10

1. Which of the statements about ribosomal genes is incorrect?

A. T

hey have a higher GC content compared to other nuclear genes.
B. T

hey have a different base composition compared to other nuclear genes.
C. There are no repetitive sequences.

D. They are found in the nucleolus.

E. T
hey are transcribed by RNA polymerases I and III.

2. You were asked to choose a method to separate a mixture of eukaryotic polymerases. Which of
the following methods would you choose?

A. c hromatography

B. w

estern blotting
C. S1 nuclease assay

D. DNase 1 footprinting

E. c hromatography and S1 nuclease assay

3. An antibody was produced and used in an immunohistochemical assay to detect the location of
RNA polymerase I in a thin section of a liver biopsy. The section was stained with the antibody
that had been labeled with a fluorescent tag, then viewed under a fluorescent microscope. State
where you would expect to find positive staining if the enzyme is present.

A. r ough endoplasmic reticulum

B. n

ucleoplasm
C. nucleolus

D. endoplasmic reticulum

E. r ibosomes

4. Which of the following are products of RNA polymerase II activity?

A. t RNA

B. s nRNA

C. hnRNA

D. tRNA and snRNA

E. b oth B. snRNA and C. hnRNA

5. An experiment is planned to study the effect of reducing the transcription of the myosin gene.
Which of the following inhibitors would be useful in this study?

A. α

-amanitin
B. D

MS
C. heparin

D. ammonium sulfate

E. n
one of the choices are correct.

6. Which of the following products are made by RNA polymerase III?

A. 7

SL RNA
B. 5

SrRNA
C. snRNA

D. both A. 7SL RNA and B. 5SrRNA

E. 7
SL RNA, 5SrRNA, and snRNA

7. A new mutant cell line was developed and found to be defective in polymerase III activity. Which
of the following is likely to be observed in this cell line?

A. T

here will be an overabundance of secreted proteins.
B. S

plicing function is impaired.
C. There will be an overproduction of 7 SL RNA.

D. There will be an overabundance of secreted proteins and splicing function is impaired are

correct.
E. T
here will be an overabundance of secreted proteins, splicing function is impaired, and there
will be an overproduction of 7 SL RNA are correct.

8. Which of the following is ideal to study the function of the subunits of polymerase II?

A. B

reak the enzymes into component parts and gradually reassemble them.
B. U

se mutational analysis.
C. Conduct DNA binding assays.

D. Break the enzymes into component parts and gradually reassemble them and use mutational

analysis are correct.
E. U
se mutational analysis and conduct DNA binding assays are correct.


9. One effect of the deletion of the hRPB1 subunit of RNA polymerase II is

A. l oss of the oligosaccharide-binding domain.

B. i nability of polymerase to bind to transcriptional activators.

C. slowed elongation rate.

D. non-viable cells.

E. e xtended CTD.

10. Which of the following subunits are common in all three yeast polymerases?

A. R

pb2
B. R

pd8
C. Rpd10

D. both A. Rpb2 and B. Rpd8

E. b
oth B. Rpd8 and C. Rpd10

11. Which of the following is involved in the stress response?

A. R

pb2
B. R

pb3
C. Rpb4

D. Rpd8

E. R
pd10

12. Which of the following subunits, when combined, are orthologs of the prokaryotic alpha dimer?

A. R

pb2 and rpb3
B. R

pb3 and rpb11
C. Rpb8 and rpb10

D. Rpb9 and rpb10

E. R
pb10 and rpb12


13. Which of the following would you choose to study the role of polymerase subunits in elongation?

A. e

pitope tagging
B. i mmunoprecipitation

C. in vitro transcription

D. western blotting

E. D NAase footprinting

14. Which of the following is true about the elongation complex?

A. T

he clamp does not come in contact with the RNA:DNA complex.
B. P

rocessivity is slowed by the closing of the clamp.
C. The clamp closes over the RNA:DNA hybrid in the enzyme's cleft.

