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Document number: 841101_I_applic_BARB, Date: 02/04, s.w. 6.

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Test definition BARB Calibr. parameters
Test type Photometric Test in use YES Calibration type NONLINEAR
Full name Barbiturates Low High Units Bias corr. in use NO
Online name Test limit 0 1000 µg/l Repeat time (d) 7
Result Unit µg/l low( Ö ), high (!) Abs. error (mA) 5
Point/Calibrator Duplicate
Number of decimals 0 Initial absorbance 0 2 A Rel. error (%) 0
Acceptance MANUAL
Acceptance AUTOMATIC Response limit
(mA)
Type of calibrator SEPARATE Min. *
Dilution 1 + 0 Dilution limit (!) * * µg/l Calibrator Conc.(!) Dil. Ratio. 1+ Max. *
Sample type DoA Cal 0 0 0
Secondary dil. 1+ 0 0 DoA Cal B2 200 0
¨Serum ¨Plasma ýUrine DoA Cal B4 1000 0
¨C.S.F ¨Other Correction factor 1
More
Correction bias 0

Test flow Reagent definition


Blank NO Fixed cuvette
Antigen excess NO

Syringe BCR Id. Stable


speed on board
(days)
Name
Curve type
Incubation Incubation
Reagent Volume (µl) Time (sec.) Reagent Time (sec.) λ 1 (nm) NONLINEAR
BARB1 5 300 BARB2 120 340 Nonlinearity
Conc.(µg/l)

Volume (µl) Disp. with Volume (µl) λ 2 (nm) 30 BARB1 Slow 403 30
55 WATER 55 NONE %

Disp. with Volume (µl) Disp. with 10 BARB2 Slow 404 30


Extra with 10 Extra with
buffers buffers Time (sec)
90
Volume (µl) Dilution with Volume (µl)
8 WATER 8
Points & Inter.

Wash reagent Wash reagent Wash reagent Konelab 6/18


60/30/20XT
NONE [WATER] NONE

Konelab™ BARBITURATES - code: 981621 (2 x 30 ml) for cut off 200 µg/l

Reagent: Liquids, ready-to-use. It is recommended to store the reagents tightly sealed at 2...8 °C when not in use.
Calibrator: DoA Negative Cal., 981720, (DoA Calibrator B1, 981724), DoA Calibrator B2, 981725, (DoA Calibrator B3, 981726), DoA Calibrator B4, 981727.
Liquids, ready to use.

Q.C. sample: DoA Control set B, 981728. Liquids, ready to use.

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