitations, a public health problem could occur
Control measures for houseflies include sanitation and exclusion, as well as the
waste, proper storage and preparation of food, and regular cleaning of facilities.
Liceo de Cagayan University Senior High School Department
Exclusion measures involve using screens or other barriers to keep flies outside the
Being fast acting, cheap and convenient, insecticides are often the preferred
choice for controlling pests, including house flies. Globally, chemical insecticides
house flies (Wang, et al., 2019). Their effectiveness in controlling pests, ease of
have negative impacts on human health and the environment. Furthermore, most
The study of the efficacy of papaya (Carica papaya) crude latex as a sticky trap
against Musca domestica (the common house fly) is focused on determining the
effectiveness of using the latex from the papaya plant as a natural, alternative
and animals.
of Brgy. Lagtang. And everytime there is an ongoing poultry harvest, this situation
is getting worse because it doesn’t only disturb the people but it also contaminates
foods and may also cause disease due to the pathogens it may bring.
3
alternative component for sticky traps to help minimize their infestation during and
every harvest. The conceptual framework that was used in the study is the “Input-
Process-Output” model as shown in figure 1. The IPO framework was used to show
the process used in making the sticky trap out of papaya (Carica papaya) latex.
classification as to what kind of variable they are. Usually, all the details specified
The process includes the process of how to gather the raw materials and what
will happen afterwards to achieve the sticky trap product, the data collection
For output, this includes the action taken after interpreting the results of the
study. The data analysis will help them determine the level of effectiveness of their
product after all the processes and its gap against the standard sticky traps. From
this, the researchers will conceptualize what could possibly be lacking and what
they can recommend to future researchers who will also conduct similar studies.
1.3 Objectives of the study
The purpose of this study is to make a sticky trap out of papaya latex that will
be helpful for the local people. The following objectives were more specifically
1.3.1 To assess how papaya latex can perform better than standard sticky
traps.
latex.
1.3.3 To identify the length of time needed for the papaya latex to take effect.
insecticides, too much use may cause harm to people. That’s why the researchers
propose their study about using papaya (Carica papaya) crude latex as an
alternative component for sticky trap. Aside from the fact that it is organic, it is
also low-cost and harmless. This study is guided by the following questions:
1.4.1 Is the papaya crude latex effective as a sticky trap to capture house flies
community?
1.4.2 How long will the sticky trap lasts in capturing houseflies (Musca
domestica linnaeus)?
H0: The product of papaya crude latex as a sticky trap is not effective and efficient
in capturing the houseflies that are roaming around in every house of the
community.
of Papaya (Carica papaya) crude latex as an alternative component for sticky trap
against House fly (Musca domestica linnaeus). The study will then be conducted
in order to help the folks listed below. Every family and company that can profit
from our research, like a poultry farm, will gain from it.
Liceo de Cagayan University Senior High School Department
Community. This output will be most beneficial to the community because of the
reason that it can help the community to minimize the houseflies (Musca
this study.
Poultry. The information presented will enable them to act accordingly on this
studies. Because as early as now, through this research study, they will be able to
see the effects, benefits and advantages of being active, diligent and industrious in
consumers may utilize in the future. This will also benefit them to create product
Teachers. The study's findings will help teachers inspire their students to develop
concepts that will give the right direction. Additionally, it might enhance their
Farmers. This study will benefit farmers because it will increase their ability to
papaya) as sticky traps, this area of study can identify parasites that are active at
conducting new researches or in testing the validity of other related findings. This
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study will also serve as their cross reference that will give them a background
overview of the predictors of using the papaya (Carica papaya) crude latex. 7
alternative component to create a sticky trap. The product is intended for use by
limit their study to 5-6 household in this community due to its large population
alone and these households were chosen because it was also the nearest to the
poultry. People living in these households were also the one who are most
The materials that will be used in this study are raw and will be taken from
researchers will conduct their study from the month of March-April because of the
limited time given to them. They plan to put the said sticky trap products during
daytime because that is the time that most flies are active. The residents in the
selected households in the said community will be the one who will evaluate their
product if it is really effective. The sticky trap can only capture little, lightweight
pests like houseflies; that’s why bigger and heavier creatures are beyond their
control.
Aluminum Tray. It refers to the material that will serve as the primary storage of
papaya (Carica papaya) crude latex that is dripping from the fruit.
Cardboard. This will act as the holder of the latex. The latex will then be spread
Houseflies (Musca domestica linnaeus). In this study, this term refers to the main
target of this study. This will determine the efficacy of the organic sticky trap
Papaya (Carica papaya) crude latex. It refers to the primary material to be used
Plastic Container. It also refers to the primary storage of papaya (Carica papaya)
Plastic bottles. It refers to the material where the papaya's (Carica papaya) crude
latex will be transferred from the aluminum tray and plastic container so that it
Yellow Oil Paint. It refers to the material that will make the cardboard yellow for
the reason that the houseflies are attracted to bright colors such as yellow.