D. Five loops in the clamps play a role in the movement of the enzyme.

E. T
he active site of the enzyme lies at the end of pore 2.

15. Which of the following is true about Rpb4/7?

A. I t binds to Rpb3 to facilitate melting of the DNA promoter.

B. I t extends the dock region of polymerase, making it easier for transcription factors to bind.

C. It binds to DNA and facilitate elongation.

D. It binds to Rpb3 to facilitate melting of the DNA promoter and it extends the dock region of

polymerase, making it easier for transcription factors to bind are correct.
E. I t extends the dock region of polymerase, making it easier for transcription factors to bind and
it binds to DNA and facilitate elongation are correct.

16. Which of the following is not a part of the core class II promoter?

A. T

ATA box
B. u

pstream element
C. BRE

D. DPE

E. I nr


17. Which of the following techniques would you use to study the role of the BRE?

A. S

1 nuclease protection analysis
B. l inker scanning mutations

C. X-ray crystallography

D. S1 nuclease protection analysis and linker scanning mutations

E. l inker scanning mutations and X-ray crystallography

18. The CCAAT boxes are bound by which of the following?

A. S

p1
B. D

PE
C. TBP

D. CTF

E. T
FB

19. Predict a possible effect of deleting the enhancers region of the polymerase I gene.

A. r educed transcription of ribosomes

B. r eduction in the production of most hnRNAs

C. reduction in the amount of rRNA made

D. reduction in the production of Rpb1

E. r eduction in the production of Rpb2

20. Eukaryotic ribosomal RNA genes are transcribed by RNA polymerase II.

True False

21. Compared to other nuclear genes, ribosomal genes differ in their base compositions.

True False

22. Roeder and Rutter showed that there are four polymerases operating in the eukaryotic cell.

True False

23. Based on studies on Saccharomyces cerevisiae, there are at least 12 subunits in RNA
polymerase II.

True False

24. Epitope tagging is an efficient way to immunoprecipate and isolate an entire polymerase
complex.

True False

25. Mutational studies have shown that yeast Rpb3 is homologous to the alpha-subunit of E. coli.

True False

26. The product of the RPB1 gene in yeast assists polymerase IIA in binding DNA.

True False

27. Study of yeast polymerase II has revealed a deep cleft that can accept a linear DNA template
from one end to the other.

True False

28. The catalytic center of polymerase II contains Na+ ions.

True False

29. Enhancers and repressors can be tissue-specific based on the types of DNA-binding proteins that
are present in the cell.

True False

 10 Key

1. Which of the statements about ribosomal genes is incorrect?

A. They have a higher GC content compared to other nuclear genes.

B. They have a different base composition compared to other nuclear genes.

C. There are no repetitive sequences.

D. They are found in the nucleolus.

E. They are transcribed by RNA polymerases I and III.

Blooms Level: 1. Remember

Section: 10.01
Weaver - Chapter 10 #1


2. You were asked to choose a method to separate a mixture of eukaryotic polymerases. Which
of the following methods would you choose?

A. chromatography

B. western blotting

C. S1 nuclease assay

D. DNase 1 footprinting

E. chromatography and S1 nuclease assay

Blooms Level: 5. Evaluate

Section: 10.01
Weaver - Chapter 10 #2


3. An antibody was produced and used in an immunohistochemical assay to detect the location
of RNA polymerase I in a thin section of a liver biopsy. The section was stained with the
antibody that had been labeled with a fluorescent tag, then viewed under a fluorescent
microscope. State where you would expect to find positive staining if the enzyme is present.

A. rough endoplasmic reticulum

B. nucleoplasm

C. nucleolus

D. endoplasmic reticulum

E. ribosomes

Blooms Level: 3. Apply

Section: 10.01
Weaver - Chapter 10 #3



4. Which of the following are products of RNA polymerase II activity?

A. tRNA

B. snRNA

C. hnRNA

D. tRNA and snRNA

E. both B. snRNA and C. hnRNA

Blooms Level: 1. Remember

Section: 10.01
Weaver - Chapter 10 #4


5. An experiment is planned to study the effect of reducing the transcription of the myosin gene.
Which of the following inhibitors would be useful in this study?

