CHLORIDE

Ref : CHLO-0250 12 x 20 mL
Instruction for use : CHLO-0600 2 x 125 mL
Convertion Factor : mEq/L x 1 = mmol/L

URINARY SAMPLE PREPARATION

Urinary sample must be diluted 1/2 with distilled water before analysis. The results must be multiplied by 2.

APPLICATION Selectra Pro Serie & Flexor EL Serie

General Sample types
Name CHLORIDE Normal
Abbreviated name CHLO Allowed Yes
Code 260 Predilution No 0

Mode End point monochromatic Reference values

Wavelength 505 nm 0 Male XXX - XXX mEq/L
Test parameters 1

Units mEq/L Female XXX - XXX mEq/L

Decimals 1 Pediatric XXX - XXX mEq/L
Test repeats 1 Panic XXX - XXX mEq/L
Controls
Control 1 XXX
Concentration limits 10.0 - 130.0 mEq/L Control 2 XXX
Calibrator name XXX Control 3 XXX
Correlation
Prozone check No Correlation factor 1.000
Correlation offset 0.000 mEq/L

General Measurement
Name CHLORIDE Slope Blank No 0 0
P dil ti
Predilution N
No 0 IIncubation
b ti titime 4.5
45 min
i 0
Sample
Sample blank No 0 0
normal volume rerun volume* Factor 1.00
Test parameters 2

3.0 µL 2.0 µl Absorbance limits

Reagents Absorbance 0.000 - 0.800 Abs
Reagent blank Yes 3 R. Absorbance 0.000 - 0.200 Abs
normal volume rerun volume*
Reagent addition at - 2.25 minutes (R1) 0 9.000 0
300 µL 301 µl
Reagent addition at 2.90 minutes (R2) 0
0 µL 0 µl 0 9.000 0 9.000 0
Reagent addition at 4.70 minutes (R3) 0 9.000 0 9.000 0
0 µL 0 µl 0 9.000 0

Calibrator Predilution
Name XXX Auto predilution No
Number of standards 1 0
Calibration accepted - 0 0
Calibration

Repeats 3 Cut-off
Interval 7 days Cut-off No
Curvefit 0
0 0 0
0 0 0 9.000 9.000
Auto accept calibration Yes 0 9.000 9.000

N° Used Concentration 0 0
#1 Standard #1 XXX 0 FAUX
0 0 FAUX
0 0 FAUX XXX: Enter data by the user
0 0 FAUX * : For information only
0 0 FAUX
0 0 FAUX
0 0 FAUX (01/2011)
AA-PRO/ELserie--CHLO-1
 Modification from the previous version 1/2
 CHLORIDE
Ref : CHLO-0250 12 x 20 mL
Instruction for use : CHLO-0600 2 x 125 mL
Convertion Factor : mEq/L x 1 = mmol/L

URINARY SAMPLE PREPARATION

Urinary sample must be diluted 1/2 with distilled water before analysis. The results must be multiplied by 2.

APPLICATION Selectra Pro Serie & Flexor EL Serie

0 0 FAUX

PERFORMANCE DATA
Analytical range
The reagent is linear from 10 to 130 mEq/L on serum/plasma and from 10 to 250 mEq/L on urine.
Detection limit
Determined according to SFBC protocol, the detection limit is equal to 2 mEq/L on serum/plasma and urine.
Sensitivity
The average variation of the analytical signal is 3.85x10-3∆A per mEq/L of chloride (3.85x10-3∆A per mmol/L) for a light path of 1 cm.

Precision
Serum / plasma Within Run Between Run
N Mean
CV% Mean (mEq/L) CV%
mEq/L (mEq/L)
Level 1 20 97.2 0.9% 96.3 1.0%
Level 2 20 113.2 0.9% 111.4 1.3%
Urine Within Run Between Run
N Mean
CV% Mean ((mEq/L)
q ) CV%
mEq/L (mEq/L)
Level 1 20 30.3 0.5% 32.7 2.3%
Level 2 20 63.8 0.3% 81.8 2.1%
Level 3 20 151.8 0.3% 145.2 2.0%
Correlation
A comparative study has been performed on 35 human serum/plasma samples.
The sample concentrations were between 50.3 and 132.5 mEq/L.
The linear regression parameters are the follows:
Correlation coefficient : (r) = 0.997
Linear regression : y= 0.942x + 3.3 mEq/L
Interferences
According to SFBC recommendations, some studies have been performed to determine the level of interference
from different compounds:
Serum / plasma
Turbidity :Positive bias from 300 mg/dL Triglycerides equivalent.
Hemoglobin: Positive bias from 250 mg/dL.
Conjugated bilirubin :No significant interference up to 25 mg/dL.
Unconjugated bilirubin :No significant interference up to 36 mg/dL on normal serum and positive bias from 23 mg/dL
on pathological serum.
Other compounds may interfere (see technical insert).
Urine
Hemoglobin: Positive bias from 300 mg/dL.
Conjugated bilirubin :No significant interference up to 25 mg/dL.
Other compounds may interfere (see technical insert).
On board stability / Calibration frequency (refrigerated position)
On-board stability: 28 days
Calibration frequency: 7 days
Make a new calibration when reagent lots change, when quality control results fall outside the established range and after
a maintenance operation.
(01/2011)
AA-PRO/ELserie--CHLO-1
 Modification from the previous version 2/2