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  • 0 - Lemper Isolasi Enzim 5 Kamiss

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    ffaaiiqq ggrreeaat
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    1. The document describes calculations to determine glucose concentration and enzyme activity for 4 variables over time periods of 10, 20, 30, and 40 minutes. 2. Glucose concentration is calculated using the titration method by measuring initial and final volumes and applying a formula. 3. Enzyme activity is calculated using the change in glucose concentration over time, sample mass, and molecular weight constants in a standard formula. 4. Glucose concentration increases over time for each variable as the enzyme hydrolyzes the substrate, while enzyme activity levels off around 2-2.5 units/mol.min by 40 minutes.

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    0_Lemper Isolasi Enzim 5 Kamiss

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    1. The document describes calculations to determine glucose concentration and enzyme activity for 4 variables over time periods of 10, 20, 30, and 40 minutes. 2. Glucose concentration is calculated using the titration method by measuring initial and final volumes and applying a formula. 3. Enzyme activity is calculated using the change in glucose concentration over time, sample mass, and molecular weight constants in a standard formula. 4. Glucose concentration increases over time for each variable as the enzyme hydrolyzes the substrate, while enzyme activity levels off around 2-2.5 units/mol.min by 40 minutes.

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    © All Rights Reserved
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    2 views10 pages

    0 - Lemper Isolasi Enzim 5 Kamiss

    Uploaded by

    ffaaiiqq ggrreeaat
    1. The document describes calculations to determine glucose concentration and enzyme activity for 4 variables over time periods of 10, 20, 30, and 40 minutes. 2. Glucose concentration is calculated using the titration method by measuring initial and final volumes and applying a formula. 3. Enzyme activity is calculated using the change in glucose concentration over time, sample mass, and molecular weight constants in a standard formula. 4. Glucose concentration increases over time for each variable as the enzyme hydrolyzes the substrate, while enzyme activity levels off around 2-2.5 units/mol.min by 40 minutes.

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    of 10

    LEMBAR PERHITUNGAN

     Sebelum Inkubasi
    Perhitungan Kadar Glukosa (C)
    F= 22,2 ml
    1. Variabel 1
    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal
    20 ml 25 ml
    (22,2 ml−19 ml)× ×
    = 5 ml 5 x 2.5
    20 ml
    = 8 mg/ml
    2. Variabel 2

    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal

    20 ml 25 ml
    (22,2 ml−18,9 ml)× ×
    = 5 ml 5 x 2.5
    20 ml

    = 8,25 mg/ml

    3. Variabel 3

    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal

    20 ml 25 ml
    (22,2 ml−18,5 ml)× ×
    = 5 ml 5 x 2.5
    20 ml

    = 9,25 mg/ml

    4. Variabel 4

    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal
    20 ml 25 ml
    (22,2 ml−17,9 ml)× ×
    = 5 ml 5 x 2.5
    20 ml

    = 10,75 mg/ml

     Setelah Inkubasi
    F = 23,3 ml
    1. Variabel 1
    a. t = 10 menit
    Perhitungan Kadar Glukosa (C)
    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal
    20 ml 25 ml
    (23,3 ml−18,9 ml)× ×
    = 5 ml 5 x 2.5
    20 ml
    = 11 mg/ml
    Perhitungan Aktivitas Enzim (AE)

    ΔC 1000 1 unit
    T × BM × 1 μmol
    Aktivitas enzim =

    mg 1000
    (11−8) 1 unit
    = ml ×
    180
    gr ×
    1 μmol
    10 menit mol
    = 1,6 unit/mol.menit
    b. t = 20 menit
    Perhitungan Kadar Glukosa (C)
    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal
    20 ml 25 ml
    (23,3 ml−17,2 ml)× ×
    = 5 ml 5 x 2.5
    20 ml
    = 15,25 mg/ml
    Perhitungan Aktivitas Enzim (AE)
    ΔC 1000 1 unit
    T × BM × 1 μmol
    Aktivitas enzim =

    mg 1000
    ( 15,25−8 ) 1 unit
    = ml ×
    180
    gr ×
    1 μmol
    20 menit mol
    = 2,01 unit/mol.menit
    c. t = 30 menit

