Part Question Full Marks

Paper 1 50 50
4551/2 Name:_________________________ Paper A No.1 12
Form 4 Biology Class: 4S____ 2 No.2 12
No.3 13
Paper 2 B No.4 33
MAY 2016 C No.5 20
2 1/2 hours No.6 20
No.7 20
No.8 20
P1+P2 Total/160
Total/95
Topical Test 2 5/5
Final Mark
FIRST TERM EXAMINATION 2016
SMK ST. ANTHONY
2 HOURS 30 MINUTES

This question paper consists of three sections: Section A, B and Section C.

You are advised to spend 50 minutes to answer questions in Section A, 50 minutes for Section B and 50 minutes for
Section C.
Section A : Structured Question [37 marks].
Answer all the questions.

2. Diagram 2.1 shows two levels of organization in protein structure.

Level of organization in protein X: __________________________________________________

Level of organization in protein Y: __________________________________________________

Diagram 2.1

(a) In Diagram 2.1, name the level of organization in the protein structures of X and Y. [2m]

(b) Describe the structure of protein X. [2m]

______________________________________________________________________________________________

______________________________________________________________________________________________

______________________________________________________________________________________________

(c) Diagram 2.2 shows how protein X is broken down to form products.

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 Diagram 2.2

Explain how the products in Diagram 2.2 are formed. [2m]

______________________________________________________________________________________________

______________________________________________________________________________________________

______________________________________________________________________________________________

(d) State why animal proteins are first class protein.[1m]

_____________________________________________________________________________________________

(e)
Silk is an example of protein. A silk garment crumples when it is washed using hot water
at temperature of 65oC.

(i) Explain the above statement.[3m]

______________________________________________________________________________________________

______________________________________________________________________________________________

______________________________________________________________________________________________

(ii) Based on the above statement, suggest two ways to maintain the quality of silk garments. [2m]
1. ________________________________________________________________________________________

________________________________________________________________________________________

2. ________________________________________________________________________________________

________________________________________________________________________________________

3. Diagram 3.1 shows three stages of mitosis, P, Q and R, in an animal cell.

Diagram 3.1

a) Name the stages P, Q and R in the spaces provided in Diagram 3.1. [3m]
b) Explain what happens at stage Q. [3m]
_______________________________________________________________________________________
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 _______________________________________________________________________________________

_______________________________________________________________________________________

c) State the chromosome behavior at the following stages:

Stage P: [1m]
_______________________________________________________________________________________

Stage Q: [1m]
_______________________________________________________________________________________

d) Explain the importance of mitosis in the process of cloning orchid plants. [3m]
______________________________________________________________________________________

_______________________________________________________________________________________

_______________________________________________________________________________________

e) Diagram 3.2 shows a cell at stage P. In the next stage, it is found that chromosome M is not separated.

Diagram 3.2

Complete the diagram for two daughter cells which will be formed in the space provided below. [2m]

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SECTION B: PRACTICAL ASSESSMENT [33 marks]

Answer all the questions

4. An experiment was carried out to investigate the effect of pH values on the hydrolysis of starch by amylase enzyme.
Several buffer solutions with different pH values were prepared.

The following steps were carried out.

Step 1: 2 ml of 1% amylase solution was placed into a boiling tube containing 5 ml of buffer solution at pH 6.
Step 2: 2 drops of iodine solution was placed into each groove on a white tile
Step 3: 3 ml of 1% of starch solution was added into a boiling tube and the stopwatch is started immediately.
Step 4: Every 2 minutes, a drop of the mixture from the boiling tube was dropped into a new groove of iodine solution
on the white tile by using a clean dropper.
Step 5: The time taken for the iodine solution to remain yellow is recorded.
Step 6: Steps 1-5 are repeated using buffer solution at pH 5, 7, 8 and 9.

Diagram 4 shows the materials and apparatus used in this experiment for different pH values of buffer solutions.

Diagram 4.1
Diagram 4.2 shows the observation for the experiment using buffer solution at pH 6 after 6 minutes.

Diagram 4.2
(a) In Table 1, list all the materials and apparatus labelled in Diagram 4.1 [3 marks]
Material Apparatus

Table 4.3 shows the results of this experiment.