Liceo de Cagayan University Senior High School Department
Chapter II
This chapter presents the related literature after the thorough and in-depth
search done by the researchers. Its purpose was to address the problem of the
people living in the community that are experiencing the infestation of houseflies
content and plant species studied, it can be milky white or yellowish, orange to
activity of 2655 units g−1 at pH 5.5 and 285 units g+1 only
With knowledge of the protein composition and protease activity of crude latex,
papaya (Carica papaya) crude latex on the morphological forms of other plants
and invertebrates.
Management (IPM) programs for a variety of greenhouse pests. Many factors have
been identified as influencing whitefly flight behavior and, as a result, trap catches.
discussed. In the literature, only a few YSTs-based action thresholds have been
effort and time associated with counting insects, a method to facilitate species
Vanninen, 2013).
Sticky traps are a standard tool for monitoring alate arthropod pests in
greenhouses, but evaluation of traps over the whole growing season is rarely done.
This study evaluated whether trap densities recommended for practice are
showed that trap catches provide reliable information about pest densities, in
natural enemy activity in the tomato crop. The implementation of these results in
practice and the transferability to other cropping systems are discussed (Böckmann
et al, 2014). 12
As yellow sticky traps (yellow card or plastic covered in a thin layer of clear
glue) or yellow water traps (yellow dishes filled with water and a little detergent)
are well known to be highly attractive to many insects, they can be used for mass
Integrated Pest Management (IPM), mostly for the surveillance of pest aphids,
Aphids, whiteflies, and adult leafminers are just a few of the pests that are
frequently observed with yellow sticky traps. Whiteflies can be captured in large
numbers by using yellow sticky traps in seedling production areas at a rate of 1-2
traps/50-100 m2. Yellow sticky traps significantly reduced the population growth
2018).
One of the most common and widespread insects is the housefly pest on the
kitchen, cattle farm, human and Animal hospitals, slaughterhouses, and other
Liceo de Cagayan University Senior High School Department
facilities. The primary problem caused by houseflies is that they are extremely
bacteria such as E. coli and Salmonella after contact with an animal waste. As a
result, the house fly is responsible for a number of serious diseases in the human
and animal bodies. If at all possible, it is preferable to eliminate the house fly not
only at home, but also in commercial and public places including animal farms,
restaurants, fish market, and a food market processing plant, to name a few.
Observation and controlling house flies in such an open area is more difficult than
methods Control measures have already been implemented. Some cattle farmers
use vacuums to catch flies (Samid, Daud, Mohamed, Mohamad, & Rashid, 2016).
The housefly, Musca domestica, has a long history of association with animals,
many of which are still suffering from parasites. The pest is cosmopolitan in nature
House fly larvae (Musca domestica L.) require a live microbial community to
comprising a suitable substrate for larvae who feed on both the decomposing
communities change as manure ages, and when fly larvae are present changes
attributable to larval grazing also occur. Here, we used high throughput sequencing
of 16S and 18S rRNA genes to characterize microbial communities in dairy cattle
manure and evaluated the changes in those communities over time by comparing
the communities in fresh manure to aged manure with or without house fly larvae
House flies (Musca domestica linnaeus) are widespread, synanthropic filth flies
commonly found on decaying matter, garbage, and feces as well as human food.
They have been shown to vector microbes, including clinically relevant pathogens.
Previous studies have demonstrated that house flies carry a complex and variable
prokaryotic microbiota, but the main drivers underlying this variability and the
differences between the external and internal microbiota and the eukaryotic
House flies (Musca domestica) are common, synanthropic filth flies that may
be found on human food as well as decomposing waste, trash, and feces. They
relevance. Flies carry a broad and rich bacterial community as a result of their
contaminated food are known to be carried by house flies (Nayduch & Burrus,
The following were the most frequently reported bacterial species by several
studies: (a) E. (Blaak, et al., 2014; Poudel, et al., 2019); (b) E. coli, the bacteria
humans; and (c) E. The most significant nosocomial pathogens in hospitals include
bloodstream infections, and urinary tract infections (Selleck et al., 2019); (c) K.
pneumoniae, which also causes post-operative wound infections, can infect the
Houseflies are hardy insects that can endure temperatures between 10 to 45ºC
(50 to 113ºF). A single fly can have 33 million bacteria in its gut and up to 1.9
million bacteria on its body. In humans, several of these infections can lead to
A study by (Sharma, Mayank, & Mal, 2022) stated that yellow sticky traps
(YSTs) may be used in greenhouses to keep an eye on and assess the insect
advantageous time and can be begun at the Economic Injury Level (EIL). Farmers
Liceo de Cagayan University Senior High School Department
can save money by using the simple preparation and application procedures for
YSTs. 16
researchers' study to the people living in the said community. The study of (Puja,
Wardana, Irawan, & Choiron, 2018) explained what papaya (Carica papaya) latex
is while (Macalood, Vicente, Boniao, Gorospe, & Roa, 2013) showed where it can
be collected, and how to acquire it. An example of a standard sticky trap, which is
the YSTs (Pinto-Zevallos & Vanninen, 2013), was also stated by the researchers as
being effective in capturing houseflies. (Nayduch & Burrus, 2017) said that flies
Rashid, 2016) also added that they are extremely bothersome, causing discomfort
to humans and animals, as well as some regurgitation and fecal spots on surfaces.
