Aconitase is an enzyme that catalyses the stereo-specific isomerization of citrate to

isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process.

1. (Aconitate hydratase; EC 4.2.1.3)

2. Primary sequence-

3.  Aconitase contains a Fe4S4 cluster which converts to Fe3S4 when the

enzyme is inactive.

FIGURE2: Functional 3D structure of cis-Aconitase

4. Aconitase consists of four domains, three of which are tightly packed while the
fourth is more flexible. Aconitase contains a 4Fe-4S iron-sulfur cluster. This
iron sulfur cluster does not participate in redox as most do, but holds the OH
group of citrate to facilitate its elimination. It is at this 4Fe-4S site that catalysis
occurs and citrate or isocitrate is bound.
5. PDB file number of cis-Aconitase is 1B0J
6. The cis-Aconitase enzyme belong to the class of lysase
7.