ch10

Student: ___________________________________________________________________________

1.  Which of the statements about ribosomal genes is incorrect?  

A.  They have a higher GC content compared to other nuclear genes.
B.  They have a different base composition compared to other nuclear genes.
C.  There are no repetitive sequences.
D.  They are found in the nucleolus.
E.  They are transcribed by RNA polymerases I and III.

2.  You were asked to choose a method to separate a mixture of eukaryotic polymerases. Which of the following
methods would you choose?  
A.  chromatography
B.  western blotting
C.  S1 nuclease assay
D.  DNase 1 footprinting
E.  chromatography and S1 nuclease assay

3.  An antibody was produced and used in an immunohistochemical assay to detect the location of RNA
polymerase I in a thin section of a liver biopsy. The section was stained with the antibody that had been labeled
with a fluorescent tag, then viewed under a fluorescent microscope. State where you would expect to find
positive staining if the enzyme is present.  
A.  rough endoplasmic reticulum
B.  nucleoplasm
C.  nucleolus
D.  endoplasmic reticulum
E.  ribosomes

4.  Which of the following are products of RNA polymerase II activity?  

A.  tRNA
B.  snRNA
C.  hnRNA
D.  tRNA and snRNA
E.  both B. snRNA and C. hnRNA

 
5.  An experiment is planned to study the effect of reducing the transcription of the myosin gene. Which of the
following inhibitors would be useful in this study?  
A.  a-amanitin
B.  DMS
C.  heparin
D.  ammonium sulfate
E.  none of the choices are correct.

6.  Which of the following products are made by RNA polymerase III?  
A.  7SL RNA
B.  5SrRNA
C.  snRNA
D.  both A. 7SL RNA and B. 5SrRNA
E.  7SL RNA, 5SrRNA, and snRNA

7.  A new mutant cell line was developed and found to be defective in polymerase III activity. Which of the
following is likely to be observed in this cell line?  
A.  There will be an overabundance of secreted proteins.
B.  Splicing function is impaired.
C.  There will be an overproduction of 7 SL RNA.
D.  There will be an overabundance of secreted proteins and splicing function is impaired are correct.
E.  There will be an overabundance of secreted proteins, splicing function is impaired, and there will be an
overproduction of 7 SL RNA are correct.

8.  Which of the following is ideal to study the function of the subunits of polymerase II?  
A.  Break the enzymes into component parts and gradually reassemble them.
B.  Use mutational analysis.
C.  Conduct DNA binding assays.
D.  Break the enzymes into component parts and gradually reassemble them and use mutational analysis are
correct.
E.  Use mutational analysis and conduct DNA binding assays are correct.

9.  One effect of the deletion of the hRPB1 subunit of RNA polymerase II is  
A.  loss of the oligosaccharide-binding domain.
B.  inability of polymerase to bind to transcriptional activators.
C.  slowed elongation rate.
D.  non-viable cells.
E.  extended CTD.

 
10.  Which of the following subunits are common in all three yeast polymerases?  
A.  Rpb2
B.  Rpd8
C.  Rpd10
D.  both A. Rpb2 and B. Rpd8
E.  both B. Rpd8 and C. Rpd10

11.  Which of the following is involved in the stress response?  

A.  Rpb2
B.  Rpb3
C.  Rpb4
D.  Rpd8
E.  Rpd10

12.  Which of the following subunits, when combined, are orthologs of the prokaryotic alpha dimer?  
A.  Rpb2 and rpb3
B.  Rpb3 and rpb11
C.  Rpb8 and rpb10
D.  Rpb9 and rpb10
E.  Rpb10 and rpb12

13.  Which of the following would you choose to study the role of polymerase subunits in elongation?  
A.  epitope tagging
B.  immunoprecipitation
C.  in vitro transcription
D.  western blotting
E.  DNAase footprinting

14.  Which of the following is true about the elongation complex?  

A.  The clamp does not come in contact with the RNA:DNA complex.
B.  Processivity is slowed by the closing of the clamp.
C.  The clamp closes over the RNA:DNA hybrid in the enzyme's cleft.
D.  Five loops in the clamps play a role in the movement of the enzyme.
E.  The active site of the enzyme lies at the end of pore 2.