A study conducted by (Bell, et al., 2019) stated that sticky traps captured more
houseflies than baited sticky pot traps. That's why the researchers are inspired to
make this product because, aside from being organic and low-cost, its material is
also readily available, so everyone will be able to make and use it.
Liceo de Cagayan University Senior High School Department
Chapter III
Research Methodology
use. The purpose of this experimental study is to assess The efficacy of papaya
Liceo de Cagayan University Senior High School Department
To carry out the process for the acquisition of papaya (Carica papaya) crude
latex, materials and equipment that are needed are selected based on their
availability, and the majority of them are derived from the area due to its
abundance. Some materials are also imported from outside the area and are easily
accessible. 18
19
latex sources, which is the papaya, throughout the study will be collected
Collection of latex from papaya (Carica papaya) is based from the study of
(Macalood, Vicente, Boniao, Gorospe, & Roa, 2013) with minor modifications. It
starts by making 6 to 8 longitudinal incisions on the sides of the fruit with a sharp
paring knife or chef's knife. Then, letting the latex drip into the aluminum trays or
plastic containers. The exuded latex was allowed to run down from the fruit and
drip into the collecting devices (aluminum trays, plastic containers) raised in the
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trunk. The collected latex will then be poured into the plastic bottles and spread
In making the yellow cardboard that will be used in making the papaya latex sticky
trap, the researchers will use the flowchart of preparation steps for YSTs from the
study of (Sharma, Mayank, & Mal, 2022) with a few minor modifications.
Material required
Colored sticky traps are utilized in many crops with the intention of mass
median, and mode, to get insight into the typical or central value of our dataset.
In this study, the researchers will use the frequency distribution table to
determine the number of times each value appears in the data set and to identify
any patterns or trends in the distribution. The mean, median, and mode are the
21
use each of these to make generalizations about a population (Dudovskiy, 2022).
The Mean will be used to calculate the average number of flies per day as the
researcher will total all the houseflies collected during that specific day. It will be
∑x
x=
n
where,
N = sum of frequencies
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The Mode will be used to calculate the common numbers of flies per day as the
researchers will also total all the common flies that will be captured during that
( f m −f 1)
Mode = L+h
( f m −f 1) + ( f m −f 2)
where,
22
The Median will be used by the researchers to be able to identify the middle
number of flies when data is arranged in numerical order and to also compare
( n+1 )th
()
th
n
differences. If ‘n’ is odd : Median = term. If ‘n’ is even: Median =
2 2
( )
th
n
term + +1 term. It will be calculated using the following formula:
2
[ ]
n
−F
2
Lm + ⅈ
fm
Where,
Liceo de Cagayan University Senior High School Department
n = Total frequency
i = Class width
This study aims to analyze the efficacy of papaya (Carica papaya) crude latex
linnaeus). The sticky traps made of papaya crude latex will be placed near a
poultry farm in Zone 3, Lagtang, Alubijid, Misamis, Oriental. During the day, the
number of houseflies that will be captured will be recorded daily for a period of
one week. The researchers will also undergo trial and error. The data will then be
number of flies per week, to calculate the common numbers of flies per week, to
identify the middle number of flies when data is arranged in numerical order, and
to also compare differences. It will be measured using the mean, mode, and
median. The mean will be used to calculate the average number of flies per day as
the researcher will total all the houseflies collected during that specific day; the
mode will be used to calculate the common numbers of flies per day as the
researchers will also total all the common flies that will be captured during that
Liceo de Cagayan University Senior High School Department
day; and the median will be used by the researchers to be able to identify the
middle number of flies when data is arranged in numerical order and to also
compare differences.
CHAPTER IV
This chapter discusses the results, analysis, and interpretation of the data
acquired from the responses of respondents who live near poultry farms. It also
contains the outcomes or conclusions of the data collection and analysis. The data
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were given in the tabular form in response to the precise questions posed in the
CHAPTER V
This section presents the findings, conclusions, and recommendations based on the
data analyzed in the previous chapter. The research is summarized, and the study's
conclusions are examined and analyzed. The efficacy of papaya crude latex as an
measuring the extent to which some of the research objectives of the study were
attained.
Liceo de Cagayan University Senior High School Department
Liceo de Cagayan University Senior High School Department
Liceo de Cagayan University Senior High School Department 24
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