    Perhitungan Kadar Glukosa (C)

    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal

    20 ml 25 ml
    (23,3 ml−15,4 ml)× ×
    = 5 ml 5 x 2.5
    20 ml

    = 19,75 mg/ml

    Perhitungan Aktivitas Enzim (AE)

    ΔC 1000 1 unit
    T × BM × 1 μmol
    Aktivitas enzim =

    mg 1000
    (19,75−8) 1 unit
    = ml ×
    180
    gr ×
    1 μmol
    3 0 menit mol
    = 2,18 unit/mol.menit

    d. t = 40 menit

    Perhitungan Kadar Glukosa (C)

    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal

    20 ml 25 ml
    (23,3 ml−13,6 ml)× ×
    = 5 ml 5 x 2.5
    20 ml

    = 24,25 mg/ml
    Perhitungan Aktivitas Enzim (AE)

    ΔC 1000 1 unit
    T × BM × 1 μmol
    Aktivitas enzim =

    mg 1000
    (24,25−8) 1 unit
    = ml ×
    180
    gr ×
    1 μmol
    4 0 menit mol
    = 2,27 unit/mol.menit

    2. Variabel 2
    a. t = 10 menit
    Perhitungan Kadar Glukosa (C)
    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal
    20 ml 25 ml
    (23,3 ml−18,9 ml)× ×
    = 5 ml 5 x 2.5
    20 ml
    = 11 mg/ml
    Perhitungan Aktivitas Enzim (AE)

    ΔC 1000 1 unit
    T × BM × 1 μmol
    Aktivitas enzim =

    mg 1000
    (11−8,25) 1 unit
    = ml ×
    180
    gr ×
    1 μmol
    10 menit mol
    = 1,53 unit/mol.menit
    b. t = 20 menit
    Perhitungan Kadar Glukosa (C)
    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal
    20 ml 25 ml
    (23,3 ml−16,9 ml)× ×
    = 5 ml 5 x 2.5
    20 ml
    = 16 mg/ml
    Perhitungan Aktivitas Enzim (AE)
    ΔC 1000 1 unit
    T × BM × 1 μmol
    Aktivitas enzim =

    mg 1000
    ( 16−8,25 ) 1 unit
    = ml ×
    180
    gr ×
    1 μmol
    2 0 menit mol
    = 2,15 unit/mol.menit
    c. t = 30 menit

    Perhitungan Kadar Glukosa (C)

    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal

    20 ml 25 ml
    (23,3 ml−15,3 ml)× ×
    = 5 ml 5 x 2.5
    20 ml

    = 20 mg/ml

    Perhitungan Aktivitas Enzim (AE)

    ΔC 1000 1 unit
    T × BM × 1 μmol
    Aktivitas enzim =

    mg 1000
    (20−8,25) 1 unit
    = ml ×
    180
    gr ×
    1 μmol
    3 0 menit mol
    = 2,18 unit/mol.menit

    d. t = 40 menit

    Perhitungan Kadar Glukosa (C)

    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal

    20 ml 25 ml
    (23,3 ml−12,9 ml)× ×
    = 5 ml 5 x 2.5
    20 ml
    = 26 mg/ml

    Perhitungan Aktivitas Enzim (AE)

    ΔC 1000 1 unit
    T × BM × 1 μmol
    Aktivitas enzim =

    mg 1000
    ( 26−8,25 ) 1 unit
    = ml ×
    180
    gr ×
    1 μmol
    4 0 menit mol
    = 2,47 unit/mol.menit
    3. Variabel 3
    a. t = 10 menit
    Perhitungan Kadar Glukosa (C)
    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal
    20 ml 25 ml
    (23,3 ml−16,6 ml)× ×
    = 5 ml 5 x 2.5
    20 ml
    = 16,75 mg/ml
    Perhitungan Aktivitas Enzim (AE)

    ΔC 1000 1 unit
    T × BM × 1 μmol
    Aktivitas enzim =

    mg 1000
    ( 16,75−9,25 ) 1 unit
    = ml ×
    180
    gr ×
    1 μmol
    10 menit mol
    = 4,16 unit/mol.menit
    b. t = 20 menit
    Perhitungan Kadar Glukosa (C)
    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal
    20 ml 25 ml
    (23,3 ml−15,9 ml)× ×
    = 5 ml 5 x 2.5
    20 ml
    = 18,5 mg/ml
    Perhitungan Aktivitas Enzim (AE)