A. α-amanitin

B. DMS

C. heparin

D. ammonium sulfate

E. none of the choices are correct.

Blooms Level: 2. Understand

Section: 10.01
Weaver - Chapter 10 #5


6. Which of the following products are made by RNA polymerase III?

A. 7SL RNA

B. 5SrRNA

C. snRNA

D. both A. 7SL RNA and B. 5SrRNA

E. 7SL RNA, 5SrRNA, and snRNA

Blooms Level: 1. Remember

Section: 10.01
Weaver - Chapter 10 #6



7. A new mutant cell line was developed and found to be defective in polymerase III activity.
Which of the following is likely to be observed in this cell line?

A. There will be an overabundance of secreted proteins.

B. Splicing function is impaired.

C. There will be an overproduction of 7 SL RNA.

D. There will be an overabundance of secreted proteins and splicing function is impaired are

correct.
E. There will be an overabundance of secreted proteins, splicing function is impaired, and
there will be an overproduction of 7 SL RNA are correct.

Blooms Level: 3. Apply

Section: 10.01
Weaver - Chapter 10 #7


8. Which of the following is ideal to study the function of the subunits of polymerase II?

A. Break the enzymes into component parts and gradually reassemble them.

B. Use mutational analysis.

C. Conduct DNA binding assays.

D. Break the enzymes into component parts and gradually reassemble them and use

mutational analysis are correct.
E. Use mutational analysis and conduct DNA binding assays are correct.

Blooms Level: 2. Understand

Section: 10.01
Weaver - Chapter 10 #8


9. One effect of the deletion of the hRPB1 subunit of RNA polymerase II is

A. loss of the oligosaccharide-binding domain.

B. inability of polymerase to bind to transcriptional activators.

C. slowed elongation rate.

D. non-viable cells.

E. extended CTD.

Blooms Level: 2. Understand

Section: 10.01
Weaver - Chapter 10 #9



10. Which of the following subunits are common in all three yeast polymerases?

A. Rpb2

B. Rpd8

C. Rpd10

D. both A. Rpb2 and B. Rpd8

E. both B. Rpd8 and C. Rpd10

Blooms Level: 4. Analyze

Section: 10.01
Weaver - Chapter 10 #10


11. Which of the following is involved in the stress response?

A. Rpb2

B. Rpb3

C. Rpb4

D. Rpd8

E. Rpd10

Blooms Level: 1. Remember

Section: 10.01
Weaver - Chapter 10 #11


12. Which of the following subunits, when combined, are orthologs of the prokaryotic alpha
dimer?

A. Rpb2 and rpb3

B. Rpb3 and rpb11

C. Rpb8 and rpb10

D. Rpb9 and rpb10

E. Rpb10 and rpb12

Blooms Level: 1. Remember

Section: 10.01
Weaver - Chapter 10 #12



13. Which of the following would you choose to study the role of polymerase subunits in
elongation?

A. epitope tagging

B. immunoprecipitation

C. in vitro transcription

D. western blotting

E. DNAase footprinting

Blooms Level: 5. Evaluate

Section: 10.01
Weaver - Chapter 10 #13


14. Which of the following is true about the elongation complex?

A. The clamp does not come in contact with the RNA:DNA complex.

B. Processivity is slowed by the closing of the clamp.

C. The clamp closes over the RNA:DNA hybrid in the enzyme's cleft.

D. Five loops in the clamps play a role in the movement of the enzyme.

E. The active site of the enzyme lies at the end of pore 2.

Blooms Level: 2. Understand

Section: 10.01
Weaver - Chapter 10 #14


15. Which of the following is true about Rpb4/7?

A. It binds to Rpb3 to facilitate melting of the DNA promoter.

B. It extends the dock region of polymerase, making it easier for transcription factors to bind.

C. It binds to DNA and facilitate elongation.

D. It binds to Rpb3 to facilitate melting of the DNA promoter and it extends the dock region of

polymerase, making it easier for transcription factors to bind are correct.
E. It extends the dock region of polymerase, making it easier for transcription factors to bind
and it binds to DNA and facilitate elongation are correct.