 
15.  Which of the following is true about Rpb4/7?  
A.  It binds to Rpb3 to facilitate melting of the DNA promoter.
B.  It extends the dock region of polymerase, making it easier for transcription factors to bind.
C.  It binds to DNA and facilitate elongation.
D.  It binds to Rpb3 to facilitate melting of the DNA promoter and it extends the dock region of polymerase,
making it easier for transcription factors to bind are correct.
E.  It extends the dock region of polymerase, making it easier for transcription factors to bind and it binds to
DNA and facilitate elongation are correct.

16.  Which of the following is not a part of the core class II promoter?  
A.  TATA box
B.  upstream element
C.  BRE
D.  DPE
E.  Inr

17.  Which of the following techniques would you use to study the role of the BRE?  
A.  S1 nuclease protection analysis
B.  linker scanning mutations
C.  X-ray crystallography
D.  S1 nuclease protection analysis and linker scanning mutations
E.  linker scanning mutations and X-ray crystallography

18.  The CCAAT boxes are bound by which of the following?  

A.  Sp1
B.  DPE
C.  TBP
D.  CTF
E.  TFB

19.  Predict a possible effect of deleting the enhancers region of the polymerase I gene.  
A.  reduced transcription of ribosomes
B.  reduction in the production of most hnRNAs
C.  reduction in the amount of rRNA made
D.  reduction in the production of Rpb1
E.  reduction in the production of Rpb2

20.  Eukaryotic ribosomal RNA genes are transcribed by RNA polymerase II.  
True    False

 
21.  Compared to other nuclear genes, ribosomal genes differ in their base compositions.  
True    False

22.  Roeder and Rutter showed that there are four polymerases operating in the eukaryotic cell.  
True    False

23.  Based on studies on Saccharomyces cerevisiae, there are at least 12 subunits in RNA polymerase II.  
True    False

24.  Epitope tagging is an efficient way to immunoprecipate and isolate an entire polymerase complex.  
True    False

25.  Mutational studies have shown that yeast Rpb3 is homologous to the alpha-subunit of E. coli.  
True    False

26.  The product of the RPB1 gene in yeast assists polymerase IIA in binding DNA.  
True    False

27.  Study of yeast polymerase II has revealed a deep cleft that can accept a linear DNA template from one end
to the other.  
True    False

28.  The catalytic center of polymerase II contains Na+ ions.  

True    False

29.  Enhancers and repressors can be tissue-specific based on the types of DNA-binding proteins that are
present in the cell.  
True    False

 
ch10 Key
 

1.  Which of the statements about ribosomal genes is incorrect?  

A.  They have a higher GC content compared to other nuclear genes.
B.  They have a different base composition compared to other nuclear genes.
C.  There are no repetitive sequences.
D.  They are found in the nucleolus.
E.  They are transcribed by RNA polymerases I and III.

Blooms Level: 1. Remember

Section: 10.01
Weaver - Chapter 10 #1
 

2.  You were asked to choose a method to separate a mixture of eukaryotic polymerases. Which of the following
methods would you choose?  
A.  chromatography
B.  western blotting
C.  S1 nuclease assay
D.  DNase 1 footprinting
E.  chromatography and S1 nuclease assay

Blooms Level: 5. Evaluate

Section: 10.01
Weaver - Chapter 10 #2
 

3.  An antibody was produced and used in an immunohistochemical assay to detect the location of RNA
polymerase I in a thin section of a liver biopsy. The section was stained with the antibody that had been labeled
with a fluorescent tag, then viewed under a fluorescent microscope. State where you would expect to find
positive staining if the enzyme is present.  
A.  rough endoplasmic reticulum
B.  nucleoplasm
C.  nucleolus
D.  endoplasmic reticulum
E.  ribosomes

Blooms Level: 3. Apply

Section: 10.01
Weaver - Chapter 10 #3
 
4.  Which of the following are products of RNA polymerase II activity?  
A.  tRNA
B.  snRNA
C.  hnRNA
D.  tRNA and snRNA
E.  both B. snRNA and C. hnRNA

Blooms Level: 1. Remember

Section: 10.01
Weaver - Chapter 10 #4
 

5.  An experiment is planned to study the effect of reducing the transcription of the myosin gene. Which of the
following inhibitors would be useful in this study?  
A.  a-amanitin
B.  DMS
C.  heparin
D.  ammonium sulfate
E.  none of the choices are correct.

