Document number: 841104_K_applic_COCAINE, Date: 02/04, s.w. 6.

0
Test definition COCAINE Calibr. parameters
Test type Photometric Test in use YES Calibration type NONLINEAR
Full name Cocaine Low High Units Bias corr. in use NO
metabolite
Online name Test limit 0 1000 µg/l Repeat time (d) 1
Result Unit µg/l low( Ö ), high (!) Abs. error (mA) 5
Point/Calibrator Duplicate
Number of decimals 0 Initial absorbance 0 2 A Rel. error (%) 0
Acceptance MANUAL
Acceptance AUTOMATIC Response limit
(mA)
Type of calibrator SEPARATE Min. *
Dilution 1 + 0 Dilution limit (!) * * µg/l Calibrator Conc.(!) Dil. Ratio. 1+ Max. *
Sample type DoA Cal 0 0 0
Secondary dil. 1+ 0 0 DoA Cal B2 300 0
¨Serum ¨Plasma ýUrine DoA Cal B4 1000 0
¨C.S.F ¨Other Correction factor 1
More
Correction bias 0

Test flow Reagent definition

Blank NO Fixed cuvette
Antigen excess NO

Syringe BCR Id. Stable

speed on board
Name (days)
Curve type
Incubation Incubation
Reagent Volume (µl) Time (sec.) Reagent Time (sec.) λ 1 (nm) NONLINEAR
COCA1 4 300 COCA2 120 340 Nonlinearity
Conc.(µg/l)

Volume (µl) Disp. with Volume (µl) λ 2 (nm) 50 COCA1 Slow 409 30
55 WATER 55 NONE %

Disp. with Volume (µl) Disp. with 20 COCA2 Slow 410 30

Extra with 10 Extra with
buffers buffers Time (sec)
180
Volume (µl) Dilution with Volume (µl)
8 WATER 8
Points & Inter.

Wash reagent Wash reagent Wash reagent Konelab 6/36

60/30/20XT
NONE [WATER] NONE

Konelab™ COCAINE METABOLITE - code: 981624 (2 x 30 ml) for cut off 300 µg/l
Reagent: Liquids, ready-to-use. It is recommended to store the reagents tightly sealed at 2...8 °C when not in use.
Calibrator: DoA Negative Cal., 981720, (DoA Calibrator B1, 981724), DoA Calibrator B2, 981725, (DoA Calibrator B3, 981726), DoA Calibrator B4, 981727.
Liquids, ready to use.

Q.C. sample: DoA Control set B, 981728. Liquids, ready to use.