BIOCHEMISTRY 14.

Which is the final step in the post

LONG QUIZ 1 - BIOCHEMISTRY transcriptional processing?
1ST SEM | A.Y. 2023-2024 | NOVEMBER 28,2023 a. ATP binds to both the cap and on the
PROF. RALPH CYLON JACINTO poly A tail
b.
1. Responsible for attaching short sequences of c.
nucleotides which serves as the starting point of d.
DNA replication
ANS: RNA Primase 15. Which is the correct pair:
Ans: TATA box - mRNA
2. Joins the okazaki fragments
Ans: DNA Ligase 16. What is true of snoRNA
a.
3. Responsible for relieving torsion stress b. derived from coding of introns
Ans: DNA Topoisomerase c.
d.
4. Proofreads the newly synthesized DNA strands
Ans: DNA Polymerase 17. What is the role of the promoter in regulating
gene expression?
5. Unwinds the double helix A. Allow for alternative splicing
Ans: DNA Helicase B. Alter gene expression by binding
transcription factors
6. Responsible for synthesizing new DNA strand: C. Bind negative regulators to inhibit gene
a. DNA Polymerase expression
b. DNA Ligase D. Recruit RNA Polymerase and
c. DNA Helicase Transcription factors
d. RNA Polymerase
e. DNA Topoisomerase 18. All are methods for termination of transcription
in prokaryotes except:
7. Responsible for DNA Repair A. Hairloop Pin
Answer: DNA polymerase B. Release Binding
C. Rho Mediated Termination
8-11. 5’- ACAGGGTTTA - 3’ D. None of the above
3’ - TGTCCCAAAT - 5’
8. Which is the leading strand 19. What event is indicative of transcription
Answer: 5’- ACAGGGTTTA - 3’ initiation
a. RNA polymerase bind to promoter
9. Which is the lagging strand? b. RNA polymerase removed from DNA
Answer: 3’ - TGTCCCAAAT - 5’ C. Telomerase lengthens telomeres
D. RNA strands begin to be synthesized
10. Which is the Okazaki fragment?
Answer: 3’ - TGTCCCAAAT - 5’ 20. What elements do all eukaryotic promoters
region
11. What is the mRNA based on the given strand? a. Basal Promoter (TATA Box)
Answer: 3’ - UGUCCCAAAU - 5’ b. Enhancers
c. Transcription Factors
12. Factors responsible for the termination site d. All of the above
Ans: Rho Factor
Sigma factor - starting/initiation site 21. This glycogen storage diseases results to an
increased uric acid production due to an
13. Short lifespan of a normal mRNA is determined increased R5P production via HMP pathway
by what repeating sequences a. Mc Ardle
Ans: Repeated U sequences (since it is on b. Von Gierkes
mRNA) c. Lesch-Nyhan Syndrome
d. Gout
22. What is the enzyme deficient? (Von Gierkes)
a.
b. Glucose-6-phosphatase
c.
d.

23. Which of the following is associated with

hypourecemia?
A. Lesch-Nyhan syndrome
B. Overactivity of PRPP synthase
C. Overactivity of amidotranferase
D. Adenosine deaminase deficiency

24. Rate limiting step of Pyrimidine biosynthesis

Ans. Aspartate transcarbamoylase

25. Feedback inhibited by both purine and

pyrimidine
Ans. PRPP synthase

26. Which of the following cofactors is used

during the conversion of uracil to thymine?
Ans. Tetrahydrofolate

27. Methotrexate inhibits which of the ff enzymes?

Ans. Dihydrofolate reductase

28. Degradation product of pyrimidine biosynthesis

a. Allantoin
b. Beta-alanine
c. Uric Acid
d. Succinyl-CoA

29. Which of the ff drug is an alternative substrate

for OPRT, intake of which may precipitate
Orotic aciduria.
Answer: Allopurinol

30. 7-year old boy is diagnosed with Lesch-Nyhan.

Which of the following enzymes is the
problem?
Answer: HGPRT

31. Swollen base of 1st metatarsophalyngeal joint

Ans. Podagra

32. Non modifiable risk factor of gouty arthritis

A. Alcoholism
B. Non-vegetarian diet
C. Family history
D. None of the above