Blooms Level: 2. Understand

Section: 10.01
Weaver - Chapter 10 #5
 

6.  Which of the following products are made by RNA polymerase III?  
A.  7SL RNA
B.  5SrRNA
C.  snRNA
D.  both A. 7SL RNA and B. 5SrRNA
E.  7SL RNA, 5SrRNA, and snRNA

Blooms Level: 1. Remember

Section: 10.01
Weaver - Chapter 10 #6
 

7.  A new mutant cell line was developed and found to be defective in polymerase III activity. Which of the
following is likely to be observed in this cell line?  
A.  There will be an overabundance of secreted proteins.
B.  Splicing function is impaired.
C.  There will be an overproduction of 7 SL RNA.
D.  There will be an overabundance of secreted proteins and splicing function is impaired are correct.
E.  There will be an overabundance of secreted proteins, splicing function is impaired, and there will be an
overproduction of 7 SL RNA are correct.

 
Blooms Level: 3. Apply
Section: 10.01
Weaver - Chapter 10 #7
 

8.  Which of the following is ideal to study the function of the subunits of polymerase II?  
A.  Break the enzymes into component parts and gradually reassemble them.
B.  Use mutational analysis.
C.  Conduct DNA binding assays.
D.  Break the enzymes into component parts and gradually reassemble them and use mutational analysis are
correct.
E.  Use mutational analysis and conduct DNA binding assays are correct.

Blooms Level: 2. Understand

Section: 10.01
Weaver - Chapter 10 #8
 

9.  One effect of the deletion of the hRPB1 subunit of RNA polymerase II is  
A.  loss of the oligosaccharide-binding domain.
B.  inability of polymerase to bind to transcriptional activators.
C.  slowed elongation rate.
D.  non-viable cells.
E.  extended CTD.

Blooms Level: 2. Understand

Section: 10.01
Weaver - Chapter 10 #9
 

10.  Which of the following subunits are common in all three yeast polymerases?  
A.  Rpb2
B.  Rpd8
C.  Rpd10
D.  both A. Rpb2 and B. Rpd8
E.  both B. Rpd8 and C. Rpd10

Blooms Level: 4. Analyze

Section: 10.01
Weaver - Chapter 10 #10
 
11.  Which of the following is involved in the stress response?  
A.  Rpb2
B.  Rpb3
C.  Rpb4
D.  Rpd8
E.  Rpd10

Blooms Level: 1. Remember

Section: 10.01
Weaver - Chapter 10 #11
 

12.  Which of the following subunits, when combined, are orthologs of the prokaryotic alpha dimer?  
A.  Rpb2 and rpb3
B.  Rpb3 and rpb11
C.  Rpb8 and rpb10
D.  Rpb9 and rpb10
E.  Rpb10 and rpb12

Blooms Level: 1. Remember

Section: 10.01
Weaver - Chapter 10 #12
 

13.  Which of the following would you choose to study the role of polymerase subunits in elongation?  
A.  epitope tagging
B.  immunoprecipitation
C.  in vitro transcription
D.  western blotting
E.  DNAase footprinting

Blooms Level: 5. Evaluate

Section: 10.01
Weaver - Chapter 10 #13
 

14.  Which of the following is true about the elongation complex?  

A.  The clamp does not come in contact with the RNA:DNA complex.
B.  Processivity is slowed by the closing of the clamp.
C.  The clamp closes over the RNA:DNA hybrid in the enzyme's cleft.
D.  Five loops in the clamps play a role in the movement of the enzyme.
E.  The active site of the enzyme lies at the end of pore 2.

