Document number: 841030_H_applic_AAT, Date: 02/04, s.w. 6.

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Test definition AAT Calibr. parameters
Test type Photometric Test in use YES Calibration type NONLINEAR
Full name Alpha1- Low High Units Bias corr. in use NO
Antitrypsin
Online name Test limit 0.1 20.57 g/l Repeat time (d) 14
Result Unit g/l low( Ö ), high (!) Abs. error (mA) 5
Point/Calibrator Single
Number of decimals 2 Initial absorbance 0 2 A Rel. error (%) 2
Acceptance MANUAL
Acceptance AUTOMATIC Response limit
(mA)
Type of calibrator SERIES Min. *
Dilution 1 + 10 Dilution limit (!) 0.24 4.44 g/l Calibrator Conc.(!) Dil. Ratio. 1+ Max. *
Sample type SpeciCal 1.21 55
Secondary dil. 1+ 2 50 SpeciCal 1.21 27
ýSerum ýPlasma ¨Urine SpeciCal 1.21 14
¨C.S.F ¨Other Correction factor 1 SpeciCal 1.21 7
More SpeciCal 1.21 4
Correction bias 0 SpeciCal 1.21 2

Test flow Reagent definition

Blank YES Normal cuvette
Antigen excess NO

Syringe BCR Stable

speed Id. on board
Sample & Name (days)
dilution
Incubation Incubation
Reagent Volume (µl) Raw S. with Time (sec.) Blank Reagent Time (sec.) λ 1 (nm)
BUFFER 4 WATER 200 AAT AS 420 340

Volume (µl) Disp. with Volume (µl) Resp. min (A) Volume (µl) λ 2 (nm) BUFFER Normal 202 30
115 EXTRA 30 * 30 NONE

Disp. with Volume (µl) Special dil. Resp. max (A) Disp. with SPEDIL Normal 201 30
EXTRA 20 SPEDIL 0.1 WATER

Volume (µl) Dilution with Disp. with Volume (µl) AAT AS Normal 238 30
20 Special dil. EXTRA 10

Wash reagent Wash reagent Volume (µl) Wash reagent Meas. Type
NONE NONE 20 NONE NORMAL

Konelab™ ALPHA-1-ANTITRYPSIN - code: 981661

Reagent: Liquids, ready-to-use.

Calibrator: SpeciCal 980997, liquid ready to use. Stability: 3 months at 2...8 °C after opening.
! Values are lot dependent, refer to SpeciCal value sheet.
Ö determination limit.

Q.C. sample: SpeciTrol 981250 & SpeciTrol High 981799, liquid ready to use. Stability: 3 months 2...8 °C after opening.