    ΔC 1000 1 unit
    T × BM × 1 μmol
    Aktivitas enzim =

    mg 1000
    (18,5−9,25) 1 unit
    = ml ×
    180
    gr ×
    1 μmol
    2 0 menit mol
    = 2,57 unit/mol.menit
    c. t = 30 menit

    Perhitungan Kadar Glukosa (C)

    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal

    20 ml 25 ml
    (23,3 ml−15,1 ml)× ×
    = 5 ml 5 x 2.5
    20 ml

    = 20,5 mg/ml

    Perhitungan Aktivitas Enzim (AE)

    ΔC 1000 1 unit
    T × BM × 1 μmol
    Aktivitas enzim =

    mg 1000
    ( 20,5−9,25 ) 1 unit
    = ml ×
    180
    gr ×
    1 μmol
    3 0 menit mol
    = 2,04 unit/mol.menit

    d. t = 40 menit

    Perhitungan Kadar Glukosa (C)

    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal
    20 ml 25 ml
    (23,3 ml−14,1 ml)× ×
    = 5 ml 5 x 2.5
    20 ml

    = 23 mg/ml

    Perhitungan Aktivitas Enzim (AE)

    ΔC 1000 1 unit
    T × BM × 1 μmol
    Aktivitas enzim =

    mg 1000
    (23−9,25) 1 unit
    = ml ×
    180
    gr ×
    1 μmol
    4 0 menit mol
    = 1,91 unit/mol.menit
    4. Variabel 4
    a. t = 10 menit
    Perhitungan Kadar Glukosa (C)
    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal
    20 ml 25 ml
    (23,3 ml−15,9 ml)× ×
    = 5 ml 5 x 2.5
    20 ml
    = 18,5 mg/ml
    Perhitungan Aktivitas Enzim (AE)

    ΔC 1000 1 unit
    T × BM × 1 μmol
    Aktivitas enzim =

    mg 1000
    (18,5−10,75) 1 unit
    = ml ×
    180
    gr ×
    1 μmol
    10 menit mol
    = 4,31 unit/mol.menit
    b. t = 20 menit
    Perhitungan Kadar Glukosa (C)
    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal
    20 ml 25 ml
    (23,3 ml−15,2 ml)× ×
    = 5 ml 5 x 2.5
    20 ml
    = 20,25 mg/ml
    Perhitungan Aktivitas Enzim (AE)

    ΔC 1000 1 unit
    T × BM × 1 μmol
    Aktivitas enzim =

    mg 1000
    (20,25−10,75) 1 unit
    = ml ×
    180
    gr ×
    1 μmol
    2 0 menit mol
    = 2,64 unit/mol.menit
    c. t = 30 menit

    Perhitungan Kadar Glukosa (C)

    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal

    20 ml 25 ml
    (23,3 ml−14,3 ml)× ×
    = 5 ml 5 x 2.5
    20 ml

    = 22,5 mg/ml

    Perhitungan Aktivitas Enzim (AE)

    ΔC 1000 1 unit
    T × BM × 1 μmol
    Aktivitas enzim =

    mg 1000
    (22,5−10,75) 1 unit
    = ml ×
    180
    gr ×
    1 μmol
    3 0 menit mol
    = 2,18 unit/mol.menit

    d. t = 40 menit

    Perhitungan Kadar Glukosa (C)

    Vtotal Vpengenceran
    ( F−M )× ×
    C= Vtitrasi Vdiambil x 2.5
    Vtotal
    20 ml 25 ml
    (23,3 ml−13,4 ml)× ×
    = 5 ml 5 x 2.5
    20 ml

    = 24,75 mg/ml

    Perhitungan Aktivitas Enzim (AE)

    ΔC 1000 1 unit
    T × BM × 1 μmol
    Aktivitas enzim =

    mg 1000
    (24,75−10,75) 1 unit
    = ml ×
    180
    gr ×
    1 μmol
    4 0 menit mol
    = 1,94 unit/mol.menit

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