Blooms Level: 2. Understand

Section: 10.01
Weaver - Chapter 10 #14
 
15.  Which of the following is true about Rpb4/7?  
A.  It binds to Rpb3 to facilitate melting of the DNA promoter.
B.  It extends the dock region of polymerase, making it easier for transcription factors to bind.
C.  It binds to DNA and facilitate elongation.
D.  It binds to Rpb3 to facilitate melting of the DNA promoter and it extends the dock region of polymerase,
making it easier for transcription factors to bind are correct.
E.  It extends the dock region of polymerase, making it easier for transcription factors to bind and it binds to
DNA and facilitate elongation are correct.

Blooms Level: 2. Understand

Section: 10.01
Weaver - Chapter 10 #15
 

16.  Which of the following is not a part of the core class II promoter?  
A.  TATA box
B.  upstream element
C.  BRE
D.  DPE
E.  Inr

Blooms Level: 1. Remember

Section: 10.02
Weaver - Chapter 10 #16
 

17.  Which of the following techniques would you use to study the role of the BRE?  
A.  S1 nuclease protection analysis
B.  linker scanning mutations
C.  X-ray crystallography
D.  S1 nuclease protection analysis and linker scanning mutations
E.  linker scanning mutations and X-ray crystallography

Blooms Level: 5. Evaluate

Section: 10.02
Weaver - Chapter 10 #17
 

18.  The CCAAT boxes are bound by which of the following?  

A.  Sp1
B.  DPE
C.  TBP
D.  CTF
E.  TFB

Blooms Level: 2. Understand

Section: 10.02
Weaver - Chapter 10 #18
 
19.  Predict a possible effect of deleting the enhancers region of the polymerase I gene.  
A.  reduced transcription of ribosomes
B.  reduction in the production of most hnRNAs
C.  reduction in the amount of rRNA made
D.  reduction in the production of Rpb1
E.  reduction in the production of Rpb2

Blooms Level: 4. Analyze

Section: 10.03
Weaver - Chapter 10 #19
 

20.  Eukaryotic ribosomal RNA genes are transcribed by RNA polymerase II.  
FALSE

Blooms Level: 1. Remember

Section: 10.01
Weaver - Chapter 10 #20
 

21.  Compared to other nuclear genes, ribosomal genes differ in their base compositions.  
TRUE

Blooms Level: 1. Remember

Section: 10.01
Weaver - Chapter 10 #21
 

22.  Roeder and Rutter showed that there are four polymerases operating in the eukaryotic cell.  
FALSE

Blooms Level: 1. Remember

Section: 10.01
Weaver - Chapter 10 #22
 

23.  Based on studies on Saccharomyces cerevisiae, there are at least 12 subunits in RNA polymerase II.  
TRUE

Blooms Level: 1. Remember

Section: 10.01
Weaver - Chapter 10 #23
 
24.  Epitope tagging is an efficient way to immunoprecipate and isolate an entire polymerase complex.  
TRUE

Blooms Level: 2. Understand

Section: 10.01
Weaver - Chapter 10 #24
 

25.  Mutational studies have shown that yeast Rpb3 is homologous to the alpha-subunit of E. coli.  
TRUE

Blooms Level: 1. Remember

Section: 10.01
Weaver - Chapter 10 #25
 

26.  The product of the RPB1 gene in yeast assists polymerase IIA in binding DNA.  
TRUE

Blooms Level: 2. Understand

Section: 10.01
Weaver - Chapter 10 #26
 

27.  Study of yeast polymerase II has revealed a deep cleft that can accept a linear DNA template from one end
to the other.  
TRUE

Blooms Level: 2. Understand

Section: 10.01
Weaver - Chapter 10 #27
 

28.  The catalytic center of polymerase II contains Na+ ions.  

FALSE

Blooms Level: 2. Understand

Section: 10.01
Weaver - Chapter 10 #28
 
29.  Enhancers and repressors can be tissue-specific based on the types of DNA-binding proteins that are
present in the cell.  
TRUE

Blooms Level: 2. Understand

Section: 10.03
Weaver - Chapter 10 #29
 
ch10 Summary

Category # of Questions
Blooms Level: 1. Remember 11
Blooms Level: 2. Understand 11
Blooms Level: 3. Apply 2
Blooms Level: 4. Analyze 2
Blooms Level: 5. Evaluate 3
Section: 10.01 24
Section: 10.02 3
Section: 10.03 2
Weaver - Chapter 10 29