NAME:_____________________________ Date: ________ SCORE:__________

Lab. teacher: ___________________ Course & Section_________ Grp. No.______

ACTIVITY NO. 3
ALCOHOLIC FERMENTATION
Results:

Original
bubble
Sample 15 30 45 60 75 90
length
(mm)
9 mm 15 mm 45 mm 70 mm 75 mm 75 mm
Glucose
0 mm

Galactos 2 mm 2 mm 5 mm 5 mm 8 mm 9 mm
e 0 mm

8 mm 20 mm 36 mm 63 mm 65 mm 65 mm
Fructose
0 mm

1 mm 1 mm 1 mm 1 mm 1 mm 1 mm
Xylose
0 mm

3 mm 5 mm 7 mm 11 mm 16 mm 21 mm
Maltose
0 mm

0 mm 0 mm 0 mm 1 mm 1 mm 1 mm 1 mm
Lactose

0 mm 10 mm 23 mm 40 mm 77 mm 80 mm 82 mm
Sucrose

0 mm 0 mm 0 mm 1 mm 2 mm 2 mm 2 mm
Starch
Question Guide:
1. Define fermentation.
Fermentation is a metabolic process in which an organism converts a carbohydrates, such
as strach or a sugar, into am alcohol or an acid. For instance, in order to produce energy,
yeast uses fermentation to transform sugar into alcohol In biochemistry, it is specially
described as the process of obtaining energy from carbohydrates without the presence
oxygen. The word "ferment" derived from the Latin word " fervere" which means "to
boil".
2. What are the products of fermentation?

Although fermentation can result in a variety of products, the most common ones are
ethanol, lactic acid, carbon dioxide, and hydrogen gas (H2). Commercial applications for
these materials include use in food, vitamins, medications, and industrial chemicals.

3. Which of the sample/s is/are fermented with difficulty? Which of the sample/s is/are
easily fermented? Which are not fermented at all?

Fermented w/ difficulty Easily fermented Not fermented at all

Galactose Glucose Lactose
Sucrose Starch
Fructose Xylose
Maltose

4. Write the balance equation in the above reaction.

5.
How much energy is given off in the reaction?
Two ATP molecules are released during the alcohol fermentation process through
glycolysis. In the overall reaction for glycolysis, one molecule of glucose is converted to
two molecules of pyruvic acid. Along the way, two molecules of adenosine diphosphate
(ADP) are phosphorylated to ATP, and two molecules of NAD (the oxidized form of
NAD, or nicotinamide adenine dinucleotide) are reduced to NADH. ATP serves as an
energy carrier and can be used to power many cellular processes. The NADH carries
high-energy electrons, which can be used to produce more ATP by chemiosmosis.
Alcoholic Fermentation The net energy gain in fermentation is 2 ATP molecules/glucose
molecule. In both lactic acid and alcoholic fermentation, all the NADH produced in
glycolysis is consumed in fermentation, so there is no net NADH production, and no
NADH to enter the ETC and form more ATP.