STUDY THE EFFECT OF KI ON FLUORESCENCE EFFICIENCY

OF PYRENESULPHONIC ACID (PSA) & DETERMINATION OF

STERN-VOLMER QUENCHING CONSTANT

AIM
To study effect of KI on fluorescence efficiency of PSA & determine Stern-Volmer quenching
constant

MATERIALS REQUIRED
Sodium salt of pyrenesulphonic acid, potassium iodide, distilled water, micropipette, beakers,
and fluorescence spectrometer with cuvette cells etc.

OBSERVATIONS AND CALCULATIONS

Concentration of stock solution of PSA = 10 -6 M
Concentration of stock solution of KI = 10-4 M

SL NO. vol of vol of vol of KI(mL) [KI] (µM) Fluorescence Φof/Φf

PSA KI(µL) area
(mL) Φf

1 2 0 0 0 11874.86419 1

2 2 25 0.025 0.833333 12573.69904 0.944421

3 2 100 0.1 3.333333 12143.86439 0.977849

4 2 200 0.2 6.666667 11903.38432 0.997604

5 2 250 0.25 8.333333 11241.98352 1.056296

6 2 300 0.3 10 11153.31614 1.064694

7 2 350 0.35 11.66667 11464.28757 1.035814

8 2 400 0.4 13.33333 11303.59412 1.050539

9 2 500 0.5 16.66667 11129.12548 1.067008

Here, [KI] is calculated by M1V1 = M2V2 where V1 =Vol of KI(mL) ; V2 = 3mL ; M1 = 10-4 M
and M2 = [KI].
Absorption and Emission Spectra
 Fig10. The stern-volmer spectra Φof / Φf vs the concentration of quencher [KI](μM).
# NOTE : spectra are plotted in ORIGIN PRO 2021 and scatter plot in XLSTAT 2018.
Now the experimental data points obtained to the curve y(x) = (slope).x+1 or y = 0.0064x + 1
(here, intercept = 0.9713≈1) which can be compared with the stern-volmer relationship equation,
Φof / Φf = [ Kq / ( Kf + Knr ) ][KI] + 1
Or
o
Φ f / Φf = [ KSV ][KI] + 1
Where, KSV = Stern-Volmer quenching constant

From comparison we get that the slope of the graph is equal to KSV .

So, Stern-Volmer quenching constant=0.0064μM-1 .

RESULTS