3/20/2023

(LAB NO 2)
Sterilization &Disinfectant

Relative Resistance of Microbes

 High resistance
* Bacterial endospores
 Moderate resistance
* protozoa cyst
* Fungal spores
* Naked viruses
 Low resistance
* Vegetative bacteria and fungal cells
* Enveloped viruses

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Definitions
 Sterilization:
*Killing or elimination all forms of microbial life (including endospores) in a material

 Asepsis:
*Absence of significant contamination
 Aseptic techniques:
*are used to prevent contamination of
surgical instruments, medical personnel,
and the patient during surgery

Definitions

 Bacteriostatic Agent:
*An agent that inhibits the growth of bacteria, but does
not necessarily kill them. Suffix stasis: To stop.
 Bactericidal:
*An agent that kills bacteria. Most do not kill
endospores.

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 Sterilization: done by
1- physical Methods
a- heat: include
 moist (Autoclave 121oC for 15 min )
 dry (red heat, direct flaming, incineration and hot air oven)
 Boiling 100oC
 Pasteurization (65oC for 30 minutes)
b- Filtration
c-Radiation

2- Chemical Methods (Disinfection & Antiseptic)

1.Phenols
2. Halogens
3. Alcohols
4. Aldehydes
5. Gaseous Sterilizers

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Filtration technique