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pH of buffer solution Observation Time taken for iodine solution to
End of experiment remain yellow (min)

5 ______ minutes

6 minutes

7
2 minutes

______ minutes

_______ minutes

Table 4.3

(b) Record the time taken for iodine solution to remain yellow in Table 4.3. [3 marks]
(c) (i) State two different observations made from Table 4.3. [3 marks]

Observation 1:_______________________________________________________________________________
___________________________________________________________________________________________
___________________________________________________________________________________________
___________________________________________________________________________________________

Observation 2: ______________________________________________________________________________
___________________________________________________________________________________________
___________________________________________________________________________________________
__________________________________________________________________________________________
(ii) State the inferences from observations in 4 (c) (i). [3 marks]
Inference from observation 1:

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 ___________________________________________________________________________________________
___________________________________________________________________________________________
___________________________________________________________________________________________
___________________________________________________________________________________________
Inference from observation 2:
___________________________________________________________________________________________
___________________________________________________________________________________________
___________________________________________________________________________________________
__________________________________________________________________________________________

(d) Complete Table 4.4 based from this experiment. [3 marks]

Variable Method to handle the variable
Manipulated variable:

Responding variable:

Constant variable:

Table 4.4
(e) State the hypothesis for this experiment. [3 marks]
___________________________________________________________________________________________
___________________________________________________________________________________________
___________________________________________________________________________________________
___________________________________________________________________________________________

(f) (i) Construct a table and record all the data collected in this experiment. Your table should have the following
titles:
 pH
 Time taken for iodine solution to remain yellow
 Rate of amylase activity on starch

Rate of amylase activity = _________________1_____________________

Time taken for iodine solution to remain yellow
[3 marks]

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 (ii) Using the data in 4 (f) (i), draw a graph of the rate of amylase activity on starch against the pH values of the
mixture solution. [3 marks]

(g) Based from graph in 4 (f) (ii), explain the relationship between the rate of amylase activity on starch and the
pH value of the mixture solution. [3 marks]
________________________________________________________________________________________
________________________________________________________________________________________
________________________________________________________________________________________
_______________________________________________________________________________________

(h) State the operational definition for the hydrolysis of starch by amylase enzyme. [3 marks]
________________________________________________________________________________________
________________________________________________________________________________________
________________________________________________________________________________________
________________________________________________________________________________________

(i) This experiment is repeated using buffer solution at pH 7 in water bath at 20°C. Predict the outcome of this
experiment. Explain your prediction. [3 marks]
________________________________________________________________________________________
________________________________________________________________________________________
________________________________________________________________________________________
________________________________________________________________________________________

SECTION C: [20 marks] Answer one question only.

5. (a) (i) What is the meaning of osmosis? [2 marks]
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 (ii) In Diagram 5.1 shows the level of two sucrose solutions of different concentrations in the arms of U-tube at
the beginning of an experiment. Draw a diagram to show the levels of the U-tube at the end of experiment.
[2 marks]

Diagram 5.1
(iii) Explain the process that occurs in the (a) ii. [6 marks]

(b) Two experiments are conducted to study two processes. Diagram 5.2 (a) and Diagram 5.2 (b) show the apparatus
set-up at the beginning of the experiments.

Diagram 5.2
(i) What are the two processes studied in Diagram 5.2 (a) and Diagram 5.2 (b) called? [2 marks]
(ii) Draw two diagrams, one to show the distribution of bromine molecules in the gas jar and the other to show
the levels of the solutions in the thistle funnel and the beaker. [3 marks]

(c) Give three similarities and two differences between simple diffusion and osmosis [5 marks]

6. (a) The activity of an enzyme may be affected by chemicals called the inhibitors. Outline five other characteristics
of enzymes. [10 marks]
(b) Use diagrams to elaborate on the ‘lock and key’ hypothesis of enzyme action on substrate. [4 marks]
(c) Enzymes are used in many applications. For instance, protease is used to tenderise meat. Outline the
applications of three of the following enzymes: amylase, cellulose, lipase, trypsin, zymase. [6 marks]

END OF QUESTION PAPER

Section A prepared Section B&C prepared by: Checked by: Verified by:
___________ __________________ __________________ _________________
Ms. Yii Ee Hua Mdm Dyg Hadzar A. Solhi Ms. Irene Lue Mdm. Wendy Tiong
Subject Teacher Subject Teacher Head of Bio Panel Head of Sc and Maths Department