Blooms Level: 2. Understand

Section: 10.01
Weaver - Chapter 10 #15



16. Which of the following is not a part of the core class II promoter?

A. TATA box

B. upstream element

C. BRE

D. DPE

E. Inr

Blooms Level: 1. Remember

Section: 10.02
Weaver - Chapter 10 #16


17. Which of the following techniques would you use to study the role of the BRE?

A. S1 nuclease protection analysis

B. linker scanning mutations

C. X-ray crystallography

D. S1 nuclease protection analysis and linker scanning mutations

E. linker scanning mutations and X-ray crystallography

Blooms Level: 5. Evaluate

Section: 10.02
Weaver - Chapter 10 #17


18. The CCAAT boxes are bound by which of the following?

A. Sp1

B. DPE

C. TBP

D. CTF

E. TFB

Blooms Level: 2. Understand

Section: 10.02
Weaver - Chapter 10 #18



19. Predict a possible effect of deleting the enhancers region of the polymerase I gene.

A. reduced transcription of ribosomes

B. reduction in the production of most hnRNAs

C. reduction in the amount of rRNA made

D. reduction in the production of Rpb1

E. reduction in the production of Rpb2

Blooms Level: 4. Analyze

Section: 10.03
Weaver - Chapter 10 #19


20. Eukaryotic ribosomal RNA genes are transcribed by RNA polymerase II.

FALSE

Blooms Level: 1. Remember
Section: 10.01
Weaver - Chapter 10 #20


21. Compared to other nuclear genes, ribosomal genes differ in their base compositions.

TRUE

Blooms Level: 1. Remember
Section: 10.01
Weaver - Chapter 10 #21


22. Roeder and Rutter showed that there are four polymerases operating in the eukaryotic cell.

FALSE

Blooms Level: 1. Remember
Section: 10.01
Weaver - Chapter 10 #22


23. Based on studies on Saccharomyces cerevisiae, there are at least 12 subunits in RNA
polymerase II.

TRUE

Blooms Level: 1. Remember
Section: 10.01
Weaver - Chapter 10 #23


24. Epitope tagging is an efficient way to immunoprecipate and isolate an entire polymerase
complex.

TRUE

Blooms Level: 2. Understand
 Section: 10.01
Weaver - Chapter 10 #24


25. Mutational studies have shown that yeast Rpb3 is homologous to the alpha-subunit of E. coli.

TRUE

Blooms Level: 1. Remember
Section: 10.01
Weaver - Chapter 10 #25


26. The product of the RPB1 gene in yeast assists polymerase IIA in binding DNA.

TRUE

Blooms Level: 2. Understand
Section: 10.01
Weaver - Chapter 10 #26


27. Study of yeast polymerase II has revealed a deep cleft that can accept a linear DNA template
from one end to the other.

TRUE

Blooms Level: 2. Understand
Section: 10.01
Weaver - Chapter 10 #27


28. The catalytic center of polymerase II contains Na+ ions.

FALSE

Blooms Level: 2. Understand
Section: 10.01
Weaver - Chapter 10 #28


29. Enhancers and repressors can be tissue-specific based on the types of DNA-binding proteins
that are present in the cell.

TRUE

Blooms Level: 2. Understand
Section: 10.03
Weaver - Chapter 10 #29

 10 Summary

Category # of Questions
Blooms Level: 1. Remember 11
Blooms Level: 2. Understand 11
Blooms Level: 3. Apply 2
Blooms Level: 4. Analyze 2
Blooms Level: 5. Evaluate 3
Section: 10.01 24
Section: 10.02 3
Section: 10.03 2
Weaver - Chapter 10 29