Document number: 841032_G_applic_Apo A1, Date: 02/04, s.w. 6.

0
Test definition APO A1 Calibr. parameters
Test type Photometric Test in use YES Calibration type NONLINEAR
Full name Apolipoprotein Low High Units Bias corr. in use NO
A1
Online name Test limit 0.1 18.7 g/l Repeat time (d) 14
Result Unit g/l low( Ö ), high (!) Abs. error (mA) 5
Point/Calibrator Single
Number of decimals 2 Initial absorbance 0 2 A Rel. error (%) 2
Acceptance MANUAL
Acceptance AUTOMATIC Response limit
(mA)
Type of calibrator SERIES Min. *
Dilution 1 + 10 Dilution limit (!) 0.22 4.03 g/l Calibrator Conc.(!) Dil. Ratio. 1+ Max. *
Sample type Apo Cal 1.1 55
Secondary dil. 1+ 2 50 Apo Cal 1.1 27
ýSerum ýPlasma ¨Urine Apo Cal 1.1 14
¨C.S.F ¨Other Correction factor 1 Apo Cal 1.1 7
More Apo Cal 1.1 4
Correction bias 0 Apo Cal 1.1 2

Test flow Reagent definition

Blank YES Normal cuvette
Antigen excess NO

Syringe BCR Stable

speed Id. on board
Sample & Name (days)
dilution
Incubation Incubation
Reagent Volume (µl) Raw S. with Time (sec.) Blank Reagent Time (sec.) λ 1 (nm)
APO BUFFER 6 WATER 200 APO A1 AS 420 340

Volume (µl) Disp. with Volume (µl) Resp. min (A) Volume (µl) λ 2 (nm) APO Normal 204 30
BUFFER
110 EXTRA 30 * 30 NONE

Disp. with Volume (µl) Special dil. Resp. max (A) Disp. with SPEDIL Normal 201 30
EXTRA 20 SPEDIL 0.1 WATER

Volume (µl) Dilution with Disp. with Volume (µl) APO A1 AS Normal 232 30
20 Special dil. EXTRA 10

Wash reagent Wash reagent Volume (µl) Wash reagent Meas. Type
NONE NONE 20 NONE NORMAL

Konelab™ APOLIPOPROTEIN A1 - code 981662

Reagent: Liquids, ready-to-use.
Calibrator: Included in the kit. Add 1.00 ml distilled water and wait 30 min before use. Stability: 2 months at 2...8 °C after reconstitution.
! Values are lot dependent, refer to value sheet and General Information for calculation.
Ö determination limit.

Q.C. sample: LipoTrol 981653. Reconstitute with 3.00 ml of distilled water. Stable for 5 days at 2...8 °C.